Restriction map for
pFN21AB6269
(Linear) (Six-base) MAPSORT of: KIBB6269.seq Check: 3380 from: 1 to: 2082
KIBB6269.orf
With 182 enzymes: SgfI *
March 22, 2012 15:34 ..
AccI GT'mk_AC
Cuts at: 0 83 2082
Size: 83 1999
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 652 875 897 2082
Size: 31 621 223 22 1185
Fragments arranged by size:
1185 621 223 31 22
AflII C'TTAA_G
Cuts at: 0 1858 2082
Size: 1858 224
AflIII A'CryG_T
Cuts at: 0 1476 2082
Size: 1476 606
AgeI A'CCGG_T
Cuts at: 0 1098 2082
Size: 1098 984
AleI CACnn'nnGTG
Cuts at: 0 319 2082
Size: 319 1763
AlwNI CAG_nnn'CTG
Cuts at: 0 1249 1911 2082
Size: 1249 662 171
Fragments arranged by size:
1249 662 171
ApaLI G'TGCA_C
Cuts at: 0 337 2082
Size: 337 1745
ApoI r'AATT_y
Cuts at: 0 822 885 1035 1812 2082
Size: 822 63 150 777 270
Fragments arranged by size:
822 777 270 150 63
AvaI C'yCGr_G
Cuts at: 0 192 259 292 470 2082
Size: 192 67 33 178 1612
Fragments arranged by size:
1612 192 178 67 33
BanI G'GyrC_C
Cuts at: 0 385 418 516 540 2082
Size: 385 33 98 24 1542
Fragments arranged by size:
1542 385 98 33 24
BanII G_rGCy'C
Cuts at: 0 77 2082
Size: 77 2005
BbeI G_GCGC'C
Cuts at: 0 389 422 2082
Size: 389 33 1660
Fragments arranged by size:
1660 389 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 794 1205 1592 2082
Size: 794 411 387 490
Fragments arranged by size:
794 490 411 387
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1765 1799 2082
Size: 1765 34 283
Fragments arranged by size:
1765 283 34
BfrBI ATG'CAT
Cuts at: 0 328 2082
Size: 328 1754
BglII A'GATC_T
Cuts at: 0 90 1461 2082
Size: 90 1371 621
Fragments arranged by size:
1371 621 90
BlpI GC'TnA_GC
Cuts at: 0 61 175 2082
Size: 61 114 1907
Fragments arranged by size:
1907 114 61
Bme1580I G_kGCm'C
Cuts at: 0 341 2082
Size: 341 1741
BmrI ACTGGGnnnn_n'
Cuts at: 0 723 1409 2082
Size: 723 686 673
Fragments arranged by size:
723 686 673
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 855 2082
Size: 855 1227
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 1975 2082
Size: 850 1125 107
Fragments arranged by size:
1125 850 107
Bpu10I CC'TnA_GC
Cuts at: 0 536 1055 2082
Size: 536 519 1027
Fragments arranged by size:
1027 536 519
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1623 2082
Size: 1623 459
BsaAI yAC'GTr
Cuts at: 0 1479 2082
Size: 1479 603
BsaBI GATnn'nnATC
Cuts at: 0 938 2082
Size: 938 1144
BsaHI Gr'CG_yC
Cuts at: 0 386 419 497 2082
Size: 386 33 78 1585
Fragments arranged by size:
1585 386 78 33
BsaWI w'CCGG_w
Cuts at: 0 406 1098 2082
Size: 406 692 984
Fragments arranged by size:
984 692 406
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 425 523 583 2082
Size: 425 98 60 1499
Fragments arranged by size:
1499 425 98 60
BseYI C'CCAG_C
Cuts at: 0 617 2082
Size: 617 1465
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 469 1499 2082
Size: 28 441 1030 583
Fragments arranged by size:
1030 583 441 28
BsiEI CG_ry'CG
Cuts at: 0 29 2082
Size: 29 2053
BsiHKAI G_wGCw'C
Cuts at: 0 341 2082
Size: 341 1741
Bsp1286I G_dGCh'C
Cuts at: 0 77 341 2082
Size: 77 264 1741
Fragments arranged by size:
1741 264 77
BspHI T'CATG_A
Cuts at: 0 945 1374 1926 2082
Size: 945 429 552 156
Fragments arranged by size:
945 552 429 156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 107 2082
Size: 107 1975
BsrBI CCG'CTC
Cuts at: 0 475 2082
Size: 475 1607
BsrFI r'CCGG_y
Cuts at: 0 28 415 1098 2082
Size: 28 387 683 984
Fragments arranged by size:
984 683 387 28
BssHII G'CGCG_C
Cuts at: 0 207 285 287 2082
Size: 207 78 2 1795
Fragments arranged by size:
1795 207 78 2
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2034 2082
Size: 2034 48
BstYI r'GATC_y
Cuts at: 0 90 987 1461 2082
Size: 90 897 474 621
Fragments arranged by size:
897 621 474 90
Bsu36I CC'TnA_GG
Cuts at: 0 2043 2082
Size: 2043 39
BtgI C'CryG_G
Cuts at: 0 372 521 1455 1543 2082
Size: 372 149 934 88 539
Fragments arranged by size:
934 539 372 149 88
ClaI AT'CG_AT
Cuts at: 0 1217 2082
Size: 1217 865
DraIII CAC_nnn'GTG
Cuts at: 0 345 1320 2082
Size: 345 975 762
Fragments arranged by size:
975 762 345
DrdI GACnn_nn'nnGTC
Cuts at: 0 1008 1723 2082
Size: 1008 715 359
Fragments arranged by size:
1008 715 359
EaeI y'GGCC_r
Cuts at: 0 26 155 1190 2082
Size: 26 129 1035 892
Fragments arranged by size:
1035 892 129 26
EagI C'GGCC_G
Cuts at: 0 26 2082
Size: 26 2056
EarI CTCTTCn'nnn_
Cuts at: 0 500 863 978 1746 2082
Size: 500 363 115 768 336
Fragments arranged by size:
768 500 363 336 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 153 569 2082
Size: 153 416 1513
Fragments arranged by size:
1513 416 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 652 850 875 897 1975 2082
Size: 31 621 198 25 22 1078 107
Fragments arranged by size:
1078 621 198 107 31 25 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 124 860 2082
Size: 124 736 1222
Fragments arranged by size:
1222 736 124
EcoO109I rG'GnC_Cy
Cuts at: 0 297 465 2082
Size: 297 168 1617
Fragments arranged by size:
1617 297 168
EcoRI G'AATT_C
Cuts at: 0 885 2082
Size: 885 1197
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1747 2082
Size: 1747 335
FspI TGC'GCA
Cuts at: 0 1775 2082
Size: 1775 307
HaeII r_GCGC'y
Cuts at: 0 389 422 2082
Size: 389 33 1660
Fragments arranged by size:
1660 389 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1345 1377 2082
Size: 1345 32 705
Fragments arranged by size:
1345 705 32
HincII GTy'rAC
Cuts at: 0 1104 2082
Size: 1104 978
HindIII A'AGCT_T
Cuts at: 0 1420 1628 2082
Size: 1420 208 454
Fragments arranged by size:
1420 454 208
KasI G'GCGC_C
Cuts at: 0 385 418 2082
Size: 385 33 1664
Fragments arranged by size:
1664 385 33
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1015 2082
Size: 1015 1067
MscI TGG'CCA
Cuts at: 0 1192 2082
Size: 1192 890
MslI CAynn'nnrTG
Cuts at: 0 319 758 902 2082
Size: 319 439 144 1180
Fragments arranged by size:
1180 439 319 144
MspA1I CmG'CkG
Cuts at: 0 270 523 2082
Size: 270 253 1559
Fragments arranged by size:
1559 270 253
NaeI GCC'GGC
Cuts at: 0 30 417 2082
Size: 30 387 1665
Fragments arranged by size:
1665 387 30
NarI GG'CG_CC
Cuts at: 0 386 419 2082
Size: 386 33 1663
Fragments arranged by size:
1663 386 33
NcoI C'CATG_G
Cuts at: 0 1455 2082
Size: 1455 627
NdeI CA'TA_TG
Cuts at: 0 1112 2082
Size: 1112 970
NgoMIV G'CCGG_C
Cuts at: 0 28 415 2082
Size: 28 387 1667
Fragments arranged by size:
1667 387 28
NsiI A_TGCA'T
Cuts at: 0 330 2082
Size: 330 1752
NspI r_CATG'y
Cuts at: 0 1671 1695 2082
Size: 1671 24 387
Fragments arranged by size:
1671 387 24
PfoI T'CCnGG_A
Cuts at: 0 275 1068 2082
Size: 275 793 1014
Fragments arranged by size:
1014 793 275
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 2082
Size: 124 32 1926
Fragments arranged by size:
1926 124 32
PpuMI rG'GwC_Cy
Cuts at: 0 297 465 2082
Size: 297 168 1617
Fragments arranged by size:
1617 297 168
PstI C_TGCA'G
Cuts at: 0 128 1031 2082
Size: 128 903 1051
Fragments arranged by size:
1051 903 128
PvuII CAG'CTG
Cuts at: 0 270 2082
Size: 270 1812
SacII CC_GC'GG
Cuts at: 0 524 2082
Size: 524 1558
SanDI GG'GwC_CC
Cuts at: 0 297 465 2082
Size: 297 168 1617
Fragments arranged by size:
1617 297 168
SfcI C'TryA_G
Cuts at: 0 124 1027 1337 1391 1649 2082
Size: 124 903 310 54 258 433
Fragments arranged by size:
903 433 310 258 124 54
SfoI GGC'GCC
Cuts at: 0 387 420 2082
Size: 387 33 1662
Fragments arranged by size:
1662 387 33
SmlI C'TyrA_G
Cuts at: 0 1602 1858 2082
Size: 1602 256 224
Fragments arranged by size:
1602 256 224
SphI G_CATG'C
Cuts at: 0 1671 2082
Size: 1671 411
StyI C'CwwG_G
Cuts at: 0 227 491 1455 1956 2082
Size: 227 264 964 501 126
Fragments arranged by size:
964 501 264 227 126
XbaI T'CTAG_A
Cuts at: 0 739 2082
Size: 739 1343
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2034 2082
Size: 2034 48
XmnI GAAnn'nnTTC
Cuts at: 0 1022 1538 2082
Size: 1022 516 544
Fragments arranged by size:
1022 544 516
Enzymes that do cut:
AccI AcuI AflII AflIII AgeI AleI AlwNI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BcgI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BstXI
BstYI Bsu36I BtgI ClaI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PfoI PpiI PpuMI PstI PvuII SacII SanDI SfcI
SfoI SmlI SphI StyI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AhdI AlfI AloI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BciVI BclI BglI BmgBI BmtI BsaI BsaXI BsiWI
BsmI BsmBI BspEI BsrDI BsrGI BssSI BstAPI BstBI
BstEII BstZ17I BtgZI BtsI CspCI DraI EcoICRI EcoRV
FseI FspAI HpaI KpnI MfeI MluI NheI NotI
NruI PacI PasI PciI PflMI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI TaqII TatI
TstI Tth111I XhoI XmaI ZraI