Restriction map for
pFN21AB5934
(Linear) (Six-base) MAPSORT of: KIBB5934.seq Check: 187 from: 1 to: 1614
KIBB5934.orf
With 182 enzymes: SgfI *
July 3, 2012 16:12 ..
AccI GT'mk_AC
Cuts at: 0 43 1614
Size: 43 1571
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1119 1203 1614
Size: 1119 84 411
Fragments arranged by size:
1119 411 84
AfeI AGC'GCT
Cuts at: 0 142 1614
Size: 142 1472
AflIII A'CryG_T
Cuts at: 0 868 1614
Size: 868 746
AleI CACnn'nnGTG
Cuts at: 0 1010 1094 1508 1614
Size: 1010 84 414 106
Fragments arranged by size:
1010 414 106 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 802 935 1614
Size: 802 133 679
Fragments arranged by size:
802 679 133
AlwNI CAG_nnn'CTG
Cuts at: 0 222 255 282 880 1614
Size: 222 33 27 598 734
Fragments arranged by size:
734 598 222 33 27
ApoI r'AATT_y
Cuts at: 0 1556 1614
Size: 1556 58
BamHI G'GATC_C
Cuts at: 0 526 1337 1614
Size: 526 811 277
Fragments arranged by size:
811 526 277
BanI G'GyrC_C
Cuts at: 0 393 1614
Size: 393 1221
BanII G_rGCy'C
Cuts at: 0 133 209 1063 1614
Size: 133 76 854 551
Fragments arranged by size:
854 551 133 76
BbeI G_GCGC'C
Cuts at: 0 397 1614
Size: 397 1217
BbsI GAAGACnn'nnnn_
Cuts at: 0 56 854 1219 1614
Size: 56 798 365 395
Fragments arranged by size:
798 395 365 56
BbvCI CC'TCA_GC
Cuts at: 0 797 1614
Size: 797 817
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 191 225 1614
Size: 191 34 1389
Fragments arranged by size:
1389 191 34
BglII A'GATC_T
Cuts at: 0 114 1614
Size: 114 1500
BmrI ACTGGGnnnn_n'
Cuts at: 0 33 98 1187 1271 1355 1614
Size: 33 65 1089 84 84 259
Fragments arranged by size:
1089 259 84 84 65 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 986 1614
Size: 986 628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 292 538 605 776 1614
Size: 277 15 246 67 171 838
Fragments arranged by size:
838 277 246 171 67 15
Bpu10I CC'TnA_GC
Cuts at: 0 797 978 1370 1614
Size: 797 181 392 244
Fragments arranged by size:
797 392 244 181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1614
Size: 493 1121
BsaI GGTCTCn'nnnn_
Cuts at: 0 753 1614
Size: 753 861
BsaHI Gr'CG_yC
Cuts at: 0 394 1614
Size: 394 1220
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1258 1288 1614
Size: 1258 30 326
Fragments arranged by size:
1258 326 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 358 814 1614
Size: 358 456 800
Fragments arranged by size:
800 456 358
BseYI C'CCAG_C
Cuts at: 0 311 530 1614
Size: 311 219 1084
Fragments arranged by size:
1084 311 219
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 893 1302 1614
Size: 893 409 312
Fragments arranged by size:
893 409 312
BsiHKAI G_wGCw'C
Cuts at: 0 209 1063 1614
Size: 209 854 551
Fragments arranged by size:
854 551 209
Bsp1286I G_dGCh'C
Cuts at: 0 133 209 1063 1614
Size: 133 76 854 551
Fragments arranged by size:
854 551 133 76
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 390 1614
Size: 390 1224
BsrBI CCG'CTC
Cuts at: 0 338 1614
Size: 338 1276
BsrDI GCAATG_nn'
Cuts at: 0 851 1614
Size: 851 763
BsrFI r'CCGG_y
Cuts at: 0 94 390 1614
Size: 94 296 1224
Fragments arranged by size:
1224 296 94
BstAPI GCAn_nnn'nTGC
Cuts at: 0 429 1614
Size: 429 1185
BstXI CCAn_nnnn'nTGG
Cuts at: 0 955 1614
Size: 955 659
BstYI r'GATC_y
Cuts at: 0 114 251 526 1337 1614
Size: 114 137 275 811 277
Fragments arranged by size:
811 277 275 137 114
BtgI C'CryG_G
Cuts at: 0 573 735 1614
Size: 573 162 879
Fragments arranged by size:
879 573 162
BtsI GCAGTG_nn'
Cuts at: 0 605 1614
Size: 605 1009
EarI CTCTTCn'nnn_
Cuts at: 0 1028 1614
Size: 1028 586
EcoICRI GAG'CTC
Cuts at: 0 207 1061 1614
Size: 207 854 553
Fragments arranged by size:
854 553 207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 292 538 605 776 1119 1203 1614
Size: 277 15 246 67 171 343 84 411
Fragments arranged by size:
411 343 277 246 171 84 67 15
EcoO109I rG'GnC_Cy
Cuts at: 0 175 558 1614
Size: 175 383 1056
Fragments arranged by size:
1056 383 175
EcoRV GAT'ATC
Cuts at: 0 830 1614
Size: 830 784
HaeII r_GCGC'y
Cuts at: 0 144 190 397 1614
Size: 144 46 207 1217
Fragments arranged by size:
1217 207 144 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 25 57 1614
Size: 25 32 1557
Fragments arranged by size:
1557 32 25
HincII GTy'rAC
Cuts at: 0 414 1614
Size: 414 1200
KasI G'GCGC_C
Cuts at: 0 393 1614
Size: 393 1221
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1391 1614
Size: 1391 223
MslI CAynn'nnrTG
Cuts at: 0 76 953 1010 1094 1110 1508 1614
Size: 76 877 57 84 16 398 106
Fragments arranged by size:
877 398 106 84 76 57 16
MspA1I CmG'CkG
Cuts at: 0 381 796 1614
Size: 381 415 818
Fragments arranged by size:
818 415 381
NaeI GCC'GGC
Cuts at: 0 392 1614
Size: 392 1222
NarI GG'CG_CC
Cuts at: 0 394 1614
Size: 394 1220
NcoI C'CATG_G
Cuts at: 0 573 735 1614
Size: 573 162 879
Fragments arranged by size:
879 573 162
NdeI CA'TA_TG
Cuts at: 0 1107 1614
Size: 1107 507
NgoMIV G'CCGG_C
Cuts at: 0 390 1614
Size: 390 1224
NspI r_CATG'y
Cuts at: 0 872 1614
Size: 872 742
PasI CC'CwG_GG
Cuts at: 0 466 630 753 1614
Size: 466 164 123 861
Fragments arranged by size:
861 466 164 123
PciI A'CATG_T
Cuts at: 0 868 1614
Size: 868 746
PfoI T'CCnGG_A
Cuts at: 0 254 1614
Size: 254 1360
PpuMI rG'GwC_Cy
Cuts at: 0 558 1614
Size: 558 1056
PsiI TTA'TAA
Cuts at: 0 1439 1614
Size: 1439 175
PstI C_TGCA'G
Cuts at: 0 174 227 308 589 1234 1614
Size: 174 53 81 281 645 380
Fragments arranged by size:
645 380 281 174 81 53
PvuII CAG'CTG
Cuts at: 0 381 796 1614
Size: 381 415 818
Fragments arranged by size:
818 415 381
SacI G_AGCT'C
Cuts at: 0 209 1063 1614
Size: 209 854 551
Fragments arranged by size:
854 551 209
SfcI C'TryA_G
Cuts at: 0 44 170 223 304 585 711 1230 1614
Size: 44 126 53 81 281 126 519 384
Fragments arranged by size:
519 384 281 126 126 81 53 44
SfoI GGC'GCC
Cuts at: 0 395 1614
Size: 395 1219
SmlI C'TyrA_G
Cuts at: 0 508 1614
Size: 508 1106
StuI AGG'CCT
Cuts at: 0 983 1132 1384 1481 1614
Size: 983 149 252 97 133
Fragments arranged by size:
983 252 149 133 97
StyI C'CwwG_G
Cuts at: 0 573 735 1614
Size: 573 162 879
Fragments arranged by size:
879 573 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 303 745 1614
Size: 303 442 869
Fragments arranged by size:
869 442 303
XmnI GAAnn'nnTTC
Cuts at: 0 1216 1614
Size: 1216 398
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AlfI AlwNI ApoI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BglII
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI
BsrFI BstAPI BstXI BstYI BtgI BtsI EarI EcoICRI
Eco57MI EcoO109I EcoRV HaeII Hin4I HincII KasI MmeI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PfoI PpuMI PsiI PstI PvuII SacI
SfcI SfoI SmlI StuI StyI TaqII XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI
BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoNI EcoRI FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MscI NheI NotI NruI
NsiI PacI PflMI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI