Restriction map for
pFN21AB6263
(Linear) (Six-base) MAPSORT of: KIBB6263.seq Check: 7381 from: 1 to: 2064
KIBB6263.orf
With 182 enzymes: SgfI *
December 27, 2011 16:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 361 1457 1784 2064
Size: 361 1096 327 280
Fragments arranged by size:
1096 361 327 280
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 598 677 1545 2064
Size: 338 260 79 868 519
Fragments arranged by size:
868 519 338 260 79
AfeI AGC'GCT
Cuts at: 0 1585 2064
Size: 1585 479
AflIII A'CryG_T
Cuts at: 0 734 883 1392 2064
Size: 734 149 509 672
Fragments arranged by size:
734 672 509 149
AhdI GACnn_n'nnGTC
Cuts at: 0 470 2064
Size: 470 1594
AleI CACnn'nnGTG
Cuts at: 0 122 1292 1397 1463 1490 2064
Size: 122 1170 105 66 27 574
Fragments arranged by size:
1170 574 122 105 66 27
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1907 2064
Size: 1907 157
AlwNI CAG_nnn'CTG
Cuts at: 0 1977 2041 2064
Size: 1977 64 23
Fragments arranged by size:
1977 64 23
ApaI G_GGCC'C
Cuts at: 0 395 556 1604 2064
Size: 395 161 1048 460
Fragments arranged by size:
1048 460 395 161
ApaLI G'TGCA_C
Cuts at: 0 1099 1699 1737 2064
Size: 1099 600 38 327
Fragments arranged by size:
1099 600 327 38
AvaI C'yCGr_G
Cuts at: 0 619 1335 2047 2064
Size: 619 716 712 17
Fragments arranged by size:
716 712 619 17
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 848 881 2064
Size: 848 33 1183
Fragments arranged by size:
1183 848 33
BanI G'GyrC_C
Cuts at: 0 661 864 1325 2064
Size: 661 203 461 739
Fragments arranged by size:
739 661 461 203
BanII G_rGCy'C
Cuts at: 0 47 395 497 556 823 1209 1604 1912
Size: 47 348 102 59 267 386 395 308
Cuts at: 1912 2021 2064
Size: 109 43
Fragments arranged by size:
395 386 348 308 267 109 102 59
47 43
BbsI GAAGACnn'nnnn_
Cuts at: 0 1038 2064
Size: 1038 1026
BbvCI CC'TCA_GC
Cuts at: 0 901 2064
Size: 901 1163
BclI T'GATC_A
Cuts at: 0 1190 1847 2064
Size: 1190 657 217
Fragments arranged by size:
1190 657 217
BfrBI ATG'CAT
Cuts at: 0 1222 2064
Size: 1222 842
BglI GCCn_nnn'nGGC
Cuts at: 0 642 1346 2064
Size: 642 704 718
Fragments arranged by size:
718 704 642
BglII A'GATC_T
Cuts at: 0 212 2064
Size: 212 1852
BlpI GC'TnA_GC
Cuts at: 0 800 833 2064
Size: 800 33 1231
Fragments arranged by size:
1231 800 33
Bme1580I G_kGCm'C
Cuts at: 0 395 556 638 666 1103 1328 1604 1703
Size: 395 161 82 28 437 225 276 99
Cuts at: 1703 1741 2064
Size: 38 323
Fragments arranged by size:
437 395 323 276 225 161 99 82
38 28
BmgBI CAC'GTC
Cuts at: 0 93 423 1704 2064
Size: 93 330 1281 360
Fragments arranged by size:
1281 360 330 93
BmrI ACTGGGnnnn_n'
Cuts at: 0 950 2064
Size: 950 1114
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 573 1926 2064
Size: 573 1353 138
Fragments arranged by size:
1353 573 138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 1024 1921 2035 2064
Size: 141 883 897 114 29
Fragments arranged by size:
897 883 141 114 29
Bpu10I CC'TnA_GC
Cuts at: 0 174 811 901 2064
Size: 174 637 90 1163
Fragments arranged by size:
1163 637 174 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 2064
Size: 625 1439
BsaAI yAC'GTr
Cuts at: 0 1052 1395 1617 2064
Size: 1052 343 222 447
Fragments arranged by size:
1052 447 343 222
BsaBI GATnn'nnATC
Cuts at: 0 1360 2064
Size: 1360 704
BsaHI Gr'CG_yC
Cuts at: 0 80 2064
Size: 80 1984
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 549 1330 2002 2064
Size: 549 781 672 62
Fragments arranged by size:
781 672 549 62
BseYI C'CCAG_C
Cuts at: 0 387 524 537 665 1151 1958 2064
Size: 387 137 13 128 486 807 106
Fragments arranged by size:
807 486 387 137 128 106 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 815 2003 2064
Size: 815 1188 61
Fragments arranged by size:
1188 815 61
BsiEI CG_ry'CG
Cuts at: 0 872 1803 2064
Size: 872 931 261
Fragments arranged by size:
931 872 261
BsiHKAI G_wGCw'C
Cuts at: 0 47 287 1057 1103 1703 1741 2064
Size: 47 240 770 46 600 38 323
Fragments arranged by size:
770 600 323 240 47 46 38
BsmI GAATG_Cn'
Cuts at: 0 880 2064
Size: 880 1184
Bsp1286I G_dGCh'C
Cuts at: 0 47 287 395 497 556 638 666 823
Size: 47 240 108 102 59 82 28 157
Cuts at: 823 1057 1103 1209 1328 1604 1703 1741 1912
Size: 234 46 106 119 276 99 38 171
Cuts at: 1912 2021 2064
Size: 109 43
Fragments arranged by size:
276 240 234 171 157 119 109 108
106 102 99 82 59 47 46 43
38 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 361 1457 1784 2064
Size: 361 1096 327 280
Fragments arranged by size:
1096 361 327 280
BsrBI CCG'CTC
Cuts at: 0 1034 2064
Size: 1034 1030
BsrFI r'CCGG_y
Cuts at: 0 432 867 1640 1999 2064
Size: 432 435 773 359 65
Fragments arranged by size:
773 435 432 359 65
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1161 2064
Size: 1161 903
BstEII G'GTnAC_C
Cuts at: 0 247 2064
Size: 247 1817
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1492 2064
Size: 1492 572
BstYI r'GATC_y
Cuts at: 0 212 1793 2064
Size: 212 1581 271
Fragments arranged by size:
1581 271 212
Bsu36I CC'TnA_GG
Cuts at: 0 264 746 1609 2064
Size: 264 482 863 455
Fragments arranged by size:
863 482 455 264
BtgI C'CryG_G
Cuts at: 0 1290 1682 1822 2064
Size: 1290 392 140 242
Fragments arranged by size:
1290 392 242 140
BtsI GCAGTG_nn'
Cuts at: 0 189 2064
Size: 189 1875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1272 1307 2064
Size: 1272 35 757
Fragments arranged by size:
1272 757 35
DraIII CAC_nnn'GTG
Cuts at: 0 27 1107 1745 2064
Size: 27 1080 638 319
Fragments arranged by size:
1080 638 319 27
EaeI y'GGCC_r
Cuts at: 0 375 1619 1968 2064
Size: 375 1244 349 96
Fragments arranged by size:
1244 375 349 96
EarI CTCTTCn'nnn_
Cuts at: 0 443 2064
Size: 443 1621
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1066 1930 2064
Size: 1066 864 134
Fragments arranged by size:
1066 864 134
EcoICRI GAG'CTC
Cuts at: 0 45 2064
Size: 45 2019
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 338 598 677 1024 1545 1921 2035
Size: 141 197 260 79 347 521 376 114
Cuts at: 2035 2064
Size: 29
Fragments arranged by size:
521 376 347 260 197 141 114 79
29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1939 2064
Size: 1939 125
EcoO109I rG'GnC_Cy
Cuts at: 0 99 391 392 521 552 553 644 1556
Size: 99 292 1 129 31 1 91 912
Cuts at: 1556 1600 1601 2064
Size: 44 1 463
Fragments arranged by size:
912 463 292 129 99 91 44 31
1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 603 999 1191 2064
Size: 603 396 192 873
Fragments arranged by size:
873 603 396 192
FseI GG_CCGG'CC
Cuts at: 0 1644 2064
Size: 1644 420
FspI TGC'GCA
Cuts at: 0 1453 2064
Size: 1453 611
HaeII r_GCGC'y
Cuts at: 0 1587 1882 2064
Size: 1587 295 182
Fragments arranged by size:
1587 295 182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 456 488 721 753 2064
Size: 456 32 233 32 1311
Fragments arranged by size:
1311 456 233 32 32
HindIII A'AGCT_T
Cuts at: 0 1277 2064
Size: 1277 787
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 2064
Size: 209 1855
MscI TGG'CCA
Cuts at: 0 377 1621 2064
Size: 377 1244 443
Fragments arranged by size:
1244 443 377
MslI CAynn'nnrTG
Cuts at: 0 122 1292 1379 1395 1397 1463 1490 2064
Size: 122 1170 87 16 2 66 27 574
Fragments arranged by size:
1170 574 122 87 66 27 16 2
MspA1I CmG'CkG
Cuts at: 0 57 669 799 832 862 1269 1824 2038
Size: 57 612 130 33 30 407 555 214
Cuts at: 2038 2064
Size: 26
Fragments arranged by size:
612 555 407 214 130 57 33 30
26
NaeI GCC'GGC
Cuts at: 0 434 1642 2064
Size: 434 1208 422
Fragments arranged by size:
1208 434 422
NgoMIV G'CCGG_C
Cuts at: 0 432 1640 2064
Size: 432 1208 424
Fragments arranged by size:
1208 432 424
NsiI A_TGCA'T
Cuts at: 0 1224 2064
Size: 1224 840
NspI r_CATG'y
Cuts at: 0 738 887 1226 1446 2064
Size: 738 149 339 220 618
Fragments arranged by size:
738 618 339 220 149
PasI CC'CwG_GG
Cuts at: 0 482 1941 2064
Size: 482 1459 123
Fragments arranged by size:
1459 482 123
PciI A'CATG_T
Cuts at: 0 734 883 2064
Size: 734 149 1181
Fragments arranged by size:
1181 734 149
PfoI T'CCnGG_A
Cuts at: 0 1001 2012 2064
Size: 1001 1011 52
Fragments arranged by size:
1011 1001 52
PmlI CAC'GTG
Cuts at: 0 1052 1617 2064
Size: 1052 565 447
Fragments arranged by size:
1052 565 447
PspOMI G'GGCC_C
Cuts at: 0 391 552 1600 2064
Size: 391 161 1048 464
Fragments arranged by size:
1048 464 391 161
PspXI vC'TCGA_Gb
Cuts at: 0 1335 2064
Size: 1335 729
PstI C_TGCA'G
Cuts at: 0 41 142 674 1268 1355 1411 1886 1896
Size: 41 101 532 594 87 56 475 10
Cuts at: 1896 2064
Size: 168
Fragments arranged by size:
594 532 475 168 101 87 56 41
10
PvuII CAG'CTG
Cuts at: 0 669 799 832 1269 2038 2064
Size: 669 130 33 437 769 26
Fragments arranged by size:
769 669 437 130 33 26
SacI G_AGCT'C
Cuts at: 0 47 2064
Size: 47 2017
SacII CC_GC'GG
Cuts at: 0 1825 2064
Size: 1825 239
ScaI AGT'ACT
Cuts at: 0 73 2064
Size: 73 1991
SfcI C'TryA_G
Cuts at: 0 37 138 670 1264 1351 1407 1882 1892
Size: 37 101 532 594 87 56 475 10
Cuts at: 1892 2064
Size: 172
Fragments arranged by size:
594 532 475 172 101 87 56 37
10
SmlI C'TyrA_G
Cuts at: 0 604 1335 2064
Size: 604 731 729
Fragments arranged by size:
731 729 604
SphI G_CATG'C
Cuts at: 0 1226 2064
Size: 1226 838
SspI AAT'ATT
Cuts at: 0 910 2064
Size: 910 1154
StuI AGG'CCT
Cuts at: 0 586 2064
Size: 586 1478
StyI C'CwwG_G
Cuts at: 0 640 2064
Size: 640 1424
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1438 1555 2064
Size: 1438 117 509
Fragments arranged by size:
1438 509 117
TatI w'GTAC_w
Cuts at: 0 71 2064
Size: 71 1993
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 90 354 2064
Size: 90 264 1710
Fragments arranged by size:
1710 264 90
XhoI C'TCGA_G
Cuts at: 0 1335 2064
Size: 1335 729
XmnI GAAnn'nnTTC
Cuts at: 0 1474 2064
Size: 1474 590
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AlfI AlwNI
ApaI ApaLI AvaI BaeI BanI BanII BbsI BbvCI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI
CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FseI FspI HaeII Hin4I HindIII MmeI
MscI MslI MspA1I NaeI NgoMIV NsiI NspI PasI
PciI PfoI PmlI PspOMI PspXI PstI PvuII SacI
SacII ScaI SfcI SmlI SphI SspI StuI StyI
TaqII TatI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AloI ApoI
AscI AseI AsiSI AvrII BamHI BbeI BcgI BciVI
BmtI BsaI BsaWI BsaXI BsiWI BsmBI BspEI BspHI
BsrDI BsrGI BssHII BssSI BstBI BstZ17I BtgZI ClaI
DraI DrdI EagI EcoRI EcoRV FspAI HincII HpaI
KasI KpnI MfeI MluI NarI NcoI NdeI NheI
NotI NruI PacI PflMI PmeI PpiI PpuMI PshAI
PsiI PsrI PvuI RsrII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SwaI TstI Tth111I XbaI XmaI ZraI