Restriction map for
pFN21AB6261
(Linear) (Six-base) MAPSORT of: KIBB6261.seq Check: 1512 from: 1 to: 2058
KIBB6261.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
Acc65I G'GTAC_C
Cuts at: 0 1933 2058
Size: 1933 125
AclI AA'CG_TT
Cuts at: 0 1201 2058
Size: 1201 857
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 1106 1129 2058
Size: 743 363 23 929
Fragments arranged by size:
929 743 363 23
AflII C'TTAA_G
Cuts at: 0 1923 2058
Size: 1923 135
AhdI GACnn_n'nnGTC
Cuts at: 0 453 1668 2058
Size: 453 1215 390
Fragments arranged by size:
1215 453 390
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 80 2058
Size: 80 1978
AlwNI CAG_nnn'CTG
Cuts at: 0 123 973 2058
Size: 123 850 1085
Fragments arranged by size:
1085 850 123
ApoI r'AATT_y
Cuts at: 0 685 1777 1857 2058
Size: 685 1092 80 201
Fragments arranged by size:
1092 685 201 80
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 552 585 2058
Size: 552 33 1473
Fragments arranged by size:
1473 552 33
BanI G'GyrC_C
Cuts at: 0 4 1933 2058
Size: 4 1929 125
Fragments arranged by size:
1929 125 4
BanII G_rGCy'C
Cuts at: 0 248 307 1293 1382 2058
Size: 248 59 986 89 676
Fragments arranged by size:
986 676 248 89 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 863 878 2058
Size: 863 15 1180
Fragments arranged by size:
1180 863 15
BbvCI CC'TCA_GC
Cuts at: 0 52 733 2058
Size: 52 681 1325
Fragments arranged by size:
1325 681 52
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 183 217 2058
Size: 183 34 1841
Fragments arranged by size:
1841 183 34
Bme1580I G_kGCm'C
Cuts at: 0 7 2058
Size: 7 2051
BmgBI CAC'GTC
Cuts at: 0 450 2058
Size: 450 1608
BmrI ACTGGGnnnn_n'
Cuts at: 0 871 1684 1923 2058
Size: 871 813 239 135
Fragments arranged by size:
871 813 239 135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 103 109 1592 2058
Size: 16 87 6 1483 466
Fragments arranged by size:
1483 466 87 16 6
Bpu10I CC'TnA_GC
Cuts at: 0 52 733 2058
Size: 52 681 1325
Fragments arranged by size:
1325 681 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1204 1483 2058
Size: 1204 279 575
Fragments arranged by size:
1204 575 279
BsaI GGTCTCn'nnnn_
Cuts at: 0 754 2058
Size: 754 1304
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 76 106 2058
Size: 76 30 1952
Fragments arranged by size:
1952 76 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 2058
Size: 61 1997
BseYI C'CCAG_C
Cuts at: 0 170 2058
Size: 170 1888
BsiEI CG_ry'CG
Cuts at: 0 584 2058
Size: 584 1474
BsiHKAI G_wGCw'C
Cuts at: 0 199 1259 1382 2058
Size: 199 1060 123 676
Fragments arranged by size:
1060 676 199 123
BsmI GAATG_Cn'
Cuts at: 0 1470 2058
Size: 1470 588
BsmBI CGTCTCn'nnnn_
Cuts at: 0 440 2058
Size: 440 1618
Bsp1286I G_dGCh'C
Cuts at: 0 7 199 248 307 1259 1293 1382 2058
Size: 7 192 49 59 952 34 89 676
Fragments arranged by size:
952 676 192 89 59 49 34 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 988 2058
Size: 988 1070
BsrBI CCG'CTC
Cuts at: 0 149 1265 2058
Size: 149 1116 793
Fragments arranged by size:
1116 793 149
BsrDI GCAATG_nn'
Cuts at: 0 1214 2058
Size: 1214 844
BsrGI T'GTAC_A
Cuts at: 0 1316 2058
Size: 1316 742
BssSI C'ACGA_G
Cuts at: 0 851 2058
Size: 851 1207
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1281 2058
Size: 1281 777
BstYI r'GATC_y
Cuts at: 0 267 318 377 728 1728 2058
Size: 267 51 59 351 1000 330
Fragments arranged by size:
1000 351 330 267 59 51
Bsu36I CC'TnA_GG
Cuts at: 0 2003 2058
Size: 2003 55
BtgI C'CryG_G
Cuts at: 0 521 2058
Size: 521 1537
DraI TTT'AAA
Cuts at: 0 692 1008 2058
Size: 692 316 1050
Fragments arranged by size:
1050 692 316
DraIII CAC_nnn'GTG
Cuts at: 0 868 1236 2058
Size: 868 368 822
Fragments arranged by size:
868 822 368
EarI CTCTTCn'nnn_
Cuts at: 0 719 752 2058
Size: 719 33 1306
Fragments arranged by size:
1306 719 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 228 2058
Size: 228 1830
EcoICRI GAG'CTC
Cuts at: 0 1380 2058
Size: 1380 678
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 103 109 743 1106 1129 1592 2058
Size: 16 87 6 634 363 23 463 466
Fragments arranged by size:
634 466 463 363 87 23 16 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1718 2058
Size: 1718 340
EcoO109I rG'GnC_Cy
Cuts at: 0 1494 2058
Size: 1494 564
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 355 2058
Size: 355 1703
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 439 471 985 1017 1654 1686 1825 1857
Size: 439 32 514 32 637 32 139 32
Cuts at: 1857 2020 2052 2058
Size: 163 32 6
Fragments arranged by size:
637 514 439 163 139 32 32 32
32 32 6
HindIII A'AGCT_T
Cuts at: 0 739 1361 1454 2058
Size: 739 622 93 604
Fragments arranged by size:
739 622 604 93
KpnI G_GTAC'C
Cuts at: 0 1937 2058
Size: 1937 121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 183 727 1128 2058
Size: 74 109 544 401 930
Fragments arranged by size:
930 544 401 109 74
MspA1I CmG'CkG
Cuts at: 0 60 2058
Size: 60 1998
NcoI C'CATG_G
Cuts at: 0 521 2058
Size: 521 1537
NdeI CA'TA_TG
Cuts at: 0 1050 2058
Size: 1050 1008
NspI r_CATG'y
Cuts at: 0 1445 2058
Size: 1445 613
PacI TTA_AT'TAA
Cuts at: 0 1817 2058
Size: 1817 241
PfoI T'CCnGG_A
Cuts at: 0 32 263 380 528 2058
Size: 32 231 117 148 1530
Fragments arranged by size:
1530 231 148 117 32
PpuMI rG'GwC_Cy
Cuts at: 0 1494 2058
Size: 1494 564
PsiI TTA'TAA
Cuts at: 0 325 2058
Size: 325 1733
PstI C_TGCA'G
Cuts at: 0 128 969 2058
Size: 128 841 1089
Fragments arranged by size:
1089 841 128
SacI G_AGCT'C
Cuts at: 0 1382 2058
Size: 1382 676
SfcI C'TryA_G
Cuts at: 0 124 965 2058
Size: 124 841 1093
Fragments arranged by size:
1093 841 124
SmlI C'TyrA_G
Cuts at: 0 1219 1498 1923 2058
Size: 1219 279 425 135
Fragments arranged by size:
1219 425 279 135
SspI AAT'ATT
Cuts at: 0 1342 2058
Size: 1342 716
StyI C'CwwG_G
Cuts at: 0 314 521 1140 2058
Size: 314 207 619 918
Fragments arranged by size:
918 619 314 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 184 1986 2058
Size: 184 1802 72
Fragments arranged by size:
1802 184 72
TatI w'GTAC_w
Cuts at: 0 1316 2058
Size: 1316 742
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1584 1616 1664 1696 2058
Size: 1584 32 48 32 362
Fragments arranged by size:
1584 362 48 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 877 2058
Size: 877 1181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 772 2058
Size: 772 1286
Enzymes that do cut:
Acc65I AclI AcuI AflII AhdI AlfI AlwNI ApoI
BaeI BanI BanII BbsI BbvCI BcgI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrGI BssSI BstAPI BstYI Bsu36I BtgI DraI DraIII
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I
HindIII KpnI MmeI MspA1I NcoI NdeI NspI PacI
PfoI PpuMI PsiI PstI SacI SfcI SmlI SspI
StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI AfeI AflIII AgeI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BamHI
BbeI BciVI BclI BfrBI BglI BglII BlpI BmtI
BplI BsaAI BsaBI BsaHI BsaWI BsgI BsiWI BspEI
BspHI BsrFI BssHII BstBI BstEII BstXI BstZ17I BtgZI
BtsI ClaI CspCI DrdI EaeI EagI EcoRI EcoRV
FseI FspI FspAI HaeII HincII HpaI KasI MfeI
MluI MscI MslI NaeI NarI NgoMIV NheI NotI
NruI NsiI PasI PciI PflMI PmeI PmlI PpiI
PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI XbaI XhoI XmaI XmnI ZraI