Restriction map for
pFN21AB6388
(Linear) (Six-base) MAPSORT of: KIBB6388.seq Check: 7388 from: 1 to: 903
KIBB6388.orf
With 182 enzymes: SgfI *
October 18, 2013 09:44 ..
AccI GT'mk_AC
Cuts at: 0 38 903
Size: 38 865
Acc65I G'GTAC_C
Cuts at: 0 838 903
Size: 838 65
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 903
Size: 289 614
AfeI AGC'GCT
Cuts at: 0 658 903
Size: 658 245
AflIII A'CryG_T
Cuts at: 0 662 903
Size: 662 241
AlwNI CAG_nnn'CTG
Cuts at: 0 485 903
Size: 485 418
ApaI G_GGCC'C
Cuts at: 0 437 903
Size: 437 466
ApaLI G'TGCA_C
Cuts at: 0 598 903
Size: 598 305
AvaI C'yCGr_G
Cuts at: 0 75 402 903
Size: 75 327 501
Fragments arranged by size:
501 327 75
BamHI G'GATC_C
Cuts at: 0 488 903
Size: 488 415
BanI G'GyrC_C
Cuts at: 0 277 406 838 903
Size: 277 129 432 65
Fragments arranged by size:
432 277 129 65
BanII G_rGCy'C
Cuts at: 0 437 641 856 903
Size: 437 204 215 47
Fragments arranged by size:
437 215 204 47
Bme1580I G_kGCm'C
Cuts at: 0 280 409 437 602 903
Size: 280 129 28 165 301
Fragments arranged by size:
301 280 165 129 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 431 903
Size: 431 472
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 817 903
Size: 817 86
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 364 547 812 903
Size: 232 132 183 265 91
Fragments arranged by size:
265 232 183 132 91
Bpu10I CC'TnA_GC
Cuts at: 0 688 903
Size: 688 215
BsaAI yAC'GTr
Cuts at: 0 663 903
Size: 663 240
BsaHI Gr'CG_yC
Cuts at: 0 502 592 903
Size: 502 90 311
Fragments arranged by size:
502 311 90
BsaWI w'CCGG_w
Cuts at: 0 856 903
Size: 856 47
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 337 367 520 550 903
Size: 337 30 153 30 353
Fragments arranged by size:
353 337 153 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 304 903
Size: 304 599
BseYI C'CCAG_C
Cuts at: 0 478 903
Size: 478 425
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 208 903
Size: 6 202 695
Fragments arranged by size:
695 202 6
BsiEI CG_ry'CG
Cuts at: 0 889 903
Size: 889 14
BsiHKAI G_wGCw'C
Cuts at: 0 602 903
Size: 602 301
BsmI GAATG_Cn'
Cuts at: 0 751 903
Size: 751 152
Bsp1286I G_dGCh'C
Cuts at: 0 280 409 437 602 641 856 903
Size: 280 129 28 165 39 215 47
Fragments arranged by size:
280 215 165 129 47 39 28
BspEI T'CCGG_A
Cuts at: 0 856 903
Size: 856 47
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 559 903
Size: 559 344
BsrFI r'CCGG_y
Cuts at: 0 368 841 888 903
Size: 368 473 47 15
Fragments arranged by size:
473 368 47 15
BssSI C'ACGA_G
Cuts at: 0 49 903
Size: 49 854
BstAPI GCAn_nnn'nTGC
Cuts at: 0 20 903
Size: 20 883
BstEII G'GTnAC_C
Cuts at: 0 546 735 903
Size: 546 189 168
Fragments arranged by size:
546 189 168
BstXI CCAn_nnnn'nTGG
Cuts at: 0 525 903
Size: 525 378
BstYI r'GATC_y
Cuts at: 0 488 903
Size: 488 415
BtgI C'CryG_G
Cuts at: 0 623 903
Size: 623 280
EaeI y'GGCC_r
Cuts at: 0 886 903
Size: 886 17
EagI C'GGCC_G
Cuts at: 0 886 903
Size: 886 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 289 364 547 812 903
Size: 232 57 75 183 265 91
Fragments arranged by size:
265 232 183 91 75 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 346 903
Size: 346 557
EcoO109I rG'GnC_Cy
Cuts at: 0 86 396 445 903
Size: 86 310 49 458
Fragments arranged by size:
458 310 86 49
FspI TGC'GCA
Cuts at: 0 577 903
Size: 577 326
HaeII r_GCGC'y
Cuts at: 0 660 903
Size: 660 243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 337 369 520 552 903
Size: 337 32 151 32 351
Fragments arranged by size:
351 337 151 32 32
KpnI G_GTAC'C
Cuts at: 0 842 903
Size: 842 61
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 450 563 903
Size: 450 113 340
Fragments arranged by size:
450 340 113
MslI CAynn'nnrTG
Cuts at: 0 820 903
Size: 820 83
MspA1I CmG'CkG
Cuts at: 0 70 273 309 831 903
Size: 70 203 36 522 72
Fragments arranged by size:
522 203 72 70 36
NaeI GCC'GGC
Cuts at: 0 370 903
Size: 370 533
NgoMIV G'CCGG_C
Cuts at: 0 368 903
Size: 368 535
NspI r_CATG'y
Cuts at: 0 582 903
Size: 582 321
PflMI CCAn_nnn'nTGG
Cuts at: 0 485 903
Size: 485 418
PfoI T'CCnGG_A
Cuts at: 0 209 341 903
Size: 209 132 562
Fragments arranged by size:
562 209 132
PpuMI rG'GwC_Cy
Cuts at: 0 86 445 903
Size: 86 359 458
Fragments arranged by size:
458 359 86
PspOMI G'GGCC_C
Cuts at: 0 433 903
Size: 433 470
PstI C_TGCA'G
Cuts at: 0 152 830 903
Size: 152 678 73
Fragments arranged by size:
678 152 73
SanDI GG'GwC_CC
Cuts at: 0 86 903
Size: 86 817
SfcI C'TryA_G
Cuts at: 0 148 826 903
Size: 148 678 77
Fragments arranged by size:
678 148 77
StuI AGG'CCT
Cuts at: 0 259 903
Size: 259 644
StyI C'CwwG_G
Cuts at: 0 392 512 903
Size: 392 120 391
Fragments arranged by size:
392 391 120
TatI w'GTAC_w
Cuts at: 0 215 903
Size: 215 688
XmnI GAAnn'nnTTC
Cuts at: 0 124 903
Size: 124 779
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AlwNI ApaI ApaLI
AvaI BamHI BanI BanII Bme1580I BmrI BplI BpmI
Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrFI BssSI
BstAPI BstEII BstXI BstYI BtgI EaeI EagI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I KpnI MmeI MslI
MspA1I NaeI NgoMIV NspI PflMI PfoI PpuMI PspOMI
PstI SanDI SfcI StuI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AhdI AleI AlfI
AloI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BpuEI BsaI BsaBI BsiWI BsmBI
BspHI BsrBI BsrDI BsrGI BssHII BstBI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI
EciI EcoICRI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI KasI MfeI MluI MscI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI XmaI ZraI