Restriction map for
pFN21AB5970
(Linear) (Six-base) MAPSORT of: KIBB5970.seq Check: 3160 from: 1 to: 1680
KIBB5970.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AccI GT'mk_AC
Cuts at: 0 1406 1680
Size: 1406 274
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1303 1552 1680
Size: 1303 249 128
Fragments arranged by size:
1303 249 128
AfeI AGC'GCT
Cuts at: 0 1271 1423 1680
Size: 1271 152 257
Fragments arranged by size:
1271 257 152
AgeI A'CCGG_T
Cuts at: 0 313 1680
Size: 313 1367
AhdI GACnn_n'nnGTC
Cuts at: 0 1098 1680
Size: 1098 582
AlwNI CAG_nnn'CTG
Cuts at: 0 387 696 1299 1680
Size: 387 309 603 381
Fragments arranged by size:
603 387 381 309
ApaI G_GGCC'C
Cuts at: 0 270 280 1680
Size: 270 10 1400
Fragments arranged by size:
1400 270 10
AscI GG'CGCG_CC
Cuts at: 0 1126 1213 1561 1680
Size: 1126 87 348 119
Fragments arranged by size:
1126 348 119 87
AvaI C'yCGr_G
Cuts at: 0 281 569 838 917 1179 1438 1680
Size: 281 288 269 79 262 259 242
Fragments arranged by size:
288 281 269 262 259 242 79
BamHI G'GATC_C
Cuts at: 0 945 1074 1323 1680
Size: 945 129 249 357
Fragments arranged by size:
945 357 249 129
BanI G'GyrC_C
Cuts at: 0 97 547 1104 1680
Size: 97 450 557 576
Fragments arranged by size:
576 557 450 97
BanII G_rGCy'C
Cuts at: 0 54 225 270 280 452 770 1400 1680
Size: 54 171 45 10 172 318 630 280
Fragments arranged by size:
630 318 280 172 171 54 45 10
BbeI G_GCGC'C
Cuts at: 0 1108 1680
Size: 1108 572
BbsI GAAGACnn'nnnn_
Cuts at: 0 128 1680
Size: 128 1552
BbvCI CC'TCA_GC
Cuts at: 0 436 1048 1680
Size: 436 612 632
Fragments arranged by size:
632 612 436
BglI GCCn_nnn'nGGC
Cuts at: 0 49 822 1238 1247 1549 1558 1680
Size: 49 773 416 9 302 9 122
Fragments arranged by size:
773 416 302 122 49 9 9
BlpI GC'TnA_GC
Cuts at: 0 1399 1680
Size: 1399 281
Bme1580I G_kGCm'C
Cuts at: 0 100 270 280 552 809 1347 1583 1649
Size: 100 170 10 272 257 538 236 66
Cuts at: 1649 1680
Size: 31
Fragments arranged by size:
538 272 257 236 170 100 66 31
10
BmgBI CAC'GTC
Cuts at: 0 1100 1680
Size: 1100 580
BmrI ACTGGGnnnn_n'
Cuts at: 0 184 1680
Size: 184 1496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 424 479 892 1027 1680
Size: 122 302 55 413 135 653
Fragments arranged by size:
653 413 302 135 122 55
Bpu10I CC'TnA_GC
Cuts at: 0 436 1048 1680
Size: 436 612 632
Fragments arranged by size:
632 612 436
BsaI GGTCTCn'nnnn_
Cuts at: 0 278 1435 1680
Size: 278 1157 245
Fragments arranged by size:
1157 278 245
BsaAI yAC'GTr
Cuts at: 0 1518 1644 1680
Size: 1518 126 36
Fragments arranged by size:
1518 126 36
BsaHI Gr'CG_yC
Cuts at: 0 1105 1680
Size: 1105 575
BsaWI w'CCGG_w
Cuts at: 0 313 948 1680
Size: 313 635 732
Fragments arranged by size:
732 635 313
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 476 856 1042 1636 1680
Size: 97 379 380 186 594 44
Fragments arranged by size:
594 380 379 186 97 44
BseYI C'CCAG_C
Cuts at: 0 57 272 351 535 853 1680
Size: 57 215 79 184 318 827
Fragments arranged by size:
827 318 215 184 79 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 1072 1680
Size: 280 792 608
Fragments arranged by size:
792 608 280
BsiEI CG_ry'CG
Cuts at: 0 1494 1680
Size: 1494 186
BsiHKAI G_wGCw'C
Cuts at: 0 1400 1680
Size: 1400 280
BsmBI CGTCTCn'nnnn_
Cuts at: 0 868 1680
Size: 868 812
Bsp1286I G_dGCh'C
Cuts at: 0 54 100 225 270 280 452 552 770
Size: 54 46 125 45 10 172 100 218
Cuts at: 770 809 1347 1400 1583 1649 1680
Size: 39 538 53 183 66 31
Fragments arranged by size:
538 218 183 172 125 100 66 54
53 46 45 39 31 10
BspEI T'CCGG_A
Cuts at: 0 948 1680
Size: 948 732
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 17 1680
Size: 17 1663
BsrDI GCAATG_nn'
Cuts at: 0 195 1680
Size: 195 1485
BsrFI r'CCGG_y
Cuts at: 0 313 961 1680
Size: 313 648 719
Fragments arranged by size:
719 648 313
BssHII G'CGCG_C
Cuts at: 0 1126 1213 1385 1561 1634 1680
Size: 1126 87 172 176 73 46
Fragments arranged by size:
1126 176 172 87 73 46
BstAPI GCAn_nnn'nTGC
Cuts at: 0 696 1299 1644 1680
Size: 696 603 345 36
Fragments arranged by size:
696 603 345 36
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1122 1656 1680
Size: 1122 534 24
Fragments arranged by size:
1122 534 24
BstYI r'GATC_y
Cuts at: 0 945 1074 1323 1680
Size: 945 129 249 357
Fragments arranged by size:
945 357 249 129
Bsu36I CC'TnA_GG
Cuts at: 0 515 1680
Size: 515 1165
BtgI C'CryG_G
Cuts at: 0 654 757 830 998 1108 1115 1545 1680
Size: 654 103 73 168 110 7 430 135
Fragments arranged by size:
654 430 168 135 110 103 73 7
BtsI GCAGTG_nn'
Cuts at: 0 694 1055 1680
Size: 694 361 625
Fragments arranged by size:
694 625 361
DraIII CAC_nnn'GTG
Cuts at: 0 1521 1644 1667 1680
Size: 1521 123 23 13
Fragments arranged by size:
1521 123 23 13
EaeI y'GGCC_r
Cuts at: 0 290 789 959 1061 1193 1658 1680
Size: 290 499 170 102 132 465 22
Fragments arranged by size:
499 465 290 170 132 102 22
EcoICRI GAG'CTC
Cuts at: 0 1398 1680
Size: 1398 282
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 424 479 892 1027 1303 1552 1680
Size: 122 302 55 413 135 276 249 128
Fragments arranged by size:
413 302 276 249 135 128 122 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 435 1618 1680
Size: 435 1183 62
Fragments arranged by size:
1183 435 62
EcoO109I rG'GnC_Cy
Cuts at: 0 6 69 266 276 277 356 369 614
Size: 6 63 197 10 1 79 13 245
Cuts at: 614 906 975 1240 1296 1680
Size: 292 69 265 56 384
Fragments arranged by size:
384 292 265 245 197 79 69 63
56 13 10 6 1
FspI TGC'GCA
Cuts at: 0 785 1680
Size: 785 895
HaeII r_GCGC'y
Cuts at: 0 233 387 487 901 1006 1014 1108 1273
Size: 233 154 100 414 105 8 94 165
Cuts at: 1273 1291 1425 1680
Size: 18 134 255
Fragments arranged by size:
414 255 233 165 154 134 105 100
94 18 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1084 1116 1680
Size: 1084 32 564
Fragments arranged by size:
1084 564 32
HincII GTy'rAC
Cuts at: 0 984 1680
Size: 984 696
KasI G'GCGC_C
Cuts at: 0 1104 1680
Size: 1104 576
MfeI C'AATT_G
Cuts at: 0 1458 1680
Size: 1458 222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 971 1514 1598 1680
Size: 971 543 84 82
Fragments arranged by size:
971 543 84 82
MscI TGG'CCA
Cuts at: 0 292 1660 1680
Size: 292 1368 20
Fragments arranged by size:
1368 292 20
MslI CAynn'nnrTG
Cuts at: 0 730 1680
Size: 730 950
MspA1I CmG'CkG
Cuts at: 0 91 272 656 719 759 777 795 832
Size: 91 181 384 63 40 18 18 37
Cuts at: 832 1000 1035 1206 1680
Size: 168 35 171 474
Fragments arranged by size:
474 384 181 171 168 91 63 40
37 35 18 18
NaeI GCC'GGC
Cuts at: 0 963 1680
Size: 963 717
NarI GG'CG_CC
Cuts at: 0 1105 1680
Size: 1105 575
NgoMIV G'CCGG_C
Cuts at: 0 961 1680
Size: 961 719
PasI CC'CwG_GG
Cuts at: 0 45 373 502 1680
Size: 45 328 129 1178
Fragments arranged by size:
1178 328 129 45
PfoI T'CCnGG_A
Cuts at: 0 443 869 1680
Size: 443 426 811
Fragments arranged by size:
811 443 426
PmlI CAC'GTG
Cuts at: 0 1518 1644 1680
Size: 1518 126 36
Fragments arranged by size:
1518 126 36
PpuMI rG'GwC_Cy
Cuts at: 0 6 369 906 1680
Size: 6 363 537 774
Fragments arranged by size:
774 537 363 6
PspOMI G'GGCC_C
Cuts at: 0 266 276 1680
Size: 266 10 1404
Fragments arranged by size:
1404 266 10
PspXI vC'TCGA_Gb
Cuts at: 0 838 1680
Size: 838 842
PstI C_TGCA'G
Cuts at: 0 650 692 1295 1680
Size: 650 42 603 385
Fragments arranged by size:
650 603 385 42
PvuII CAG'CTG
Cuts at: 0 91 795 1680
Size: 91 704 885
Fragments arranged by size:
885 704 91
RsrII CG'GwC_CG
Cuts at: 0 761 1680
Size: 761 919
SacI G_AGCT'C
Cuts at: 0 1400 1680
Size: 1400 280
SacII CC_GC'GG
Cuts at: 0 657 760 833 1001 1680
Size: 657 103 73 168 679
Fragments arranged by size:
679 657 168 103 73
SanDI GG'GwC_CC
Cuts at: 0 369 906 1680
Size: 369 537 774
Fragments arranged by size:
774 537 369
SexAI A'CCwGG_T
Cuts at: 0 584 1680
Size: 584 1096
SfcI C'TryA_G
Cuts at: 0 646 688 1291 1680
Size: 646 42 603 389
Fragments arranged by size:
646 603 389 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1247 1549 1680
Size: 1247 302 131
Fragments arranged by size:
1247 302 131
SfoI GGC'GCC
Cuts at: 0 1106 1680
Size: 1106 574
SgrAI Cr'CCGG_yG
Cuts at: 0 313 1680
Size: 313 1367
SmaI CCC'GGG
Cuts at: 0 919 1181 1680
Size: 919 262 499
Fragments arranged by size:
919 499 262
SmlI C'TyrA_G
Cuts at: 0 838 1680
Size: 838 842
SrfI GCCC'GGGC
Cuts at: 0 919 1680
Size: 919 761
StuI AGG'CCT
Cuts at: 0 244 1680
Size: 244 1436
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 867 1680
Size: 867 813
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1059 1091 1680
Size: 1059 32 589
Fragments arranged by size:
1059 589 32
XhoI C'TCGA_G
Cuts at: 0 838 1680
Size: 838 842
XmaI C'CCGG_G
Cuts at: 0 917 1179 1680
Size: 917 262 501
Fragments arranged by size:
917 501 262
Enzymes that do cut:
AccI AcuI AfeI AgeI AhdI AlwNI ApaI AscI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrDI BsrFI BssHII BstAPI BstXI
BstYI Bsu36I BtgI BtsI DraIII EaeI EcoICRI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I HincII KasI MfeI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV PasI
PfoI PmlI PpuMI PspOMI PspXI PstI PvuII RsrII
SacI SacII SanDI SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI SrfI StuI TaqII TstI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AleI AlfI
AloI ApaLI ApoI AseI AsiSI AvrII BaeI BcgI
BciVI BclI BfrBI BglII BmtI BplI BpuEI BsaBI
BsaXI BsiWI BsmI BspHI BsrBI BsrGI BssSI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EagI
EarI EciI EcoRI EcoRV FalI FseI FspAI HindIII
HpaI KpnI MluI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PpiI PshAI
PsiI PsrI PvuI SalI SapI SbfI ScaI SgfI
SnaBI SpeI SphI SspI StyI SwaI TatI Tth111I
XbaI XcmI XmnI ZraI