Restriction map for
pFN21AB6147
(Linear) (Six-base) MAPSORT of: KIBB6147.seq Check: 4623 from: 1 to: 1788
KIBB6147.orf
With 182 enzymes: SgfI *
December 27, 2011 16:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1004 1788
Size: 1004 784
AatII G_ACGT'C
Cuts at: 0 1490 1788
Size: 1490 298
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 700 997 1201 1213 1276 1788
Size: 505 195 297 204 12 63 512
Fragments arranged by size:
512 505 297 204 195 63 12
AgeI A'CCGG_T
Cuts at: 0 104 1788
Size: 104 1684
AhdI GACnn_n'nnGTC
Cuts at: 0 1560 1788
Size: 1560 228
AleI CACnn'nnGTG
Cuts at: 0 595 1788
Size: 595 1193
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 520 552 1788
Size: 520 32 1236
Fragments arranged by size:
1236 520 32
AlwNI CAG_nnn'CTG
Cuts at: 0 483 795 807 837 990 1005 1071 1191
Size: 483 312 12 30 153 15 66 120
Cuts at: 1191 1305 1422 1431 1677 1698 1788
Size: 114 117 9 246 21 90
Fragments arranged by size:
483 312 246 153 120 117 114 90
66 30 21 15 12 9
ApaI G_GGCC'C
Cuts at: 0 745 925 1440 1788
Size: 745 180 515 348
Fragments arranged by size:
745 515 348 180
AvaI C'yCGr_G
Cuts at: 0 221 1045 1480 1566 1788
Size: 221 824 435 86 222
Fragments arranged by size:
824 435 222 221 86
BanI G'GyrC_C
Cuts at: 0 823 1312 1788
Size: 823 489 476
Fragments arranged by size:
823 489 476
BanII G_rGCy'C
Cuts at: 0 229 349 625 745 925 932 1046 1440
Size: 229 120 276 120 180 7 114 394
Cuts at: 1440 1727 1788
Size: 287 61
Fragments arranged by size:
394 287 276 229 180 120 120 114
61 7
BbeI G_GCGC'C
Cuts at: 0 827 1316 1788
Size: 827 489 472
Fragments arranged by size:
827 489 472
BbsI GAAGACnn'nnnn_
Cuts at: 0 194 1114 1788
Size: 194 920 674
Fragments arranged by size:
920 674 194
BbvCI CC'TCA_GC
Cuts at: 0 124 1291 1788
Size: 124 1167 497
Fragments arranged by size:
1167 497 124
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 340 374 1788
Size: 340 34 1414
Fragments arranged by size:
1414 340 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1450 1788
Size: 1450 338
BglII A'GATC_T
Cuts at: 0 1169 1788
Size: 1169 619
Bme1580I G_kGCm'C
Cuts at: 0 745 925 1440 1788
Size: 745 180 515 348
Fragments arranged by size:
745 515 348 180
BmrI ACTGGGnnnn_n'
Cuts at: 0 1134 1788
Size: 1134 654
BmtI G_CTAG'C
Cuts at: 0 1229 1788
Size: 1229 559
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 885 1788
Size: 885 903
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 640 814 817 855 937 1096 1150
Size: 443 197 174 3 38 82 159 54
Cuts at: 1150 1297 1306 1327 1354 1360 1432 1531 1699
Size: 147 9 21 27 6 72 99 168
Cuts at: 1699 1788
Size: 89
Fragments arranged by size:
443 197 174 168 159 147 99 89
82 72 54 38 27 21 9 6
3
Bpu10I CC'TnA_GC
Cuts at: 0 124 1087 1291 1788
Size: 124 963 204 497
Fragments arranged by size:
963 497 204 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 934 1228 1788
Size: 76 858 294 560
Fragments arranged by size:
858 560 294 76
BsaI GGTCTCn'nnnn_
Cuts at: 0 1072 1129 1246 1788
Size: 1072 57 117 542
Fragments arranged by size:
1072 542 117 57
BsaAI yAC'GTr
Cuts at: 0 1650 1705 1788
Size: 1650 55 83
Fragments arranged by size:
1650 83 55
BsaHI Gr'CG_yC
Cuts at: 0 824 1313 1487 1788
Size: 824 489 174 301
Fragments arranged by size:
824 489 301 174
BsaWI w'CCGG_w
Cuts at: 0 104 148 1788
Size: 104 44 1640
Fragments arranged by size:
1640 104 44
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1126 1156 1788
Size: 1126 30 632
Fragments arranged by size:
1126 632 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 408 868 901 1147 1552 1615 1788
Size: 408 460 33 246 405 63 173
Fragments arranged by size:
460 408 405 246 173 63 33
BseYI C'CCAG_C
Cuts at: 0 280 714 1788
Size: 280 434 1074
Fragments arranged by size:
1074 434 280
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 397 430 801 976 1788
Size: 380 17 33 371 175 812
Fragments arranged by size:
812 380 371 175 33 17
BsiEI CG_ry'CG
Cuts at: 0 525 895 1788
Size: 525 370 893
Fragments arranged by size:
893 525 370
BsiHKAI G_wGCw'C
Cuts at: 0 502 1046 1788
Size: 502 544 742
Fragments arranged by size:
742 544 502
Bsp1286I G_dGCh'C
Cuts at: 0 229 349 502 625 745 925 932 1046
Size: 229 120 153 123 120 180 7 114
Cuts at: 1046 1440 1727 1788
Size: 394 287 61
Fragments arranged by size:
394 287 229 180 153 123 120 120
114 61 7
BspHI T'CATG_A
Cuts at: 0 1055 1788
Size: 1055 733
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 975 990 1004 1079 1415 1788
Size: 975 15 14 75 336 373
Fragments arranged by size:
975 373 336 75 15 14
BsrBI CCG'CTC
Cuts at: 0 758 891 1515 1542 1584 1788
Size: 758 133 624 27 42 204
Fragments arranged by size:
758 624 204 133 42 27
BsrFI r'CCGG_y
Cuts at: 0 104 1788
Size: 104 1684
BssHII G'CGCG_C
Cuts at: 0 975 977 1788
Size: 975 2 811
Fragments arranged by size:
975 811 2
BssSI C'ACGA_G
Cuts at: 0 747 1588 1788
Size: 747 841 200
Fragments arranged by size:
841 747 200
BstAPI GCAn_nnn'nTGC
Cuts at: 0 50 990 1005 1071 1422 1431 1788
Size: 50 940 15 66 351 9 357
Fragments arranged by size:
940 357 351 66 50 15 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1332 1788
Size: 1332 456
BstYI r'GATC_y
Cuts at: 0 1169 1232 1788
Size: 1169 63 556
Fragments arranged by size:
1169 556 63
BtgI C'CryG_G
Cuts at: 0 394 596 770 895 1788
Size: 394 202 174 125 893
Fragments arranged by size:
893 394 202 174 125
BtsI GCAGTG_nn'
Cuts at: 0 48 368 1788
Size: 48 320 1420
Fragments arranged by size:
1420 320 48
DrdI GACnn_nn'nnGTC
Cuts at: 0 586 1788
Size: 586 1202
EaeI y'GGCC_r
Cuts at: 0 27 522 892 1652 1788
Size: 27 495 370 760 136
Fragments arranged by size:
760 495 370 136 27
EagI C'GGCC_G
Cuts at: 0 522 892 1788
Size: 522 370 896
Fragments arranged by size:
896 522 370
EarI CTCTTCn'nnn_
Cuts at: 0 961 1788
Size: 961 827
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 615 1396 1788
Size: 615 781 392
Fragments arranged by size:
781 615 392
EcoICRI GAG'CTC
Cuts at: 0 1044 1788
Size: 1044 744
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 505 640 700 814 817 855 937
Size: 443 62 135 60 114 3 38 82
Cuts at: 937 997 1096 1150 1201 1213 1276 1297 1306
Size: 60 99 54 51 12 63 21 9
Cuts at: 1306 1327 1354 1360 1432 1531 1699 1788
Size: 21 27 6 72 99 168 89
Fragments arranged by size:
443 168 135 114 99 99 89 82
72 63 62 60 60 54 51 38
27 21 21 12 9 6 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1057 1098 1681 1788
Size: 1057 41 583 107
Fragments arranged by size:
1057 583 107 41
EcoO109I rG'GnC_Cy
Cuts at: 0 601 741 742 921 987 1068 1091 1281
Size: 601 140 1 179 66 81 23 190
Cuts at: 1281 1295 1302 1329 1686 1788
Size: 14 7 27 357 102
Fragments arranged by size:
601 357 190 179 140 102 81 66
27 23 14 7 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1131 1788
Size: 1131 657
FspI TGC'GCA
Cuts at: 0 76 1321 1788
Size: 76 1245 467
Fragments arranged by size:
1245 467 76
HaeII r_GCGC'y
Cuts at: 0 511 827 903 1316 1677 1718 1788
Size: 511 316 76 413 361 41 70
Fragments arranged by size:
511 413 361 316 76 70 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1224 1256 1546 1578 1788
Size: 1224 32 290 32 210
Fragments arranged by size:
1224 290 210 32 32
HincII GTy'rAC
Cuts at: 0 1737 1788
Size: 1737 51
KasI G'GCGC_C
Cuts at: 0 823 1312 1788
Size: 823 489 476
Fragments arranged by size:
823 489 476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 722 791 1788
Size: 137 585 69 997
Fragments arranged by size:
997 585 137 69
MscI TGG'CCA
Cuts at: 0 1654 1788
Size: 1654 134
MslI CAynn'nnrTG
Cuts at: 0 595 1788
Size: 595 1193
MspA1I CmG'CkG
Cuts at: 0 260 444 576 585 897 1191 1254 1503
Size: 260 184 132 9 312 294 63 249
Cuts at: 1503 1635 1665 1698 1788
Size: 132 30 33 90
Fragments arranged by size:
312 294 260 249 184 132 132 90
63 33 30 9
NarI GG'CG_CC
Cuts at: 0 824 1313 1788
Size: 824 489 475
Fragments arranged by size:
824 489 475
NheI G'CTAG_C
Cuts at: 0 1225 1788
Size: 1225 563
NotI GC'GGCC_GC
Cuts at: 0 892 1788
Size: 892 896
PasI CC'CwG_GG
Cuts at: 0 642 1788
Size: 642 1146
PflMI CCAn_nnn'nTGG
Cuts at: 0 795 1305 1677 1788
Size: 795 510 372 111
Fragments arranged by size:
795 510 372 111
PfoI T'CCnGG_A
Cuts at: 0 656 788 1788
Size: 656 132 1000
Fragments arranged by size:
1000 656 132
PmlI CAC'GTG
Cuts at: 0 1705 1788
Size: 1705 83
PpuMI rG'GwC_Cy
Cuts at: 0 601 987 1068 1091 1295 1302 1788
Size: 601 386 81 23 204 7 486
Fragments arranged by size:
601 486 386 204 81 23 7
PshAI GACnn'nnGTC
Cuts at: 0 149 1788
Size: 149 1639
PspOMI G'GGCC_C
Cuts at: 0 741 921 1436 1788
Size: 741 180 515 352
Fragments arranged by size:
741 515 352 180
PstI C_TGCA'G
Cuts at: 0 842 907 1001 1010 1364 1427 1502 1640
Size: 842 65 94 9 354 63 75 138
Cuts at: 1640 1694 1788
Size: 54 94
Fragments arranged by size:
842 354 138 94 94 75 65 63
54 9
PvuII CAG'CTG
Cuts at: 0 444 576 1191 1254 1635 1698 1788
Size: 444 132 615 63 381 63 90
Fragments arranged by size:
615 444 381 132 90 63 63
SacI G_AGCT'C
Cuts at: 0 1046 1788
Size: 1046 742
SacII CC_GC'GG
Cuts at: 0 898 1788
Size: 898 890
SanDI GG'GwC_CC
Cuts at: 0 601 1788
Size: 601 1187
SapI GCTCTTCn'nnn_
Cuts at: 0 961 1788
Size: 961 827
SbfI CC_TGCA'GG
Cuts at: 0 1001 1788
Size: 1001 787
SfcI C'TryA_G
Cuts at: 0 838 903 997 1006 1360 1423 1498 1636
Size: 838 65 94 9 354 63 75 138
Cuts at: 1636 1690 1788
Size: 54 98
Fragments arranged by size:
838 354 138 98 94 75 65 63
54 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1450 1788
Size: 1450 338
SfoI GGC'GCC
Cuts at: 0 825 1314 1788
Size: 825 489 474
Fragments arranged by size:
825 489 474
SmaI CCC'GGG
Cuts at: 0 223 1788
Size: 223 1565
SmlI C'TyrA_G
Cuts at: 0 91 949 1045 1243 1788
Size: 91 858 96 198 545
Fragments arranged by size:
858 545 198 96 91
StuI AGG'CCT
Cuts at: 0 958 1788
Size: 958 830
StyI C'CwwG_G
Cuts at: 0 470 1220 1325 1727 1788
Size: 470 750 105 402 61
Fragments arranged by size:
750 470 402 105 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 251 655 1788
Size: 251 404 1133
Fragments arranged by size:
1133 404 251
TatI w'GTAC_w
Cuts at: 0 65 1788
Size: 65 1723
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 145 177 1126 1158 1788
Size: 145 32 949 32 630
Fragments arranged by size:
949 630 145 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 171 1788
Size: 171 1617
XhoI C'TCGA_G
Cuts at: 0 1045 1788
Size: 1045 743
XmaI C'CCGG_G
Cuts at: 0 221 1788
Size: 221 1567
ZraI GAC'GTC
Cuts at: 0 1488 1788
Size: 1488 300
Enzymes that do cut:
AarI AatII AcuI AgeI AhdI AleI AloI AlwNI
ApaI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BglI BglII Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstXI BstYI BtgI BtsI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NarI NheI NotI PasI PflMI PfoI
PmlI PpuMI PshAI PspOMI PstI PvuII SacI SacII
SanDI SapI SbfI SfcI SfiI SfoI SmaI SmlI
StuI StyI TaqII TatI TstI XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AlfI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI
BclI BfrBI BlpI BmgBI BsaBI BsiWI BsmI BsmBI
BspEI BsrDI BsrGI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII EcoRI EcoRV FseI FspAI
HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI
NgoMIV NruI NsiI NspI PacI PciI PmeI PpiI
PsiI PspXI PsrI PvuI RsrII SalI ScaI SexAI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XmnI