Restriction map for
pFN21AB5889
(Linear) (Six-base) MAPSORT of: KIBB5889.seq Check: 7430 from: 1 to: 1479
KIBB5889.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AatII G_ACGT'C
Cuts at: 0 872 1479
Size: 872 607
AccI GT'mk_AC
Cuts at: 0 872 1479
Size: 872 607
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 1479
Size: 400 1079
AfeI AGC'GCT
Cuts at: 0 943 1479
Size: 943 536
AflIII A'CryG_T
Cuts at: 0 1184 1479
Size: 1184 295
AleI CACnn'nnGTG
Cuts at: 0 1187 1479
Size: 1187 292
ApaI G_GGCC'C
Cuts at: 0 566 1479
Size: 566 913
ApaLI G'TGCA_C
Cuts at: 0 997 1075 1234 1375 1479
Size: 997 78 159 141 104
Fragments arranged by size:
997 159 141 104 78
AvaI C'yCGr_G
Cuts at: 0 62 663 810 1446 1479
Size: 62 601 147 636 33
Fragments arranged by size:
636 601 147 62 33
BanI G'GyrC_C
Cuts at: 0 201 393 423 690 754 1479
Size: 201 192 30 267 64 725
Fragments arranged by size:
725 267 201 192 64 30
BanII G_rGCy'C
Cuts at: 0 143 239 566 1207 1268 1331 1479
Size: 143 96 327 641 61 63 148
Fragments arranged by size:
641 327 148 143 96 63 61
BbeI G_GCGC'C
Cuts at: 0 205 397 427 1479
Size: 205 192 30 1052
Fragments arranged by size:
1052 205 192 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 553 1479
Size: 553 926
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 672 706 1479
Size: 672 34 773
Fragments arranged by size:
773 672 34
BglI GCCn_nnn'nGGC
Cuts at: 0 199 229 475 778 1342 1399 1479
Size: 199 30 246 303 564 57 80
Fragments arranged by size:
564 303 246 199 80 57 30
BlpI GC'TnA_GC
Cuts at: 0 137 721 1201 1479
Size: 137 584 480 278
Fragments arranged by size:
584 480 278 137
Bme1580I G_kGCm'C
Cuts at: 0 566 695 716 928 1001 1079 1238 1379
Size: 566 129 21 212 73 78 159 141
Cuts at: 1379 1479
Size: 100
Fragments arranged by size:
566 212 159 141 129 100 78 73
21
BmgBI CAC'GTC
Cuts at: 0 1065 1479
Size: 1065 414
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 226 1315 1479
Size: 127 99 1089 164
Fragments arranged by size:
1089 164 127 99
Bpu10I CC'TnA_GC
Cuts at: 0 697 706 1479
Size: 697 9 773
Fragments arranged by size:
773 697 9
BsaAI yAC'GTr
Cuts at: 0 975 1185 1479
Size: 975 210 294
Fragments arranged by size:
975 294 210
BsaHI Gr'CG_yC
Cuts at: 0 202 394 424 869 1454 1479
Size: 202 192 30 445 585 25
Fragments arranged by size:
585 445 202 192 30 25
BsaWI w'CCGG_w
Cuts at: 0 614 824 1479
Size: 614 210 655
Fragments arranged by size:
655 614 210
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1000 1030 1479
Size: 1000 30 449
Fragments arranged by size:
1000 449 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1180 1479
Size: 1180 299
BseYI C'CCAG_C
Cuts at: 0 48 716 1394 1460 1479
Size: 48 668 678 66 19
Fragments arranged by size:
678 668 66 48 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1389 1479
Size: 1389 90
BsiEI CG_ry'CG
Cuts at: 0 233 302 434 512 527 654 806 1045
Size: 233 69 132 78 15 127 152 239
Cuts at: 1045 1479
Size: 434
Fragments arranged by size:
434 239 233 152 132 127 78 69
15
BsiHKAI G_wGCw'C
Cuts at: 0 143 266 1001 1079 1207 1238 1268 1379
Size: 143 123 735 78 128 31 30 111
Cuts at: 1379 1479
Size: 100
Fragments arranged by size:
735 143 128 123 111 100 78 31
30
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1445 1479
Size: 1445 34
Bsp1286I G_dGCh'C
Cuts at: 0 143 239 266 566 695 716 928 1001
Size: 143 96 27 300 129 21 212 73
Cuts at: 1001 1079 1207 1238 1268 1331 1379 1479
Size: 78 128 31 30 63 48 100
Fragments arranged by size:
300 212 143 129 128 100 96 78
73 63 48 31 30 27 21
BspEI T'CCGG_A
Cuts at: 0 614 824 1479
Size: 614 210 655
Fragments arranged by size:
655 614 210
BspHI T'CATG_A
Cuts at: 0 80 1479
Size: 80 1399
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 117 1307 1479
Size: 117 1190 172
Fragments arranged by size:
1190 172 117
BsrBI CCG'CTC
Cuts at: 0 725 1479
Size: 725 754
BsrFI r'CCGG_y
Cuts at: 0 198 649 730 757 784 1402 1479
Size: 198 451 81 27 27 618 77
Fragments arranged by size:
618 451 198 81 77 27 27
BssSI C'ACGA_G
Cuts at: 0 1162 1479
Size: 1162 317
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1358 1367 1479
Size: 1358 9 112
Fragments arranged by size:
1358 112 9
BstEII G'GTnAC_C
Cuts at: 0 795 1479
Size: 795 684
Bsu36I CC'TnA_GG
Cuts at: 0 101 1479
Size: 101 1378
BtgI C'CryG_G
Cuts at: 0 287 428 470 515 686 1286 1323 1339
Size: 287 141 42 45 171 600 37 16
Cuts at: 1339 1479
Size: 140
Fragments arranged by size:
600 287 171 141 140 45 42 37
16
BtsI GCAGTG_nn'
Cuts at: 0 782 1377 1479
Size: 782 595 102
Fragments arranged by size:
782 595 102
DraIII CAC_nnn'GTG
Cuts at: 0 1314 1479
Size: 1314 165
EaeI y'GGCC_r
Cuts at: 0 230 299 431 479 651 779 1042 1400
Size: 230 69 132 48 172 128 263 358
Cuts at: 1400 1404 1479
Size: 4 75
Fragments arranged by size:
358 263 230 172 132 128 75 69
48 4
EagI C'GGCC_G
Cuts at: 0 230 299 431 651 1042 1479
Size: 230 69 132 220 391 437
Fragments arranged by size:
437 391 230 220 132 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 273 392 444 1479
Size: 273 119 52 1035
Fragments arranged by size:
1035 273 119 52
EcoICRI GAG'CTC
Cuts at: 0 141 1205 1266 1479
Size: 141 1064 61 213
Fragments arranged by size:
1064 213 141 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 226 400 1315 1479
Size: 127 99 174 915 164
Fragments arranged by size:
915 174 164 127 99
EcoO109I rG'GnC_Cy
Cuts at: 0 222 562 563 657 1246 1335 1479
Size: 222 340 1 94 589 89 144
Fragments arranged by size:
589 340 222 144 94 89 1
FseI GG_CCGG'CC
Cuts at: 0 653 1406 1479
Size: 653 753 73
Fragments arranged by size:
753 653 73
FspI TGC'GCA
Cuts at: 0 1303 1479
Size: 1303 176
HaeII r_GCGC'y
Cuts at: 0 205 397 427 770 945 995 1479
Size: 205 192 30 343 175 50 484
Fragments arranged by size:
484 343 205 192 175 50 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 1479
Size: 130 32 1317
Fragments arranged by size:
1317 130 32
KasI G'GCGC_C
Cuts at: 0 201 393 423 1479
Size: 201 192 30 1056
Fragments arranged by size:
1056 201 192 30
MslI CAynn'nnrTG
Cuts at: 0 1187 1231 1259 1479
Size: 1187 44 28 220
Fragments arranged by size:
1187 220 44 28
MspA1I CmG'CkG
Cuts at: 0 289 304 313 373 430 472 720 1341
Size: 289 15 9 60 57 42 248 621
Cuts at: 1341 1398 1479
Size: 57 81
Fragments arranged by size:
621 289 248 81 60 57 57 42
15 9
NaeI GCC'GGC
Cuts at: 0 200 651 732 759 1404 1479
Size: 200 451 81 27 645 75
Fragments arranged by size:
645 451 200 81 75 27
NarI GG'CG_CC
Cuts at: 0 202 394 424 1479
Size: 202 192 30 1055
Fragments arranged by size:
1055 202 192 30
NcoI C'CATG_G
Cuts at: 0 686 1479
Size: 686 793
NgoMIV G'CCGG_C
Cuts at: 0 198 649 730 757 1402 1479
Size: 198 451 81 27 645 77
Fragments arranged by size:
645 451 198 81 77 27
NotI GC'GGCC_GC
Cuts at: 0 299 1479
Size: 299 1180
NspI r_CATG'y
Cuts at: 0 1413 1479
Size: 1413 66
PasI CC'CwG_GG
Cuts at: 0 165 1479
Size: 165 1314
PmlI CAC'GTG
Cuts at: 0 975 1185 1479
Size: 975 210 294
Fragments arranged by size:
975 294 210
PpuMI rG'GwC_Cy
Cuts at: 0 657 1246 1479
Size: 657 589 233
Fragments arranged by size:
657 589 233
PspOMI G'GGCC_C
Cuts at: 0 562 1479
Size: 562 917
PstI C_TGCA'G
Cuts at: 0 128 1479
Size: 128 1351
PvuII CAG'CTG
Cuts at: 0 720 1398 1479
Size: 720 678 81
Fragments arranged by size:
720 678 81
SacI G_AGCT'C
Cuts at: 0 143 1207 1268 1479
Size: 143 1064 61 211
Fragments arranged by size:
1064 211 143 61
SacII CC_GC'GG
Cuts at: 0 290 431 473 1342 1479
Size: 290 141 42 869 137
Fragments arranged by size:
869 290 141 137 42
SanDI GG'GwC_CC
Cuts at: 0 657 1479
Size: 657 822
SbfI CC_TGCA'GG
Cuts at: 0 128 1479
Size: 128 1351
SfcI C'TryA_G
Cuts at: 0 124 1479
Size: 124 1355
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 229 1479
Size: 229 1250
SfoI GGC'GCC
Cuts at: 0 203 395 425 1479
Size: 203 192 30 1054
Fragments arranged by size:
1054 203 192 30
SgrAI Cr'CCGG_yG
Cuts at: 0 198 784 1479
Size: 198 586 695
Fragments arranged by size:
695 586 198
SmaI CCC'GGG
Cuts at: 0 64 1448 1479
Size: 64 1384 31
Fragments arranged by size:
1384 64 31
StuI AGG'CCT
Cuts at: 0 859 1479
Size: 859 620
StyI C'CwwG_G
Cuts at: 0 536 587 644 686 1479
Size: 536 51 57 42 793
Fragments arranged by size:
793 536 57 51 42
XmaI C'CCGG_G
Cuts at: 0 62 1446 1479
Size: 62 1384 33
Fragments arranged by size:
1384 62 33
XmnI GAAnn'nnTTC
Cuts at: 0 88 1479
Size: 88 1391
ZraI GAC'GTC
Cuts at: 0 870 1479
Size: 870 609
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII AleI ApaI ApaLI
AvaI BanI BanII BbeI BbsI BcgI BglI BlpI
Bme1580I BmgBI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrFI BssSI BstAPI BstEII Bsu36I
BtgI BtsI DraIII EaeI EagI EciI EcoICRI Eco57MI
EcoO109I FseI FspI HaeII Hin4I KasI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PasI PmlI
PpuMI PspOMI PstI PvuII SacI SacII SanDI SbfI
SfcI SfiI SfoI SgrAI SmaI StuI StyI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AgeI AhdI AlfI AloI
AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BciVI BclI BfrBI BglII BmrI BmtI BplI
BpuEI BsaI BsaBI BsiWI BsmI BsrDI BsrGI BssHII
BstBI BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI
DrdI EarI EcoNI EcoRI EcoRV FalI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI NdeI
NheI NruI NsiI PacI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SapI ScaI SexAI SgfI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI