Restriction map for
pFN21AB6270
(Linear) (Six-base) MAPSORT of: KIBB6270.seq Check: 6884 from: 1 to: 2316
KIBB6270.orf
With 182 enzymes: SgfI *
December 27, 2011 16:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2197 2316
Size: 2197 119
AfeI AGC'GCT
Cuts at: 0 420 2316
Size: 420 1896
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2020 2316
Size: 2020 296
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 922 954 2316
Size: 922 32 1362
Fragments arranged by size:
1362 922 32
AlwNI CAG_nnn'CTG
Cuts at: 0 957 1255 2082 2316
Size: 957 298 827 234
Fragments arranged by size:
957 827 298 234
ApaI G_GGCC'C
Cuts at: 0 195 220 2316
Size: 195 25 2096
Fragments arranged by size:
2096 195 25
ApaLI G'TGCA_C
Cuts at: 0 1129 1921 2316
Size: 1129 792 395
Fragments arranged by size:
1129 792 395
AvaI C'yCGr_G
Cuts at: 0 148 2316
Size: 148 2168
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2132 2165 2316
Size: 2132 33 151
Fragments arranged by size:
2132 151 33
BanI G'GyrC_C
Cuts at: 0 22 1205 2316
Size: 22 1183 1111
Fragments arranged by size:
1183 1111 22
BanII G_rGCy'C
Cuts at: 0 195 220 431 475 975 1568 2316
Size: 195 25 211 44 500 593 748
Fragments arranged by size:
748 593 500 211 195 44 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 898 2055 2164 2316
Size: 898 1157 109 152
Fragments arranged by size:
1157 898 152 109
BbvCI CC'TCA_GC
Cuts at: 0 365 782 1791 2316
Size: 365 417 1009 525
Fragments arranged by size:
1009 525 417 365
BglI GCCn_nnn'nGGC
Cuts at: 0 1727 1847 1951 2316
Size: 1727 120 104 365
Fragments arranged by size:
1727 365 120 104
BglII A'GATC_T
Cuts at: 0 336 2316
Size: 336 1980
Bme1580I G_kGCm'C
Cuts at: 0 195 220 1133 1663 1925 2316
Size: 195 25 913 530 262 391
Fragments arranged by size:
913 530 391 262 195 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 727 1152 1189 1301 2316
Size: 727 425 37 112 1015
Fragments arranged by size:
1015 727 425 112 37
BmtI G_CTAG'C
Cuts at: 0 554 2316
Size: 554 1762
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 420 1587 2316
Size: 420 1167 729
Fragments arranged by size:
1167 729 420
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 236 644 966 1072 1384 1476 2254
Size: 70 166 408 322 106 312 92 778
Cuts at: 2254 2316
Size: 62
Fragments arranged by size:
778 408 322 312 166 106 92 70
62
Bpu10I CC'TnA_GC
Cuts at: 0 365 782 974 1791 2316
Size: 365 417 192 817 525
Fragments arranged by size:
817 525 417 365 192
BsaBI GATnn'nnATC
Cuts at: 0 263 1438 2316
Size: 263 1175 878
Fragments arranged by size:
1175 878 263
BsaHI Gr'CG_yC
Cuts at: 0 1860 2316
Size: 1860 456
BsaWI w'CCGG_w
Cuts at: 0 92 1652 2316
Size: 92 1560 664
Fragments arranged by size:
1560 664 92
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 922 952 2316
Size: 922 30 1364
Fragments arranged by size:
1364 922 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 393 421 496 499 583 652 946 949
Size: 393 28 75 3 84 69 294 3
Cuts at: 949 1538 2245 2316
Size: 589 707 71
Fragments arranged by size:
707 589 393 294 84 75 71 69
28 3 3
BseYI C'CCAG_C
Cuts at: 0 1174 1386 1567 1982 2180 2316
Size: 1174 212 181 415 198 136
Fragments arranged by size:
1174 415 212 198 181 136
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 2316
Size: 793 1523
BsiEI CG_ry'CG
Cuts at: 0 1851 2316
Size: 1851 465
BsiHKAI G_wGCw'C
Cuts at: 0 431 975 1133 1522 1925 2203 2316
Size: 431 544 158 389 403 278 113
Fragments arranged by size:
544 431 403 389 278 158 113
BsiWI C'GTAC_G
Cuts at: 0 51 2316
Size: 51 2265
BsmI GAATG_Cn'
Cuts at: 0 616 2316
Size: 616 1700
Bsp1286I G_dGCh'C
Cuts at: 0 195 220 431 475 975 1133 1522 1568
Size: 195 25 211 44 500 158 389 46
Cuts at: 1568 1663 1925 2203 2316
Size: 95 262 278 113
Fragments arranged by size:
500 389 278 262 211 195 158 113
95 46 44 25
BspEI T'CCGG_A
Cuts at: 0 92 2316
Size: 92 2224
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 965 2316
Size: 965 1351
BsrBI CCG'CTC
Cuts at: 0 381 1967 2316
Size: 381 1586 349
Fragments arranged by size:
1586 381 349
BsrDI GCAATG_nn'
Cuts at: 0 1716 2316
Size: 1716 600
BsrFI r'CCGG_y
Cuts at: 0 1510 1757 2316
Size: 1510 247 559
Fragments arranged by size:
1510 559 247
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1202 1450 2152 2316
Size: 1202 248 702 164
Fragments arranged by size:
1202 702 248 164
BstYI r'GATC_y
Cuts at: 0 258 336 1439 2316
Size: 258 78 1103 877
Fragments arranged by size:
1103 877 258 78
Bsu36I CC'TnA_GG
Cuts at: 0 405 2316
Size: 405 1911
BtgI C'CryG_G
Cuts at: 0 18 326 503 776 2316
Size: 18 308 177 273 1540
Fragments arranged by size:
1540 308 273 177 18
BtsI GCAGTG_nn'
Cuts at: 0 545 1992 2316
Size: 545 1447 324
Fragments arranged by size:
1447 545 324
ClaI AT'CG_AT
Cuts at: 0 2054 2316
Size: 2054 262
DraIII CAC_nnn'GTG
Cuts at: 0 1197 2316
Size: 1197 1119
DrdI GACnn_nn'nnGTC
Cuts at: 0 844 2316
Size: 844 1472
EaeI y'GGCC_r
Cuts at: 0 1091 1403 1686 1848 2316
Size: 1091 312 283 162 468
Fragments arranged by size:
1091 468 312 283 162
EagI C'GGCC_G
Cuts at: 0 1848 2316
Size: 1848 468
EarI CTCTTCn'nnn_
Cuts at: 0 3 442 676 1939 2116 2316
Size: 3 439 234 1263 177 200
Fragments arranged by size:
1263 439 234 200 177 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 244 2316
Size: 244 2072
EcoICRI GAG'CTC
Cuts at: 0 429 973 2316
Size: 429 544 1343
Fragments arranged by size:
1343 544 429
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 236 644 966 1072 1384 1476 2197
Size: 70 166 408 322 106 312 92 721
Cuts at: 2197 2254 2316
Size: 57 62
Fragments arranged by size:
721 408 322 312 166 106 92 70
62 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 593 673 917 2316
Size: 593 80 244 1399
Fragments arranged by size:
1399 593 244 80
EcoO109I rG'GnC_Cy
Cuts at: 0 46 191 192 217 270 528 1295 1628
Size: 46 145 1 25 53 258 767 333
Cuts at: 1628 2190 2316
Size: 562 126
Fragments arranged by size:
767 562 333 258 145 126 53 46
25 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 726 1461 2316
Size: 726 735 855
Fragments arranged by size:
855 735 726
FspI TGC'GCA
Cuts at: 0 961 2316
Size: 961 1355
HaeII r_GCGC'y
Cuts at: 0 422 2316
Size: 422 1894
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 49 81 472 504 999 1031 1518 1550
Size: 49 32 391 32 495 32 487 32
Cuts at: 1550 2316
Size: 766
Fragments arranged by size:
766 495 487 391 49 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1062 2316
Size: 1062 1254
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 710 2316
Size: 470 240 1606
Fragments arranged by size:
1606 470 240
MscI TGG'CCA
Cuts at: 0 1093 1405 1688 2316
Size: 1093 312 283 628
Fragments arranged by size:
1093 628 312 283
MslI CAynn'nnrTG
Cuts at: 0 815 1448 1466 2316
Size: 815 633 18 850
Fragments arranged by size:
850 815 633 18
MspA1I CmG'CkG
Cuts at: 0 114 328 369 944 2316
Size: 114 214 41 575 1372
Fragments arranged by size:
1372 575 214 114 41
NaeI GCC'GGC
Cuts at: 0 1512 1759 2316
Size: 1512 247 557
Fragments arranged by size:
1512 557 247
NcoI C'CATG_G
Cuts at: 0 18 503 2316
Size: 18 485 1813
Fragments arranged by size:
1813 485 18
NgoMIV G'CCGG_C
Cuts at: 0 1510 1757 2316
Size: 1510 247 559
Fragments arranged by size:
1510 559 247
NheI G'CTAG_C
Cuts at: 0 550 2316
Size: 550 1766
PasI CC'CwG_GG
Cuts at: 0 1026 1582 2316
Size: 1026 556 734
Fragments arranged by size:
1026 734 556
PflMI CCAn_nnn'nTGG
Cuts at: 0 340 1197 2316
Size: 340 857 1119
Fragments arranged by size:
1119 857 340
PfoI T'CCnGG_A
Cuts at: 0 374 674 2231 2316
Size: 374 300 1557 85
Fragments arranged by size:
1557 374 300 85
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 922 954 2316
Size: 922 32 1362
Fragments arranged by size:
1362 922 32
PpuMI rG'GwC_Cy
Cuts at: 0 46 270 528 1628 2190 2316
Size: 46 224 258 1100 562 126
Fragments arranged by size:
1100 562 258 224 126 46
PshAI GACnn'nnGTC
Cuts at: 0 2164 2242 2316
Size: 2164 78 74
Fragments arranged by size:
2164 78 74
PspOMI G'GGCC_C
Cuts at: 0 191 216 2316
Size: 191 25 2100
Fragments arranged by size:
2100 191 25
PstI C_TGCA'G
Cuts at: 0 911 1544 2316
Size: 911 633 772
Fragments arranged by size:
911 772 633
PvuII CAG'CTG
Cuts at: 0 369 944 2316
Size: 369 575 1372
Fragments arranged by size:
1372 575 369
SacI G_AGCT'C
Cuts at: 0 431 975 2316
Size: 431 544 1341
Fragments arranged by size:
1341 544 431
SacII CC_GC'GG
Cuts at: 0 329 2316
Size: 329 1987
SfcI C'TryA_G
Cuts at: 0 907 1540 1605 2316
Size: 907 633 65 711
Fragments arranged by size:
907 711 633 65
StuI AGG'CCT
Cuts at: 0 1789 2316
Size: 1789 527
StyI C'CwwG_G
Cuts at: 0 18 503 1825 2316
Size: 18 485 1322 491
Fragments arranged by size:
1322 491 485 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 187 1117 1208 1508 2316
Size: 187 930 91 300 808
Fragments arranged by size:
930 808 300 187 91
TatI w'GTAC_w
Cuts at: 0 761 2316
Size: 761 1555
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2182 2214 2316
Size: 2182 32 102
Fragments arranged by size:
2182 102 32
XmnI GAAnn'nnTTC
Cuts at: 0 709 2316
Size: 709 1607
Enzymes that do cut:
AcuI AfeI AlfI AloI AlwNI ApaI ApaLI AvaI
BaeI BanI BanII BbsI BbvCI BglI BglII Bme1580I
BmrI BmtI BplI BpmI Bpu10I BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstXI BstYI
Bsu36I BtgI BtsI ClaI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NheI PasI PflMI PfoI PpiI PpuMI
PshAI PspOMI PstI PvuII SacI SacII SfcI StuI
StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI ApoI AscI AseI AsiSI AvrII BamHI
BbeI BcgI BciVI BclI BfrBI BlpI BmgBI BpuEI
BsaI BsaAI BsmBI BspHI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgZI CspCI DraI EcoRI EcoRV
FseI FspAI HindIII HpaI KasI KpnI MfeI MluI
NarI NdeI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PsiI PspXI PsrI PvuI RsrII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XcmI XhoI XmaI ZraI