(Linear) (Six-base) MAPSORT of: KIBB6316.seq  Check: 8324  from: 1  to: 843



KIBB6316.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:43  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     843 
   Size:         76     767
 
ApoI r'AATT_y

Cuts at:      0      45     215     315     843 
   Size:         45     170     100     528

  Fragments arranged by size: 

                 528     170     100      45
 
AseI AT'TA_AT

Cuts at:      0     386     843 
   Size:        386     457
 
AvaI C'yCGr_G

Cuts at:      0      56     843 
   Size:         56     787
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     237     503     629     843 
   Size:        237     266     126     214

  Fragments arranged by size: 

                 266     237     214     126
 
BglII A'GATC_T

Cuts at:      0     355     534     660     843 
   Size:        355     179     126     183

  Fragments arranged by size: 

                 355     183     179     126
 
Bpu10I CC'TnA_GC

Cuts at:      0      11     843 
   Size:         11     832
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     389     559     685     843 
   Size:        389     170     126     158

  Fragments arranged by size: 

                 389     170     158     126
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      31     843 
   Size:         31     812
 
BseYI C'CCAG_C

Cuts at:      0     550     676     843 
   Size:        550     126     167

  Fragments arranged by size: 

                 550     167     126
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265     843 
   Size:        265     578
 
BstYI r'GATC_y

Cuts at:      0     355     534     660     843 
   Size:        355     179     126     183

  Fragments arranged by size: 

                 355     183     179     126
 
BtsI GCAGTG_nn'

Cuts at:      0     439     843 
   Size:        439     404
 
ClaI AT'CG_AT

Cuts at:      0     252     843 
   Size:        252     591
 
DraI TTT'AAA

Cuts at:      0     171     843 
   Size:        171     672
 
EarI CTCTTCn'nnn_

Cuts at:      0      62     276     843 
   Size:         62     214     567

  Fragments arranged by size: 

                 567     214      62
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     843 
   Size:         76     767
 
EcoRI G'AATT_C

Cuts at:      0     215     843 
   Size:        215     628
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     594     626     843 
   Size:        594      32     217

  Fragments arranged by size: 

                 594     217      32
 
MslI CAynn'nnrTG

Cuts at:      0     561     687     843 
   Size:        561     126     156

  Fragments arranged by size: 

                 561     156     126
 
NspI r_CATG'y

Cuts at:      0     442     843 
   Size:        442     401
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      70     102     843 
   Size:         70      32     741

  Fragments arranged by size: 

                 741      70      32
 
PstI C_TGCA'G

Cuts at:      0     461     843 
   Size:        461     382
 
SapI GCTCTTCn'nnn_

Cuts at:      0     276     843 
   Size:        276     567
 
SfcI C'TryA_G

Cuts at:      0     327     457     843 
   Size:        327     130     386

  Fragments arranged by size: 

                 386     327     130
 
SmlI C'TyrA_G

Cuts at:      0     404     538     664     843 
   Size:        404     134     126     179

  Fragments arranged by size: 

                 404     179     134     126
 
SspI AAT'ATT

Cuts at:      0     412     843 
   Size:        412     431
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     191     843 
   Size:        191     652
 
XmnI GAAnn'nnTTC

Cuts at:      0      71     413     843 
   Size:         71     342     430

  Fragments arranged by size: 

                 430     342      71
 

 Enzymes that do cut:

     AcuI     ApoI     AseI     AvaI     BbsI    BglII   Bpu10I    BpuEI
    BseRI    BseYI     BsgI    BstYI     BtsI     ClaI     DraI     EarI
  Eco57MI    EcoRI    Hin4I     MslI     NspI     PsrI     PstI     SapI
     SfcI     SmlI     SspI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI    CspCI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI