Restriction map for
pFN21AB5903
(Linear) (Six-base) MAPSORT of: KIBB5903.seq Check: 549 from: 1 to: 1503
KIBB5903.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 971 1253 1503
Size: 971 282 250
Fragments arranged by size:
971 282 250
AleI CACnn'nnGTG
Cuts at: 0 223 1503
Size: 223 1280
ApoI r'AATT_y
Cuts at: 0 19 1503
Size: 19 1484
AvaI C'yCGr_G
Cuts at: 0 63 323 466 1503
Size: 63 260 143 1037
Fragments arranged by size:
1037 260 143 63
BanII G_rGCy'C
Cuts at: 0 508 1503
Size: 508 995
BbsI GAAGACnn'nnnn_
Cuts at: 0 1235 1503
Size: 1235 268
BbvCI CC'TCA_GC
Cuts at: 0 98 1503
Size: 98 1405
BglI GCCn_nnn'nGGC
Cuts at: 0 81 470 1503
Size: 81 389 1033
Fragments arranged by size:
1033 389 81
Bme1580I G_kGCm'C
Cuts at: 0 116 466 1503
Size: 116 350 1037
Fragments arranged by size:
1037 350 116
BmgBI CAC'GTC
Cuts at: 0 348 1503
Size: 348 1155
BmrI ACTGGGnnnn_n'
Cuts at: 0 926 1023 1503
Size: 926 97 480
Fragments arranged by size:
926 480 97
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1454 1503
Size: 1454 49
Bpu10I CC'TnA_GC
Cuts at: 0 98 1503
Size: 98 1405
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 1269 1503
Size: 217 1052 234
Fragments arranged by size:
1052 234 217
BsaI GGTCTCn'nnnn_
Cuts at: 0 568 675 1503
Size: 568 107 828
Fragments arranged by size:
828 568 107
BsaAI yAC'GTr
Cuts at: 0 816 1503
Size: 816 687
BsaHI Gr'CG_yC
Cuts at: 0 295 518 1503
Size: 295 223 985
Fragments arranged by size:
985 295 223
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 655 685 1503
Size: 655 30 818
Fragments arranged by size:
818 655 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1451 1503
Size: 1451 52
BseYI C'CCAG_C
Cuts at: 0 554 851 1503
Size: 554 297 652
Fragments arranged by size:
652 554 297
BsiEI CG_ry'CG
Cuts at: 0 153 1503
Size: 153 1350
BsiHKAI G_wGCw'C
Cuts at: 0 596 1503
Size: 596 907
BsmI GAATG_Cn'
Cuts at: 0 1165 1503
Size: 1165 338
BsmBI CGTCTCn'nnnn_
Cuts at: 0 409 1503
Size: 409 1094
Bsp1286I G_dGCh'C
Cuts at: 0 116 466 508 596 1503
Size: 116 350 42 88 907
Fragments arranged by size:
907 350 116 88 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 659 1503
Size: 659 844
BsrDI GCAATG_nn'
Cuts at: 0 165 1503
Size: 165 1338
BsrFI r'CCGG_y
Cuts at: 0 152 1503
Size: 152 1351
BsrGI T'GTAC_A
Cuts at: 0 162 1503
Size: 162 1341
BssHII G'CGCG_C
Cuts at: 0 567 1503
Size: 567 936
BstAPI GCAn_nnn'nTGC
Cuts at: 0 111 1503
Size: 111 1392
BstXI CCAn_nnnn'nTGG
Cuts at: 0 225 1503
Size: 225 1278
Bsu36I CC'TnA_GG
Cuts at: 0 716 1503
Size: 716 787
BtgI C'CryG_G
Cuts at: 0 275 1503
Size: 275 1228
ClaI AT'CG_AT
Cuts at: 0 821 1503
Size: 821 682
DraI TTT'AAA
Cuts at: 0 1014 1503
Size: 1014 489
DraIII CAC_nnn'GTG
Cuts at: 0 600 1503
Size: 600 903
DrdI GACnn_nn'nnGTC
Cuts at: 0 51 1503
Size: 51 1452
EaeI y'GGCC_r
Cuts at: 0 150 371 1503
Size: 150 221 1132
Fragments arranged by size:
1132 221 150
EagI C'GGCC_G
Cuts at: 0 150 1503
Size: 150 1353
EarI CTCTTCn'nnn_
Cuts at: 0 217 256 409 487 493 995 1218 1503
Size: 217 39 153 78 6 502 223 285
Fragments arranged by size:
502 285 223 217 153 78 39 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 971 1253 1454 1503
Size: 971 282 201 49
Fragments arranged by size:
971 282 201 49
EcoO109I rG'GnC_Cy
Cuts at: 0 93 1503
Size: 93 1410
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1477 1503
Size: 1477 26
FspI TGC'GCA
Cuts at: 0 655 1503
Size: 655 848
HaeII r_GCGC'y
Cuts at: 0 243 1503
Size: 243 1260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 912 944 1503
Size: 912 32 559
Fragments arranged by size:
912 559 32
HindIII A'AGCT_T
Cuts at: 0 1417 1503
Size: 1417 86
MscI TGG'CCA
Cuts at: 0 373 1503
Size: 373 1130
MslI CAynn'nnrTG
Cuts at: 0 223 547 631 1369 1503
Size: 223 324 84 738 134
Fragments arranged by size:
738 324 223 134 84
MspA1I CmG'CkG
Cuts at: 0 59 132 1503
Size: 59 73 1371
Fragments arranged by size:
1371 73 59
NspI r_CATG'y
Cuts at: 0 540 864 1503
Size: 540 324 639
Fragments arranged by size:
639 540 324
PasI CC'CwG_GG
Cuts at: 0 78 1503
Size: 78 1425
PflMI CCAn_nnn'nTGG
Cuts at: 0 305 1398 1503
Size: 305 1093 105
Fragments arranged by size:
1093 305 105
PmlI CAC'GTG
Cuts at: 0 816 1503
Size: 816 687
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 709 741 1242 1274 1503
Size: 709 32 501 32 229
Fragments arranged by size:
709 501 229 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 93 1503
Size: 93 1410
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 930 962 1503
Size: 930 32 541
Fragments arranged by size:
930 541 32
PstI C_TGCA'G
Cuts at: 0 1211 1503
Size: 1211 292
SfcI C'TryA_G
Cuts at: 0 1207 1281 1503
Size: 1207 74 222
Fragments arranged by size:
1207 222 74
SmlI C'TyrA_G
Cuts at: 0 232 1248 1503
Size: 232 1016 255
Fragments arranged by size:
1016 255 232
SphI G_CATG'C
Cuts at: 0 864 1503
Size: 864 639
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 297 1503
Size: 297 1206
TatI w'GTAC_w
Cuts at: 0 162 766 1503
Size: 162 604 737
Fragments arranged by size:
737 604 162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 220 252 655 687 1503
Size: 220 32 403 32 816
Fragments arranged by size:
816 403 220 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 250 1091 1232 1320 1503
Size: 250 841 141 88 183
Fragments arranged by size:
841 250 183 141 88
Enzymes that do cut:
AcuI AleI ApoI AvaI BanII BbsI BbvCI BglI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrDI BsrFI BsrGI BssHII BstAPI BstXI
Bsu36I BtgI ClaI DraI DraIII DrdI EaeI EagI
EarI Eco57MI EcoO109I FalI FspI HaeII Hin4I HindIII
MscI MslI MspA1I NspI PasI PflMI PmlI PpiI
PpuMI PsrI PstI SfcI SmlI SphI TaqII TatI
TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BcgI
BciVI BclI BfrBI BglII BlpI BmtI BplI BsaBI
BsaWI BsgI BsiWI BspEI BspHI BsrBI BssSI BstBI
BstEII BstYI BstZ17I BtgZI BtsI CspCI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspAI HincII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PfoI
PmeI PshAI PsiI PspOMI PspXI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI
XmaI ZraI