Restriction map for
pFN21AB5918
(Linear) (Six-base) MAPSORT of: KIBB5918.seq Check: 7271 from: 1 to: 1596
KIBB5918.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1093 1596
Size: 1093 503
AflIII A'CryG_T
Cuts at: 0 865 1596
Size: 865 731
AleI CACnn'nnGTG
Cuts at: 0 442 1596
Size: 442 1154
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 709 741 1596
Size: 709 32 855
Fragments arranged by size:
855 709 32
AlwNI CAG_nnn'CTG
Cuts at: 0 70 861 1047 1068 1336 1596
Size: 70 791 186 21 268 260
Fragments arranged by size:
791 268 260 186 70 21
ApaI G_GGCC'C
Cuts at: 0 1054 1062 1596
Size: 1054 8 534
Fragments arranged by size:
1054 534 8
ApoI r'AATT_y
Cuts at: 0 1233 1596
Size: 1233 363
AvaI C'yCGr_G
Cuts at: 0 49 116 425 671 1053 1160 1279 1343
Size: 49 67 309 246 382 107 119 64
Cuts at: 1343 1596
Size: 253
Fragments arranged by size:
382 309 253 246 119 107 67 64
49
BanI G'GyrC_C
Cuts at: 0 354 420 850 933 1357 1596
Size: 354 66 430 83 424 239
Fragments arranged by size:
430 424 354 239 83 66
BanII G_rGCy'C
Cuts at: 0 57 497 1054 1062 1286 1596
Size: 57 440 557 8 224 310
Fragments arranged by size:
557 440 310 224 57 8
BbeI G_GCGC'C
Cuts at: 0 937 1596
Size: 937 659
BbvCI CC'TCA_GC
Cuts at: 0 500 1492 1596
Size: 500 992 104
Fragments arranged by size:
992 500 104
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 498 532 1596
Size: 498 34 1064
Fragments arranged by size:
1064 498 34
BglI GCCn_nnn'nGGC
Cuts at: 0 585 1596
Size: 585 1011
BglII A'GATC_T
Cuts at: 0 1347 1596
Size: 1347 249
Bme1580I G_kGCm'C
Cuts at: 0 423 853 1054 1062 1360 1483 1596
Size: 423 430 201 8 298 123 113
Fragments arranged by size:
430 423 298 201 123 113 8
BmgBI CAC'GTC
Cuts at: 0 866 1223 1596
Size: 866 357 373
Fragments arranged by size:
866 373 357
BmrI ACTGGGnnnn_n'
Cuts at: 0 724 769 889 1057 1596
Size: 724 45 120 168 539
Fragments arranged by size:
724 539 168 120 45
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 624 1596
Size: 624 972
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 610 1144 1596
Size: 320 290 534 452
Fragments arranged by size:
534 452 320 290
Bpu10I CC'TnA_GC
Cuts at: 0 500 1492 1596
Size: 500 992 104
Fragments arranged by size:
992 500 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1270 1370 1372 1596
Size: 1270 100 2 224
Fragments arranged by size:
1270 224 100 2
BsaI GGTCTCn'nnnn_
Cuts at: 0 175 969 1596
Size: 175 794 627
Fragments arranged by size:
794 627 175
BsaAI yAC'GTr
Cuts at: 0 1499 1596
Size: 1499 97
BsaBI GATnn'nnATC
Cuts at: 0 1444 1596
Size: 1444 152
BsaHI Gr'CG_yC
Cuts at: 0 934 1596
Size: 934 662
BsaWI w'CCGG_w
Cuts at: 0 224 1596
Size: 224 1372
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 113 143 1596
Size: 113 30 1453
Fragments arranged by size:
1453 113 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 540 1596
Size: 540 1056
BseYI C'CCAG_C
Cuts at: 0 7 854 1040 1061 1238 1596
Size: 7 847 186 21 177 358
Fragments arranged by size:
847 358 186 177 21 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 890 1596
Size: 890 706
BsiHKAI G_wGCw'C
Cuts at: 0 1286 1384 1424 1596
Size: 1286 98 40 172
Fragments arranged by size:
1286 172 98 40
Bsp1286I G_dGCh'C
Cuts at: 0 57 423 497 853 1054 1062 1286 1360
Size: 57 366 74 356 201 8 224 74
Cuts at: 1360 1384 1424 1483 1596
Size: 24 40 59 113
Fragments arranged by size:
366 356 224 201 113 74 74 59
57 40 24 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 581 1596
Size: 581 1015
BsrDI GCAATG_nn'
Cuts at: 0 81 252 1273 1596
Size: 81 171 1021 323
Fragments arranged by size:
1021 323 171 81
BsrFI r'CCGG_y
Cuts at: 0 1132 1596
Size: 1132 464
BssSI C'ACGA_G
Cuts at: 0 552 1596
Size: 552 1044
BstEII G'GTnAC_C
Cuts at: 0 1395 1407 1596
Size: 1395 12 189
Fragments arranged by size:
1395 189 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 171 519 1596
Size: 171 348 1077
Fragments arranged by size:
1077 348 171
BstYI r'GATC_y
Cuts at: 0 1347 1596
Size: 1347 249
BtgI C'CryG_G
Cuts at: 0 440 453 512 537 701 1596
Size: 440 13 59 25 164 895
Fragments arranged by size:
895 440 164 59 25 13
BtsI GCAGTG_nn'
Cuts at: 0 901 1312 1481 1596
Size: 901 411 169 115
Fragments arranged by size:
901 411 169 115
DrdI GACnn_nn'nnGTC
Cuts at: 0 90 1596
Size: 90 1506
EaeI y'GGCC_r
Cuts at: 0 812 1130 1134 1225 1596
Size: 812 318 4 91 371
Fragments arranged by size:
812 371 318 91 4
EcoICRI GAG'CTC
Cuts at: 0 1284 1596
Size: 1284 312
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 610 1093 1144 1596
Size: 320 290 483 51 452
Fragments arranged by size:
483 452 320 290 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1072 1596
Size: 1072 524
EcoO109I rG'GnC_Cy
Cuts at: 0 67 675 1058 1559 1596
Size: 67 608 383 501 37
Fragments arranged by size:
608 501 383 67 37
FseI GG_CCGG'CC
Cuts at: 0 1136 1596
Size: 1136 460
HaeII r_GCGC'y
Cuts at: 0 937 1596
Size: 937 659
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 283 315 516 548 793 825 1191 1223
Size: 283 32 201 32 245 32 366 32
Cuts at: 1223 1326 1358 1596
Size: 103 32 238
Fragments arranged by size:
366 283 245 238 201 103 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 302 1596
Size: 302 1294
KasI G'GCGC_C
Cuts at: 0 933 1596
Size: 933 663
MscI TGG'CCA
Cuts at: 0 1227 1596
Size: 1227 369
MslI CAynn'nnrTG
Cuts at: 0 442 1453 1596
Size: 442 1011 143
Fragments arranged by size:
1011 442 143
MspA1I CmG'CkG
Cuts at: 0 484 499 858 1518 1596
Size: 484 15 359 660 78
Fragments arranged by size:
660 484 359 78 15
NaeI GCC'GGC
Cuts at: 0 1134 1596
Size: 1134 462
NarI GG'CG_CC
Cuts at: 0 934 1596
Size: 934 662
NcoI C'CATG_G
Cuts at: 0 537 1596
Size: 537 1059
NgoMIV G'CCGG_C
Cuts at: 0 1132 1596
Size: 1132 464
NspI r_CATG'y
Cuts at: 0 143 1596
Size: 143 1453
PflMI CCAn_nnn'nTGG
Cuts at: 0 70 588 1047 1596
Size: 70 518 459 549
Fragments arranged by size:
549 518 459 70
PfoI T'CCnGG_A
Cuts at: 0 1209 1596
Size: 1209 387
PpuMI rG'GwC_Cy
Cuts at: 0 67 675 1559 1596
Size: 67 608 884 37
Fragments arranged by size:
884 608 67 37
PshAI GACnn'nnGTC
Cuts at: 0 634 703 1596
Size: 634 69 893
Fragments arranged by size:
893 634 69
PspOMI G'GGCC_C
Cuts at: 0 1050 1058 1596
Size: 1050 8 538
Fragments arranged by size:
1050 538 8
PstI C_TGCA'G
Cuts at: 0 908 1488 1596
Size: 908 580 108
Fragments arranged by size:
908 580 108
PvuII CAG'CTG
Cuts at: 0 484 1596
Size: 484 1112
SacI G_AGCT'C
Cuts at: 0 1286 1596
Size: 1286 310
SanDI GG'GwC_CC
Cuts at: 0 1559 1596
Size: 1559 37
SfcI C'TryA_G
Cuts at: 0 904 921 1484 1596
Size: 904 17 563 112
Fragments arranged by size:
904 563 112 17
SfoI GGC'GCC
Cuts at: 0 935 1596
Size: 935 661
SmaI CCC'GGG
Cuts at: 0 118 427 673 1162 1596
Size: 118 309 246 489 434
Fragments arranged by size:
489 434 309 246 118
SmlI C'TyrA_G
Cuts at: 0 1285 1343 1351 1385 1596
Size: 1285 58 8 34 211
Fragments arranged by size:
1285 211 58 34 8
StuI AGG'CCT
Cuts at: 0 820 1490 1596
Size: 820 670 106
Fragments arranged by size:
820 670 106
StyI C'CwwG_G
Cuts at: 0 537 582 815 1013 1596
Size: 537 45 233 198 583
Fragments arranged by size:
583 537 233 198 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 868 991 1596
Size: 868 123 605
Fragments arranged by size:
868 605 123
TatI w'GTAC_w
Cuts at: 0 326 1596
Size: 326 1270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 26 58 113 145 1596
Size: 26 32 55 32 1451
Fragments arranged by size:
1451 55 32 32 26
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 412 438 1596
Size: 412 26 1158
Fragments arranged by size:
1158 412 26
XhoI C'TCGA_G
Cuts at: 0 1343 1596
Size: 1343 253
XmaI C'CCGG_G
Cuts at: 0 116 425 671 1160 1596
Size: 116 309 246 489 436
Fragments arranged by size:
489 436 309 246 116
Enzymes that do cut:
AcuI AflIII AleI AloI AlwNI ApaI ApoI AvaI
BanI BanII BbeI BbvCI BcgI BglI BglII Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrDI BsrFI BssSI BstEII BstXI BstYI
BtgI BtsI DrdI EaeI EcoICRI Eco57MI EcoNI EcoO109I
FseI HaeII Hin4I HincII KasI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PflMI PfoI PpuMI
PshAI PspOMI PstI PvuII SacI SanDI SfcI SfoI
SmaI SmlI StuI StyI TaqII TatI TstI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BciVI BclI BfrBI BlpI BmtI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII EagI EarI EciI EcoRI EcoRV FalI FspI
FspAI HindIII HpaI KpnI MfeI MluI MmeI NdeI
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XmnI ZraI