Restriction map for
pFN21AB6251
(Linear) (Six-base) MAPSORT of: KIBB6251.seq Check: 615 from: 1 to: 2280
KIBB6251.orf
With 182 enzymes: SgfI *
July 3, 2012 16:17 ..
Acc65I G'GTAC_C
Cuts at: 0 940 2280
Size: 940 1340
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 1397 2280
Size: 707 690 883
Fragments arranged by size:
883 707 690
AflII C'TTAA_G
Cuts at: 0 514 2280
Size: 514 1766
AhdI GACnn_n'nnGTC
Cuts at: 0 1580 2280
Size: 1580 700
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1177 1209 1265 1297 2280
Size: 1177 32 56 32 983
Fragments arranged by size:
1177 983 56 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1276 1606 2280
Size: 1276 330 674
Fragments arranged by size:
1276 674 330
ApoI r'AATT_y
Cuts at: 0 1844 1900 2280
Size: 1844 56 380
Fragments arranged by size:
1844 380 56
BanI G'GyrC_C
Cuts at: 0 788 940 1525 2280
Size: 788 152 585 755
Fragments arranged by size:
788 755 585 152
BanII G_rGCy'C
Cuts at: 0 713 2280
Size: 713 1567
BbvCI CC'TCA_GC
Cuts at: 0 1103 2280
Size: 1103 1177
BciVI GTATCCnnnnn_n'
Cuts at: 0 798 1905 2280
Size: 798 1107 375
Fragments arranged by size:
1107 798 375
BclI T'GATC_A
Cuts at: 0 180 243 2061 2280
Size: 180 63 1818 219
Fragments arranged by size:
1818 219 180 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 744 1098 2280
Size: 744 354 1182
Fragments arranged by size:
1182 744 354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1298 1505 1793 2280
Size: 1298 207 288 487
Fragments arranged by size:
1298 487 288 207
Bpu10I CC'TnA_GC
Cuts at: 0 1103 1802 2280
Size: 1103 699 478
Fragments arranged by size:
1103 699 478
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 1533 2280
Size: 688 845 747
Fragments arranged by size:
845 747 688
BsaHI Gr'CG_yC
Cuts at: 0 1814 2280
Size: 1814 466
BsaWI w'CCGG_w
Cuts at: 0 713 2280
Size: 713 1567
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1503 1533 2280
Size: 1503 30 747
Fragments arranged by size:
1503 747 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1330 1708 2280
Size: 1330 378 572
Fragments arranged by size:
1330 572 378
BsiHKAI G_wGCw'C
Cuts at: 0 7 713 2280
Size: 7 706 1567
Fragments arranged by size:
1567 706 7
Bsp1286I G_dGCh'C
Cuts at: 0 7 713 2280
Size: 7 706 1567
Fragments arranged by size:
1567 706 7
BspEI T'CCGG_A
Cuts at: 0 713 2280
Size: 713 1567
BspHI T'CATG_A
Cuts at: 0 864 2280
Size: 864 1416
BsrBI CCG'CTC
Cuts at: 0 947 2280
Size: 947 1333
BsrDI GCAATG_nn'
Cuts at: 0 735 964 1378 2280
Size: 735 229 414 902
Fragments arranged by size:
902 735 414 229
BsrFI r'CCGG_y
Cuts at: 0 611 2280
Size: 611 1669
BstYI r'GATC_y
Cuts at: 0 1092 1273 2172 2280
Size: 1092 181 899 108
Fragments arranged by size:
1092 899 181 108
Bsu36I CC'TnA_GG
Cuts at: 0 1045 2280
Size: 1045 1235
BtgI C'CryG_G
Cuts at: 0 1370 2280
Size: 1370 910
BtsI GCAGTG_nn'
Cuts at: 0 93 1696 2280
Size: 93 1603 584
Fragments arranged by size:
1603 584 93
DraIII CAC_nnn'GTG
Cuts at: 0 907 2280
Size: 907 1373
EarI CTCTTCn'nnn_
Cuts at: 0 8 630 1365 2280
Size: 8 622 735 915
Fragments arranged by size:
915 735 622 8
EcoICRI GAG'CTC
Cuts at: 0 711 2280
Size: 711 1569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 1298 1397 1505 1793 2280
Size: 707 591 99 108 288 487
Fragments arranged by size:
707 591 487 288 108 99
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 788 2280
Size: 788 1492
HaeII r_GCGC'y
Cuts at: 0 757 2280
Size: 757 1523
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 48 80 340 372 1252 1284 1566 1598
Size: 48 32 260 32 880 32 282 32
Cuts at: 1598 1771 1803 2128 2160 2280
Size: 173 32 325 32 120
Fragments arranged by size:
880 325 282 260 173 120 48 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 872 1437 2280
Size: 872 565 843
Fragments arranged by size:
872 843 565
KpnI G_GTAC'C
Cuts at: 0 944 2280
Size: 944 1336
MfeI C'AATT_G
Cuts at: 0 2069 2280
Size: 2069 211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2208 2280
Size: 2208 72
MslI CAynn'nnrTG
Cuts at: 0 728 1131 1184 2280
Size: 728 403 53 1096
Fragments arranged by size:
1096 728 403 53
MspA1I CmG'CkG
Cuts at: 0 922 1603 2280
Size: 922 681 677
Fragments arranged by size:
922 681 677
NcoI C'CATG_G
Cuts at: 0 1370 2280
Size: 1370 910
NspI r_CATG'y
Cuts at: 0 651 1318 2280
Size: 651 667 962
Fragments arranged by size:
962 667 651
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1107 1139 2280
Size: 1107 32 1141
Fragments arranged by size:
1141 1107 32
PvuII CAG'CTG
Cuts at: 0 922 1603 2280
Size: 922 681 677
Fragments arranged by size:
922 681 677
SacI G_AGCT'C
Cuts at: 0 713 2280
Size: 713 1567
SfcI C'TryA_G
Cuts at: 0 539 585 593 1471 1504 2045 2280
Size: 539 46 8 878 33 541 235
Fragments arranged by size:
878 541 539 235 46 33 8
SmlI C'TyrA_G
Cuts at: 0 514 703 1548 2280
Size: 514 189 845 732
Fragments arranged by size:
845 732 514 189
SspI AAT'ATT
Cuts at: 0 501 2280
Size: 501 1779
StuI AGG'CCT
Cuts at: 0 1559 2280
Size: 1559 721
StyI C'CwwG_G
Cuts at: 0 362 470 1019 1370 1554 2222 2280
Size: 362 108 549 351 184 668 58
Fragments arranged by size:
668 549 362 351 184 108 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1417 2280
Size: 1417 863
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 984 1016 1503 1535 2280
Size: 984 32 487 32 745
Fragments arranged by size:
984 745 487 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 895 2280
Size: 895 1385
XmnI GAAnn'nnTTC
Cuts at: 0 860 2280
Size: 860 1420
Enzymes that do cut:
Acc65I AcuI AflII AhdI AloI AlwNI ApoI BanI
BanII BbvCI BciVI BclI BmrI BpmI Bpu10I BpuEI
BsaHI BsaWI BsaXI BsgI BsiHKAI Bsp1286I BspEI BspHI
BsrBI BsrDI BsrFI BstYI Bsu36I BtgI BtsI DraIII
EarI EcoICRI Eco57MI FalI HaeII Hin4I HincII KpnI
MfeI MmeI MslI MspA1I NcoI NspI PsrI PvuII
SacI SfcI SmlI SspI StuI StyI TaqII TstI
Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflIII AgeI AleI
AlfI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbsI BcgI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmtI BplI BsaI BsaAI BsaBI
BseRI BseYI BsiEI BsiWI BsmI BsmBI BspMI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DraI DrdI EaeI EagI EciI EcoNI
EcoO109I EcoRI EcoRV FseI FspI FspAI HindIII HpaI
KasI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TatI XbaI XcmI XhoI
XmaI ZraI