Restriction map for
pFN21AB5932
(Linear) (Six-base) MAPSORT of: KIBB5932.seq Check: 8792 from: 1 to: 1614
KIBB5932.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 291 1614
Size: 291 1323
Acc65I G'GTAC_C
Cuts at: 0 1207 1614
Size: 1207 407
AclI AA'CG_TT
Cuts at: 0 887 1614
Size: 887 727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 1221 1451 1614
Size: 367 854 230 163
Fragments arranged by size:
854 367 230 163
AhdI GACnn_n'nnGTC
Cuts at: 0 687 1614
Size: 687 927
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 574 1614
Size: 574 1040
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1081 1113 1614
Size: 1081 32 501
Fragments arranged by size:
1081 501 32
AlwNI CAG_nnn'CTG
Cuts at: 0 357 1614
Size: 357 1257
AvaI C'yCGr_G
Cuts at: 0 228 295 307 905 1614
Size: 228 67 12 598 709
Fragments arranged by size:
709 598 228 67 12
BamHI G'GATC_C
Cuts at: 0 610 1614
Size: 610 1004
BanI G'GyrC_C
Cuts at: 0 211 224 303 996 1207 1414 1614
Size: 211 13 79 693 211 207 200
Fragments arranged by size:
693 211 211 207 200 79 13
BbeI G_GCGC'C
Cuts at: 0 215 1614
Size: 215 1399
BbsI GAAGACnn'nnnn_
Cuts at: 0 1457 1614
Size: 1457 157
BbvCI CC'TCA_GC
Cuts at: 0 917 1614
Size: 917 697
BciVI GTATCCnnnnn_n'
Cuts at: 0 630 1614
Size: 630 984
BglII A'GATC_T
Cuts at: 0 422 1614
Size: 422 1192
BlpI GC'TnA_GC
Cuts at: 0 815 1614
Size: 815 799
Bme1580I G_kGCm'C
Cuts at: 0 227 308 1614
Size: 227 81 1306
Fragments arranged by size:
1306 227 81
BmrI ACTGGGnnnn_n'
Cuts at: 0 610 1099 1609 1614
Size: 610 489 510 5
Fragments arranged by size:
610 510 489 5
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 333 1614
Size: 333 1281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 791 1614
Size: 791 823
Bpu10I CC'TnA_GC
Cuts at: 0 917 1614
Size: 917 697
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 1614
Size: 141 1473
BsaI GGTCTCn'nnnn_
Cuts at: 0 710 1240 1614
Size: 710 530 374
Fragments arranged by size:
710 530 374
BsaAI yAC'GTr
Cuts at: 0 465 1614
Size: 465 1149
BsaBI GATnn'nnATC
Cuts at: 0 376 406 1614
Size: 376 30 1208
Fragments arranged by size:
1208 376 30
BsaHI Gr'CG_yC
Cuts at: 0 131 212 473 509 1614
Size: 131 81 261 36 1105
Fragments arranged by size:
1105 261 131 81 36
BsaWI w'CCGG_w
Cuts at: 0 719 1614
Size: 719 895
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 166 1614
Size: 166 1448
BseYI C'CCAG_C
Cuts at: 0 28 220 357 366 933 952 1614
Size: 28 192 137 9 567 19 662
Fragments arranged by size:
662 567 192 137 28 19 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 1614
Size: 550 1064
BsiEI CG_ry'CG
Cuts at: 0 54 1614
Size: 54 1560
Bsp1286I G_dGCh'C
Cuts at: 0 227 308 1614
Size: 227 81 1306
Fragments arranged by size:
1306 227 81
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 291 299 509 905 1614
Size: 291 8 210 396 709
Fragments arranged by size:
709 396 291 210 8
BsrBI CCG'CTC
Cuts at: 0 68 1614
Size: 68 1546
BsrDI GCAATG_nn'
Cuts at: 0 947 1313 1614
Size: 947 366 301
Fragments arranged by size:
947 366 301
BsrFI r'CCGG_y
Cuts at: 0 33 1614
Size: 33 1581
BssHII G'CGCG_C
Cuts at: 0 242 543 1614
Size: 242 301 1071
Fragments arranged by size:
1071 301 242
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1334 1614
Size: 1334 280
BstXI CCAn_nnnn'nTGG
Cuts at: 0 331 1137 1614
Size: 331 806 477
Fragments arranged by size:
806 477 331
BstYI r'GATC_y
Cuts at: 0 422 610 1182 1614
Size: 422 188 572 432
Fragments arranged by size:
572 432 422 188
Bsu36I CC'TnA_GG
Cuts at: 0 832 1614
Size: 832 782
BtgI C'CryG_G
Cuts at: 0 851 1078 1614
Size: 851 227 536
Fragments arranged by size:
851 536 227
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 677 1614
Size: 677 937
BtsI GCAGTG_nn'
Cuts at: 0 1328 1614
Size: 1328 286
DraIII CAC_nnn'GTG
Cuts at: 0 825 1614
Size: 825 789
EaeI y'GGCC_r
Cuts at: 0 51 770 1614
Size: 51 719 844
Fragments arranged by size:
844 719 51
EagI C'GGCC_G
Cuts at: 0 51 1614
Size: 51 1563
EarI CTCTTCn'nnn_
Cuts at: 0 1127 1240 1614
Size: 1127 113 374
Fragments arranged by size:
1127 374 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 457 635 1614
Size: 457 178 979
Fragments arranged by size:
979 457 178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 791 1221 1451 1614
Size: 367 424 430 230 163
Fragments arranged by size:
430 424 367 230 163
EcoNI CCTnn'n_nnAGG
Cuts at: 0 662 1614
Size: 662 952
EcoO109I rG'GnC_Cy
Cuts at: 0 1257 1614
Size: 1257 357
HaeII r_GCGC'y
Cuts at: 0 161 215 1614
Size: 161 54 1399
Fragments arranged by size:
1399 161 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 673 705 957 989 1614
Size: 673 32 252 32 625
Fragments arranged by size:
673 625 252 32 32
KasI G'GCGC_C
Cuts at: 0 211 1614
Size: 211 1403
KpnI G_GTAC'C
Cuts at: 0 1211 1614
Size: 1211 403
MfeI C'AATT_G
Cuts at: 0 1553 1614
Size: 1553 61
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 908 1614
Size: 908 706
MspA1I CmG'CkG
Cuts at: 0 63 933 1614
Size: 63 870 681
Fragments arranged by size:
870 681 63
NaeI GCC'GGC
Cuts at: 0 35 1614
Size: 35 1579
NarI GG'CG_CC
Cuts at: 0 212 1614
Size: 212 1402
NgoMIV G'CCGG_C
Cuts at: 0 33 1614
Size: 33 1581
NspI r_CATG'y
Cuts at: 0 582 1077 1614
Size: 582 495 537
Fragments arranged by size:
582 537 495
PfoI T'CCnGG_A
Cuts at: 0 1152 1614
Size: 1152 462
PpuMI rG'GwC_Cy
Cuts at: 0 1257 1614
Size: 1257 357
PstI C_TGCA'G
Cuts at: 0 208 1614
Size: 208 1406
PvuII CAG'CTG
Cuts at: 0 933 1614
Size: 933 681
SfcI C'TryA_G
Cuts at: 0 204 1614
Size: 204 1410
SfoI GGC'GCC
Cuts at: 0 213 1614
Size: 213 1401
SmaI CCC'GGG
Cuts at: 0 230 1614
Size: 230 1384
SmlI C'TyrA_G
Cuts at: 0 120 1614
Size: 120 1494
SphI G_CATG'C
Cuts at: 0 1077 1614
Size: 1077 537
StyI C'CwwG_G
Cuts at: 0 767 1614
Size: 767 847
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 529 620 1614
Size: 529 91 994
Fragments arranged by size:
994 529 91
TatI w'GTAC_w
Cuts at: 0 575 1284 1614
Size: 575 709 330
Fragments arranged by size:
709 575 330
XmaI C'CCGG_G
Cuts at: 0 228 1614
Size: 228 1386
Enzymes that do cut:
AarI Acc65I AclI AcuI AhdI AlfI AloI AlwNI
AvaI BamHI BanI BbeI BbsI BbvCI BciVI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI
KpnI MfeI MmeI MspA1I NaeI NarI NgoMIV NspI
PfoI PpuMI PstI PvuII SfcI SfoI SmaI SmlI
SphI StyI TaqII TatI XmaI
Enzymes that do not cut:
AatII AccI AfeI AflII AflIII AgeI AleI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanII
BcgI BclI BfrBI BglI BmgBI BmtI BsaXI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrGI BssSI BstBI
BstEII BstZ17I ClaI CspCI DraI DrdI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
MluI MscI MslI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
TstI Tth111I XbaI XcmI XhoI XmnI ZraI