Restriction map for
pFN21AB6156
(Linear) (Six-base) MAPSORT of: KIBB6156.seq Check: 1891 from: 1 to: 1911
KIBB6156.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AccI GT'mk_AC
Cuts at: 0 6 1911
Size: 6 1905
Acc65I G'GTAC_C
Cuts at: 0 29 1911
Size: 29 1882
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 469 754 787 886 1911
Size: 33 436 285 33 99 1025
Fragments arranged by size:
1025 436 285 99 33 33
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1163 1911
Size: 1163 748
AlwNI CAG_nnn'CTG
Cuts at: 0 621 1401 1911
Size: 621 780 510
Fragments arranged by size:
780 621 510
ApoI r'AATT_y
Cuts at: 0 847 1176 1190 1911
Size: 847 329 14 721
Fragments arranged by size:
847 721 329 14
AvaI C'yCGr_G
Cuts at: 0 533 1911
Size: 533 1378
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 21 54 1911
Size: 21 33 1857
Fragments arranged by size:
1857 33 21
BamHI G'GATC_C
Cuts at: 0 1828 1911
Size: 1828 83
BanI G'GyrC_C
Cuts at: 0 29 1911
Size: 29 1882
BanII G_rGCy'C
Cuts at: 0 686 1789 1804 1911
Size: 686 1103 15 107
Fragments arranged by size:
1103 686 107 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 1373 1911
Size: 1373 538
BbvCI CC'TCA_GC
Cuts at: 0 1055 1100 1911
Size: 1055 45 811
Fragments arranged by size:
1055 811 45
BclI T'GATC_A
Cuts at: 0 741 1911
Size: 741 1170
BglII A'GATC_T
Cuts at: 0 1225 1253 1911
Size: 1225 28 658
Fragments arranged by size:
1225 658 28
Bme1580I G_kGCm'C
Cuts at: 0 1494 1911
Size: 1494 417
BmgBI CAC'GTC
Cuts at: 0 256 1911
Size: 256 1655
BmrI ACTGGGnnnn_n'
Cuts at: 0 1062 1911
Size: 1062 849
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 1360 1450 1477 1502 1601 1643 1703
Size: 622 738 90 27 25 99 42 60
Cuts at: 1703 1772 1826 1911
Size: 69 54 85
Fragments arranged by size:
738 622 99 90 85 69 60 54
42 27 25
Bpu10I CC'TnA_GC
Cuts at: 0 1055 1100 1911
Size: 1055 45 811
Fragments arranged by size:
1055 811 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1294 1911
Size: 1294 617
BsaWI w'CCGG_w
Cuts at: 0 797 1600 1660 1717 1897 1911
Size: 797 803 60 57 180 14
Fragments arranged by size:
803 797 180 60 57 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 134 260 1568 1911
Size: 134 126 1308 343
Fragments arranged by size:
1308 343 134 126
BseYI C'CCAG_C
Cuts at: 0 64 1911
Size: 64 1847
BsiEI CG_ry'CG
Cuts at: 0 1644 1782 1911
Size: 1644 138 129
Fragments arranged by size:
1644 138 129
BsiHKAI G_wGCw'C
Cuts at: 0 1519 1804 1911
Size: 1519 285 107
Fragments arranged by size:
1519 285 107
BsmI GAATG_Cn'
Cuts at: 0 1308 1911
Size: 1308 603
Bsp1286I G_dGCh'C
Cuts at: 0 686 1494 1519 1789 1804 1911
Size: 686 808 25 270 15 107
Fragments arranged by size:
808 686 270 107 25 15
BspEI T'CCGG_A
Cuts at: 0 1600 1660 1717 1897 1911
Size: 1600 60 57 180 14
Fragments arranged by size:
1600 180 60 57 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 639 1911
Size: 639 1272
BsrDI GCAATG_nn'
Cuts at: 0 1049 1911
Size: 1049 862
BstXI CCAn_nnnn'nTGG
Cuts at: 0 237 1911
Size: 237 1674
BstYI r'GATC_y
Cuts at: 0 515 1225 1253 1425 1828 1911
Size: 515 710 28 172 403 83
Fragments arranged by size:
710 515 403 172 83 28
BtgI C'CryG_G
Cuts at: 0 1644 1701 1911
Size: 1644 57 210
Fragments arranged by size:
1644 210 57
BtsI GCAGTG_nn'
Cuts at: 0 120 375 1145 1734 1911
Size: 120 255 770 589 177
Fragments arranged by size:
770 589 255 177 120
EaeI y'GGCC_r
Cuts at: 0 1641 1698 1779 1911
Size: 1641 57 81 132
Fragments arranged by size:
1641 132 81 57
EagI C'GGCC_G
Cuts at: 0 1641 1779 1911
Size: 1641 138 132
Fragments arranged by size:
1641 138 132
EarI CTCTTCn'nnn_
Cuts at: 0 730 1084 1127 1911
Size: 730 354 43 784
Fragments arranged by size:
784 730 354 43
EcoICRI GAG'CTC
Cuts at: 0 1802 1911
Size: 1802 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 469 622 754 787 886 1360 1450
Size: 33 436 153 132 33 99 474 90
Cuts at: 1450 1477 1502 1601 1643 1703 1772 1826 1911
Size: 27 25 99 42 60 69 54 85
Fragments arranged by size:
474 436 153 132 99 99 90 85
69 60 54 42 33 33 27 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1354 1456 1911
Size: 1354 102 455
Fragments arranged by size:
1354 455 102
EcoO109I rG'GnC_Cy
Cuts at: 0 1448 1911
Size: 1448 463
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 966 998 1093 1125 1217 1249 1911
Size: 966 32 95 32 92 32 662
Fragments arranged by size:
966 662 95 92 32 32 32
HincII GTy'rAC
Cuts at: 0 7 204 1911
Size: 7 197 1707
Fragments arranged by size:
1707 197 7
HpaI GTT'AAC
Cuts at: 0 204 1911
Size: 204 1707
KpnI G_GTAC'C
Cuts at: 0 33 1911
Size: 33 1878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 734 1283 1911
Size: 172 562 549 628
Fragments arranged by size:
628 562 549 172
MslI CAynn'nnrTG
Cuts at: 0 235 1911
Size: 235 1676
NcoI C'CATG_G
Cuts at: 0 1701 1911
Size: 1701 210
NspI r_CATG'y
Cuts at: 0 777 1911
Size: 777 1134
PfoI T'CCnGG_A
Cuts at: 0 599 1454 1911
Size: 599 855 457
Fragments arranged by size:
855 599 457
PpuMI rG'GwC_Cy
Cuts at: 0 1448 1911
Size: 1448 463
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1602 1634 1911
Size: 1602 32 277
Fragments arranged by size:
1602 277 32
PstI C_TGCA'G
Cuts at: 0 647 1142 1355 1911
Size: 647 495 213 556
Fragments arranged by size:
647 556 495 213
SacI G_AGCT'C
Cuts at: 0 1804 1911
Size: 1804 107
SalI G'TCGA_C
Cuts at: 0 5 1911
Size: 5 1906
SapI GCTCTTCn'nnn_
Cuts at: 0 1084 1127 1911
Size: 1084 43 784
Fragments arranged by size:
1084 784 43
SexAI A'CCwGG_T
Cuts at: 0 614 1911
Size: 614 1297
SfcI C'TryA_G
Cuts at: 0 643 1138 1351 1443 1737 1911
Size: 643 495 213 92 294 174
Fragments arranged by size:
643 495 294 213 174 92
SmlI C'TyrA_G
Cuts at: 0 533 1309 1911
Size: 533 776 602
Fragments arranged by size:
776 602 533
StyI C'CwwG_G
Cuts at: 0 207 890 1361 1701 1911
Size: 207 683 471 340 210
Fragments arranged by size:
683 471 340 210 207
Tth111I GACn'n_nGTC
Cuts at: 0 1240 1911
Size: 1240 671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1368 1419 1911
Size: 1368 51 492
Fragments arranged by size:
1368 492 51
XhoI C'TCGA_G
Cuts at: 0 533 1911
Size: 533 1378
Enzymes that do cut:
AccI Acc65I AcuI AlfI AlwNI ApoI AvaI BaeI
BamHI BanI BanII BbsI BbvCI BclI BglII Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI BstXI
BstYI BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I Hin4I HincII HpaI KpnI MmeI MslI
NcoI NspI PfoI PpuMI PsrI PstI SacI SalI
SapI SexAI SfcI SmlI StyI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BbeI BcgI BciVI BfrBI BglI BlpI BmtI BplI
BsaI BsaAI BsaBI BsaHI BsaXI BsgI BsiWI BsmBI
BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EciI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HindIII KasI MfeI MluI MscI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PvuI PvuII RsrII SacII SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
XbaI XmaI XmnI ZraI