Restriction map for
pFN21AB6255
(Linear) (Six-base) MAPSORT of: KIBB6255.seq Check: 6565 from: 1 to: 2286
KIBB6255.orf
With 182 enzymes: SgfI *
March 22, 2012 15:34 ..
AccI GT'mk_AC
Cuts at: 0 439 714 2286
Size: 439 275 1572
Fragments arranged by size:
1572 439 275
Acc65I G'GTAC_C
Cuts at: 0 32 2286
Size: 32 2254
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1023 1270 1677 2286
Size: 1023 247 407 609
Fragments arranged by size:
1023 609 407 247
AflII C'TTAA_G
Cuts at: 0 1750 2059 2286
Size: 1750 309 227
Fragments arranged by size:
1750 309 227
AflIII A'CryG_T
Cuts at: 0 1088 1139 2286
Size: 1088 51 1147
Fragments arranged by size:
1147 1088 51
AlwNI CAG_nnn'CTG
Cuts at: 0 1434 2286
Size: 1434 852
ApaI G_GGCC'C
Cuts at: 0 1209 2286
Size: 1209 1077
ApoI r'AATT_y
Cuts at: 0 200 754 1021 1290 2286
Size: 200 554 267 269 996
Fragments arranged by size:
996 554 269 267 200
AseI AT'TA_AT
Cuts at: 0 640 1595 2048 2286
Size: 640 955 453 238
Fragments arranged by size:
955 640 453 238
BanI G'GyrC_C
Cuts at: 0 32 941 1557 2286
Size: 32 909 616 729
Fragments arranged by size:
909 729 616 32
BanII G_rGCy'C
Cuts at: 0 1051 1209 2286
Size: 1051 158 1077
Fragments arranged by size:
1077 1051 158
BbvCI CC'TCA_GC
Cuts at: 0 1529 2286
Size: 1529 757
BciVI GTATCCnnnnn_n'
Cuts at: 0 1848 2286
Size: 1848 438
BglII A'GATC_T
Cuts at: 0 1535 2286
Size: 1535 751
Bme1580I G_kGCm'C
Cuts at: 0 1209 1560 2286
Size: 1209 351 726
Fragments arranged by size:
1209 726 351
BmrI ACTGGGnnnn_n'
Cuts at: 0 1941 1999 2286
Size: 1941 58 287
Fragments arranged by size:
1941 287 58
Bpu10I CC'TnA_GC
Cuts at: 0 1529 1886 2286
Size: 1529 357 400
Fragments arranged by size:
1529 400 357
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 876 2286
Size: 876 1410
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 696 726 2286
Size: 696 30 1560
Fragments arranged by size:
1560 696 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 531 717 1546 2286
Size: 531 186 829 740
Fragments arranged by size:
829 740 531 186
BseYI C'CCAG_C
Cuts at: 0 1434 2286
Size: 1434 852
BsiHKAI G_wGCw'C
Cuts at: 0 1887 2286
Size: 1887 399
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1797 2286
Size: 1797 489
Bsp1286I G_dGCh'C
Cuts at: 0 1051 1209 1560 1887 2286
Size: 1051 158 351 327 399
Fragments arranged by size:
1051 399 351 327 158
BspHI T'CATG_A
Cuts at: 0 1388 1637 2286
Size: 1388 249 649
Fragments arranged by size:
1388 649 249
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1163 2286
Size: 1163 1123
BsrDI GCAATG_nn'
Cuts at: 0 2025 2286
Size: 2025 261
BsrFI r'CCGG_y
Cuts at: 0 1175 2286
Size: 1175 1111
BstEII G'GTnAC_C
Cuts at: 0 279 2286
Size: 279 2007
BstYI r'GATC_y
Cuts at: 0 1535 2286
Size: 1535 751
BtgI C'CryG_G
Cuts at: 0 205 2286
Size: 205 2081
BtsI GCAGTG_nn'
Cuts at: 0 1783 2286
Size: 1783 503
EarI CTCTTCn'nnn_
Cuts at: 0 1092 1492 1696 2286
Size: 1092 400 204 590
Fragments arranged by size:
1092 590 400 204
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1023 1270 1677 2286
Size: 1023 247 407 609
Fragments arranged by size:
1023 609 407 247
EcoO109I rG'GnC_Cy
Cuts at: 0 887 1205 2286
Size: 887 318 1081
Fragments arranged by size:
1081 887 318
EcoRI G'AATT_C
Cuts at: 0 1290 2286
Size: 1290 996
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 162 2110 2286
Size: 162 1948 176
Fragments arranged by size:
1948 176 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 696 728 808 840 1162 1194
Size: 10 32 654 32 80 32 322 32
Cuts at: 1194 1527 1559 2166 2198 2230 2286
Size: 333 32 607 32 32 56
Fragments arranged by size:
654 607 333 322 80 56 32 32
32 32 32 32 32 10
HincII GTy'rAC
Cuts at: 0 159 176 715 2286
Size: 159 17 539 1571
Fragments arranged by size:
1571 539 159 17
KpnI G_GTAC'C
Cuts at: 0 36 2286
Size: 36 2250
MfeI C'AATT_G
Cuts at: 0 264 338 2286
Size: 264 74 1948
Fragments arranged by size:
1948 264 74
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1349 2286
Size: 1349 937
MslI CAynn'nnrTG
Cuts at: 0 1387 2286
Size: 1387 899
MspA1I CmG'CkG
Cuts at: 0 1528 2286
Size: 1528 758
NcoI C'CATG_G
Cuts at: 0 205 2286
Size: 205 2081
NspI r_CATG'y
Cuts at: 0 1092 1143 2286
Size: 1092 51 1143
Fragments arranged by size:
1143 1092 51
PciI A'CATG_T
Cuts at: 0 1088 1139 2286
Size: 1088 51 1147
Fragments arranged by size:
1147 1088 51
PsiI TTA'TAA
Cuts at: 0 1928 2239 2286
Size: 1928 311 47
Fragments arranged by size:
1928 311 47
PspOMI G'GGCC_C
Cuts at: 0 1205 2286
Size: 1205 1081
PvuII CAG'CTG
Cuts at: 0 1528 2286
Size: 1528 758
SalI G'TCGA_C
Cuts at: 0 713 2286
Size: 713 1573
SapI GCTCTTCn'nnn_
Cuts at: 0 1492 2286
Size: 1492 794
ScaI AGT'ACT
Cuts at: 0 383 2286
Size: 383 1903
SmlI C'TyrA_G
Cuts at: 0 891 1750 2059 2286
Size: 891 859 309 227
Fragments arranged by size:
891 859 309 227
SspI AAT'ATT
Cuts at: 0 1767 2030 2286
Size: 1767 263 256
Fragments arranged by size:
1767 263 256
StyI C'CwwG_G
Cuts at: 0 205 1896 2286
Size: 205 1691 390
Fragments arranged by size:
1691 390 205
TatI w'GTAC_w
Cuts at: 0 381 2286
Size: 381 1905
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1224 1256 2286
Size: 1224 32 1030
Fragments arranged by size:
1224 1030 32
Tth111I GACn'n_nGTC
Cuts at: 0 710 2286
Size: 710 1576
XbaI T'CTAG_A
Cuts at: 0 1051 2286
Size: 1051 1235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 43 2286
Size: 43 2243
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AlwNI ApaI ApoI
AseI BanI BanII BbvCI BciVI BglII Bme1580I BmrI
Bpu10I BpuEI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrFI BstEII BstYI BtgI BtsI
EarI Eco57MI EcoO109I EcoRI FalI Hin4I HincII KpnI
MfeI MmeI MslI MspA1I NcoI NspI PciI PsiI
PspOMI PvuII SalI SapI ScaI SmlI SspI StyI
TatI TstI Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AhdI AleI AlfI
AloI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI
BbeI BbsI BcgI BclI BfrBI BglI BlpI BmgBI
BmtI BplI BpmI BsaI BsaAI BsaBI BsaHI BsaWI
BsgI BsiEI BsiWI BsmI BspEI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspXI PsrI PstI PvuI
RsrII SacI SacII SanDI SbfI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI TaqII XhoI XmaI XmnI ZraI