Restriction map for
pFN21AB6282
(Linear) (Six-base) MAPSORT of: KIBB6282.seq Check: 8610 from: 1 to: 2343
KIBB6282.orf
With 182 enzymes: SgfI *
March 22, 2012 15:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 147 2343
Size: 147 2196
AccI GT'mk_AC
Cuts at: 0 2115 2343
Size: 2115 228
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1289 2343
Size: 1289 1054
AflII C'TTAA_G
Cuts at: 0 1534 2343
Size: 1534 809
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 92 124 2343
Size: 92 32 2219
Fragments arranged by size:
2219 92 32
ApaI G_GGCC'C
Cuts at: 0 190 456 2343
Size: 190 266 1887
Fragments arranged by size:
1887 266 190
ApoI r'AATT_y
Cuts at: 0 766 798 961 980 993 1417 1588 1699
Size: 766 32 163 19 13 424 171 111
Cuts at: 1699 2136 2221 2343
Size: 437 85 122
Fragments arranged by size:
766 437 424 171 163 122 111 85
32 19 13
AvaI C'yCGr_G
Cuts at: 0 111 2343
Size: 111 2232
BamHI G'GATC_C
Cuts at: 0 1710 2343
Size: 1710 633
BanI G'GyrC_C
Cuts at: 0 61 90 2343
Size: 61 29 2253
Fragments arranged by size:
2253 61 29
BanII G_rGCy'C
Cuts at: 0 190 220 456 2343
Size: 190 30 236 1887
Fragments arranged by size:
1887 236 190 30
BbeI G_GCGC'C
Cuts at: 0 65 94 2343
Size: 65 29 2249
Fragments arranged by size:
2249 65 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 338 1242 2343
Size: 338 904 1101
Fragments arranged by size:
1101 904 338
BbvCI CC'TCA_GC
Cuts at: 0 608 2343
Size: 608 1735
BciVI GTATCCnnnnn_n'
Cuts at: 0 1889 2343
Size: 1889 454
BclI T'GATC_A
Cuts at: 0 333 2343
Size: 333 2010
BfrBI ATG'CAT
Cuts at: 0 1053 1478 2343
Size: 1053 425 865
Fragments arranged by size:
1053 865 425
BglI GCCn_nnn'nGGC
Cuts at: 0 194 2343
Size: 194 2149
BglII A'GATC_T
Cuts at: 0 729 2343
Size: 729 1614
BlpI GC'TnA_GC
Cuts at: 0 80 2343
Size: 80 2263
Bme1580I G_kGCm'C
Cuts at: 0 190 456 2343
Size: 190 266 1887
Fragments arranged by size:
1887 266 190
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 153 2343
Size: 153 2190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 2343
Size: 161 2182
Bpu10I CC'TnA_GC
Cuts at: 0 608 2343
Size: 608 1735
BsaAI yAC'GTr
Cuts at: 0 888 2343
Size: 888 1455
BsaBI GATnn'nnATC
Cuts at: 0 1526 2343
Size: 1526 817
BsaHI Gr'CG_yC
Cuts at: 0 62 91 2343
Size: 62 29 2252
Fragments arranged by size:
2252 62 29
BsaWI w'CCGG_w
Cuts at: 0 105 176 2343
Size: 105 71 2167
Fragments arranged by size:
2167 105 71
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 82 158 306 483 2343
Size: 82 76 148 177 1860
Fragments arranged by size:
1860 177 148 82 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2183 2343
Size: 2183 160
BsiHKAI G_wGCw'C
Cuts at: 0 220 2343
Size: 220 2123
Bsp1286I G_dGCh'C
Cuts at: 0 190 220 456 2343
Size: 190 30 236 1887
Fragments arranged by size:
1887 236 190 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 147 2343
Size: 147 2196
BsrBI CCG'CTC
Cuts at: 0 860 2343
Size: 860 1483
BsrDI GCAATG_nn'
Cuts at: 0 1432 1749 2343
Size: 1432 317 594
Fragments arranged by size:
1432 594 317
BsrGI T'GTAC_A
Cuts at: 0 480 2343
Size: 480 1863
BssHII G'CGCG_C
Cuts at: 0 33 152 2343
Size: 33 119 2191
Fragments arranged by size:
2191 119 33
BssSI C'ACGA_G
Cuts at: 0 191 2343
Size: 191 2152
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2126 2343
Size: 2126 217
BstYI r'GATC_y
Cuts at: 0 398 729 748 1710 2343
Size: 398 331 19 962 633
Fragments arranged by size:
962 633 398 331 19
Bsu36I CC'TnA_GG
Cuts at: 0 868 2343
Size: 868 1475
BtgI C'CryG_G
Cuts at: 0 1567 2343
Size: 1567 776
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 71 2343
Size: 71 2272
BtsI GCAGTG_nn'
Cuts at: 0 750 1608 2343
Size: 750 858 735
Fragments arranged by size:
858 750 735
DraI TTT'AAA
Cuts at: 0 1067 1493 1556 2343
Size: 1067 426 63 787
Fragments arranged by size:
1067 787 426 63
EaeI y'GGCC_r
Cuts at: 0 195 446 2343
Size: 195 251 1897
Fragments arranged by size:
1897 251 195
EarI CTCTTCn'nnn_
Cuts at: 0 122 290 505 1202 1963 2343
Size: 122 168 215 697 761 380
Fragments arranged by size:
761 697 380 215 168 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 779 2343
Size: 779 1564
EcoICRI GAG'CTC
Cuts at: 0 218 2343
Size: 218 2125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 1289 2343
Size: 161 1128 1054
Fragments arranged by size:
1128 1054 161
EcoO109I rG'GnC_Cy
Cuts at: 0 186 187 452 2343
Size: 186 1 265 1891
Fragments arranged by size:
1891 265 186 1
EcoRI G'AATT_C
Cuts at: 0 1699 2221 2343
Size: 1699 522 122
Fragments arranged by size:
1699 522 122
HaeII r_GCGC'y
Cuts at: 0 65 94 2343
Size: 65 29 2249
Fragments arranged by size:
2249 65 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 244 276 922 954 1141 1173 1201 1203
Size: 244 32 646 32 187 32 28 2
Cuts at: 1203 1233 1235 1702 1734 2343
Size: 30 2 467 32 609
Fragments arranged by size:
646 609 467 244 187 32 32 32
32 30 28 2 2
HincII GTy'rAC
Cuts at: 0 1721 2343
Size: 1721 622
HindIII A'AGCT_T
Cuts at: 0 1460 1486 2270 2343
Size: 1460 26 784 73
Fragments arranged by size:
1460 784 73 26
KasI G'GCGC_C
Cuts at: 0 61 90 2343
Size: 61 29 2253
Fragments arranged by size:
2253 61 29
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1520 2343
Size: 1520 823
MscI TGG'CCA
Cuts at: 0 448 2343
Size: 448 1895
NarI GG'CG_CC
Cuts at: 0 62 91 2343
Size: 62 29 2252
Fragments arranged by size:
2252 62 29
NcoI C'CATG_G
Cuts at: 0 1567 2343
Size: 1567 776
NsiI A_TGCA'T
Cuts at: 0 1055 1480 2343
Size: 1055 425 863
Fragments arranged by size:
1055 863 425
NspI r_CATG'y
Cuts at: 0 1057 2114 2343
Size: 1057 1057 229
Fragments arranged by size:
1057 1057 229
PsiI TTA'TAA
Cuts at: 0 811 2343
Size: 811 1532
PspOMI G'GGCC_C
Cuts at: 0 186 452 2343
Size: 186 266 1891
Fragments arranged by size:
1891 266 186
SacI G_AGCT'C
Cuts at: 0 220 2343
Size: 220 2123
SapI GCTCTTCn'nnn_
Cuts at: 0 122 1963 2343
Size: 122 1841 380
Fragments arranged by size:
1841 380 122
SexAI A'CCwGG_T
Cuts at: 0 2099 2343
Size: 2099 244
SfcI C'TryA_G
Cuts at: 0 844 1580 1892 2343
Size: 844 736 312 451
Fragments arranged by size:
844 736 451 312
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 194 2343
Size: 194 2149
SfoI GGC'GCC
Cuts at: 0 63 92 2343
Size: 63 29 2251
Fragments arranged by size:
2251 63 29
SmaI CCC'GGG
Cuts at: 0 113 2343
Size: 113 2230
SmlI C'TyrA_G
Cuts at: 0 1534 2343
Size: 1534 809
SnaBI TAC'GTA
Cuts at: 0 888 2343
Size: 888 1455
SphI G_CATG'C
Cuts at: 0 1057 2343
Size: 1057 1286
SspI AAT'ATT
Cuts at: 0 1407 1968 2343
Size: 1407 561 375
Fragments arranged by size:
1407 561 375
StuI AGG'CCT
Cuts at: 0 1949 2343
Size: 1949 394
StyI C'CwwG_G
Cuts at: 0 1335 1567 2343
Size: 1335 232 776
Fragments arranged by size:
1335 776 232
TatI w'GTAC_w
Cuts at: 0 480 2343
Size: 480 1863
XbaI T'CTAG_A
Cuts at: 0 2212 2343
Size: 2212 131
XmaI C'CCGG_G
Cuts at: 0 111 2343
Size: 111 2232
XmnI GAAnn'nnTTC
Cuts at: 0 1439 2246 2343
Size: 1439 807 97
Fragments arranged by size:
1439 807 97
Enzymes that do cut:
AarI AccI AcuI AflII AloI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BplI BpmI Bpu10I
BsaAI BsaBI BsaHI BsaWI BseRI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrDI BsrGI BssHII BssSI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII HindIII
KasI MmeI MscI NarI NcoI NsiI NspI PsiI
PspOMI SacI SapI SexAI SfcI SfiI SfoI SmaI
SmlI SnaBI SphI SspI StuI StyI TatI XbaI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI
AlfI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI
BcgI BmgBI BmrI BmtI BpuEI BsaI BsaXI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrFI BstAPI
BstBI BstEII BstZ17I ClaI CspCI DraIII DrdI EagI
EcoNI EcoRV FalI FseI FspI FspAI HpaI KpnI
MfeI MluI MslI MspA1I NaeI NdeI NgoMIV NheI
NotI NruI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SgfI
SgrAI SpeI SrfI SwaI TaqII TstI Tth111I XcmI
XhoI ZraI