Restriction map for
pFN21AB6293
(Linear) (Six-base) MAPSORT of: KIBB6293.seq Check: 3531 from: 1 to: 2418
KIBB6293.orf
With 182 enzymes: SgfI *
July 3, 2012 16:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 492 2346 2418
Size: 492 1854 72
Fragments arranged by size:
1854 492 72
AatII G_ACGT'C
Cuts at: 0 584 650 2418
Size: 584 66 1768
Fragments arranged by size:
1768 584 66
AflIII A'CryG_T
Cuts at: 0 413 2264 2418
Size: 413 1851 154
Fragments arranged by size:
1851 413 154
AhdI GACnn_n'nnGTC
Cuts at: 0 93 1620 2418
Size: 93 1527 798
Fragments arranged by size:
1527 798 93
AleI CACnn'nnGTG
Cuts at: 0 2248 2418
Size: 2248 170
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2185 2215 2217 2247 2418
Size: 2185 30 2 30 171
Fragments arranged by size:
2185 171 30 30 2
AlwNI CAG_nnn'CTG
Cuts at: 0 141 171 252 687 974 2226 2370 2418
Size: 141 30 81 435 287 1252 144 48
Fragments arranged by size:
1252 435 287 144 141 81 48 30
ApoI r'AATT_y
Cuts at: 0 1458 2418
Size: 1458 960
AvaI C'yCGr_G
Cuts at: 0 467 533 1302 1735 2004 2418
Size: 467 66 769 433 269 414
Fragments arranged by size:
769 467 433 414 269 66
BamHI G'GATC_C
Cuts at: 0 44 1307 2418
Size: 44 1263 1111
Fragments arranged by size:
1263 1111 44
BanI G'GyrC_C
Cuts at: 0 1318 2418
Size: 1318 1100
BanII G_rGCy'C
Cuts at: 0 308 545 901 2057 2418
Size: 308 237 356 1156 361
Fragments arranged by size:
1156 361 356 308 237
BbsI GAAGACnn'nnnn_
Cuts at: 0 861 2418
Size: 861 1557
BbvCI CC'TCA_GC
Cuts at: 0 25 121 1084 1688 2418
Size: 25 96 963 604 730
Fragments arranged by size:
963 730 604 96 25
BciVI GTATCCnnnnn_n'
Cuts at: 0 1868 2418
Size: 1868 550
BclI T'GATC_A
Cuts at: 0 1530 2418
Size: 1530 888
BglII A'GATC_T
Cuts at: 0 764 1491 2418
Size: 764 727 927
Fragments arranged by size:
927 764 727
Bme1580I G_kGCm'C
Cuts at: 0 1321 2301 2418
Size: 1321 980 117
Fragments arranged by size:
1321 980 117
BmgBI CAC'GTC
Cuts at: 0 557 963 1446 2418
Size: 557 406 483 972
Fragments arranged by size:
972 557 483 406
BmrI ACTGGGnnnn_n'
Cuts at: 0 1236 2418
Size: 1236 1182
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 315 2418
Size: 315 2103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 624 709 753 2418
Size: 592 32 85 44 1665
Fragments arranged by size:
1665 592 85 44 32
Bpu10I CC'TnA_GC
Cuts at: 0 25 121 1084 1688 2418
Size: 25 96 963 604 730
Fragments arranged by size:
963 730 604 96 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2125 2418
Size: 2125 293
BsaI GGTCTCn'nnnn_
Cuts at: 0 775 2418
Size: 775 1643
BsaAI yAC'GTr
Cuts at: 0 150 594 1374 2418
Size: 150 444 780 1044
Fragments arranged by size:
1044 780 444 150
BsaHI Gr'CG_yC
Cuts at: 0 581 647 2134 2418
Size: 581 66 1487 284
Fragments arranged by size:
1487 581 284 66
BsaWI w'CCGG_w
Cuts at: 0 1826 2418
Size: 1826 592
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 304 334 343 373 2185 2215 2245 2418
Size: 304 30 9 30 1812 30 30 173
Fragments arranged by size:
1812 304 173 30 30 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 589 1936 1939 1942 2209 2418
Size: 130 459 1347 3 3 267 209
Fragments arranged by size:
1347 459 267 209 130 3 3
BseYI C'CCAG_C
Cuts at: 0 819 1096 1839 2072 2418
Size: 819 277 743 233 346
Fragments arranged by size:
819 743 346 277 233
BsiEI CG_ry'CG
Cuts at: 0 380 886 1392 1624 2152 2249 2418
Size: 380 506 506 232 528 97 169
Fragments arranged by size:
528 506 506 380 232 169 97
BsiHKAI G_wGCw'C
Cuts at: 0 275 308 2418
Size: 275 33 2110
Fragments arranged by size:
2110 275 33
BsmBI CGTCTCn'nnnn_
Cuts at: 0 418 915 2191 2418
Size: 418 497 1276 227
Fragments arranged by size:
1276 497 418 227
Bsp1286I G_dGCh'C
Cuts at: 0 275 308 545 901 1321 2057 2301 2418
Size: 275 33 237 356 420 736 244 117
Fragments arranged by size:
736 420 356 275 244 237 117 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 492 2346 2418
Size: 492 1854 72
Fragments arranged by size:
1854 492 72
BsrBI CCG'CTC
Cuts at: 0 294 741 882 932 1164 2159 2418
Size: 294 447 141 50 232 995 259
Fragments arranged by size:
995 447 294 259 232 141 50
BsrDI GCAATG_nn'
Cuts at: 0 1656 2415 2418
Size: 1656 759 3
Fragments arranged by size:
1656 759 3
BsrFI r'CCGG_y
Cuts at: 0 564 1007 1632 2147 2151 2418
Size: 564 443 625 515 4 267
Fragments arranged by size:
625 564 515 443 267 4
BsrGI T'GTAC_A
Cuts at: 0 440 2418
Size: 440 1978
BssHII G'CGCG_C
Cuts at: 0 655 657 2418
Size: 655 2 1761
Fragments arranged by size:
1761 655 2
BssSI C'ACGA_G
Cuts at: 0 268 1759 2418
Size: 268 1491 659
Fragments arranged by size:
1491 659 268
BstAPI GCAn_nnn'nTGC
Cuts at: 0 950 2276 2418
Size: 950 1326 142
Fragments arranged by size:
1326 950 142
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1146 1378 2418
Size: 1146 232 1040
Fragments arranged by size:
1146 1040 232
BstYI r'GATC_y
Cuts at: 0 44 131 764 1307 1491 2287 2418
Size: 44 87 633 543 184 796 131
Fragments arranged by size:
796 633 543 184 131 87 44
BtgI C'CryG_G
Cuts at: 0 59 366 902 1313 1452 1514 2418
Size: 59 307 536 411 139 62 904
Fragments arranged by size:
904 536 411 307 139 62 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1052 2418
Size: 1052 1366
BtsI GCAGTG_nn'
Cuts at: 0 948 1095 2418
Size: 948 147 1323
Fragments arranged by size:
1323 948 147
DraIII CAC_nnn'GTG
Cuts at: 0 41 84 1325 2265 2418
Size: 41 43 1241 940 153
Fragments arranged by size:
1241 940 153 43 41
DrdI GACnn_nn'nnGTC
Cuts at: 0 223 2418
Size: 223 2195
EaeI y'GGCC_r
Cuts at: 0 201 377 883 1202 1389 1883 2149 2412
Size: 201 176 506 319 187 494 266 263
Cuts at: 2412 2418
Size: 6
Fragments arranged by size:
506 494 319 266 263 201 187 176
6
EagI C'GGCC_G
Cuts at: 0 377 883 1389 2149 2418
Size: 377 506 506 760 269
Fragments arranged by size:
760 506 506 377 269
EarI CTCTTCn'nnn_
Cuts at: 0 217 922 2398 2418
Size: 217 705 1476 20
Fragments arranged by size:
1476 705 217 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 141 217 247 2418
Size: 141 76 30 2171
Fragments arranged by size:
2171 141 76 30
EcoICRI GAG'CTC
Cuts at: 0 306 2418
Size: 306 2112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 624 709 753 2418
Size: 592 32 85 44 1665
Fragments arranged by size:
1665 592 85 44 32
EcoO109I rG'GnC_Cy
Cuts at: 0 525 684 1014 2018 2127 2418
Size: 525 159 330 1004 109 291
Fragments arranged by size:
1004 525 330 291 159 109
FspI TGC'GCA
Cuts at: 0 1648 2418
Size: 1648 770
HaeII r_GCGC'y
Cuts at: 0 571 1561 1770 2418
Size: 571 990 209 648
Fragments arranged by size:
990 648 571 209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 79 111 304 336 343 375 1328 1360
Size: 79 32 193 32 7 32 953 32
Cuts at: 1360 1606 1638 2418
Size: 246 32 780
Fragments arranged by size:
953 780 246 193 79 32 32 32
32 32 7
HincII GTy'rAC
Cuts at: 0 909 2418
Size: 909 1509
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 335 826 1307 2418
Size: 56 279 491 481 1111
Fragments arranged by size:
1111 491 481 279 56
MscI TGG'CCA
Cuts at: 0 1885 2418
Size: 1885 533
MslI CAynn'nnrTG
Cuts at: 0 478 856 2248 2356 2418
Size: 478 378 1392 108 62
Fragments arranged by size:
1392 478 378 108 62
MspA1I CmG'CkG
Cuts at: 0 1221 1516 1687 2312 2418
Size: 1221 295 171 625 106
Fragments arranged by size:
1221 625 295 171 106
NaeI GCC'GGC
Cuts at: 0 566 1009 2153 2418
Size: 566 443 1144 265
Fragments arranged by size:
1144 566 443 265
NcoI C'CATG_G
Cuts at: 0 59 902 1452 2418
Size: 59 843 550 966
Fragments arranged by size:
966 843 550 59
NgoMIV G'CCGG_C
Cuts at: 0 564 1007 2151 2418
Size: 564 443 1144 267
Fragments arranged by size:
1144 564 443 267
NotI GC'GGCC_GC
Cuts at: 0 377 2418
Size: 377 2041
NspI r_CATG'y
Cuts at: 0 417 2418
Size: 417 2001
PciI A'CATG_T
Cuts at: 0 413 2418
Size: 413 2005
PflMI CCAn_nnn'nTGG
Cuts at: 0 84 252 1616 2117 2418
Size: 84 168 1364 501 301
Fragments arranged by size:
1364 501 301 168 84
PfoI T'CCnGG_A
Cuts at: 0 134 769 2418
Size: 134 635 1649
Fragments arranged by size:
1649 635 134
PmlI CAC'GTG
Cuts at: 0 150 594 1374 2418
Size: 150 444 780 1044
Fragments arranged by size:
1044 780 444 150
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2185 2215 2217 2247 2418
Size: 2185 30 2 30 171
Fragments arranged by size:
2185 171 30 30 2
PpuMI rG'GwC_Cy
Cuts at: 0 684 1014 2018 2127 2418
Size: 684 330 1004 109 291
Fragments arranged by size:
1004 684 330 291 109
PstI C_TGCA'G
Cuts at: 0 167 550 2375 2418
Size: 167 383 1825 43
Fragments arranged by size:
1825 383 167 43
PvuII CAG'CTG
Cuts at: 0 2312 2418
Size: 2312 106
SacI G_AGCT'C
Cuts at: 0 308 2418
Size: 308 2110
SacII CC_GC'GG
Cuts at: 0 1517 2418
Size: 1517 901
SanDI GG'GwC_CC
Cuts at: 0 2018 2418
Size: 2018 400
SapI GCTCTTCn'nnn_
Cuts at: 0 922 2398 2418
Size: 922 1476 20
Fragments arranged by size:
1476 922 20
SfcI C'TryA_G
Cuts at: 0 163 177 546 2058 2371 2418
Size: 163 14 369 1512 313 47
Fragments arranged by size:
1512 369 313 163 47 14
SmaI CCC'GGG
Cuts at: 0 535 1304 2418
Size: 535 769 1114
Fragments arranged by size:
1114 769 535
SmlI C'TyrA_G
Cuts at: 0 2140 2418
Size: 2140 278
StuI AGG'CCT
Cuts at: 0 161 2418
Size: 161 2257
StyI C'CwwG_G
Cuts at: 0 59 107 287 734 902 1259 1452 1610
Size: 59 48 180 447 168 357 193 158
Cuts at: 1610 1804 2045 2418
Size: 194 241 373
Fragments arranged by size:
447 373 357 241 194 193 180 168
158 59 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 379 2418
Size: 379 2039
TatI w'GTAC_w
Cuts at: 0 440 2418
Size: 440 1978
Tth111I GACn'n_nGTC
Cuts at: 0 865 2418
Size: 865 1553
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 249 567 816 1557 2418
Size: 249 318 249 741 861
Fragments arranged by size:
861 741 318 249 249
XmaI C'CCGG_G
Cuts at: 0 533 1302 2418
Size: 533 769 1116
Fragments arranged by size:
1116 769 533
XmnI GAAnn'nnTTC
Cuts at: 0 1462 2418
Size: 1462 956
ZraI GAC'GTC
Cuts at: 0 582 648 2418
Size: 582 66 1770
Fragments arranged by size:
1770 582 66
Enzymes that do cut:
AarI AatII AflIII AhdI AleI AloI AlwNI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BciVI BclI
BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I FspI HaeII Hin4I HincII MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NotI NspI PciI PflMI
PfoI PmlI PpiI PpuMI PstI PvuII SacI SacII
SanDI SapI SfcI SmaI SmlI StuI StyI TaqII
TatI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AgeI AlfI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BcgI BfrBI BglI BlpI BmtI BsaBI BsgI BsiWI
BsmI BspEI BspHI BstBI BstEII BstZ17I Bsu36I ClaI
CspCI DraI EcoNI EcoRI EcoRV FalI FseI FspAI
HindIII HpaI KasI KpnI MfeI MluI NarI NdeI
NheI NruI NsiI PacI PasI PmeI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TstI XbaI XhoI