Restriction map for
pFN21AB6221
(Linear) (Six-base) MAPSORT of: KIBB6221.seq Check: 5534 from: 1 to: 2010
KIBB6221.orf
With 182 enzymes: SgfI *
March 4, 2011 18:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1733 2010
Size: 1733 277
Acc65I G'GTAC_C
Cuts at: 0 816 2010
Size: 816 1194
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 463 812 1063 1084 1385 1513 2010
Size: 413 50 349 251 21 301 128 497
Fragments arranged by size:
497 413 349 301 251 128 50 21
AlwNI CAG_nnn'CTG
Cuts at: 0 121 2010
Size: 121 1889
ApoI r'AATT_y
Cuts at: 0 509 519 1554 2010
Size: 509 10 1035 456
Fragments arranged by size:
1035 509 456 10
AvaI C'yCGr_G
Cuts at: 0 48 2010
Size: 48 1962
AvrII C'CTAG_G
Cuts at: 0 469 2010
Size: 469 1541
BamHI G'GATC_C
Cuts at: 0 1871 2010
Size: 1871 139
BanI G'GyrC_C
Cuts at: 0 25 198 816 1247 2010
Size: 25 173 618 431 763
Fragments arranged by size:
763 618 431 173 25
BanII G_rGCy'C
Cuts at: 0 1231 1552 2010
Size: 1231 321 458
Fragments arranged by size:
1231 458 321
BbsI GAAGACnn'nnnn_
Cuts at: 0 362 2010
Size: 362 1648
BbvCI CC'TCA_GC
Cuts at: 0 269 2010
Size: 269 1741
BclI T'GATC_A
Cuts at: 0 1323 1811 2010
Size: 1323 488 199
Fragments arranged by size:
1323 488 199
BglI GCCn_nnn'nGGC
Cuts at: 0 1969 2010
Size: 1969 41
BlpI GC'TnA_GC
Cuts at: 0 755 2010
Size: 755 1255
BmrI ACTGGGnnnn_n'
Cuts at: 0 835 2010
Size: 835 1175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 570 758 2010
Size: 266 304 188 1252
Fragments arranged by size:
1252 304 266 188
Bpu10I CC'TnA_GC
Cuts at: 0 269 919 2010
Size: 269 650 1091
Fragments arranged by size:
1091 650 269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 2010
Size: 276 1734
BsaAI yAC'GTr
Cuts at: 0 804 2010
Size: 804 1206
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 279 302 370 2010
Size: 279 23 68 1640
Fragments arranged by size:
1640 279 68 23
BseYI C'CCAG_C
Cuts at: 0 13 775 2010
Size: 13 762 1235
Fragments arranged by size:
1235 762 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 2010
Size: 659 1351
BsiHKAI G_wGCw'C
Cuts at: 0 1539 2010
Size: 1539 471
BsmBI CGTCTCn'nnnn_
Cuts at: 0 145 314 2010
Size: 145 169 1696
Fragments arranged by size:
1696 169 145
Bsp1286I G_dGCh'C
Cuts at: 0 1231 1539 1552 2010
Size: 1231 308 13 458
Fragments arranged by size:
1231 458 308 13
BspHI T'CATG_A
Cuts at: 0 1440 2010
Size: 1440 570
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 681 1733 2010
Size: 681 1052 277
Fragments arranged by size:
1052 681 277
BsrDI GCAATG_nn'
Cuts at: 0 1372 2010
Size: 1372 638
BsrFI r'CCGG_y
Cuts at: 0 9 22 2010
Size: 9 13 1988
Fragments arranged by size:
1988 13 9
BsrGI T'GTAC_A
Cuts at: 0 241 2010
Size: 241 1769
BstBI TT'CG_AA
Cuts at: 0 1713 2010
Size: 1713 297
BstYI r'GATC_y
Cuts at: 0 1871 2010
Size: 1871 139
Bsu36I CC'TnA_GG
Cuts at: 0 353 2010
Size: 353 1657
BtgI C'CryG_G
Cuts at: 0 29 2010
Size: 29 1981
DraIII CAC_nnn'GTG
Cuts at: 0 807 2010
Size: 807 1203
EarI CTCTTCn'nnn_
Cuts at: 0 432 1122 2010
Size: 432 690 888
Fragments arranged by size:
888 690 432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 413 463 570 758 812 1063 1084
Size: 266 147 50 107 188 54 251 21
Cuts at: 1084 1385 1513 2010
Size: 301 128 497
Fragments arranged by size:
497 301 266 251 188 147 128 107
54 50 21
EcoRI G'AATT_C
Cuts at: 0 519 2010
Size: 519 1491
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 930 962 1491 1523 1836 1868 2010
Size: 930 32 529 32 313 32 142
Fragments arranged by size:
930 529 313 142 32 32 32
KpnI G_GTAC'C
Cuts at: 0 820 2010
Size: 820 1190
MfeI C'AATT_G
Cuts at: 0 133 1775 2010
Size: 133 1642 235
Fragments arranged by size:
1642 235 133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 1172 2010
Size: 341 831 838
Fragments arranged by size:
838 831 341
MslI CAynn'nnrTG
Cuts at: 0 1466 2010
Size: 1466 544
MspA1I CmG'CkG
Cuts at: 0 433 1074 1563 2010
Size: 433 641 489 447
Fragments arranged by size:
641 489 447 433
NaeI GCC'GGC
Cuts at: 0 11 24 2010
Size: 11 13 1986
Fragments arranged by size:
1986 13 11
NcoI C'CATG_G
Cuts at: 0 29 2010
Size: 29 1981
NgoMIV G'CCGG_C
Cuts at: 0 9 22 2010
Size: 9 13 1988
Fragments arranged by size:
1988 13 9
NspI r_CATG'y
Cuts at: 0 349 1191 2010
Size: 349 842 819
Fragments arranged by size:
842 819 349
PflMI CCAn_nnn'nTGG
Cuts at: 0 847 2010
Size: 847 1163
PfoI T'CCnGG_A
Cuts at: 0 1204 2010
Size: 1204 806
PmlI CAC'GTG
Cuts at: 0 804 2010
Size: 804 1206
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1442 1474 2010
Size: 1442 32 536
Fragments arranged by size:
1442 536 32
PsiI TTA'TAA
Cuts at: 0 1162 2010
Size: 1162 848
PvuII CAG'CTG
Cuts at: 0 1074 1563 2010
Size: 1074 489 447
Fragments arranged by size:
1074 489 447
SfcI C'TryA_G
Cuts at: 0 1314 2010
Size: 1314 696
SmaI CCC'GGG
Cuts at: 0 50 2010
Size: 50 1960
SmlI C'TyrA_G
Cuts at: 0 291 2010
Size: 291 1719
SpeI A'CTAG_T
Cuts at: 0 1591 2010
Size: 1591 419
StyI C'CwwG_G
Cuts at: 0 29 202 469 2010
Size: 29 173 267 1541
Fragments arranged by size:
1541 267 173 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 305 582 693 1659 1759 2010
Size: 305 277 111 966 100 251
Fragments arranged by size:
966 305 277 251 111 100
TatI w'GTAC_w
Cuts at: 0 241 2010
Size: 241 1769
Tth111I GACn'n_nGTC
Cuts at: 0 1485 2010
Size: 1485 525
XmaI C'CCGG_G
Cuts at: 0 48 2010
Size: 48 1962
XmnI GAAnn'nnTTC
Cuts at: 0 855 2010
Size: 855 1155
Enzymes that do cut:
AarI Acc65I AcuI AlwNI ApoI AvaI AvrII BamHI
BanI BanII BbsI BbvCI BclI BglI BlpI BmrI
BpmI Bpu10I BpuEI BsaAI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstBI
BstYI Bsu36I BtgI DraIII EarI Eco57MI EcoRI Hin4I
KpnI MfeI MmeI MslI MspA1I NaeI NcoI NgoMIV
NspI PflMI PfoI PmlI PpiI PsiI PvuII SfcI
SmaI SmlI SpeI StyI TaqII TatI Tth111I XmaI
XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
BaeI BbeI BcgI BciVI BfrBI BglII Bme1580I BmgBI
BmtI BplI BsaI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiWI BsmI BspEI BsrBI BssHII BssSI BstAPI BstEII
BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
MluI MscI NarI NdeI NheI NotI NruI NsiI
PacI PasI PciI PmeI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SphI SrfI SspI StuI SwaI TstI XbaI
XcmI XhoI ZraI