Restriction map for
pFN21AB6265
(Linear) (Six-base) MAPSORT of: KIBB6265.seq Check: 1176 from: 1 to: 2070
KIBB6265.orf
With 182 enzymes: SgfI *
March 22, 2012 15:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 584 1613 1664 1808 1870 2070
Size: 117 467 1029 51 144 62 200
Fragments arranged by size:
1029 467 200 144 117 62 51
AflII C'TTAA_G
Cuts at: 0 1348 2070
Size: 1348 722
AlwNI CAG_nnn'CTG
Cuts at: 0 1950 2070
Size: 1950 120
ApaLI G'TGCA_C
Cuts at: 0 110 2070
Size: 110 1960
ApoI r'AATT_y
Cuts at: 0 827 1050 1837 2070
Size: 827 223 787 233
Fragments arranged by size:
827 787 233 223
AvaI C'yCGr_G
Cuts at: 0 1855 2070
Size: 1855 215
AvrII C'CTAG_G
Cuts at: 0 504 2070
Size: 504 1566
BanII G_rGCy'C
Cuts at: 0 1387 1667 1698 1856 2070
Size: 1387 280 31 158 214
Fragments arranged by size:
1387 280 214 158 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 278 2070
Size: 278 1792
BbvCI CC'TCA_GC
Cuts at: 0 908 2070
Size: 908 1162
BciVI GTATCCnnnnn_n'
Cuts at: 0 1638 2070
Size: 1638 432
BlpI GC'TnA_GC
Cuts at: 0 861 2070
Size: 861 1209
Bme1580I G_kGCm'C
Cuts at: 0 114 2070
Size: 114 1956
BmgBI CAC'GTC
Cuts at: 0 149 2070
Size: 149 1921
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 920 2026 2070
Size: 215 705 1106 44
Fragments arranged by size:
1106 705 215 44
Bpu10I CC'TnA_GC
Cuts at: 0 908 2070
Size: 908 1162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 1640 2070
Size: 258 1382 430
Fragments arranged by size:
1382 430 258
BsaI GGTCTCn'nnnn_
Cuts at: 0 746 2070
Size: 746 1324
BsaBI GATnn'nnATC
Cuts at: 0 1991 2070
Size: 1991 79
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1184 1529 2070
Size: 1184 345 541
Fragments arranged by size:
1184 541 345
BseYI C'CCAG_C
Cuts at: 0 711 1585 2070
Size: 711 874 485
Fragments arranged by size:
874 711 485
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1547 2070
Size: 1547 523
BsiHKAI G_wGCw'C
Cuts at: 0 114 954 1305 1856 2070
Size: 114 840 351 551 214
Fragments arranged by size:
840 551 351 214 114
Bsp1286I G_dGCh'C
Cuts at: 0 114 954 1305 1387 1667 1698 1856 2070
Size: 114 840 351 82 280 31 158 214
Fragments arranged by size:
840 351 280 214 158 114 82 31
BsrDI GCAATG_nn'
Cuts at: 0 884 896 1922 2070
Size: 884 12 1026 148
Fragments arranged by size:
1026 884 148 12
BsrGI T'GTAC_A
Cuts at: 0 1157 2070
Size: 1157 913
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1950 2070
Size: 1950 120
BstXI CCAn_nnnn'nTGG
Cuts at: 0 891 2070
Size: 891 1179
BtgI C'CryG_G
Cuts at: 0 1123 2070
Size: 1123 947
BtsI GCAGTG_nn'
Cuts at: 0 1948 2070
Size: 1948 122
ClaI AT'CG_AT
Cuts at: 0 943 2070
Size: 943 1127
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 637 672 2070
Size: 637 35 1398
Fragments arranged by size:
1398 637 35
DraI TTT'AAA
Cuts at: 0 1881 2070
Size: 1881 189
DrdI GACnn_nn'nnGTC
Cuts at: 0 241 2070
Size: 241 1829
EarI CTCTTCn'nnn_
Cuts at: 0 603 926 1655 1700 2070
Size: 603 323 729 45 370
Fragments arranged by size:
729 603 370 323 45
EcoICRI GAG'CTC
Cuts at: 0 1854 2070
Size: 1854 216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 215 584 920 1613 1664 1808 1870
Size: 117 98 369 336 693 51 144 62
Cuts at: 1870 2026 2070
Size: 156 44
Fragments arranged by size:
693 369 336 156 144 117 98 62
51 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1511 1595 2070
Size: 1511 84 475
Fragments arranged by size:
1511 475 84
EcoO109I rG'GnC_Cy
Cuts at: 0 1519 2070
Size: 1519 551
EcoRI G'AATT_C
Cuts at: 0 827 2070
Size: 827 1243
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 147 2070
Size: 147 1923
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 612 644 925 957 1449 1481 1612 1644
Size: 612 32 281 32 492 32 131 32
Cuts at: 1644 2005 2037 2070
Size: 361 32 33
Fragments arranged by size:
612 492 361 281 131 33 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1434 2070
Size: 1434 636
HindIII A'AGCT_T
Cuts at: 0 608 1902 2070
Size: 608 1294 168
Fragments arranged by size:
1294 608 168
MfeI C'AATT_G
Cuts at: 0 1216 2070
Size: 1216 854
MslI CAynn'nnrTG
Cuts at: 0 889 2070
Size: 889 1181
PsiI TTA'TAA
Cuts at: 0 1820 2070
Size: 1820 250
SacI G_AGCT'C
Cuts at: 0 1856 2070
Size: 1856 214
SapI GCTCTTCn'nnn_
Cuts at: 0 926 1655 2070
Size: 926 729 415
Fragments arranged by size:
926 729 415
SfcI C'TryA_G
Cuts at: 0 23 762 1306 2070
Size: 23 739 544 764
Fragments arranged by size:
764 739 544 23
SmlI C'TyrA_G
Cuts at: 0 273 1348 1655 1855 2070
Size: 273 1075 307 200 215
Fragments arranged by size:
1075 307 273 215 200
SspI AAT'ATT
Cuts at: 0 261 1068 2070
Size: 261 807 1002
Fragments arranged by size:
1002 807 261
StyI C'CwwG_G
Cuts at: 0 504 1273 2070
Size: 504 769 797
Fragments arranged by size:
797 769 504
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1346 2070
Size: 1346 724
TatI w'GTAC_w
Cuts at: 0 603 1015 1089 1150 1157 2070
Size: 603 412 74 61 7 913
Fragments arranged by size:
913 603 412 74 61 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 389 421 2070
Size: 389 32 1649
Fragments arranged by size:
1649 389 32
XbaI T'CTAG_A
Cuts at: 0 1291 2070
Size: 1291 779
XhoI C'TCGA_G
Cuts at: 0 1855 2070
Size: 1855 215
XmnI GAAnn'nnTTC
Cuts at: 0 597 1445 1676 2070
Size: 597 848 231 394
Fragments arranged by size:
848 597 394 231
Enzymes that do cut:
AcuI AflII AlwNI ApaLI ApoI AvaI AvrII BanII
BbsI BbvCI BciVI BlpI Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaI BsaBI BseRI BseYI BsgI BsiHKAI Bsp1286I
BsrDI BsrGI BstAPI BstXI BtgI BtsI ClaI CspCI
DraI DrdI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI Hin4I HincII HindIII MfeI MslI PsiI SacI
SapI SfcI SmlI SspI StyI TaqII TatI TstI
XbaI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
BaeI BamHI BanI BbeI BcgI BclI BfrBI BglI
BglII BmrI BmtI BplI BsaAI BsaHI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI DraIII EaeI EagI EciI EcoRV FseI FspI
FspAI HaeII HpaI KasI KpnI MluI MmeI MscI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI Tth111I XcmI XmaI
ZraI