Restriction map for
pFN21AB6104
(Linear) (Six-base) MAPSORT of: KIBB6104.seq Check: 9699 from: 1 to: 1842
KIBB6104.orf
With 182 enzymes: SgfI *
January 7, 2013 14:38 ..
AatII G_ACGT'C
Cuts at: 0 662 1842
Size: 662 1180
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 1493 1842
Size: 586 907 349
Fragments arranged by size:
907 586 349
AflIII A'CryG_T
Cuts at: 0 198 1409 1842
Size: 198 1211 433
Fragments arranged by size:
1211 433 198
AhdI GACnn_n'nnGTC
Cuts at: 0 1565 1842
Size: 1565 277
AleI CACnn'nnGTG
Cuts at: 0 308 1842
Size: 308 1534
AlwNI CAG_nnn'CTG
Cuts at: 0 123 1842
Size: 123 1719
ApoI r'AATT_y
Cuts at: 0 12 756 832 1842
Size: 12 744 76 1010
Fragments arranged by size:
1010 744 76 12
AvaI C'yCGr_G
Cuts at: 0 1158 1184 1361 1500 1842
Size: 1158 26 177 139 342
Fragments arranged by size:
1158 342 177 139 26
BanI G'GyrC_C
Cuts at: 0 377 890 1842
Size: 377 513 952
Fragments arranged by size:
952 513 377
BanII G_rGCy'C
Cuts at: 0 1507 1842
Size: 1507 335
BbsI GAAGACnn'nnnn_
Cuts at: 0 1208 1484 1842
Size: 1208 276 358
Fragments arranged by size:
1208 358 276
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 255 289 1842
Size: 255 34 1553
Fragments arranged by size:
1553 255 34
BclI T'GATC_A
Cuts at: 0 296 341 1842
Size: 296 45 1501
Fragments arranged by size:
1501 296 45
BglI GCCn_nnn'nGGC
Cuts at: 0 457 1842
Size: 457 1385
Bme1580I G_kGCm'C
Cuts at: 0 895 1842
Size: 895 947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 888 1006 1197 1390 1842
Size: 888 118 191 193 452
Fragments arranged by size:
888 452 193 191 118
Bpu10I CC'TnA_GC
Cuts at: 0 145 1842
Size: 145 1697
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1623 1842
Size: 1623 219
BsaHI Gr'CG_yC
Cuts at: 0 659 1842
Size: 659 1183
BsaWI w'CCGG_w
Cuts at: 0 977 1842
Size: 977 865
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1195 1225 1842
Size: 1195 30 617
Fragments arranged by size:
1195 617 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 436 1842
Size: 436 1406
BseYI C'CCAG_C
Cuts at: 0 1742 1842
Size: 1742 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 1842
Size: 334 1508
BsiEI CG_ry'CG
Cuts at: 0 1072 1842
Size: 1072 770
BsiHKAI G_wGCw'C
Cuts at: 0 416 1507 1842
Size: 416 1091 335
Fragments arranged by size:
1091 416 335
Bsp1286I G_dGCh'C
Cuts at: 0 416 895 1507 1842
Size: 416 479 612 335
Fragments arranged by size:
612 479 416 335
BspEI T'CCGG_A
Cuts at: 0 977 1842
Size: 977 865
BspHI T'CATG_A
Cuts at: 0 1815 1842
Size: 1815 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 149 1252 1642 1842
Size: 149 1103 390 200
Fragments arranged by size:
1103 390 200 149
BsrDI GCAATG_nn'
Cuts at: 0 1646 1842
Size: 1646 196
BsrFI r'CCGG_y
Cuts at: 0 1307 1842
Size: 1307 535
BstAPI GCAn_nnn'nTGC
Cuts at: 0 53 448 1733 1842
Size: 53 395 1285 109
Fragments arranged by size:
1285 395 109 53
BstXI CCAn_nnnn'nTGG
Cuts at: 0 169 248 1842
Size: 169 79 1594
Fragments arranged by size:
1594 169 79
BstYI r'GATC_y
Cuts at: 0 1365 1806 1842
Size: 1365 441 36
Fragments arranged by size:
1365 441 36
Bsu36I CC'TnA_GG
Cuts at: 0 1281 1842
Size: 1281 561
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 695 1842
Size: 695 1147
BtsI GCAGTG_nn'
Cuts at: 0 54 1095 1224 1667 1753 1842
Size: 54 1041 129 443 86 89
Fragments arranged by size:
1041 443 129 89 86 54
DraIII CAC_nnn'GTG
Cuts at: 0 890 1842
Size: 890 952
EaeI y'GGCC_r
Cuts at: 0 1533 1695 1842
Size: 1533 162 147
Fragments arranged by size:
1533 162 147
EarI CTCTTCn'nnn_
Cuts at: 0 761 992 1310 1379 1512 1556 1842
Size: 761 231 318 69 133 44 286
Fragments arranged by size:
761 318 286 231 133 69 44
EcoICRI GAG'CTC
Cuts at: 0 1505 1842
Size: 1505 337
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 888 1006 1197 1390 1493 1842
Size: 586 302 118 191 193 103 349
Fragments arranged by size:
586 349 302 193 191 118 103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 640 1842
Size: 640 1202
FspI TGC'GCA
Cuts at: 0 1645 1842
Size: 1645 197
HaeII r_GCGC'y
Cuts at: 0 1116 1842
Size: 1116 726
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 96 128 1195 1227 1551 1583 1842
Size: 96 32 1067 32 324 32 259
Fragments arranged by size:
1067 324 259 96 32 32 32
HindIII A'AGCT_T
Cuts at: 0 223 1525 1842
Size: 223 1302 317
Fragments arranged by size:
1302 317 223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 672 786 1549 1842
Size: 672 114 763 293
Fragments arranged by size:
763 672 293 114
MscI TGG'CCA
Cuts at: 0 1535 1697 1842
Size: 1535 162 145
Fragments arranged by size:
1535 162 145
MslI CAynn'nnrTG
Cuts at: 0 308 1438 1842
Size: 308 1130 404
Fragments arranged by size:
1130 404 308
MspA1I CmG'CkG
Cuts at: 0 1059 1082 1842
Size: 1059 23 760
Fragments arranged by size:
1059 760 23
NspI r_CATG'y
Cuts at: 0 202 585 1413 1437 1842
Size: 202 383 828 24 405
Fragments arranged by size:
828 405 383 202 24
PciI A'CATG_T
Cuts at: 0 198 1409 1842
Size: 198 1211 433
Fragments arranged by size:
1211 433 198
PflMI CCAn_nnn'nTGG
Cuts at: 0 123 1704 1842
Size: 123 1581 138
Fragments arranged by size:
1581 138 123
PspXI vC'TCGA_Gb
Cuts at: 0 1500 1842
Size: 1500 342
PstI C_TGCA'G
Cuts at: 0 458 959 1249 1842
Size: 458 501 290 593
Fragments arranged by size:
593 501 458 290
PvuII CAG'CTG
Cuts at: 0 1059 1842
Size: 1059 783
SacI G_AGCT'C
Cuts at: 0 1507 1842
Size: 1507 335
SapI GCTCTTCn'nnn_
Cuts at: 0 1512 1842
Size: 1512 330
SbfI CC_TGCA'GG
Cuts at: 0 458 1842
Size: 458 1384
ScaI AGT'ACT
Cuts at: 0 319 1542 1842
Size: 319 1223 300
Fragments arranged by size:
1223 319 300
SfcI C'TryA_G
Cuts at: 0 454 675 955 1245 1842
Size: 454 221 280 290 597
Fragments arranged by size:
597 454 290 280 221
SmaI CCC'GGG
Cuts at: 0 1186 1363 1842
Size: 1186 177 479
Fragments arranged by size:
1186 479 177
SmlI C'TyrA_G
Cuts at: 0 1500 1602 1842
Size: 1500 102 240
Fragments arranged by size:
1500 240 102
SspI AAT'ATT
Cuts at: 0 235 1842
Size: 235 1607
StuI AGG'CCT
Cuts at: 0 427 1718 1842
Size: 427 1291 124
Fragments arranged by size:
1291 427 124
TatI w'GTAC_w
Cuts at: 0 317 1540 1842
Size: 317 1223 302
Fragments arranged by size:
1223 317 302
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 800 832 1132 1164 1842
Size: 800 32 300 32 678
Fragments arranged by size:
800 678 300 32 32
XhoI C'TCGA_G
Cuts at: 0 1500 1842
Size: 1500 342
XmaI C'CCGG_G
Cuts at: 0 1184 1361 1842
Size: 1184 177 481
Fragments arranged by size:
1184 481 177
XmnI GAAnn'nnTTC
Cuts at: 0 1636 1842
Size: 1636 206
ZraI GAC'GTC
Cuts at: 0 660 1842
Size: 660 1182
Enzymes that do cut:
AatII AcuI AflIII AhdI AleI AlwNI ApoI AvaI
BanI BanII BbsI BcgI BclI BglI Bme1580I BpmI
Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI
BstAPI BstXI BstYI Bsu36I BtgZI BtsI DraIII EaeI
EarI EcoICRI Eco57MI FalI FspI HaeII Hin4I HindIII
MmeI MscI MslI MspA1I NspI PciI PflMI PspXI
PstI PvuII SacI SapI SbfI ScaI SfcI SmaI
SmlI SspI StuI TatI TstI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BciVI BfrBI BglII BlpI BmgBI
BmrI BmtI BplI BsaI BsaAI BsaBI BsiWI BsmI
BsmBI BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I
BtgI ClaI CspCI DraI DrdI EagI EciI EcoNI
EcoO109I EcoRI EcoRV FseI FspAI HincII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PvuI
RsrII SacII SalI SanDI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI StyI SwaI TaqII
Tth111I XbaI XcmI