Restriction map for
pFN21AB5875
(Linear) (Six-base) MAPSORT of: KIBB5875.seq Check: 3538 from: 1 to: 1395
KIBB5875.orf
With 182 enzymes: SgfI *
July 3, 2012 16:10 ..
AclI AA'CG_TT
Cuts at: 0 668 1395
Size: 668 727
AfeI AGC'GCT
Cuts at: 0 353 1395
Size: 353 1042
AlwNI CAG_nnn'CTG
Cuts at: 0 36 579 1395
Size: 36 543 816
Fragments arranged by size:
816 543 36
ApaI G_GGCC'C
Cuts at: 0 1034 1395
Size: 1034 361
ApoI r'AATT_y
Cuts at: 0 1238 1395
Size: 1238 157
AvaI C'yCGr_G
Cuts at: 0 159 1312 1395
Size: 159 1153 83
Fragments arranged by size:
1153 159 83
BanI G'GyrC_C
Cuts at: 0 229 1395
Size: 229 1166
BanII G_rGCy'C
Cuts at: 0 797 1034 1395
Size: 797 237 361
Fragments arranged by size:
797 361 237
BbeI G_GCGC'C
Cuts at: 0 233 1395
Size: 233 1162
BbsI GAAGACnn'nnnn_
Cuts at: 0 1385 1395
Size: 1385 10
Bme1580I G_kGCm'C
Cuts at: 0 111 264 1034 1395
Size: 111 153 770 361
Fragments arranged by size:
770 361 153 111
BmrI ACTGGGnnnn_n'
Cuts at: 0 251 756 1395
Size: 251 505 639
Fragments arranged by size:
639 505 251
BmtI G_CTAG'C
Cuts at: 0 118 1395
Size: 118 1277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 303 427 947 1395
Size: 46 257 124 520 448
Fragments arranged by size:
520 448 257 124 46
Bpu10I CC'TnA_GC
Cuts at: 0 580 1306 1395
Size: 580 726 89
Fragments arranged by size:
726 580 89
BsaHI Gr'CG_yC
Cuts at: 0 230 1395
Size: 230 1165
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 301 331 1395
Size: 301 30 1064
Fragments arranged by size:
1064 301 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 204 241 283 1184 1333 1395
Size: 204 37 42 901 149 62
Fragments arranged by size:
901 204 149 62 42 37
BseYI C'CCAG_C
Cuts at: 0 354 1206 1395
Size: 354 852 189
Fragments arranged by size:
852 354 189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 822 1395
Size: 461 361 573
Fragments arranged by size:
573 461 361
BsiHKAI G_wGCw'C
Cuts at: 0 797 1395
Size: 797 598
BsmBI CGTCTCn'nnnn_
Cuts at: 0 548 1395
Size: 548 847
Bsp1286I G_dGCh'C
Cuts at: 0 111 264 797 1034 1395
Size: 111 153 533 237 361
Fragments arranged by size:
533 361 237 153 111
BsrBI CCG'CTC
Cuts at: 0 22 1395
Size: 22 1373
BsrFI r'CCGG_y
Cuts at: 0 241 283 1395
Size: 241 42 1112
Fragments arranged by size:
1112 241 42
BstBI TT'CG_AA
Cuts at: 0 912 1395
Size: 912 483
BstEII G'GTnAC_C
Cuts at: 0 39 1395
Size: 39 1356
BstXI CCAn_nnnn'nTGG
Cuts at: 0 672 1137 1395
Size: 672 465 258
Fragments arranged by size:
672 465 258
BstYI r'GATC_y
Cuts at: 0 176 1395
Size: 176 1219
BtgI C'CryG_G
Cuts at: 0 95 1395
Size: 95 1300
BtsI GCAGTG_nn'
Cuts at: 0 109 1395
Size: 109 1286
DraI TTT'AAA
Cuts at: 0 449 1395
Size: 449 946
EarI CTCTTCn'nnn_
Cuts at: 0 465 800 1052 1345 1395
Size: 465 335 252 293 50
Fragments arranged by size:
465 335 293 252 50
EcoICRI GAG'CTC
Cuts at: 0 795 1395
Size: 795 600
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 303 427 947 1395
Size: 46 257 124 520 448
Fragments arranged by size:
520 448 257 124 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1315 1395
Size: 1315 80
EcoO109I rG'GnC_Cy
Cuts at: 0 1030 1395
Size: 1030 365
EcoRV GAT'ATC
Cuts at: 0 660 1395
Size: 660 735
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 905 1395
Size: 905 490
HaeII r_GCGC'y
Cuts at: 0 233 355 1395
Size: 233 122 1040
Fragments arranged by size:
1040 233 122
HincII GTy'rAC
Cuts at: 0 860 1395
Size: 860 535
HpaI GTT'AAC
Cuts at: 0 860 1395
Size: 860 535
KasI G'GCGC_C
Cuts at: 0 229 1395
Size: 229 1166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1271 1395
Size: 1271 124
MspA1I CmG'CkG
Cuts at: 0 85 148 371 1395
Size: 85 63 223 1024
Fragments arranged by size:
1024 223 85 63
NaeI GCC'GGC
Cuts at: 0 243 285 1395
Size: 243 42 1110
Fragments arranged by size:
1110 243 42
NarI GG'CG_CC
Cuts at: 0 230 1395
Size: 230 1165
NcoI C'CATG_G
Cuts at: 0 95 1395
Size: 95 1300
NgoMIV G'CCGG_C
Cuts at: 0 241 283 1395
Size: 241 42 1112
Fragments arranged by size:
1112 241 42
NheI G'CTAG_C
Cuts at: 0 114 1395
Size: 114 1281
NspI r_CATG'y
Cuts at: 0 756 1395
Size: 756 639
PflMI CCAn_nnn'nTGG
Cuts at: 0 723 1395
Size: 723 672
PspOMI G'GGCC_C
Cuts at: 0 1030 1395
Size: 1030 365
PstI C_TGCA'G
Cuts at: 0 90 341 733 1395
Size: 90 251 392 662
Fragments arranged by size:
662 392 251 90
SacI G_AGCT'C
Cuts at: 0 797 1395
Size: 797 598
SapI GCTCTTCn'nnn_
Cuts at: 0 465 1395
Size: 465 930
SexAI A'CCwGG_T
Cuts at: 0 35 1395
Size: 35 1360
SfcI C'TryA_G
Cuts at: 0 86 337 729 1395
Size: 86 251 392 666
Fragments arranged by size:
666 392 251 86
SfoI GGC'GCC
Cuts at: 0 231 1395
Size: 231 1164
SmaI CCC'GGG
Cuts at: 0 161 1395
Size: 161 1234
SphI G_CATG'C
Cuts at: 0 756 1395
Size: 756 639
StuI AGG'CCT
Cuts at: 0 773 1395
Size: 773 622
StyI C'CwwG_G
Cuts at: 0 95 458 1026 1395
Size: 95 363 568 369
Fragments arranged by size:
568 369 363 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 805 1395
Size: 805 590
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 301 333 1250 1282 1395
Size: 301 32 917 32 113
Fragments arranged by size:
917 301 113 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 592 1102 1395
Size: 592 510 293
Fragments arranged by size:
592 510 293
XmaI C'CCGG_G
Cuts at: 0 159 1395
Size: 159 1236
XmnI GAAnn'nnTTC
Cuts at: 0 1188 1395
Size: 1188 207
Enzymes that do cut:
AclI AfeI AlwNI ApaI ApoI AvaI BanI BanII
BbeI BbsI Bme1580I BmrI BmtI BpmI Bpu10I BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrBI
BsrFI BstBI BstEII BstXI BstYI BtgI BtsI DraI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII
HincII HpaI KasI MmeI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PflMI PspOMI PstI SacI SapI
SexAI SfcI SfoI SmaI SphI StuI StyI TaqII
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BplI BpuEI BsaI BsaAI
BsaBI BsaWI BsiEI BsiWI BsmI BspEI BspHI BspMI
BsrDI BsrGI BssHII BssSI BstAPI BstZ17I Bsu36I BtgZI
ClaI CspCI DraIII DrdI EaeI EagI EciI EcoRI
FseI FspI FspAI Hin4I HindIII KpnI MfeI MluI
MscI MslI NdeI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI SwaI TatI Tth111I XbaI XhoI ZraI