Restriction map for
pFN21AB5882
(Linear) (Six-base) MAPSORT of: KIBB5882.seq Check: 3525 from: 1 to: 1488
KIBB5882.orf
With 182 enzymes: SgfI *
July 3, 2012 16:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 742 780 1488
Size: 559 183 38 708
Fragments arranged by size:
708 559 183 38
AfeI AGC'GCT
Cuts at: 0 556 1327 1488
Size: 556 771 161
Fragments arranged by size:
771 556 161
AhdI GACnn_n'nnGTC
Cuts at: 0 377 1488
Size: 377 1111
AlwNI CAG_nnn'CTG
Cuts at: 0 672 1488
Size: 672 816
ApaI G_GGCC'C
Cuts at: 0 265 815 1421 1488
Size: 265 550 606 67
Fragments arranged by size:
606 550 265 67
ApoI r'AATT_y
Cuts at: 0 943 1488
Size: 943 545
AscI GG'CGCG_CC
Cuts at: 0 407 1488
Size: 407 1081
AvaI C'yCGr_G
Cuts at: 0 18 203 267 336 349 413 1329 1348
Size: 18 185 64 69 13 64 916 19
Cuts at: 1348 1488
Size: 140
Fragments arranged by size:
916 185 140 69 64 64 19 18
13
BanI G'GyrC_C
Cuts at: 0 226 245 621 1287 1488
Size: 226 19 376 666 201
Fragments arranged by size:
666 376 226 201 19
BanII G_rGCy'C
Cuts at: 0 265 307 815 1190 1355 1421 1488
Size: 265 42 508 375 165 66 67
Fragments arranged by size:
508 375 265 165 67 66 42
BbeI G_GCGC'C
Cuts at: 0 230 249 1488
Size: 230 19 1239
Fragments arranged by size:
1239 230 19
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1140 1174 1383 1417 1488
Size: 1140 34 209 34 71
Fragments arranged by size:
1140 209 71 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 142 442 647 1488
Size: 142 300 205 841
Fragments arranged by size:
841 300 205 142
BlpI GC'TnA_GC
Cuts at: 0 128 1177 1488
Size: 128 1049 311
Fragments arranged by size:
1049 311 128
Bme1580I G_kGCm'C
Cuts at: 0 265 815 1292 1421 1488
Size: 265 550 477 129 67
Fragments arranged by size:
550 477 265 129 67
BmgBI CAC'GTC
Cuts at: 0 318 1488
Size: 318 1170
BmrI ACTGGGnnnn_n'
Cuts at: 0 1017 1488
Size: 1017 471
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 523 595 1138 1483 1488
Size: 469 54 72 543 345 5
Fragments arranged by size:
543 469 345 72 54 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 1488
Size: 532 956
BsaAI yAC'GTr
Cuts at: 0 527 1488
Size: 527 961
BsaHI Gr'CG_yC
Cuts at: 0 227 246 398 419 953 971 1358 1488
Size: 227 19 152 21 534 18 387 130
Fragments arranged by size:
534 387 227 152 130 21 19 18
BsaWI w'CCGG_w
Cuts at: 0 966 1016 1488
Size: 966 50 472
Fragments arranged by size:
966 472 50
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 839 1462 1488
Size: 839 623 26
Fragments arranged by size:
839 623 26
BseYI C'CCAG_C
Cuts at: 0 298 1412 1488
Size: 298 1114 76
Fragments arranged by size:
1114 298 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1014 1488
Size: 1014 474
BsiEI CG_ry'CG
Cuts at: 0 63 168 681 1266 1488
Size: 63 105 513 585 222
Fragments arranged by size:
585 513 222 105 63
BsiHKAI G_wGCw'C
Cuts at: 0 307 1016 1223 1488
Size: 307 709 207 265
Fragments arranged by size:
709 307 265 207
BsmBI CGTCTCn'nnnn_
Cuts at: 0 358 1488
Size: 358 1130
Bsp1286I G_dGCh'C
Cuts at: 0 265 307 815 1016 1190 1223 1292 1355
Size: 265 42 508 201 174 33 69 63
Cuts at: 1355 1421 1488
Size: 66 67
Fragments arranged by size:
508 265 201 174 69 67 66 63
42 33
BspEI T'CCGG_A
Cuts at: 0 966 1488
Size: 966 522
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 382 710 1488
Size: 382 328 778
Fragments arranged by size:
778 382 328
BsrBI CCG'CTC
Cuts at: 0 170 393 1488
Size: 170 223 1095
Fragments arranged by size:
1095 223 170
BsrFI r'CCGG_y
Cuts at: 0 132 248 292 403 453 800 1488
Size: 132 116 44 111 50 347 688
Fragments arranged by size:
688 347 132 116 111 50 44
BssHII G'CGCG_C
Cuts at: 0 252 407 481 627 1488
Size: 252 155 74 146 861
Fragments arranged by size:
861 252 155 146 74
BstAPI GCAn_nnn'nTGC
Cuts at: 0 672 1488
Size: 672 816
BstEII G'GTnAC_C
Cuts at: 0 369 1488
Size: 369 1119
BstYI r'GATC_y
Cuts at: 0 873 1466 1488
Size: 873 593 22
Fragments arranged by size:
873 593 22
Bsu36I CC'TnA_GG
Cuts at: 0 1082 1488
Size: 1082 406
BtgI C'CryG_G
Cuts at: 0 139 193 591 713 1212 1488
Size: 139 54 398 122 499 276
Fragments arranged by size:
499 398 276 139 122 54
BtsI GCAGTG_nn'
Cuts at: 0 218 929 1488
Size: 218 711 559
Fragments arranged by size:
711 559 218
DraIII CAC_nnn'GTG
Cuts at: 0 714 1488
Size: 714 774
DrdI GACnn_nn'nnGTC
Cuts at: 0 1057 1488
Size: 1057 431
EaeI y'GGCC_r
Cuts at: 0 165 434 648 678 1263 1488
Size: 165 269 214 30 585 225
Fragments arranged by size:
585 269 225 214 165 30
EagI C'GGCC_G
Cuts at: 0 165 678 1263 1488
Size: 165 513 585 225
Fragments arranged by size:
585 513 225 165
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 169 170 337 483 628 812 1488
Size: 169 1 167 146 145 184 676
Fragments arranged by size:
676 184 169 167 146 145 1
EcoICRI GAG'CTC
Cuts at: 0 305 1488
Size: 305 1183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 523 559 595 742 780 1138 1483
Size: 469 54 36 36 147 38 358 345
Cuts at: 1483 1488
Size: 5
Fragments arranged by size:
469 358 345 147 54 38 36 36
5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1081 1488
Size: 1081 407
EcoO109I rG'GnC_Cy
Cuts at: 0 261 262 1417 1418 1488
Size: 261 1 1155 1 70
Fragments arranged by size:
1155 261 70 1 1
EcoRI G'AATT_C
Cuts at: 0 943 1488
Size: 943 545
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1179 1488
Size: 1179 309
FspI TGC'GCA
Cuts at: 0 1282 1488
Size: 1282 206
HaeII r_GCGC'y
Cuts at: 0 230 249 299 558 1329 1488
Size: 230 19 50 259 771 159
Fragments arranged by size:
771 259 230 159 50 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 363 395 1488
Size: 363 32 1093
Fragments arranged by size:
1093 363 32
HincII GTy'rAC
Cuts at: 0 1236 1488
Size: 1236 252
KasI G'GCGC_C
Cuts at: 0 226 245 1488
Size: 226 19 1243
Fragments arranged by size:
1243 226 19
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 1488
Size: 260 1228
MslI CAynn'nnrTG
Cuts at: 0 1309 1488
Size: 1309 179
MspA1I CmG'CkG
Cuts at: 0 80 141 195 593 669 1091 1214 1488
Size: 80 61 54 398 76 422 123 274
Fragments arranged by size:
422 398 274 123 80 76 61 54
NaeI GCC'GGC
Cuts at: 0 134 250 294 405 455 802 1488
Size: 134 116 44 111 50 347 686
Fragments arranged by size:
686 347 134 116 111 50 44
NarI GG'CG_CC
Cuts at: 0 227 246 1488
Size: 227 19 1242
Fragments arranged by size:
1242 227 19
NgoMIV G'CCGG_C
Cuts at: 0 132 248 292 403 453 800 1488
Size: 132 116 44 111 50 347 688
Fragments arranged by size:
688 347 132 116 111 50 44
NotI GC'GGCC_GC
Cuts at: 0 165 1263 1488
Size: 165 1098 225
Fragments arranged by size:
1098 225 165
NruI TCG'CGA
Cuts at: 0 476 854 1488
Size: 476 378 634
Fragments arranged by size:
634 476 378
NspI r_CATG'y
Cuts at: 0 963 1488
Size: 963 525
PasI CC'CwG_GG
Cuts at: 0 231 1488
Size: 231 1257
PflMI CCAn_nnn'nTGG
Cuts at: 0 1412 1488
Size: 1412 76
PspOMI G'GGCC_C
Cuts at: 0 261 811 1417 1488
Size: 261 550 606 71
Fragments arranged by size:
606 550 261 71
PstI C_TGCA'G
Cuts at: 0 707 1340 1488
Size: 707 633 148
Fragments arranged by size:
707 633 148
PvuII CAG'CTG
Cuts at: 0 669 1488
Size: 669 819
SacI G_AGCT'C
Cuts at: 0 307 1488
Size: 307 1181
SacII CC_GC'GG
Cuts at: 0 142 196 594 1215 1488
Size: 142 54 398 621 273
Fragments arranged by size:
621 398 273 142 54
SfcI C'TryA_G
Cuts at: 0 703 1336 1488
Size: 703 633 152
Fragments arranged by size:
703 633 152
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 442 1488
Size: 442 1046
SfoI GGC'GCC
Cuts at: 0 228 247 1488
Size: 228 19 1241
Fragments arranged by size:
1241 228 19
SgrAI Cr'CCGG_yG
Cuts at: 0 248 403 1488
Size: 248 155 1085
Fragments arranged by size:
1085 248 155
SmaI CCC'GGG
Cuts at: 0 205 338 415 1488
Size: 205 133 77 1073
Fragments arranged by size:
1073 205 133 77
SmlI C'TyrA_G
Cuts at: 0 547 1488
Size: 547 941
SrfI GCCC'GGGC
Cuts at: 0 338 1488
Size: 338 1150
StyI C'CwwG_G
Cuts at: 0 1406 1488
Size: 1406 82
TatI w'GTAC_w
Cuts at: 0 860 1488
Size: 860 628
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 429 1488
Size: 429 1059
XmaI C'CCGG_G
Cuts at: 0 203 336 413 1488
Size: 203 133 77 1075
Fragments arranged by size:
1075 203 133 77
Enzymes that do cut:
AcuI AfeI AhdI AlwNI ApaI ApoI AscI AvaI
BanI BanII BbeI BcgI BglI BlpI Bme1580I BmgBI
BmrI BpmI BpuEI BsaAI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrFI BssHII BstAPI BstEII BstYI Bsu36I BtgI BtsI
DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
MmeI MslI MspA1I NaeI NarI NgoMIV NotI NruI
NspI PasI PflMI PspOMI PstI PvuII SacI SacII
SfcI SfiI SfoI SgrAI SmaI SmlI SrfI StyI
TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI AlfI AloI ApaLI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BciVI BclI BfrBI BglII BmtI
BplI Bpu10I BsaI BsaBI BsaXI BsiWI BsmI BspHI
BsrDI BsrGI BssSI BstBI BstXI BstZ17I BtgZI ClaI
CspCI DraI EarI EcoRV FseI FspAI HindIII HpaI
KpnI MfeI MluI MscI NcoI NdeI NheI NsiI
PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI
SbfI ScaI SexAI SgfI SnaBI SpeI SphI SspI
StuI SwaI TaqII TstI Tth111I XbaI XhoI XmnI
ZraI