Restriction map for
pFN21AB6244
(Linear) (Six-base) MAPSORT of: KIBB6244.seq Check: 3800 from: 1 to: 2040
KIBB6244.orf
With 182 enzymes: SgfI *
July 3, 2012 16:16 ..
AccI GT'mk_AC
Cuts at: 0 689 2040
Size: 689 1351
Acc65I G'GTAC_C
Cuts at: 0 861 2040
Size: 861 1179
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 1324 2040
Size: 613 711 716
Fragments arranged by size:
716 711 613
AflIII A'CryG_T
Cuts at: 0 533 2040
Size: 533 1507
AleI CACnn'nnGTG
Cuts at: 0 715 2040
Size: 715 1325
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 639 1543 2040
Size: 639 904 497
Fragments arranged by size:
904 639 497
AlwNI CAG_nnn'CTG
Cuts at: 0 1707 2040
Size: 1707 333
ApoI r'AATT_y
Cuts at: 0 3 142 754 892 1825 2040
Size: 3 139 612 138 933 215
Fragments arranged by size:
933 612 215 139 138 3
AvaI C'yCGr_G
Cuts at: 0 1898 1961 2040
Size: 1898 63 79
Fragments arranged by size:
1898 79 63
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1609 1642 2040
Size: 1609 33 398
Fragments arranged by size:
1609 398 33
BanI G'GyrC_C
Cuts at: 0 432 728 861 1519 2040
Size: 432 296 133 658 521
Fragments arranged by size:
658 521 432 296 133
BanII G_rGCy'C
Cuts at: 0 347 1888 2040
Size: 347 1541 152
Fragments arranged by size:
1541 347 152
BbeI G_GCGC'C
Cuts at: 0 436 2040
Size: 436 1604
BbsI GAAGACnn'nnnn_
Cuts at: 0 852 2040
Size: 852 1188
BbvCI CC'TCA_GC
Cuts at: 0 1283 1451 2040
Size: 1283 168 589
Fragments arranged by size:
1283 589 168
BciVI GTATCCnnnnn_n'
Cuts at: 0 295 1162 1637 2040
Size: 295 867 475 403
Fragments arranged by size:
867 475 403 295
BglI GCCn_nnn'nGGC
Cuts at: 0 565 2040
Size: 565 1475
BlpI GC'TnA_GC
Cuts at: 0 701 745 2040
Size: 701 44 1295
Fragments arranged by size:
1295 701 44
Bme1580I G_kGCm'C
Cuts at: 0 733 1522 2040
Size: 733 789 518
Fragments arranged by size:
789 733 518
BmgBI CAC'GTC
Cuts at: 0 534 708 2040
Size: 534 174 1332
Fragments arranged by size:
1332 534 174
BmrI ACTGGGnnnn_n'
Cuts at: 0 813 2040
Size: 813 1227
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1752 2040
Size: 1752 288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1559 1872 2040
Size: 1559 313 168
Fragments arranged by size:
1559 313 168
Bpu10I CC'TnA_GC
Cuts at: 0 1283 1451 1769 2040
Size: 1283 168 318 271
Fragments arranged by size:
1283 318 271 168
BsaI GGTCTCn'nnnn_
Cuts at: 0 1888 2040
Size: 1888 152
BsaHI Gr'CG_yC
Cuts at: 0 433 2040
Size: 433 1607
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 639 669 1813 1843 2040
Size: 639 30 1144 30 197
Fragments arranged by size:
1144 639 197 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 372 647 1821 2040
Size: 372 275 1174 219
Fragments arranged by size:
1174 372 275 219
BseYI C'CCAG_C
Cuts at: 0 75 454 2040
Size: 75 379 1586
Fragments arranged by size:
1586 379 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1593 2040
Size: 1593 447
BsiHKAI G_wGCw'C
Cuts at: 0 130 556 707 2040
Size: 130 426 151 1333
Fragments arranged by size:
1333 426 151 130
Bsp1286I G_dGCh'C
Cuts at: 0 130 347 556 707 733 1522 1888 2040
Size: 130 217 209 151 26 789 366 152
Fragments arranged by size:
789 366 217 209 152 151 130 26
BsrFI r'CCGG_y
Cuts at: 0 1811 2040
Size: 1811 229
BsrGI T'GTAC_A
Cuts at: 0 908 2040
Size: 908 1132
BstYI r'GATC_y
Cuts at: 0 831 1028 1180 2040
Size: 831 197 152 860
Fragments arranged by size:
860 831 197 152
Bsu36I CC'TnA_GG
Cuts at: 0 659 2040
Size: 659 1381
BtgI C'CryG_G
Cuts at: 0 1739 2040
Size: 1739 301
BtsI GCAGTG_nn'
Cuts at: 0 1605 1869 2040
Size: 1605 264 171
Fragments arranged by size:
1605 264 171
DraIII CAC_nnn'GTG
Cuts at: 0 22 2040
Size: 22 2018
EaeI y'GGCC_r
Cuts at: 0 322 566 2040
Size: 322 244 1474
Fragments arranged by size:
1474 322 244
EarI CTCTTCn'nnn_
Cuts at: 0 1041 2040
Size: 1041 999
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1075 1927 2040
Size: 1075 852 113
Fragments arranged by size:
1075 852 113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 1324 1559 1872 2040
Size: 613 711 235 313 168
Fragments arranged by size:
711 613 313 235 168
EcoRI G'AATT_C
Cuts at: 0 142 754 892 1825 2040
Size: 142 612 138 933 215
Fragments arranged by size:
933 612 215 142 138
HaeII r_GCGC'y
Cuts at: 0 436 1128 2040
Size: 436 692 912
Fragments arranged by size:
912 692 436
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 223 255 460 492 639 656 671 688
Size: 223 32 205 32 147 17 15 17
Cuts at: 688 762 794 1772 1804 1919 1951 2040
Size: 74 32 978 32 115 32 89
Fragments arranged by size:
978 223 205 147 115 89 74 32
32 32 32 32 17 17 15
KasI G'GCGC_C
Cuts at: 0 432 2040
Size: 432 1608
KpnI G_GTAC'C
Cuts at: 0 865 2040
Size: 865 1175
MfeI C'AATT_G
Cuts at: 0 1292 2040
Size: 1292 748
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 918 2040
Size: 524 394 1122
Fragments arranged by size:
1122 524 394
MscI TGG'CCA
Cuts at: 0 324 2040
Size: 324 1716
MslI CAynn'nnrTG
Cuts at: 0 715 1510 1525 1531 2040
Size: 715 795 15 6 509
Fragments arranged by size:
795 715 509 15 6
NarI GG'CG_CC
Cuts at: 0 433 2040
Size: 433 1607
NcoI C'CATG_G
Cuts at: 0 1739 2040
Size: 1739 301
NdeI CA'TA_TG
Cuts at: 0 1374 2040
Size: 1374 666
NspI r_CATG'y
Cuts at: 0 294 626 1530 1786 2040
Size: 294 332 904 256 254
Fragments arranged by size:
904 332 294 256 254
PasI CC'CwG_GG
Cuts at: 0 1701 2040
Size: 1701 339
PflMI CCAn_nnn'nTGG
Cuts at: 0 1537 2040
Size: 1537 503
PfoI T'CCnGG_A
Cuts at: 0 1802 2040
Size: 1802 238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 367 399 2040
Size: 367 32 1641
Fragments arranged by size:
1641 367 32
ScaI AGT'ACT
Cuts at: 0 37 2040
Size: 37 2003
SfcI C'TryA_G
Cuts at: 0 31 1871 2040
Size: 31 1840 169
Fragments arranged by size:
1840 169 31
SfoI GGC'GCC
Cuts at: 0 434 2040
Size: 434 1606
SmaI CCC'GGG
Cuts at: 0 1900 2040
Size: 1900 140
SphI G_CATG'C
Cuts at: 0 294 2040
Size: 294 1746
StyI C'CwwG_G
Cuts at: 0 1739 2040
Size: 1739 301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1537 1579 2040
Size: 1537 42 461
Fragments arranged by size:
1537 461 42
TatI w'GTAC_w
Cuts at: 0 35 329 908 1099 2040
Size: 35 294 579 191 941
Fragments arranged by size:
941 579 294 191 35
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 476 508 1136 1168 1813 1845 2040
Size: 476 32 628 32 645 32 195
Fragments arranged by size:
645 628 476 195 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1746 2040
Size: 1746 294
XmaI C'CCGG_G
Cuts at: 0 1898 2040
Size: 1898 142
XmnI GAAnn'nnTTC
Cuts at: 0 1978 2040
Size: 1978 62
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI AlfI AlwNI ApoI
AvaI BaeI BanI BanII BbeI BbsI BbvCI BciVI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BsrFI BsrGI BstYI Bsu36I BtgI BtsI DraIII EaeI
EarI EciI Eco57MI EcoRI HaeII Hin4I KasI KpnI
MfeI MmeI MscI MslI NarI NcoI NdeI NspI
PasI PflMI PfoI PpiI ScaI SfcI SfoI SmaI
SphI StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BamHI BcgI
BclI BfrBI BglII BmtI BpuEI BsaAI BsaBI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI MluI MspA1I NaeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI
Tth111I XbaI XhoI ZraI