Restriction map for
pFN21AB5872
(Linear) (Six-base) MAPSORT of: KIBB5872.seq Check: 8222 from: 1 to: 1152
KIBB5872.orf
With 182 enzymes: SgfI *
July 3, 2012 16:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 531 905 1152
Size: 531 374 247
Fragments arranged by size:
531 374 247
AccI GT'mk_AC
Cuts at: 0 362 1152
Size: 362 790
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 775 1152
Size: 340 435 377
Fragments arranged by size:
435 377 340
AfeI AGC'GCT
Cuts at: 0 888 1152
Size: 888 264
AflII C'TTAA_G
Cuts at: 0 877 1152
Size: 877 275
AlwNI CAG_nnn'CTG
Cuts at: 0 732 906 1152
Size: 732 174 246
Fragments arranged by size:
732 246 174
ApaI G_GGCC'C
Cuts at: 0 234 1152
Size: 234 918
ApaLI G'TGCA_C
Cuts at: 0 333 870 1152
Size: 333 537 282
Fragments arranged by size:
537 333 282
AscI GG'CGCG_CC
Cuts at: 0 53 1152
Size: 53 1099
AvaI C'yCGr_G
Cuts at: 0 130 204 213 253 402 1152
Size: 130 74 9 40 149 750
Fragments arranged by size:
750 149 130 74 40 9
BamHI G'GATC_C
Cuts at: 0 200 1152
Size: 200 952
BanI G'GyrC_C
Cuts at: 0 223 1152
Size: 223 929
BanII G_rGCy'C
Cuts at: 0 152 234 305 1152
Size: 152 82 71 847
Fragments arranged by size:
847 152 82 71
BbeI G_GCGC'C
Cuts at: 0 227 1152
Size: 227 925
BbsI GAAGACnn'nnnn_
Cuts at: 0 763 1152
Size: 763 389
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1128 1152
Size: 1128 24
BfrBI ATG'CAT
Cuts at: 0 387 1090 1152
Size: 387 703 62
Fragments arranged by size:
703 387 62
Bme1580I G_kGCm'C
Cuts at: 0 234 337 713 874 1152
Size: 234 103 376 161 278
Fragments arranged by size:
376 278 234 161 103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 1152
Size: 328 824
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 1152
Size: 136 1016
BsaI GGTCTCn'nnnn_
Cuts at: 0 349 1152
Size: 349 803
BsaHI Gr'CG_yC
Cuts at: 0 224 1152
Size: 224 928
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 1152
Size: 106 1046
BseYI C'CCAG_C
Cuts at: 0 422 681 1122 1152
Size: 422 259 441 30
Fragments arranged by size:
441 422 259 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 503 967 1152
Size: 424 79 464 185
Fragments arranged by size:
464 424 185 79
BsiEI CG_ry'CG
Cuts at: 0 188 747 1045 1152
Size: 188 559 298 107
Fragments arranged by size:
559 298 188 107
BsiHKAI G_wGCw'C
Cuts at: 0 152 337 874 1151 1152
Size: 152 185 537 277 1
Fragments arranged by size:
537 277 185 152 1
BsmI GAATG_Cn'
Cuts at: 0 1090 1152
Size: 1090 62
Bsp1286I G_dGCh'C
Cuts at: 0 152 234 305 337 713 874 1151 1152
Size: 152 82 71 32 376 161 277 1
Fragments arranged by size:
376 277 161 152 82 71 32 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 531 905 1152
Size: 531 374 247
Fragments arranged by size:
531 374 247
BsrDI GCAATG_nn'
Cuts at: 0 860 1152
Size: 860 292
BsrFI r'CCGG_y
Cuts at: 0 124 187 220 1152
Size: 124 63 33 932
Fragments arranged by size:
932 124 63 33
BsrGI T'GTAC_A
Cuts at: 0 347 833 1152
Size: 347 486 319
Fragments arranged by size:
486 347 319
BssHII G'CGCG_C
Cuts at: 0 53 1152
Size: 53 1099
BssSI C'ACGA_G
Cuts at: 0 163 1152
Size: 163 989
BstAPI GCAn_nnn'nTGC
Cuts at: 0 732 1152
Size: 732 420
BstEII G'GTnAC_C
Cuts at: 0 676 1152
Size: 676 476
BstYI r'GATC_y
Cuts at: 0 200 902 1152
Size: 200 702 250
Fragments arranged by size:
702 250 200
Bsu36I CC'TnA_GG
Cuts at: 0 597 717 1152
Size: 597 120 435
Fragments arranged by size:
597 435 120
BtgI C'CryG_G
Cuts at: 0 234 462 1152
Size: 234 228 690
Fragments arranged by size:
690 234 228
DraIII CAC_nnn'GTG
Cuts at: 0 627 1152
Size: 627 525
EaeI y'GGCC_r
Cuts at: 0 143 185 701 1152
Size: 143 42 516 451
Fragments arranged by size:
516 451 143 42
EagI C'GGCC_G
Cuts at: 0 185 1152
Size: 185 967
EarI CTCTTCn'nnn_
Cuts at: 0 286 932 1030 1152
Size: 286 646 98 122
Fragments arranged by size:
646 286 122 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 357 1152
Size: 357 795
EcoICRI GAG'CTC
Cuts at: 0 150 1152
Size: 150 1002
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 340 775 1152
Size: 328 12 435 377
Fragments arranged by size:
435 377 328 12
EcoO109I rG'GnC_Cy
Cuts at: 0 210 230 324 1152
Size: 210 20 94 828
Fragments arranged by size:
828 210 94 20
FspI TGC'GCA
Cuts at: 0 272 1152
Size: 272 880
HaeII r_GCGC'y
Cuts at: 0 122 227 890 1152
Size: 122 105 663 262
Fragments arranged by size:
663 262 122 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 139 171 842 874 1152
Size: 139 32 671 32 278
Fragments arranged by size:
671 278 139 32 32
KasI G'GCGC_C
Cuts at: 0 223 1152
Size: 223 929
MspA1I CmG'CkG
Cuts at: 0 9 236 341 438 981 1117 1152
Size: 9 227 105 97 543 136 35
Fragments arranged by size:
543 227 136 105 97 35 9
NaeI GCC'GGC
Cuts at: 0 189 222 1152
Size: 189 33 930
Fragments arranged by size:
930 189 33
NarI GG'CG_CC
Cuts at: 0 224 1152
Size: 224 928
NgoMIV G'CCGG_C
Cuts at: 0 187 220 1152
Size: 187 33 932
Fragments arranged by size:
932 187 33
NruI TCG'CGA
Cuts at: 0 933 1152
Size: 933 219
NsiI A_TGCA'T
Cuts at: 0 389 1092 1152
Size: 389 703 60
Fragments arranged by size:
703 389 60
NspI r_CATG'y
Cuts at: 0 348 990 1152
Size: 348 642 162
Fragments arranged by size:
642 348 162
PflMI CCAn_nnn'nTGG
Cuts at: 0 906 1152
Size: 906 246
PfoI T'CCnGG_A
Cuts at: 0 77 284 1152
Size: 77 207 868
Fragments arranged by size:
868 207 77
PpuMI rG'GwC_Cy
Cuts at: 0 210 1152
Size: 210 942
PspOMI G'GGCC_C
Cuts at: 0 230 1152
Size: 230 922
PstI C_TGCA'G
Cuts at: 0 563 737 1152
Size: 563 174 415
Fragments arranged by size:
563 415 174
PvuII CAG'CTG
Cuts at: 0 9 981 1152
Size: 9 972 171
Fragments arranged by size:
972 171 9
SacI G_AGCT'C
Cuts at: 0 152 1152
Size: 152 1000
SacII CC_GC'GG
Cuts at: 0 237 1152
Size: 237 915
SanDI GG'GwC_CC
Cuts at: 0 210 1152
Size: 210 942
SapI GCTCTTCn'nnn_
Cuts at: 0 932 1152
Size: 932 220
ScaI AGT'ACT
Cuts at: 0 952 1152
Size: 952 200
SexAI A'CCwGG_T
Cuts at: 0 672 1152
Size: 672 480
SfcI C'TryA_G
Cuts at: 0 559 733 1152
Size: 559 174 419
Fragments arranged by size:
559 419 174
SfoI GGC'GCC
Cuts at: 0 225 1152
Size: 225 927
SmaI CCC'GGG
Cuts at: 0 206 215 1152
Size: 206 9 937
Fragments arranged by size:
937 206 9
SmlI C'TyrA_G
Cuts at: 0 151 877 1152
Size: 151 726 275
Fragments arranged by size:
726 275 151
StuI AGG'CCT
Cuts at: 0 555 1152
Size: 555 597
StyI C'CwwG_G
Cuts at: 0 773 854 1152
Size: 773 81 298
Fragments arranged by size:
773 298 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 655 659 695 1152
Size: 655 4 36 457
Fragments arranged by size:
655 457 36 4
TatI w'GTAC_w
Cuts at: 0 347 833 950 1152
Size: 347 486 117 202
Fragments arranged by size:
486 347 202 117
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1048 1080 1152
Size: 1048 32 72
Fragments arranged by size:
1048 72 32
XmaI C'CCGG_G
Cuts at: 0 204 213 1152
Size: 204 9 939
Fragments arranged by size:
939 204 9
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AlwNI ApaI ApaLI
AscI AvaI BamHI BanI BanII BbeI BbsI BcgI
BfrBI Bme1580I BpmI BpuEI BsaI BsaHI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstYI Bsu36I BtgI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
FspI HaeII Hin4I KasI MspA1I NaeI NarI NgoMIV
NruI NsiI NspI PflMI PfoI PpuMI PspOMI PstI
PvuII SacI SacII SanDI SapI ScaI SexAI SfcI
SfoI SmaI SmlI StuI StyI TaqII TatI TstI
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflIII AgeI AhdI AleI AlfI
AloI ApoI AseI AsiSI AvrII BaeI BbvCI BciVI
BclI BglI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BsaAI BsaBI BsaWI BsaXI BsiWI BsmBI BspEI
BspHI BsrBI BstBI BstXI BstZ17I BtgZI BtsI ClaI
CspCI DraI DrdI EcoNI EcoRI EcoRV FalI FseI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI NcoI NdeI NheI NotI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SbfI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI
XhoI XmnI ZraI