Restriction map for
pFN21AB6220
(Linear) (Six-base) MAPSORT of: KIBB6220.seq Check: 8320 from: 1 to: 2202
KIBB6220.orf
With 182 enzymes: SgfI *
July 3, 2012 16:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 534 1202 2202
Size: 534 668 1000
Fragments arranged by size:
1000 668 534
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 1161 1800 2202
Size: 140 1021 639 402
Fragments arranged by size:
1021 639 402 140
AfeI AGC'GCT
Cuts at: 0 1354 1561 1657 1813 2202
Size: 1354 207 96 156 389
Fragments arranged by size:
1354 389 207 156 96
AflIII A'CryG_T
Cuts at: 0 1837 2202
Size: 1837 365
AleI CACnn'nnGTG
Cuts at: 0 1136 1880 2027 2202
Size: 1136 744 147 175
Fragments arranged by size:
1136 744 175 147
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1126 2202
Size: 1126 1076
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1216 1248 1301 1333 2202
Size: 1216 32 53 32 869
Fragments arranged by size:
1216 869 53 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 118 216 249 276 493 2097 2202
Size: 118 98 33 27 217 1604 105
Fragments arranged by size:
1604 217 118 105 98 33 27
ApaI G_GGCC'C
Cuts at: 0 173 500 735 982 1402 2202
Size: 173 327 235 247 420 800
Fragments arranged by size:
800 420 327 247 235 173
ApaLI G'TGCA_C
Cuts at: 0 1297 1600 2202
Size: 1297 303 602
Fragments arranged by size:
1297 602 303
AscI GG'CGCG_CC
Cuts at: 0 1488 2202
Size: 1488 714
AvaI C'yCGr_G
Cuts at: 0 334 1395 1435 1798 1966 2202
Size: 334 1061 40 363 168 236
Fragments arranged by size:
1061 363 334 236 168 40
AvrII C'CTAG_G
Cuts at: 0 511 2202
Size: 511 1691
BamHI G'GATC_C
Cuts at: 0 960 2202
Size: 960 1242
BanI G'GyrC_C
Cuts at: 0 1118 2202
Size: 1118 1084
BanII G_rGCy'C
Cuts at: 0 22 173 446 500 735 982 1402 2202
Size: 22 151 273 54 235 247 420 800
Fragments arranged by size:
800 420 273 247 235 151 54 22
BbvCI CC'TCA_GC
Cuts at: 0 1114 2202
Size: 1114 1088
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 325 359 2202
Size: 325 34 1843
Fragments arranged by size:
1843 325 34
BclI T'GATC_A
Cuts at: 0 989 2202
Size: 989 1213
BglI GCCn_nnn'nGGC
Cuts at: 0 866 2202
Size: 866 1336
BglII A'GATC_T
Cuts at: 0 674 2202
Size: 674 1528
BlpI GC'TnA_GC
Cuts at: 0 1409 2202
Size: 1409 793
Bme1580I G_kGCm'C
Cuts at: 0 173 500 735 982 1301 1402 1604 2202
Size: 173 327 235 247 319 101 202 598
Fragments arranged by size:
598 327 319 247 235 202 173 101
BmgBI CAC'GTC
Cuts at: 0 834 2202
Size: 834 1368
BmrI ACTGGGnnnn_n'
Cuts at: 0 544 704 1052 2202
Size: 544 160 348 1150
Fragments arranged by size:
1150 544 348 160
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 249 726 2202
Size: 249 477 1476
Fragments arranged by size:
1476 477 249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 286 432 470 607 1522 2202
Size: 271 15 146 38 137 915 680
Fragments arranged by size:
915 680 271 146 137 38 15
Bpu10I CC'TnA_GC
Cuts at: 0 1114 2202
Size: 1114 1088
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1813 2202
Size: 1813 389
BsaI GGTCTCn'nnnn_
Cuts at: 0 1914 2202
Size: 1914 288
BsaWI w'CCGG_w
Cuts at: 0 140 2202
Size: 140 2062
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 462 492 614 644 1216 1246 2202
Size: 462 30 122 30 572 30 956
Fragments arranged by size:
956 572 462 122 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 100 172 352 568 655 948 1237
Size: 97 3 72 180 216 87 293 289
Cuts at: 1237 2202
Size: 965
Fragments arranged by size:
965 293 289 216 180 97 87 72
3
BseYI C'CCAG_C
Cuts at: 0 15 188 751 922 1036 2006 2202
Size: 15 173 563 171 114 970 196
Fragments arranged by size:
970 563 196 173 171 114 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 331 430 1176 1479 2202
Size: 110 221 99 746 303 723
Fragments arranged by size:
746 723 303 221 110 99
BsiEI CG_ry'CG
Cuts at: 0 1068 1170 1231 1569 2202
Size: 1068 102 61 338 633
Fragments arranged by size:
1068 633 338 102 61
BsiHKAI G_wGCw'C
Cuts at: 0 36 725 1301 1508 1604 1928 2164 2202
Size: 36 689 576 207 96 324 236 38
Fragments arranged by size:
689 576 324 236 207 96 38 36
Bsp1286I G_dGCh'C
Cuts at: 0 22 36 173 446 500 725 735 982
Size: 22 14 137 273 54 225 10 247
Cuts at: 982 1301 1402 1508 1604 1928 2164 2202
Size: 319 101 106 96 324 236 38
Fragments arranged by size:
324 319 273 247 236 225 137 106
101 96 54 38 22 14 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 534 793 1118 1202 1896 2202
Size: 534 259 325 84 694 306
Fragments arranged by size:
694 534 325 306 259 84
BsrBI CCG'CTC
Cuts at: 0 1227 1574 1614 1698 2202
Size: 1227 347 40 84 504
Fragments arranged by size:
1227 504 347 84 40
BsrFI r'CCGG_y
Cuts at: 0 1592 1676 1858 2012 2202
Size: 1592 84 182 154 190
Fragments arranged by size:
1592 190 182 154 84
BssHII G'CGCG_C
Cuts at: 0 1271 1273 1488 2202
Size: 1271 2 215 714
Fragments arranged by size:
1271 714 215 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1163 1197 1466 2097 2202
Size: 1163 34 269 631 105
Fragments arranged by size:
1163 631 269 105 34
BstXI CCAn_nnnn'nTGG
Cuts at: 0 655 2140 2202
Size: 655 1485 62
Fragments arranged by size:
1485 655 62
BstYI r'GATC_y
Cuts at: 0 296 674 872 960 1127 2202
Size: 296 378 198 88 167 1075
Fragments arranged by size:
1075 378 296 198 167 88
Bsu36I CC'TnA_GG
Cuts at: 0 638 701 2202
Size: 638 63 1501
Fragments arranged by size:
1501 638 63
BtgI C'CryG_G
Cuts at: 0 950 1383 2202
Size: 950 433 819
Fragments arranged by size:
950 819 433
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1088 2202
Size: 1088 1114
BtsI GCAGTG_nn'
Cuts at: 0 283 1875 2202
Size: 283 1592 327
Fragments arranged by size:
1592 327 283
EaeI y'GGCC_r
Cuts at: 0 322 867 1065 1167 1228 1251 1335 1566
Size: 322 545 198 102 61 23 84 231
Cuts at: 1566 1638 1806 1974 2142 2202
Size: 72 168 168 168 60
Fragments arranged by size:
545 322 231 198 168 168 168 102
84 72 61 60 23
EagI C'GGCC_G
Cuts at: 0 1065 1167 1228 1566 2202
Size: 1065 102 61 338 636
Fragments arranged by size:
1065 636 338 102 61
EarI CTCTTCn'nnn_
Cuts at: 0 1277 2202
Size: 1277 925
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 395 1723 2059 2202
Size: 395 1328 336 143
Fragments arranged by size:
1328 395 336 143
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 271 286 432 470 607 1161 1522
Size: 140 131 15 146 38 137 554 361
Cuts at: 1522 1800 2202
Size: 278 402
Fragments arranged by size:
554 402 361 278 146 140 137 131
38 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 637 714 2202
Size: 637 77 1488
Fragments arranged by size:
1488 637 77
EcoO109I rG'GnC_Cy
Cuts at: 0 52 108 115 169 170 180 423 706
Size: 52 56 7 54 1 10 243 283
Cuts at: 706 731 732 843 945 979 1399 2202
Size: 25 1 111 102 34 420 803
Fragments arranged by size:
803 420 283 243 111 102 56 54
52 34 25 10 7 1 1
FspI TGC'GCA
Cuts at: 0 905 1267 2101 2202
Size: 905 362 834 101
Fragments arranged by size:
905 834 362 101
HaeII r_GCGC'y
Cuts at: 0 1008 1356 1409 1563 1659 1815 1827 2198
Size: 1008 348 53 154 96 156 12 371
Cuts at: 2198 2202
Size: 4
Fragments arranged by size:
1008 371 348 156 154 96 53 12
4
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 412 444 2202
Size: 412 32 1758
Fragments arranged by size:
1758 412 32
MluI A'CGCG_T
Cuts at: 0 1837 2202
Size: 1837 365
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 1685 2202
Size: 505 1180 517
Fragments arranged by size:
1180 517 505
MscI TGG'CCA
Cuts at: 0 869 1253 1808 2202
Size: 869 384 555 394
Fragments arranged by size:
869 555 394 384
MslI CAynn'nnrTG
Cuts at: 0 1136 1152 1880 2027 2202
Size: 1136 16 728 147 175
Fragments arranged by size:
1136 728 175 147 16
MspA1I CmG'CkG
Cuts at: 0 321 1001 1740 2076 2202
Size: 321 680 739 336 126
Fragments arranged by size:
739 680 336 321 126
NotI GC'GGCC_GC
Cuts at: 0 1167 2202
Size: 1167 1035
NspI r_CATG'y
Cuts at: 0 910 2048 2202
Size: 910 1138 154
Fragments arranged by size:
1138 910 154
PasI CC'CwG_GG
Cuts at: 0 728 769 839 1013 2202
Size: 728 41 70 174 1189
Fragments arranged by size:
1189 728 174 70 41
PflMI CCAn_nnn'nTGG
Cuts at: 0 493 1001 2202
Size: 493 508 1201
Fragments arranged by size:
1201 508 493
PfoI T'CCnGG_A
Cuts at: 0 584 2202
Size: 584 1618
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1216 1248 2202
Size: 1216 32 954
Fragments arranged by size:
1216 954 32
PpuMI rG'GwC_Cy
Cuts at: 0 108 423 706 843 945 2202
Size: 108 315 283 137 102 1257
Fragments arranged by size:
1257 315 283 137 108 102
PspOMI G'GGCC_C
Cuts at: 0 169 496 731 978 1398 2202
Size: 169 327 235 247 420 804
Fragments arranged by size:
804 420 327 247 235 169
PstI C_TGCA'G
Cuts at: 0 545 785 1370 1589 2202
Size: 545 240 585 219 613
Fragments arranged by size:
613 585 545 240 219
SanDI GG'GwC_CC
Cuts at: 0 108 706 2202
Size: 108 598 1496
Fragments arranged by size:
1496 598 108
SapI GCTCTTCn'nnn_
Cuts at: 0 1277 2202
Size: 1277 925
SbfI CC_TGCA'GG
Cuts at: 0 545 2202
Size: 545 1657
SfcI C'TryA_G
Cuts at: 0 541 781 1366 1585 2202
Size: 541 240 585 219 617
Fragments arranged by size:
617 585 541 240 219
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 866 2202
Size: 866 1336
SgrAI Cr'CCGG_yG
Cuts at: 0 1592 1676 1858 2012 2202
Size: 1592 84 182 154 190
Fragments arranged by size:
1592 190 182 154 84
SmaI CCC'GGG
Cuts at: 0 1397 2202
Size: 1397 805
SmlI C'TyrA_G
Cuts at: 0 1828 2202
Size: 1828 374
SphI G_CATG'C
Cuts at: 0 910 2048 2202
Size: 910 1138 154
Fragments arranged by size:
1138 910 154
StuI AGG'CCT
Cuts at: 0 6 1729 2065 2202
Size: 6 1723 336 137
Fragments arranged by size:
1723 336 137 6
StyI C'CwwG_G
Cuts at: 0 236 511 2202
Size: 236 275 1691
Fragments arranged by size:
1691 275 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 44 625 2020 2119 2202
Size: 44 581 1395 99 83
Fragments arranged by size:
1395 581 99 83 44
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 691 2202
Size: 691 1511
XmaI C'CCGG_G
Cuts at: 0 1395 2202
Size: 1395 807
XmnI GAAnn'nnTTC
Cuts at: 0 2122 2202
Size: 2122 80
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AloI AlwNI
ApaI ApaLI AscI AvaI AvrII BamHI BanI BanII
BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
FspI HaeII Hin4I MluI MmeI MscI MslI MspA1I
NotI NspI PasI PflMI PfoI PpiI PpuMI PspOMI
PstI SanDI SapI SbfI SfcI SfiI SgrAI SmaI
SmlI SphI StuI StyI TaqII XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI ApoI
AseI AsiSI BaeI BbeI BbsI BciVI BfrBI BmtI
BsaAI BsaBI BsaHI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssSI BstBI BstEII BstZ17I ClaI CspCI
DraI DraIII DrdI EcoICRI EcoRI EcoRV FalI FseI
FspAI HincII HindIII HpaI KasI KpnI MfeI NaeI
NarI NcoI NdeI NgoMIV NheI NruI NsiI PacI
PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI ScaI SexAI SfoI
SgfI SnaBI SpeI SrfI SspI SwaI TatI TstI
Tth111I XbaI XhoI ZraI