Restriction map for
pFN21AB6257
(Linear) (Six-base) MAPSORT of: KIBB6257.seq Check: 9399 from: 1 to: 2289
KIBB6257.orf
With 182 enzymes: SgfI *
July 3, 2012 16:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1903 2078 2289
Size: 1903 175 211
Fragments arranged by size:
1903 211 175
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 894 1304 2289
Size: 894 410 985
Fragments arranged by size:
985 894 410
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1040 1933 2289
Size: 1040 893 356
Fragments arranged by size:
1040 893 356
AlwNI CAG_nnn'CTG
Cuts at: 0 976 1182 1521 1848 2110 2289
Size: 976 206 339 327 262 179
Fragments arranged by size:
976 339 327 262 206 179
ApoI r'AATT_y
Cuts at: 0 196 1549 1622 2201 2289
Size: 196 1353 73 579 88
Fragments arranged by size:
1353 579 196 88 73
AseI AT'TA_AT
Cuts at: 0 832 2289
Size: 832 1457
AvaI C'yCGr_G
Cuts at: 0 574 1637 2289
Size: 574 1063 652
Fragments arranged by size:
1063 652 574
BanII G_rGCy'C
Cuts at: 0 140 461 2289
Size: 140 321 1828
Fragments arranged by size:
1828 321 140
BbsI GAAGACnn'nnnn_
Cuts at: 0 35 287 2289
Size: 35 252 2002
Fragments arranged by size:
2002 252 35
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 524 558 2289
Size: 524 34 1731
Fragments arranged by size:
1731 524 34
BclI T'GATC_A
Cuts at: 0 614 855 2289
Size: 614 241 1434
Fragments arranged by size:
1434 614 241
BlpI GC'TnA_GC
Cuts at: 0 582 2289
Size: 582 1707
BmrI ACTGGGnnnn_n'
Cuts at: 0 482 2289
Size: 482 1807
BmtI G_CTAG'C
Cuts at: 0 1039 2289
Size: 1039 1250
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1529 2289
Size: 1529 760
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 887 1040 1522 1625 1870 2054 2289
Size: 778 109 153 482 103 245 184 235
Fragments arranged by size:
778 482 245 235 184 153 109 103
Bpu10I CC'TnA_GC
Cuts at: 0 996 2289
Size: 996 1293
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2070 2289
Size: 2070 219
BsaI GGTCTCn'nnnn_
Cuts at: 0 1468 2289
Size: 1468 821
BsaBI GATnn'nnATC
Cuts at: 0 1574 2289
Size: 1574 715
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2244 2274 2289
Size: 2244 30 15
Fragments arranged by size:
2244 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 886 1834 2167 2289
Size: 139 747 948 333 122
Fragments arranged by size:
948 747 333 139 122
BseYI C'CCAG_C
Cuts at: 0 141 881 1031 1299 2050 2289
Size: 141 740 150 268 751 239
Fragments arranged by size:
751 740 268 239 150 141
BsiHKAI G_wGCw'C
Cuts at: 0 461 1138 2084 2134 2289
Size: 461 677 946 50 155
Fragments arranged by size:
946 677 461 155 50
BsmI GAATG_Cn'
Cuts at: 0 1118 1686 2289
Size: 1118 568 603
Fragments arranged by size:
1118 603 568
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1837 2289
Size: 1837 452
Bsp1286I G_dGCh'C
Cuts at: 0 140 461 1138 2084 2134 2289
Size: 140 321 677 946 50 155
Fragments arranged by size:
946 677 321 155 140 50
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1038 1903 2078 2289
Size: 1038 865 175 211
Fragments arranged by size:
1038 865 211 175
BsrDI GCAATG_nn'
Cuts at: 0 542 1049 1568 1659 2006 2289
Size: 542 507 519 91 347 283
Fragments arranged by size:
542 519 507 347 283 91
BstAPI GCAn_nnn'nTGC
Cuts at: 0 976 1965 2289
Size: 976 989 324
Fragments arranged by size:
989 976 324
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2046 2289
Size: 2046 243
BstYI r'GATC_y
Cuts at: 0 803 1718 1771 2000 2289
Size: 803 915 53 229 289
Fragments arranged by size:
915 803 289 229 53
Bsu36I CC'TnA_GG
Cuts at: 0 23 2289
Size: 23 2266
BtgI C'CryG_G
Cuts at: 0 92 965 2045 2289
Size: 92 873 1080 244
Fragments arranged by size:
1080 873 244 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2025 2289
Size: 2025 264
BtsI GCAGTG_nn'
Cuts at: 0 2012 2289
Size: 2012 277
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1563 1598 2289
Size: 1563 35 691
Fragments arranged by size:
1563 691 35
EaeI y'GGCC_r
Cuts at: 0 234 719 2289
Size: 234 485 1570
Fragments arranged by size:
1570 485 234
EarI CTCTTCn'nnn_
Cuts at: 0 116 449 2165 2219 2289
Size: 116 333 1716 54 70
Fragments arranged by size:
1716 333 116 70 54
EcoICRI GAG'CTC
Cuts at: 0 459 2289
Size: 459 1830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 887 894 1040 1304 1522 1625 1870
Size: 778 109 7 146 264 218 103 245
Cuts at: 1870 2054 2289
Size: 184 235
Fragments arranged by size:
778 264 245 235 218 184 146 109
103 7
EcoO109I rG'GnC_Cy
Cuts at: 0 1279 2289
Size: 1279 1010
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 59 544 1108 2289
Size: 59 485 564 1181
Fragments arranged by size:
1181 564 485 59
FspI TGC'GCA
Cuts at: 0 1921 1948 2289
Size: 1921 27 341
Fragments arranged by size:
1921 341 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 355 387 881 913 1204 1236 2289
Size: 355 32 494 32 291 32 1053
Fragments arranged by size:
1053 494 355 291 32 32 32
HincII GTy'rAC
Cuts at: 0 429 2289
Size: 429 1860
HindIII A'AGCT_T
Cuts at: 0 526 2289
Size: 526 1763
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1032 1434 1775 2289
Size: 1032 402 341 514
Fragments arranged by size:
1032 514 402 341
MscI TGG'CCA
Cuts at: 0 236 721 2289
Size: 236 485 1568
Fragments arranged by size:
1568 485 236
MslI CAynn'nnrTG
Cuts at: 0 610 2044 2289
Size: 610 1434 245
Fragments arranged by size:
1434 610 245
MspA1I CmG'CkG
Cuts at: 0 624 645 822 877 951 973 2289
Size: 624 21 177 55 74 22 1316
Fragments arranged by size:
1316 624 177 74 55 22 21
NcoI C'CATG_G
Cuts at: 0 92 965 2045 2289
Size: 92 873 1080 244
Fragments arranged by size:
1080 873 244 92
NdeI CA'TA_TG
Cuts at: 0 1247 2289
Size: 1247 1042
NheI G'CTAG_C
Cuts at: 0 1035 2289
Size: 1035 1254
NspI r_CATG'y
Cuts at: 0 166 1743 2289
Size: 166 1577 546
Fragments arranged by size:
1577 546 166
PasI CC'CwG_GG
Cuts at: 0 384 2289
Size: 384 1905
PflMI CCAn_nnn'nTGG
Cuts at: 0 2110 2289
Size: 2110 179
PfoI T'CCnGG_A
Cuts at: 0 1056 2289
Size: 1056 1233
PstI C_TGCA'G
Cuts at: 0 876 1016 2289
Size: 876 140 1273
Fragments arranged by size:
1273 876 140
PvuII CAG'CTG
Cuts at: 0 624 645 822 877 951 973 2289
Size: 624 21 177 55 74 22 1316
Fragments arranged by size:
1316 624 177 74 55 22 21
SacI G_AGCT'C
Cuts at: 0 461 2289
Size: 461 1828
SapI GCTCTTCn'nnn_
Cuts at: 0 449 2289
Size: 449 1840
ScaI AGT'ACT
Cuts at: 0 1975 2289
Size: 1975 314
SfcI C'TryA_G
Cuts at: 0 766 872 1012 2289
Size: 766 106 140 1277
Fragments arranged by size:
1277 766 140 106
SmlI C'TyrA_G
Cuts at: 0 2085 2289
Size: 2085 204
StyI C'CwwG_G
Cuts at: 0 92 965 1083 1092 1370 2045 2289
Size: 92 873 118 9 278 675 244
Fragments arranged by size:
873 675 278 244 118 92 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1167 1239 1248 2289
Size: 1167 72 9 1041
Fragments arranged by size:
1167 1041 72 9
TatI w'GTAC_w
Cuts at: 0 376 1871 1973 2289
Size: 376 1495 102 316
Fragments arranged by size:
1495 376 316 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2244 2276 2289
Size: 2244 32 13
Fragments arranged by size:
2244 32 13
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1090 2289
Size: 1090 1199
XmnI GAAnn'nnTTC
Cuts at: 0 1684 2289
Size: 1684 605
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApoI AseI AvaI BanII
BbsI BcgI BclI BlpI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BseYI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrDI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI EaeI EarI EcoICRI
Eco57MI EcoO109I FalI FspI Hin4I HincII HindIII MmeI
MscI MslI MspA1I NcoI NdeI NheI NspI PasI
PflMI PfoI PstI PvuII SacI SapI ScaI SfcI
SmlI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BciVI BfrBI BglI
BglII Bme1580I BmgBI BsaAI BsaHI BsaWI BsgI BsiEI
BsiWI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstZ17I ClaI DraI DraIII DrdI EagI
EciI EcoNI EcoRI EcoRV FseI FspAI HaeII HpaI
KasI KpnI MfeI MluI NaeI NarI NgoMIV NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmaI ZraI