Restriction map for
pFN21AB5805
(Linear) (Six-base) MAPSORT of: KIBB5805.seq Check: 7851 from: 1 to: 1413
KIBB5805.orf
With 182 enzymes: SgfI *
July 3, 2012 16:07 ..
AatII G_ACGT'C
Cuts at: 0 949 1413
Size: 949 464
AflIII A'CryG_T
Cuts at: 0 1151 1413
Size: 1151 262
AgeI A'CCGG_T
Cuts at: 0 1328 1413
Size: 1328 85
ApaI G_GGCC'C
Cuts at: 0 543 1039 1413
Size: 543 496 374
Fragments arranged by size:
543 496 374
AscI GG'CGCG_CC
Cuts at: 0 956 1413
Size: 956 457
AvaI C'yCGr_G
Cuts at: 0 144 543 898 1038 1315 1335 1413
Size: 144 399 355 140 277 20 78
Fragments arranged by size:
399 355 277 144 140 78 20
BanI G'GyrC_C
Cuts at: 0 30 286 509 831 876 1092 1413
Size: 30 256 223 322 45 216 321
Fragments arranged by size:
322 321 256 223 216 45 30
BanII G_rGCy'C
Cuts at: 0 127 151 193 543 760 892 1039 1355
Size: 127 24 42 350 217 132 147 316
Cuts at: 1355 1413
Size: 58
Fragments arranged by size:
350 316 217 147 132 127 58 42
24
BbeI G_GCGC'C
Cuts at: 0 34 290 513 835 880 1413
Size: 34 256 223 322 45 533
Fragments arranged by size:
533 322 256 223 45 34
BglI GCCn_nnn'nGGC
Cuts at: 0 163 1056 1078 1413
Size: 163 893 22 335
Fragments arranged by size:
893 335 163 22
BlpI GC'TnA_GC
Cuts at: 0 886 1413
Size: 886 527
Bme1580I G_kGCm'C
Cuts at: 0 61 543 631 1039 1095 1413
Size: 61 482 88 408 56 318
Fragments arranged by size:
482 408 318 88 61 56
BmgBI CAC'GTC
Cuts at: 0 1010 1154 1413
Size: 1010 144 259
Fragments arranged by size:
1010 259 144
BmtI G_CTAG'C
Cuts at: 0 451 1413
Size: 451 962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 1413
Size: 808 605
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 499 1003 1413
Size: 370 129 504 410
Fragments arranged by size:
504 410 370 129
BsaI GGTCTCn'nnnn_
Cuts at: 0 486 628 1222 1413
Size: 486 142 594 191
Fragments arranged by size:
594 486 191 142
BsaAI yAC'GTr
Cuts at: 0 669 1413
Size: 669 744
BsaHI Gr'CG_yC
Cuts at: 0 31 118 212 287 371 510 832 877
Size: 31 87 94 75 84 139 322 45
Cuts at: 877 928 946 1397 1413
Size: 51 18 451 16
Fragments arranged by size:
451 322 139 94 87 84 75 51
45 31 18 16
BsaWI w'CCGG_w
Cuts at: 0 1328 1413
Size: 1328 85
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 826 1413
Size: 115 711 587
Fragments arranged by size:
711 587 115
BseYI C'CCAG_C
Cuts at: 0 1131 1413
Size: 1131 282
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 1246 1362 1413
Size: 649 597 116 51
Fragments arranged by size:
649 597 116 51
BsiEI CG_ry'CG
Cuts at: 0 209 701 1070 1241 1413
Size: 209 492 369 171 172
Fragments arranged by size:
492 369 209 172 171
BsiHKAI G_wGCw'C
Cuts at: 0 407 697 1413
Size: 407 290 716
Fragments arranged by size:
716 407 290
BsiWI C'GTAC_G
Cuts at: 0 305 1413
Size: 305 1108
BsmBI CGTCTCn'nnnn_
Cuts at: 0 953 1413
Size: 953 460
Bsp1286I G_dGCh'C
Cuts at: 0 61 127 151 193 407 543 631 697
Size: 61 66 24 42 214 136 88 66
Cuts at: 697 760 892 1039 1095 1355 1413
Size: 63 132 147 56 260 58
Fragments arranged by size:
260 214 147 136 132 88 66 66
63 61 58 56 42 24
BsrBI CCG'CTC
Cuts at: 0 822 1314 1413
Size: 822 492 99
Fragments arranged by size:
822 492 99
BsrFI r'CCGG_y
Cuts at: 0 178 229 274 283 506 865 1077 1328
Size: 178 51 45 9 223 359 212 251
Cuts at: 1328 1413
Size: 85
Fragments arranged by size:
359 251 223 212 178 85 51 45
9
BssHII G'CGCG_C
Cuts at: 0 109 502 956 1304 1413
Size: 109 393 454 348 109
Fragments arranged by size:
454 393 348 109 109
BssSI C'ACGA_G
Cuts at: 0 54 690 1413
Size: 54 636 723
Fragments arranged by size:
723 636 54
BtgI C'CryG_G
Cuts at: 0 374 912 1064 1241 1382 1413
Size: 374 538 152 177 141 31
Fragments arranged by size:
538 374 177 152 141 31
EaeI y'GGCC_r
Cuts at: 0 173 206 377 698 918 995 1067 1238
Size: 173 33 171 321 220 77 72 171
Cuts at: 1238 1280 1413
Size: 42 133
Fragments arranged by size:
321 220 173 171 171 133 77 72
42 33
EagI C'GGCC_G
Cuts at: 0 206 698 1067 1238 1413
Size: 206 492 369 171 175
Fragments arranged by size:
492 369 206 175 171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 1413
Size: 808 605
EcoNI CCTnn'n_nnAGG
Cuts at: 0 25 475 517 1413
Size: 25 450 42 896
Fragments arranged by size:
896 450 42 25
EcoO109I rG'GnC_Cy
Cuts at: 0 540 738 825 1257 1413
Size: 540 198 87 432 156
Fragments arranged by size:
540 432 198 156 87
HaeII r_GCGC'y
Cuts at: 0 34 49 290 513 835 880 1204 1413
Size: 34 15 241 223 322 45 324 209
Fragments arranged by size:
324 322 241 223 209 45 34 15
HindIII A'AGCT_T
Cuts at: 0 718 1413
Size: 718 695
KasI G'GCGC_C
Cuts at: 0 30 286 509 831 876 1413
Size: 30 256 223 322 45 537
Fragments arranged by size:
537 322 256 223 45 30
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 992 1413
Size: 686 306 421
Fragments arranged by size:
686 421 306
MslI CAynn'nnrTG
Cuts at: 0 277 463 1413
Size: 277 186 950
Fragments arranged by size:
950 277 186
MspA1I CmG'CkG
Cuts at: 0 195 882 914 1066 1128 1243 1413
Size: 195 687 32 152 62 115 170
Fragments arranged by size:
687 195 170 152 115 62 32
NaeI GCC'GGC
Cuts at: 0 231 508 1079 1413
Size: 231 277 571 334
Fragments arranged by size:
571 334 277 231
NarI GG'CG_CC
Cuts at: 0 31 287 510 832 877 1413
Size: 31 256 223 322 45 536
Fragments arranged by size:
536 322 256 223 45 31
NgoMIV G'CCGG_C
Cuts at: 0 229 506 1077 1413
Size: 229 277 571 336
Fragments arranged by size:
571 336 277 229
NheI G'CTAG_C
Cuts at: 0 447 1413
Size: 447 966
NotI GC'GGCC_GC
Cuts at: 0 1067 1238 1413
Size: 1067 171 175
Fragments arranged by size:
1067 175 171
PasI CC'CwG_GG
Cuts at: 0 933 1413
Size: 933 480
PflMI CCAn_nnn'nTGG
Cuts at: 0 986 1413
Size: 986 427
PmlI CAC'GTG
Cuts at: 0 669 1413
Size: 669 744
PpuMI rG'GwC_Cy
Cuts at: 0 738 1413
Size: 738 675
PshAI GACnn'nnGTC
Cuts at: 0 605 1413
Size: 605 808
PspOMI G'GGCC_C
Cuts at: 0 539 1035 1413
Size: 539 496 378
Fragments arranged by size:
539 496 378
PspXI vC'TCGA_Gb
Cuts at: 0 1315 1413
Size: 1315 98
PstI C_TGCA'G
Cuts at: 0 200 1208 1413
Size: 200 1008 205
Fragments arranged by size:
1008 205 200
SacII CC_GC'GG
Cuts at: 0 915 1067 1244 1413
Size: 915 152 177 169
Fragments arranged by size:
915 177 169 152
SanDI GG'GwC_CC
Cuts at: 0 738 1413
Size: 738 675
ScaI AGT'ACT
Cuts at: 0 202 1413
Size: 202 1211
SexAI A'CCwGG_T
Cuts at: 0 487 1413
Size: 487 926
SfcI C'TryA_G
Cuts at: 0 196 1204 1413
Size: 196 1008 209
Fragments arranged by size:
1008 209 196
SfoI GGC'GCC
Cuts at: 0 32 288 511 833 878 1413
Size: 32 256 223 322 45 535
Fragments arranged by size:
535 322 256 223 45 32
SgrAI Cr'CCGG_yG
Cuts at: 0 506 1413
Size: 506 907
SmaI CCC'GGG
Cuts at: 0 146 1040 1337 1413
Size: 146 894 297 76
Fragments arranged by size:
894 297 146 76
SmlI C'TyrA_G
Cuts at: 0 385 514 1018 1315 1413
Size: 385 129 504 297 98
Fragments arranged by size:
504 385 297 129 98
SrfI GCCC'GGGC
Cuts at: 0 1040 1413
Size: 1040 373
StuI AGG'CCT
Cuts at: 0 391 1413
Size: 391 1022
StyI C'CwwG_G
Cuts at: 0 428 1413
Size: 428 985
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 490 1413
Size: 490 923
TatI w'GTAC_w
Cuts at: 0 200 1413
Size: 200 1213
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 676 708 730 762 1315 1347 1413
Size: 676 32 22 32 553 32 66
Fragments arranged by size:
676 553 66 32 32 32 22
Tth111I GACn'n_nGTC
Cuts at: 0 852 1413
Size: 852 561
XhoI C'TCGA_G
Cuts at: 0 1315 1413
Size: 1315 98
XmaI C'CCGG_G
Cuts at: 0 144 1038 1335 1413
Size: 144 894 297 78
Fragments arranged by size:
894 297 144 78
ZraI GAC'GTC
Cuts at: 0 947 1413
Size: 947 466
Enzymes that do cut:
AatII AflIII AgeI ApaI AscI AvaI BanI BanII
BbeI BglI BlpI Bme1580I BmgBI BmtI BpmI BpuEI
BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BsrBI BsrFI BssHII BssSI
BtgI EaeI EagI Eco57MI EcoNI EcoO109I HaeII HindIII
KasI MmeI MslI MspA1I NaeI NarI NgoMIV NheI
NotI PasI PflMI PmlI PpuMI PshAI PspOMI PspXI
PstI SacII SanDI ScaI SexAI SfcI SfoI SgrAI
SmaI SmlI SrfI StuI StyI TaqII TatI TstI
Tth111I XhoI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BmrI BplI Bpu10I BsaBI BsaXI BsmI
BspEI BspHI BspMI BsrDI BsrGI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EarI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HpaI KpnI
MfeI MluI MscI NcoI NdeI NruI NsiI NspI
PacI PciI PfoI PmeI PpiI PsiI PsrI PvuI
PvuII RsrII SacI SalI SapI SbfI SfiI SgfI
SnaBI SpeI SphI SspI SwaI XbaI XcmI XmnI