(Linear) (Six-base) MAPSORT of: KIBB5838.seq  Check: 109  from: 1  to: 1443



KIBB5838.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 15:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1195    1443 
   Size:       1195     248
 
AccI GT'mk_AC

Cuts at:      0     793    1136    1443 
   Size:        793     343     307

  Fragments arranged by size: 

                 793     343     307
 
AclI AA'CG_TT

Cuts at:      0     636    1443 
   Size:        636     807
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1442    1443 
   Size:       1442       1
 
AleI CACnn'nnGTG

Cuts at:      0     619    1443 
   Size:        619     824
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     455    1443 
   Size:        455     988
 
ApoI r'AATT_y

Cuts at:      0    1295    1443 
   Size:       1295     148
 
AvrII C'CTAG_G

Cuts at:      0     975    1443 
   Size:        975     468
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1355    1443 
   Size:       1355      88
 
BfrBI ATG'CAT

Cuts at:      0     432    1168    1443 
   Size:        432     736     275

  Fragments arranged by size: 

                 736     432     275
 
BglII A'GATC_T

Cuts at:      0    1241    1443 
   Size:       1241     202
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     174    1443 
   Size:        174    1269
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262    1105    1218    1443 
   Size:        262     843     113     225

  Fragments arranged by size: 

                 843     262     225     113
 
Bpu10I CC'TnA_GC

Cuts at:      0      44    1443 
   Size:         44    1399
 
BsaWI w'CCGG_w

Cuts at:      0     831    1250    1443 
   Size:        831     419     193

  Fragments arranged by size: 

                 831     419     193
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1384    1443 
   Size:       1384      59
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     958    1443 
   Size:        145     813     485

  Fragments arranged by size: 

                 813     485     145
 
BsiHKAI G_wGCw'C

Cuts at:      0      50      64    1443 
   Size:         50      14    1379

  Fragments arranged by size: 

                1379      50      14
 
BsmI GAATG_Cn'

Cuts at:      0     514    1443 
   Size:        514     929
 
Bsp1286I G_dGCh'C

Cuts at:      0      50      64    1443 
   Size:         50      14    1379

  Fragments arranged by size: 

                1379      50      14
 
BspEI T'CCGG_A

Cuts at:      0    1250    1443 
   Size:       1250     193
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1195    1443 
   Size:       1195     248
 
BsrDI GCAATG_nn'

Cuts at:      0     554     851    1443 
   Size:        554     297     592

  Fragments arranged by size: 

                 592     554     297
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      54    1443 
   Size:         54    1389
 
BstBI TT'CG_AA

Cuts at:      0    1299    1443 
   Size:       1299     144
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     450    1443 
   Size:        450     993
 
BstYI r'GATC_y

Cuts at:      0    1241    1443 
   Size:       1241     202
 
Bsu36I CC'TnA_GG

Cuts at:      0    1116    1443 
   Size:       1116     327
 
EaeI y'GGCC_r

Cuts at:      0    1173    1443 
   Size:       1173     270
 
EarI CTCTTCn'nnn_

Cuts at:      0      87     179    1150    1418    1443 
   Size:         87      92     971     268      25

  Fragments arranged by size: 

                 971     268      92      87      25
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262    1105    1218    1442    1443 
   Size:        262     843     113     224       1

  Fragments arranged by size: 

                 843     262     224     113       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     575    1443 
   Size:        575     868
 
EcoO109I rG'GnC_Cy

Cuts at:      0     972    1443 
   Size:        972     471
 
EcoRI G'AATT_C

Cuts at:      0    1295    1443 
   Size:       1295     148
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     762    1263    1443 
   Size:        762     501     180

  Fragments arranged by size: 

                 762     501     180
 
HincII GTy'rAC

Cuts at:      0     785    1443 
   Size:        785     658
 
MfeI C'AATT_G

Cuts at:      0     203     326     920    1443 
   Size:        203     123     594     523

  Fragments arranged by size: 

                 594     523     203     123
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     288     974    1443 
   Size:        288     686     469

  Fragments arranged by size: 

                 686     469     288
 
MslI CAynn'nnrTG

Cuts at:      0     437     448     619    1443 
   Size:        437      11     171     824

  Fragments arranged by size: 

                 824     437     171      11
 
NsiI A_TGCA'T

Cuts at:      0     434    1170    1443 
   Size:        434     736     273

  Fragments arranged by size: 

                 736     434     273
 
PfoI T'CCnGG_A

Cuts at:      0    1082    1443 
   Size:       1082     361
 
PpuMI rG'GwC_Cy

Cuts at:      0     972    1443 
   Size:        972     471
 
PstI C_TGCA'G

Cuts at:      0     257    1443 
   Size:        257    1186
 
SapI GCTCTTCn'nnn_

Cuts at:      0     179    1418    1443 
   Size:        179    1239      25

  Fragments arranged by size: 

                1239     179      25
 
SfcI C'TryA_G

Cuts at:      0     253     509     794    1443 
   Size:        253     256     285     649

  Fragments arranged by size: 

                 649     285     256     253
 
StyI C'CwwG_G

Cuts at:      0     284     975    1443 
   Size:        284     691     468

  Fragments arranged by size: 

                 691     468     284
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     302     334    1443 
   Size:        302      32    1109

  Fragments arranged by size: 

                1109     302      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     789    1443 
   Size:        789     654
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI     AleI     AlfI     ApoI    AvrII
     BbsI    BfrBI    BglII     BmrI     BpmI   Bpu10I    BsaWI    BseRI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI    BsrDI   BstAPI
    BstBI    BstXI    BstYI   Bsu36I     EaeI     EarI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI   HincII     MfeI     MmeI     MslI     NsiI
     PfoI    PpuMI     PstI     SapI     SfcI     StyI     TstI  Tth111I

 Enzymes that do not cut: 

    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI     AloI
    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI     BaeI
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI
     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseYI    BsiEI    BsiWI    BsmBI
    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstEII  BstZ17I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I  HindIII     HpaI     KasI     KpnI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI