Restriction map for
pFN21AB5904
(Linear) (Six-base) MAPSORT of: KIBB5904.seq Check: 5800 from: 1 to: 1575
KIBB5904.orf
With 182 enzymes: SgfI *
January 30, 2018 10:51 ..
AatII G_ACGT'C
Cuts at: 0 288 1575
Size: 288 1287
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 1575
Size: 380 1195
AfeI AGC'GCT
Cuts at: 0 457 1575
Size: 457 1118
AflII C'TTAA_G
Cuts at: 0 1311 1575
Size: 1311 264
AleI CACnn'nnGTG
Cuts at: 0 794 1575
Size: 794 781
AlwNI CAG_nnn'CTG
Cuts at: 0 1501 1575
Size: 1501 74
ApaI G_GGCC'C
Cuts at: 0 1011 1575
Size: 1011 564
BanII G_rGCy'C
Cuts at: 0 1011 1575
Size: 1011 564
BbsI GAAGACnn'nnnn_
Cuts at: 0 720 1575
Size: 720 855
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 529 563 1575
Size: 529 34 1012
Fragments arranged by size:
1012 529 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 441 1575
Size: 441 1134
BclI T'GATC_A
Cuts at: 0 29 110 1575
Size: 29 81 1465
Fragments arranged by size:
1465 81 29
Bme1580I G_kGCm'C
Cuts at: 0 1011 1310 1575
Size: 1011 299 265
Fragments arranged by size:
1011 299 265
BmrI ACTGGGnnnn_n'
Cuts at: 0 801 1575
Size: 801 774
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 884 1575
Size: 594 290 691
Fragments arranged by size:
691 594 290
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 957 1575
Size: 505 452 618
Fragments arranged by size:
618 505 452
BsaBI GATnn'nnATC
Cuts at: 0 857 1575
Size: 857 718
BsaHI Gr'CG_yC
Cuts at: 0 285 1575
Size: 285 1290
BsaWI w'CCGG_w
Cuts at: 0 423 1575
Size: 423 1152
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 810 840 1575
Size: 810 30 735
Fragments arranged by size:
810 735 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 514 575 887 938 1376 1575
Size: 514 61 312 51 438 199
Fragments arranged by size:
514 438 312 199 61 51
BseYI C'CCAG_C
Cuts at: 0 1432 1575
Size: 1432 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 1575
Size: 360 1215
Bsp1286I G_dGCh'C
Cuts at: 0 1011 1310 1575
Size: 1011 299 265
Fragments arranged by size:
1011 299 265
BspHI T'CATG_A
Cuts at: 0 1113 1575
Size: 1113 462
BsrDI GCAATG_nn'
Cuts at: 0 11 34 1350 1441 1575
Size: 11 23 1316 91 134
Fragments arranged by size:
1316 134 91 23 11
BstAPI GCAn_nnn'nTGC
Cuts at: 0 13 1575
Size: 13 1562
BstXI CCAn_nnnn'nTGG
Cuts at: 0 796 1159 1174 1189 1204 1240 1575
Size: 796 363 15 15 15 36 335
Fragments arranged by size:
796 363 335 36 15 15 15
BstYI r'GATC_y
Cuts at: 0 844 1366 1575
Size: 844 522 209
Fragments arranged by size:
844 522 209
Bsu36I CC'TnA_GG
Cuts at: 0 737 1418 1575
Size: 737 681 157
Fragments arranged by size:
737 681 157
BtgI C'CryG_G
Cuts at: 0 840 1083 1143 1158 1188 1203 1284 1290
Size: 840 243 60 15 30 15 81 6
Cuts at: 1290 1575
Size: 285
Fragments arranged by size:
840 285 243 81 60 30 15 15
6
BtsI GCAGTG_nn'
Cuts at: 0 19 78 1218 1575
Size: 19 59 1140 357
Fragments arranged by size:
1140 357 59 19
DrdI GACnn_nn'nnGTC
Cuts at: 0 235 1575
Size: 235 1340
EaeI y'GGCC_r
Cuts at: 0 404 1287 1575
Size: 404 883 288
Fragments arranged by size:
883 404 288
EarI CTCTTCn'nnn_
Cuts at: 0 399 1575
Size: 399 1176
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 594 884 1575
Size: 380 214 290 691
Fragments arranged by size:
691 380 290 214
EcoO109I rG'GnC_Cy
Cuts at: 0 351 943 1007 1336 1575
Size: 351 592 64 329 239
Fragments arranged by size:
592 351 329 239 64
HaeII r_GCGC'y
Cuts at: 0 459 1575
Size: 459 1116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 502 534 1575
Size: 502 32 1041
Fragments arranged by size:
1041 502 32
HincII GTy'rAC
Cuts at: 0 982 1575
Size: 982 593
MfeI C'AATT_G
Cuts at: 0 145 1575
Size: 145 1430
MscI TGG'CCA
Cuts at: 0 406 1289 1575
Size: 406 883 286
Fragments arranged by size:
883 406 286
MslI CAynn'nnrTG
Cuts at: 0 338 635 794 881 920 1067 1097 1112
Size: 338 297 159 87 39 147 30 15
Cuts at: 1112 1118 1142 1157 1172 1187 1202 1223 1575
Size: 6 24 15 15 15 15 21 352
Fragments arranged by size:
352 338 297 159 147 87 39 30
24 21 15 15 15 15 15 6
NcoI C'CATG_G
Cuts at: 0 840 1143 1158 1188 1203 1284 1575
Size: 840 303 15 30 15 81 291
Fragments arranged by size:
840 303 291 81 30 15 15
NspI r_CATG'y
Cuts at: 0 988 1575
Size: 988 587
PflMI CCAn_nnn'nTGG
Cuts at: 0 1303 1575
Size: 1303 272
PfoI T'CCnGG_A
Cuts at: 0 244 1575
Size: 244 1331
PpuMI rG'GwC_Cy
Cuts at: 0 351 1336 1575
Size: 351 985 239
Fragments arranged by size:
985 351 239
PspOMI G'GGCC_C
Cuts at: 0 1007 1575
Size: 1007 568
PstI C_TGCA'G
Cuts at: 0 73 655 1575
Size: 73 582 920
Fragments arranged by size:
920 582 73
SfcI C'TryA_G
Cuts at: 0 69 459 651 1575
Size: 69 390 192 924
Fragments arranged by size:
924 390 192 69
SmlI C'TyrA_G
Cuts at: 0 484 972 1311 1575
Size: 484 488 339 264
Fragments arranged by size:
488 484 339 264
StyI C'CwwG_G
Cuts at: 0 683 840 1143 1158 1188 1203 1269 1284
Size: 683 157 303 15 30 15 66 15
Cuts at: 1284 1575
Size: 291
Fragments arranged by size:
683 303 291 157 66 30 15 15
15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 225 548 1575
Size: 225 323 1027
Fragments arranged by size:
1027 323 225
TatI w'GTAC_w
Cuts at: 0 491 1575
Size: 491 1084
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 249 281 810 842 1575
Size: 249 32 529 32 733
Fragments arranged by size:
733 529 249 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1340 1575
Size: 1340 235
XbaI T'CTAG_A
Cuts at: 0 102 847 1575
Size: 102 745 728
Fragments arranged by size:
745 728 102
ZraI GAC'GTC
Cuts at: 0 286 1575
Size: 286 1289
Enzymes that do cut:
AatII AcuI AfeI AflII AleI AlwNI ApaI BanII
BbsI BcgI BciVI BclI Bme1580I BmrI BpmI BpuEI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI Bsp1286I
BspHI BsrDI BstAPI BstXI BstYI Bsu36I BtgI BtsI
DrdI EaeI EarI Eco57MI EcoO109I HaeII Hin4I HincII
MfeI MscI MslI NcoI NspI PflMI PfoI PpuMI
PspOMI PstI SfcI SmlI StyI TaqII TatI TstI
Tth111I XbaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflIII AgeI AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BfrBI BglI BglII
BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI DraIII EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MluI MmeI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI XcmI XhoI XmaI
XmnI