Restriction map for
pFN21AB5846
(Linear) (Six-base) MAPSORT of: KIBB5846.seq Check: 2430 from: 1 to: 1455
KIBB5846.orf
With 182 enzymes: SgfI *
July 3, 2012 16:08 ..
Acc65I G'GTAC_C
Cuts at: 0 883 1455
Size: 883 572
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 1377 1455
Size: 253 1124 78
Fragments arranged by size:
1124 253 78
AflII C'TTAA_G
Cuts at: 0 127 1455
Size: 127 1328
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 518 550 1455
Size: 518 32 905
Fragments arranged by size:
905 518 32
AlwNI CAG_nnn'CTG
Cuts at: 0 231 264 291 1455
Size: 231 33 27 1164
Fragments arranged by size:
1164 231 33 27
ApoI r'AATT_y
Cuts at: 0 82 590 853 1175 1259 1343 1427 1455
Size: 82 508 263 322 84 84 84 28
Fragments arranged by size:
508 322 263 84 84 84 82 28
AseI AT'TA_AT
Cuts at: 0 1450 1455
Size: 1450 5
BanI G'GyrC_C
Cuts at: 0 883 1455
Size: 883 572
BanII G_rGCy'C
Cuts at: 0 140 218 901 985 1153 1321 1405 1455
Size: 140 78 683 84 168 168 84 50
Fragments arranged by size:
683 168 168 140 84 84 78 50
BciVI GTATCCnnnnn_n'
Cuts at: 0 634 1455
Size: 634 821
BglII A'GATC_T
Cuts at: 0 1155 1455
Size: 1155 300
BmrI ACTGGGnnnn_n'
Cuts at: 0 1193 1455
Size: 1193 262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 391 965 1121 1373 1455
Size: 23 368 574 156 252 82
Fragments arranged by size:
574 368 252 156 82 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 1455
Size: 653 802
BsaI GGTCTCn'nnnn_
Cuts at: 0 493 1455
Size: 493 962
BsaBI GATnn'nnATC
Cuts at: 0 645 1455
Size: 645 810
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 518 548 883 913 1455
Size: 518 30 335 30 542
Fragments arranged by size:
542 518 335 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 367 843 1455
Size: 367 476 612
Fragments arranged by size:
612 476 367
BseYI C'CCAG_C
Cuts at: 0 530 1455
Size: 530 925
BsiHKAI G_wGCw'C
Cuts at: 0 140 218 901 1153 1321 1405 1455
Size: 140 78 683 252 168 84 50
Fragments arranged by size:
683 252 168 140 84 78 50
BsmI GAATG_Cn'
Cuts at: 0 1222 1375 1455
Size: 1222 153 80
Fragments arranged by size:
1222 153 80
Bsp1286I G_dGCh'C
Cuts at: 0 140 218 901 985 1153 1321 1405 1455
Size: 140 78 683 84 168 168 84 50
Fragments arranged by size:
683 168 168 140 84 84 78 50
BstXI CCAn_nnnn'nTGG
Cuts at: 0 427 1455
Size: 427 1028
BstYI r'GATC_y
Cuts at: 0 242 571 1155 1455
Size: 242 329 584 300
Fragments arranged by size:
584 329 300 242
BtgI C'CryG_G
Cuts at: 0 90 1455
Size: 90 1365
BtsI GCAGTG_nn'
Cuts at: 0 365 1380 1455
Size: 365 1015 75
Fragments arranged by size:
1015 365 75
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1194 1229 1455
Size: 1194 35 226
Fragments arranged by size:
1194 226 35
EarI CTCTTCn'nnn_
Cuts at: 0 1380 1455
Size: 1380 75
EcoICRI GAG'CTC
Cuts at: 0 138 216 899 1151 1319 1403 1455
Size: 138 78 683 252 168 84 52
Fragments arranged by size:
683 252 168 138 84 78 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 253 391 965 1121 1373 1377 1455
Size: 23 230 138 574 156 252 4 78
Fragments arranged by size:
574 252 230 156 138 78 23 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 130 1455
Size: 130 1325
EcoRI G'AATT_C
Cuts at: 0 853 1175 1259 1343 1427 1455
Size: 853 322 84 84 84 28
Fragments arranged by size:
853 322 84 84 84 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 822 854 883 915 1140 1172 1308 1340
Size: 822 32 29 32 225 32 136 32
Cuts at: 1340 1455
Size: 115
Fragments arranged by size:
822 225 136 115 32 32 32 32
29
HincII GTy'rAC
Cuts at: 0 1417 1455
Size: 1417 38
HindIII A'AGCT_T
Cuts at: 0 1052 1137 1455
Size: 1052 85 318
Fragments arranged by size:
1052 318 85
KpnI G_GTAC'C
Cuts at: 0 887 1455
Size: 887 568
MslI CAynn'nnrTG
Cuts at: 0 425 962 1455
Size: 425 537 493
Fragments arranged by size:
537 493 425
NcoI C'CATG_G
Cuts at: 0 90 1455
Size: 90 1365
NruI TCG'CGA
Cuts at: 0 191 1455
Size: 191 1264
NspI r_CATG'y
Cuts at: 0 1003 1455
Size: 1003 452
PflMI CCAn_nnn'nTGG
Cuts at: 0 346 1455
Size: 346 1109
PfoI T'CCnGG_A
Cuts at: 0 180 209 462 1455
Size: 180 29 253 993
Fragments arranged by size:
993 253 180 29
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 518 550 1455
Size: 518 32 905
Fragments arranged by size:
905 518 32
PshAI GACnn'nnGTC
Cuts at: 0 741 1455
Size: 741 714
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 395 427 1455
Size: 395 32 1028
Fragments arranged by size:
1028 395 32
PstI C_TGCA'G
Cuts at: 0 27 145 690 1455
Size: 27 118 545 765
Fragments arranged by size:
765 545 118 27
SacI G_AGCT'C
Cuts at: 0 140 218 901 1153 1321 1405 1455
Size: 140 78 683 252 168 84 50
Fragments arranged by size:
683 252 168 140 84 78 50
SapI GCTCTTCn'nnn_
Cuts at: 0 1380 1455
Size: 1380 75
ScaI AGT'ACT
Cuts at: 0 404 1455
Size: 404 1051
SfcI C'TryA_G
Cuts at: 0 5 23 141 686 1455
Size: 5 18 118 545 769
Fragments arranged by size:
769 545 118 18 5
SmlI C'TyrA_G
Cuts at: 0 127 668 1455
Size: 127 541 787
Fragments arranged by size:
787 541 127
SspI AAT'ATT
Cuts at: 0 440 1455
Size: 440 1015
StuI AGG'CCT
Cuts at: 0 319 1455
Size: 319 1136
StyI C'CwwG_G
Cuts at: 0 90 251 503 1455
Size: 90 161 252 952
Fragments arranged by size:
952 252 161 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 793 1455
Size: 793 662
TatI w'GTAC_w
Cuts at: 0 402 1455
Size: 402 1053
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 178 582 1455
Size: 178 404 873
Fragments arranged by size:
873 404 178
XmnI GAAnn'nnTTC
Cuts at: 0 53 260 1054 1306 1455
Size: 53 207 794 252 149
Fragments arranged by size:
794 252 207 149 53
Enzymes that do cut:
Acc65I AcuI AflII AloI AlwNI ApoI AseI BanI
BanII BciVI BglII BmrI BpmI BpuEI BsaI BsaBI
BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BstXI BstYI
BtgI BtsI CspCI EarI EcoICRI Eco57MI EcoNI EcoRI
Hin4I HincII HindIII KpnI MslI NcoI NruI NspI
PflMI PfoI PpiI PshAI PsrI PstI SacI SapI
ScaI SfcI SmlI SspI StuI StyI TaqII TatI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflIII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BclI BfrBI
BglI BlpI Bme1580I BmgBI BmtI BplI Bpu10I BsaAI
BsaHI BsaWI BsgI BsiEI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI DraIII
DrdI EaeI EagI EciI EcoO109I EcoRV FalI FseI
FspI FspAI HaeII HpaI KasI MfeI MluI MmeI
MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NsiI PacI PasI PciI PmeI PmlI PpuMI PsiI
PspOMI PspXI PvuI PvuII RsrII SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TstI Tth111I XbaI XhoI
XmaI ZraI