(Linear) (Six-base) MAPSORT of: KIBB5860.seq  Check: 3671  from: 1  to: 1470



KIBB5860.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 16:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     109    1470 
   Size:        109    1361
 
AatII G_ACGT'C

Cuts at:      0     261    1470 
   Size:        261    1209
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427    1470 
   Size:        427    1043
 
AflII C'TTAA_G

Cuts at:      0     867    1470 
   Size:        867     603
 
AhdI GACnn_n'nnGTC

Cuts at:      0     861    1470 
   Size:        861     609
 
AlwNI CAG_nnn'CTG

Cuts at:      0     346    1470 
   Size:        346    1124
 
ApoI r'AATT_y

Cuts at:      0     528     931    1177    1470 
   Size:        528     403     246     293

  Fragments arranged by size: 

                 528     403     293     246
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     869     902    1470 
   Size:        869      33     568

  Fragments arranged by size: 

                 869     568      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     461     521    1401    1470 
   Size:        461      60     880      69

  Fragments arranged by size: 

                 880     461      69      60
 
BclI T'GATC_A

Cuts at:      0     198    1470 
   Size:        198    1272
 
BfrBI ATG'CAT

Cuts at:      0     494    1470 
   Size:        494     976
 
BglII A'GATC_T

Cuts at:      0     234    1470 
   Size:        234    1236
 
BmgBI CAC'GTC

Cuts at:      0    1028    1470 
   Size:       1028     442
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     262    1470 
   Size:        262    1208
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     912    1236    1470 
   Size:        912     324     234

  Fragments arranged by size: 

                 912     324     234
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     871    1470 
   Size:        871     599
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     636     996    1206    1470 
   Size:        636     360     210     264

  Fragments arranged by size: 

                 636     360     264     210
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1207    1470 
   Size:       1207     263
 
BsaHI Gr'CG_yC

Cuts at:      0     258    1470 
   Size:        258    1212
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     586    1470 
   Size:        586     884
 
BseYI C'CCAG_C

Cuts at:      0    1408    1470 
   Size:       1408      62
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     437    1470 
   Size:        437    1033
 
BsmI GAATG_Cn'

Cuts at:      0     928    1470 
   Size:        928     542
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1328    1470 
   Size:       1328     142
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     109    1470 
   Size:        109    1361
 
BssSI C'ACGA_G

Cuts at:      0    1331    1470 
   Size:       1331     139
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     121    1470 
   Size:        121    1349
 
BstEII G'GTnAC_C

Cuts at:      0     891    1470 
   Size:        891     579
 
BstYI r'GATC_y

Cuts at:      0      53     234    1470 
   Size:         53     181    1236

  Fragments arranged by size: 

                1236     181      53
 
BtsI GCAGTG_nn'

Cuts at:      0     329     469     919    1037    1470 
   Size:        329     140     450     118     433

  Fragments arranged by size: 

                 450     433     329     140     118
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     871    1470 
   Size:        427     444     599

  Fragments arranged by size: 

                 599     444     427
 
EcoRI G'AATT_C

Cuts at:      0    1177    1470 
   Size:       1177     293
 
EcoRV GAT'ATC

Cuts at:      0     462    1035    1470 
   Size:        462     573     435

  Fragments arranged by size: 

                 573     462     435
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     449    1470 
   Size:        449    1021
 
FspI TGC'GCA

Cuts at:      0     125    1470 
   Size:        125    1345
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     345     377     532     564     718     750     847     879 
   Size:        345      32     155      32     154      32      97      32

Cuts at:    879    1350    1382    1470 
   Size:        471      32      88

  Fragments arranged by size: 

                 471     345     155     154      97      88      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    1010    1470 
   Size:       1010     460
 
HpaI GTT'AAC

Cuts at:      0    1010    1470 
   Size:       1010     460
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     732    1470 
   Size:        732     738
 
MslI CAynn'nnrTG

Cuts at:      0     181    1470 
   Size:        181    1289
 
MspA1I CmG'CkG

Cuts at:      0    1446    1470 
   Size:       1446      24
 
NdeI CA'TA_TG

Cuts at:      0     215    1470 
   Size:        215    1255
 
NsiI A_TGCA'T

Cuts at:      0     496    1470 
   Size:        496     974
 
NspI r_CATG'y

Cuts at:      0     115    1470 
   Size:        115    1355
 
PfoI T'CCnGG_A

Cuts at:      0      60    1470 
   Size:         60    1410
 
PvuII CAG'CTG

Cuts at:      0    1446    1470 
   Size:       1446      24
 
SfcI C'TryA_G

Cuts at:      0     338     449     701    1470 
   Size:        338     111     252     769

  Fragments arranged by size: 

                 769     338     252     111
 
SmlI C'TyrA_G

Cuts at:      0     615     867     975    1221    1470 
   Size:        615     252     108     246     249

  Fragments arranged by size: 

                 615     252     249     246     108
 
SphI G_CATG'C

Cuts at:      0     115    1470 
   Size:        115    1355
 
StyI C'CwwG_G

Cuts at:      0     561     595    1470 
   Size:        561      34     875

  Fragments arranged by size: 

                 875     561      34
 
TatI w'GTAC_w

Cuts at:      0     173     916    1470 
   Size:        173     743     554

  Fragments arranged by size: 

                 743     554     173
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     795     827    1470 
   Size:        795      32     643

  Fragments arranged by size: 

                 795     643      32
 
XbaI T'CTAG_A

Cuts at:      0     502    1470 
   Size:        502     968
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1224    1470 
   Size:       1224     246
 
XmnI GAAnn'nnTTC

Cuts at:      0    1095    1470 
   Size:       1095     375
 
ZraI GAC'GTC

Cuts at:      0     259    1470 
   Size:        259    1211
 

 Enzymes that do cut:

     AarI    AatII     AcuI    AflII     AhdI    AlwNI     ApoI     BaeI
     BbsI     BclI    BfrBI    BglII    BmgBI     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaHI    BseRI    BseYI     BsgI     BsmI    BsmBI
    BspMI    BssSI   BstAPI   BstEII    BstYI     BtsI  Eco57MI    EcoRI
    EcoRV     FalI     FspI    Hin4I   HincII     HpaI     MmeI     MslI
   MspA1I     NdeI     NsiI     NspI     PfoI    PvuII     SfcI     SmlI
     SphI     StyI     TatI     TstI     XbaI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI   AflIII     AgeI     AleI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BglI
     BlpI Bme1580I     BmtI   Bpu10I    BsaAI    BsaBI    BsaWI    BsaXI
    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BstBI    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I     FseI    FspAI    HaeII
  HindIII     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
    TaqII  Tth111I     XhoI     XmaI