Restriction map for
pFN21AB6266
(Linear) (Six-base) MAPSORT of: KIBB6266.seq Check: 1939 from: 1 to: 2310
KIBB6266.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2093 2310
Size: 2093 217
AatII G_ACGT'C
Cuts at: 0 1714 2310
Size: 1714 596
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 2310
Size: 433 1877
AflIII A'CryG_T
Cuts at: 0 1187 1491 1493 2310
Size: 1187 304 2 817
Fragments arranged by size:
1187 817 304 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 53 575 2310
Size: 53 522 1735
Fragments arranged by size:
1735 522 53
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 85 117 404 436 2310
Size: 85 32 287 32 1874
Fragments arranged by size:
1874 287 85 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 105 1165 1925 2310
Size: 105 1060 760 385
Fragments arranged by size:
1060 760 385 105
ApaLI G'TGCA_C
Cuts at: 0 266 2310
Size: 266 2044
ApoI r'AATT_y
Cuts at: 0 94 655 2113 2310
Size: 94 561 1458 197
Fragments arranged by size:
1458 561 197 94
AvaI C'yCGr_G
Cuts at: 0 472 803 2271 2310
Size: 472 331 1468 39
Fragments arranged by size:
1468 472 331 39
BanI G'GyrC_C
Cuts at: 0 17 988 1882 2310
Size: 17 971 894 428
Fragments arranged by size:
971 894 428 17
BanII G_rGCy'C
Cuts at: 0 1558 2310
Size: 1558 752
BglI GCCn_nnn'nGGC
Cuts at: 0 877 1219 1231 1544 2310
Size: 877 342 12 313 766
Fragments arranged by size:
877 766 342 313 12
BglII A'GATC_T
Cuts at: 0 1976 2310
Size: 1976 334
BlpI GC'TnA_GC
Cuts at: 0 389 2227 2310
Size: 389 1838 83
Fragments arranged by size:
1838 389 83
Bme1580I G_kGCm'C
Cuts at: 0 270 592 991 1094 2310
Size: 270 322 399 103 1216
Fragments arranged by size:
1216 399 322 270 103
BmrI ACTGGGnnnn_n'
Cuts at: 0 412 2310
Size: 412 1898
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 684 986 1016 2310
Size: 684 302 30 1294
Fragments arranged by size:
1294 684 302 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1092 2310
Size: 1092 1218
BsaI GGTCTCn'nnnn_
Cuts at: 0 1024 2310
Size: 1024 1286
BsaAI yAC'GTr
Cuts at: 0 1494 2055 2310
Size: 1494 561 255
Fragments arranged by size:
1494 561 255
BsaHI Gr'CG_yC
Cuts at: 0 25 1391 1711 2310
Size: 25 1366 320 599
Fragments arranged by size:
1366 599 320 25
BsaWI w'CCGG_w
Cuts at: 0 80 2310
Size: 80 2230
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1228 1258 1281 1311 1444 1474 1696 1726
Size: 1228 30 23 30 133 30 222 30
Cuts at: 1726 1954 1984 2310
Size: 228 30 326
Fragments arranged by size:
1228 326 228 222 133 30 30 30
30 30 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 864 2152 2310
Size: 864 1288 158
Fragments arranged by size:
1288 864 158
BseYI C'CCAG_C
Cuts at: 0 1565 2310
Size: 1565 745
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1611 1792 2035 2065 2310
Size: 1611 181 243 30 245
Fragments arranged by size:
1611 245 243 181 30
BsiEI CG_ry'CG
Cuts at: 0 1223 2310
Size: 1223 1087
BsiHKAI G_wGCw'C
Cuts at: 0 270 510 569 2310
Size: 270 240 59 1741
Fragments arranged by size:
1741 270 240 59
BsmI GAATG_Cn'
Cuts at: 0 363 2310
Size: 363 1947
Bsp1286I G_dGCh'C
Cuts at: 0 270 510 569 592 991 1094 1558 2310
Size: 270 240 59 23 399 103 464 752
Fragments arranged by size:
752 464 399 270 240 103 59 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1338 1940 2093 2310
Size: 1338 602 153 217
Fragments arranged by size:
1338 602 217 153
BsrDI GCAATG_nn'
Cuts at: 0 2043 2310
Size: 2043 267
BsrFI r'CCGG_y
Cuts at: 0 14 484 2310
Size: 14 470 1826
Fragments arranged by size:
1826 470 14
BsrGI T'GTAC_A
Cuts at: 0 1496 2310
Size: 1496 814
BssSI C'ACGA_G
Cuts at: 0 1485 2310
Size: 1485 825
BstAPI GCAn_nnn'nTGC
Cuts at: 0 381 790 2310
Size: 381 409 1520
Fragments arranged by size:
1520 409 381
BstEII G'GTnAC_C
Cuts at: 0 339 2310
Size: 339 1971
BstYI r'GATC_y
Cuts at: 0 1803 1976 2310
Size: 1803 173 334
Fragments arranged by size:
1803 334 173
BtgI C'CryG_G
Cuts at: 0 344 408 866 1217 1226 1250 1446 2310
Size: 344 64 458 351 9 24 196 864
Fragments arranged by size:
864 458 351 344 196 64 24 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 746 1776 2310
Size: 746 1030 534
Fragments arranged by size:
1030 746 534
BtsI GCAGTG_nn'
Cuts at: 0 486 1094 2024 2310
Size: 486 608 930 286
Fragments arranged by size:
930 608 486 286
DraIII CAC_nnn'GTG
Cuts at: 0 183 2310
Size: 183 2127
DrdI GACnn_nn'nnGTC
Cuts at: 0 1915 2310
Size: 1915 395
EaeI y'GGCC_r
Cuts at: 0 405 1220 1739 2310
Size: 405 815 519 571
Fragments arranged by size:
815 571 519 405
EagI C'GGCC_G
Cuts at: 0 1220 2310
Size: 1220 1090
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1717 2190 2310
Size: 1717 473 120
Fragments arranged by size:
1717 473 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 684 986 1016 2310
Size: 433 251 302 30 1294
Fragments arranged by size:
1294 433 302 251 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1936 2310
Size: 1936 374
EcoO109I rG'GnC_Cy
Cuts at: 0 765 1671 1922 1929 2310
Size: 765 906 251 7 381
Fragments arranged by size:
906 765 381 251 7
EcoRI G'AATT_C
Cuts at: 0 94 2310
Size: 94 2216
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 905 1782 2310
Size: 905 877 528
Fragments arranged by size:
905 877 528
HaeII r_GCGC'y
Cuts at: 0 998 1166 1208 1245 2310
Size: 998 168 42 37 1065
Fragments arranged by size:
1065 998 168 42 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 682 714 1394 1426 1696 1728 1954 1986
Size: 682 32 680 32 270 32 226 32
Cuts at: 1986 2203 2235 2310
Size: 217 32 75
Fragments arranged by size:
682 680 270 226 217 75 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1053 2310
Size: 1053 1257
HpaI GTT'AAC
Cuts at: 0 1053 2310
Size: 1053 1257
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1708 2310
Size: 1708 602
MscI TGG'CCA
Cuts at: 0 407 1741 2310
Size: 407 1334 569
Fragments arranged by size:
1334 569 407
MslI CAynn'nnrTG
Cuts at: 0 220 557 1795 2087 2242 2310
Size: 220 337 1238 292 155 68
Fragments arranged by size:
1238 337 292 220 155 68
MspA1I CmG'CkG
Cuts at: 0 1066 1219 1859 1950 2079 2310
Size: 1066 153 640 91 129 231
Fragments arranged by size:
1066 640 231 153 129 91
NaeI GCC'GGC
Cuts at: 0 16 486 2310
Size: 16 470 1824
Fragments arranged by size:
1824 470 16
NcoI C'CATG_G
Cuts at: 0 344 1250 2310
Size: 344 906 1060
Fragments arranged by size:
1060 906 344
NdeI CA'TA_TG
Cuts at: 0 204 2310
Size: 204 2106
NgoMIV G'CCGG_C
Cuts at: 0 14 484 2310
Size: 14 470 1826
Fragments arranged by size:
1826 470 14
NotI GC'GGCC_GC
Cuts at: 0 1220 2310
Size: 1220 1090
NspI r_CATG'y
Cuts at: 0 978 1191 1423 2310
Size: 978 213 232 887
Fragments arranged by size:
978 887 232 213
PasI CC'CwG_GG
Cuts at: 0 1318 1986 2310
Size: 1318 668 324
Fragments arranged by size:
1318 668 324
PciI A'CATG_T
Cuts at: 0 1187 2310
Size: 1187 1123
PflMI CCAn_nnn'nTGG
Cuts at: 0 1892 2310
Size: 1892 418
PfoI T'CCnGG_A
Cuts at: 0 65 456 2310
Size: 65 391 1854
Fragments arranged by size:
1854 391 65
PmlI CAC'GTG
Cuts at: 0 1494 2055 2310
Size: 1494 561 255
Fragments arranged by size:
1494 561 255
PpuMI rG'GwC_Cy
Cuts at: 0 765 1671 1922 1929 2310
Size: 765 906 251 7 381
Fragments arranged by size:
906 765 381 251 7
PstI C_TGCA'G
Cuts at: 0 42 110 1356 1780 1955 2310
Size: 42 68 1246 424 175 355
Fragments arranged by size:
1246 424 355 175 68 42
PvuII CAG'CTG
Cuts at: 0 1066 1859 1950 2079 2310
Size: 1066 793 91 129 231
Fragments arranged by size:
1066 793 231 129 91
SacII CC_GC'GG
Cuts at: 0 1220 2310
Size: 1220 1090
SbfI CC_TGCA'GG
Cuts at: 0 110 2310
Size: 110 2200
ScaI AGT'ACT
Cuts at: 0 1656 2145 2310
Size: 1656 489 165
Fragments arranged by size:
1656 489 165
SfcI C'TryA_G
Cuts at: 0 38 106 1352 1776 1951 2288 2310
Size: 38 68 1246 424 175 337 22
Fragments arranged by size:
1246 424 337 175 68 38 22
SmaI CCC'GGG
Cuts at: 0 805 2310
Size: 805 1505
SmlI C'TyrA_G
Cuts at: 0 1071 2310
Size: 1071 1239
SpeI A'CTAG_T
Cuts at: 0 1821 2310
Size: 1821 489
SphI G_CATG'C
Cuts at: 0 1423 2310
Size: 1423 887
StuI AGG'CCT
Cuts at: 0 1007 2310
Size: 1007 1303
StyI C'CwwG_G
Cuts at: 0 344 1250 1757 2310
Size: 344 906 507 553
Fragments arranged by size:
906 553 507 344
TatI w'GTAC_w
Cuts at: 0 1496 1654 2143 2255 2310
Size: 1496 158 489 112 55
Fragments arranged by size:
1496 489 158 112 55
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 260 292 1444 1476 2310
Size: 260 32 1152 32 834
Fragments arranged by size:
1152 834 260 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1063 1671 2310
Size: 1063 608 639
Fragments arranged by size:
1063 639 608
XmaI C'CCGG_G
Cuts at: 0 803 2310
Size: 803 1507
ZraI GAC'GTC
Cuts at: 0 1712 2310
Size: 1712 598
Enzymes that do cut:
AarI AatII AcuI AflIII AlfI AloI AlwNI ApaLI
ApoI AvaI BanI BanII BglI BglII BlpI Bme1580I
BmrI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstYI BtgI
BtgZI BtsI DraIII DrdI EaeI EagI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HpaI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NotI NspI PasI PciI PflMI PfoI PmlI PpuMI
PstI PvuII SacII SbfI ScaI SfcI SmaI SmlI
SpeI SphI StuI StyI TatI TstI XcmI XmaI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AleI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BmgBI BmtI
BplI Bpu10I BsaBI BsiWI BsmBI BspEI BspHI BsrBI
BssHII BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraI
EarI EcoICRI EcoRV FseI FspI FspAI HindIII KasI
KpnI MfeI MluI NarI NheI NruI NsiI PacI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SalI SanDI SapI SexAI SfiI SfoI
SgfI SgrAI SnaBI SrfI SspI SwaI TaqII Tth111I
XbaI XhoI XmnI