Restriction map for
pFN21AB6288
(Linear) (Six-base) MAPSORT of: KIBB6288.seq Check: 8650 from: 1 to: 2352
KIBB6288.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1101 1173 1621 2352
Size: 1101 72 448 731
Fragments arranged by size:
1101 731 448 72
AclI AA'CG_TT
Cuts at: 0 347 2352
Size: 347 2005
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 1117 1130 1162 1373 1582 2352
Size: 496 621 13 32 211 209 770
Fragments arranged by size:
770 621 496 211 209 32 13
AflIII A'CryG_T
Cuts at: 0 2172 2352
Size: 2172 180
AleI CACnn'nnGTG
Cuts at: 0 1012 1492 1798 2352
Size: 1012 480 306 554
Fragments arranged by size:
1012 554 480 306
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 177 329 2352
Size: 177 152 2023
Fragments arranged by size:
2023 177 152
AlwNI CAG_nnn'CTG
Cuts at: 0 646 1030 2352
Size: 646 384 1322
Fragments arranged by size:
1322 646 384
ApoI r'AATT_y
Cuts at: 0 533 584 1170 1443 2347 2352
Size: 533 51 586 273 904 5
Fragments arranged by size:
904 586 533 273 51 5
AvaI C'yCGr_G
Cuts at: 0 2229 2352
Size: 2229 123
BanI G'GyrC_C
Cuts at: 0 1549 2352
Size: 1549 803
BanII G_rGCy'C
Cuts at: 0 1021 1123 1374 2081 2352
Size: 1021 102 251 707 271
Fragments arranged by size:
1021 707 271 251 102
BbsI GAAGACnn'nnnn_
Cuts at: 0 1490 2352
Size: 1490 862
BbvCI CC'TCA_GC
Cuts at: 0 1235 2352
Size: 1235 1117
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1341 1375 2352
Size: 1341 34 977
Fragments arranged by size:
1341 977 34
BclI T'GATC_A
Cuts at: 0 1830 2157 2352
Size: 1830 327 195
Fragments arranged by size:
1830 327 195
BlpI GC'TnA_GC
Cuts at: 0 1020 2352
Size: 1020 1332
Bme1580I G_kGCm'C
Cuts at: 0 1554 2352
Size: 1554 798
BmrI ACTGGGnnnn_n'
Cuts at: 0 848 1486 2352
Size: 848 638 866
Fragments arranged by size:
866 848 638
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 422 2352
Size: 422 1930
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1107 1264 2036 2065 2352
Size: 1107 157 772 29 287
Fragments arranged by size:
1107 772 287 157 29
Bpu10I CC'TnA_GC
Cuts at: 0 1235 2352
Size: 1235 1117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 2285 2352
Size: 737 1548 67
Fragments arranged by size:
1548 737 67
BsaBI GATnn'nnATC
Cuts at: 0 1222 2309 2352
Size: 1222 1087 43
Fragments arranged by size:
1222 1087 43
BsaWI w'CCGG_w
Cuts at: 0 687 2352
Size: 687 1665
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1640 1670 1691 1710 1721 1740 2352
Size: 1640 30 21 19 11 19 612
Fragments arranged by size:
1640 612 30 21 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 37 40 43 128 314 800 845
Size: 34 3 3 3 85 186 486 45
Cuts at: 845 945 948 2352
Size: 100 3 1404
Fragments arranged by size:
1404 486 186 100 85 45 34 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 1462 1574 2352
Size: 1462 112 778
Fragments arranged by size:
1462 778 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 954 1663 2352
Size: 954 709 689
Fragments arranged by size:
954 709 689
BsiHKAI G_wGCw'C
Cuts at: 0 1021 1123 1998 2081 2352
Size: 1021 102 875 83 271
Fragments arranged by size:
1021 875 271 102 83
BsmI GAATG_Cn'
Cuts at: 0 1151 1158 1464 2128 2352
Size: 1151 7 306 664 224
Fragments arranged by size:
1151 664 306 224 7
Bsp1286I G_dGCh'C
Cuts at: 0 1021 1123 1374 1554 1998 2081 2352
Size: 1021 102 251 180 444 83 271
Fragments arranged by size:
1021 444 271 251 180 102 83
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 592 834 976 1101 1173 1621 1666 2352
Size: 592 242 142 125 72 448 45 686
Fragments arranged by size:
686 592 448 242 142 125 72 45
BsrGI T'GTAC_A
Cuts at: 0 486 1413 2352
Size: 486 927 939
Fragments arranged by size:
939 927 486
BstBI TT'CG_AA
Cuts at: 0 1860 2308 2352
Size: 1860 448 44
Fragments arranged by size:
1860 448 44
BstYI r'GATC_y
Cuts at: 0 222 1217 1223 2352
Size: 222 995 6 1129
Fragments arranged by size:
1129 995 222 6
Bsu36I CC'TnA_GG
Cuts at: 0 111 2352
Size: 111 2241
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 64 2352
Size: 64 2288
BtsI GCAGTG_nn'
Cuts at: 0 718 977 1008 2352
Size: 718 259 31 1344
Fragments arranged by size:
1344 718 259 31
DraIII CAC_nnn'GTG
Cuts at: 0 2071 2352
Size: 2071 281
EaeI y'GGCC_r
Cuts at: 0 849 1032 2352
Size: 849 183 1320
Fragments arranged by size:
1320 849 183
EarI CTCTTCn'nnn_
Cuts at: 0 740 1181 1857 1899 2352
Size: 740 441 676 42 453
Fragments arranged by size:
740 676 453 441 42
EcoICRI GAG'CTC
Cuts at: 0 1019 1121 2079 2352
Size: 1019 102 958 273
Fragments arranged by size:
1019 958 273 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 1107 1117 1130 1162 1264 1373 1582
Size: 496 611 10 13 32 102 109 209
Cuts at: 1582 2036 2065 2352
Size: 454 29 287
Fragments arranged by size:
611 496 454 287 209 109 102 32
29 13 10
EcoRI G'AATT_C
Cuts at: 0 2347 2352
Size: 2347 5
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1110 1142 1691 1723 1815 1847 2145 2149
Size: 1110 32 549 32 92 32 298 4
Cuts at: 2149 2177 2181 2352
Size: 28 4 171
Fragments arranged by size:
1110 549 298 171 92 32 32 32
28 4 4
HincII GTy'rAC
Cuts at: 0 529 2352
Size: 529 1823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 1251 1375 1399 1725 2352
Size: 571 680 124 24 326 627
Fragments arranged by size:
680 627 571 326 124 24
MscI TGG'CCA
Cuts at: 0 851 1034 2352
Size: 851 183 1318
Fragments arranged by size:
1318 851 183
MslI CAynn'nnrTG
Cuts at: 0 1012 1492 1798 2105 2352
Size: 1012 480 306 307 247
Fragments arranged by size:
1012 480 307 306 247
MspA1I CmG'CkG
Cuts at: 0 40 46 628 2352
Size: 40 6 582 1724
Fragments arranged by size:
1724 582 40 6
NspI r_CATG'y
Cuts at: 0 2176 2352
Size: 2176 176
PciI A'CATG_T
Cuts at: 0 2172 2352
Size: 2172 180
PstI C_TGCA'G
Cuts at: 0 603 725 1632 2352
Size: 603 122 907 720
Fragments arranged by size:
907 720 603 122
PvuII CAG'CTG
Cuts at: 0 628 2352
Size: 628 1724
SacI G_AGCT'C
Cuts at: 0 1021 1123 2081 2352
Size: 1021 102 958 271
Fragments arranged by size:
1021 958 271 102
ScaI AGT'ACT
Cuts at: 0 936 2352
Size: 936 1416
SexAI A'CCwGG_T
Cuts at: 0 702 2352
Size: 702 1650
SfcI C'TryA_G
Cuts at: 0 56 565 599 721 1628 1721 1736 2352
Size: 56 509 34 122 907 93 15 616
Fragments arranged by size:
907 616 509 122 93 56 34 15
SmlI C'TyrA_G
Cuts at: 0 752 2300 2352
Size: 752 1548 52
Fragments arranged by size:
1548 752 52
SpeI A'CTAG_T
Cuts at: 0 550 1666 2352
Size: 550 1116 686
Fragments arranged by size:
1116 686 550
SspI AAT'ATT
Cuts at: 0 654 1428 2352
Size: 654 774 924
Fragments arranged by size:
924 774 654
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 924 1612 2352
Size: 924 688 740
Fragments arranged by size:
924 740 688
TatI w'GTAC_w
Cuts at: 0 465 486 934 1413 2352
Size: 465 21 448 479 939
Fragments arranged by size:
939 479 465 448 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 168 200 2034 2066 2352
Size: 168 32 1834 32 286
Fragments arranged by size:
1834 286 168 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 700 2352
Size: 700 1652
Enzymes that do cut:
AarI AclI AcuI AflIII AleI AlfI AlwNI ApoI
AvaI BanI BanII BbsI BbvCI BcgI BclI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrGI BstBI BstYI Bsu36I BtgZI BtsI DraIII EaeI
EarI EcoICRI Eco57MI EcoRI Hin4I HincII MmeI MscI
MslI MspA1I NspI PciI PstI PvuII SacI ScaI
SexAI SfcI SmlI SpeI SspI TaqII TatI TstI
XcmI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BciVI BfrBI BglI BglII BmgBI BmtI BsaI
BsaAI BsaHI BsiEI BsiWI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstZ17I
BtgI ClaI CspCI DraI DrdI EagI EciI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI StuI StyI SwaI Tth111I XbaI XhoI
XmaI XmnI ZraI