Restriction map for
pFN21AB6192
(Linear) (Six-base) MAPSORT of: KIBB6192.seq Check: 8205 from: 1 to: 2118
KIBB6192.orf
With 182 enzymes: SgfI *
December 27, 2011 16:29 ..
AccI GT'mk_AC
Cuts at: 0 134 2118
Size: 134 1984
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 797 962 1050 1134 1218 1386 1470
Size: 757 40 165 88 84 84 168 84
Cuts at: 1470 1554 2058 2118
Size: 84 504 60
Fragments arranged by size:
757 504 168 165 88 84 84 84
84 60 40
AflIII A'CryG_T
Cuts at: 0 350 427 2118
Size: 350 77 1691
Fragments arranged by size:
1691 350 77
AlwNI CAG_nnn'CTG
Cuts at: 0 719 2118
Size: 719 1399
ApaI G_GGCC'C
Cuts at: 0 55 264 2118
Size: 55 209 1854
Fragments arranged by size:
1854 209 55
ApoI r'AATT_y
Cuts at: 0 810 847 931 1016 1604 1732 1816 2118
Size: 810 37 84 85 588 128 84 302
Fragments arranged by size:
810 588 302 128 85 84 84 37
BamHI G'GATC_C
Cuts at: 0 305 2118
Size: 305 1813
BanI G'GyrC_C
Cuts at: 0 179 2015 2118
Size: 179 1836 103
Fragments arranged by size:
1836 179 103
BanII G_rGCy'C
Cuts at: 0 55 161 264 547 745 2118
Size: 55 106 103 283 198 1373
Fragments arranged by size:
1373 283 198 106 103 55
BbvCI CC'TCA_GC
Cuts at: 0 916 2118
Size: 916 1202
BglII A'GATC_T
Cuts at: 0 1999 2118
Size: 1999 119
BlpI GC'TnA_GC
Cuts at: 0 84 389 1340 2118
Size: 84 305 951 778
Fragments arranged by size:
951 778 305 84
Bme1580I G_kGCm'C
Cuts at: 0 55 264 2118
Size: 55 209 1854
Fragments arranged by size:
1854 209 55
BmrI ACTGGGnnnn_n'
Cuts at: 0 866 1538 2118
Size: 866 672 580
Fragments arranged by size:
866 672 580
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 764 2118
Size: 764 1354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1130 1284 1298 1466 1566 1620 1634
Size: 607 523 154 14 168 100 54 14
Cuts at: 1634 1704 1802 1872 1886 2118
Size: 70 98 70 14 232
Fragments arranged by size:
607 523 232 168 154 100 98 70
70 54 14 14 14
Bpu10I CC'TnA_GC
Cuts at: 0 916 2118
Size: 916 1202
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 2118
Size: 232 1886
BsaWI w'CCGG_w
Cuts at: 0 1360 2118
Size: 1360 758
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1312 1342 2118
Size: 1312 30 776
Fragments arranged by size:
1312 776 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 427 610 835 2118
Size: 163 264 183 225 1283
Fragments arranged by size:
1283 264 225 183 163
BseYI C'CCAG_C
Cuts at: 0 10 2118
Size: 10 2108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 732 1233 1401 1569 2118
Size: 732 501 168 168 549
Fragments arranged by size:
732 549 501 168 168
BsiHKAI G_wGCw'C
Cuts at: 0 161 491 547 745 2118
Size: 161 330 56 198 1373
Fragments arranged by size:
1373 330 198 161 56
BsmI GAATG_Cn'
Cuts at: 0 465 1300 1468 1888 2118
Size: 465 835 168 420 230
Fragments arranged by size:
835 465 420 230 168
Bsp1286I G_dGCh'C
Cuts at: 0 55 161 264 491 547 745 2118
Size: 55 106 103 227 56 198 1373
Fragments arranged by size:
1373 227 198 106 103 56 55
BsrDI GCAATG_nn'
Cuts at: 0 962 1725 2118
Size: 962 763 393
Fragments arranged by size:
962 763 393
BstEII G'GTnAC_C
Cuts at: 0 111 2118
Size: 111 2007
BstYI r'GATC_y
Cuts at: 0 305 318 1999 2118
Size: 305 13 1681 119
Fragments arranged by size:
1681 305 119 13
BstZ17I GTA'TAC
Cuts at: 0 135 2118
Size: 135 1983
BtsI GCAGTG_nn'
Cuts at: 0 1305 1389 1809 1893 2118
Size: 1305 84 420 84 225
Fragments arranged by size:
1305 420 225 84 84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 133 168 1656 1691 1707 1742 2118
Size: 133 35 1488 35 16 35 376
Fragments arranged by size:
1488 376 133 35 35 35 16
DraIII CAC_nnn'GTG
Cuts at: 0 195 2118
Size: 195 1923
EarI CTCTTCn'nnn_
Cuts at: 0 97 535 709 733 773 1137 1305 1389
Size: 97 438 174 24 40 364 168 84
Cuts at: 1389 2077 2118
Size: 688 41
Fragments arranged by size:
688 438 364 174 168 97 84 41
40 24
EcoICRI GAG'CTC
Cuts at: 0 159 545 743 2118
Size: 159 386 198 1375
Fragments arranged by size:
1375 386 198 159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 757 797 962 1050 1130 1134 1218
Size: 607 150 40 165 88 80 4 84
Cuts at: 1218 1284 1298 1386 1466 1470 1554 1566 1620
Size: 66 14 88 80 4 84 12 54
Cuts at: 1620 1634 1704 1802 1872 1886 2058 2118
Size: 14 70 98 70 14 172 60
Fragments arranged by size:
607 172 165 150 98 88 88 84
84 80 80 70 70 66 60 54
40 14 14 14 12 4 4
EcoO109I rG'GnC_Cy
Cuts at: 0 260 1958 2118
Size: 260 1698 160
Fragments arranged by size:
1698 260 160
EcoRI G'AATT_C
Cuts at: 0 1016 1604 2118
Size: 1016 588 514
Fragments arranged by size:
1016 588 514
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 811 843 1067 1099 2118
Size: 811 32 224 32 1019
Fragments arranged by size:
1019 811 224 32 32
HincII GTy'rAC
Cuts at: 0 639 2118
Size: 639 1479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1355 2118
Size: 1355 763
MslI CAynn'nnrTG
Cuts at: 0 792 2118
Size: 792 1326
MspA1I CmG'CkG
Cuts at: 0 88 611 2118
Size: 88 523 1507
Fragments arranged by size:
1507 523 88
NspI r_CATG'y
Cuts at: 0 354 431 440 797 971 2118
Size: 354 77 9 357 174 1147
Fragments arranged by size:
1147 357 354 174 77 9
PciI A'CATG_T
Cuts at: 0 350 427 2118
Size: 350 77 1691
Fragments arranged by size:
1691 350 77
PflMI CCAn_nnn'nTGG
Cuts at: 0 805 2118
Size: 805 1313
PsiI TTA'TAA
Cuts at: 0 1796 2118
Size: 1796 322
PspOMI G'GGCC_C
Cuts at: 0 51 260 2118
Size: 51 209 1858
Fragments arranged by size:
1858 209 51
PvuII CAG'CTG
Cuts at: 0 88 611 2118
Size: 88 523 1507
Fragments arranged by size:
1507 523 88
SacI G_AGCT'C
Cuts at: 0 161 547 745 2118
Size: 161 386 198 1373
Fragments arranged by size:
1373 386 198 161
SapI GCTCTTCn'nnn_
Cuts at: 0 97 535 733 1137 1305 2118
Size: 97 438 198 404 168 813
Fragments arranged by size:
813 438 404 198 168 97
SexAI A'CCwGG_T
Cuts at: 0 1922 2118
Size: 1922 196
SfcI C'TryA_G
Cuts at: 0 284 2118
Size: 284 1834
SmlI C'TyrA_G
Cuts at: 0 211 2118
Size: 211 1907
SspI AAT'ATT
Cuts at: 0 952 2118
Size: 952 1166
StuI AGG'CCT
Cuts at: 0 895 1119 1539 1707 1875 2118
Size: 895 224 420 168 168 243
Fragments arranged by size:
895 420 243 224 168 168
StyI C'CwwG_G
Cuts at: 0 834 2118
Size: 834 1284
TatI w'GTAC_w
Cuts at: 0 1521 2118
Size: 1521 597
XmnI GAAnn'nnTTC
Cuts at: 0 571 703 979 1063 1567 2071 2118
Size: 571 132 276 84 504 504 47
Fragments arranged by size:
571 504 504 276 132 84 47
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaI ApoI BamHI BanI
BanII BbvCI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BsrDI BstEII BstYI BstZ17I BtsI CspCI
DraIII EarI EcoICRI Eco57MI EcoO109I EcoRI Hin4I HincII
MmeI MslI MspA1I NspI PciI PflMI PsiI PspOMI
PvuII SacI SapI SexAI SfcI SmlI SspI StuI
StyI TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbsI BcgI BciVI BclI BfrBI
BglI BmgBI BmtI BsaI BsaAI BsaBI BsaHI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI Bsu36I BtgI BtgZI
ClaI DraI DrdI EaeI EagI EciI EcoNI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PfoI
PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PstI
PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI
ZraI