Restriction map for
pFN21AB5793
(Linear) (Six-base) MAPSORT of: KIBB5793.seq Check: 6802 from: 1 to: 2493
KIBB5793.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AccI GT'mk_AC
Cuts at: 0 88 1238 2493
Size: 88 1150 1255
Fragments arranged by size:
1255 1150 88
Acc65I G'GTAC_C
Cuts at: 0 2141 2493
Size: 2141 352
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 851 1703 1901 2101 2493
Size: 576 275 852 198 200 392
Fragments arranged by size:
852 576 392 275 200 198
AflIII A'CryG_T
Cuts at: 0 1679 2493
Size: 1679 814
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2408 2493
Size: 2408 85
AlwNI CAG_nnn'CTG
Cuts at: 0 78 1413 1726 1879 1926 2493
Size: 78 1335 313 153 47 567
Fragments arranged by size:
1335 567 313 153 78 47
ApoI r'AATT_y
Cuts at: 0 505 615 675 729 2150 2250 2262 2493
Size: 505 110 60 54 1421 100 12 231
Fragments arranged by size:
1421 505 231 110 100 60 54 12
BamHI G'GATC_C
Cuts at: 0 977 2493
Size: 977 1516
BanI G'GyrC_C
Cuts at: 0 2141 2493
Size: 2141 352
BanII G_rGCy'C
Cuts at: 0 849 2493
Size: 849 1644
BbvCI CC'TCA_GC
Cuts at: 0 94 2493
Size: 94 2399
BciVI GTATCCnnnnn_n'
Cuts at: 0 345 957 2493
Size: 345 612 1536
Fragments arranged by size:
1536 612 345
BclI T'GATC_A
Cuts at: 0 1283 2493
Size: 1283 1210
BfrBI ATG'CAT
Cuts at: 0 1089 2493
Size: 1089 1404
BglI GCCn_nnn'nGGC
Cuts at: 0 286 2493
Size: 286 2207
BglII A'GATC_T
Cuts at: 0 474 630 2493
Size: 474 156 1863
Fragments arranged by size:
1863 474 156
BmrI ACTGGGnnnn_n'
Cuts at: 0 371 1121 2493
Size: 371 750 1372
Fragments arranged by size:
1372 750 371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 1124 1610 1748 2029 2116 2493
Size: 587 537 486 138 281 87 377
Fragments arranged by size:
587 537 486 377 281 138 87
Bpu10I CC'TnA_GC
Cuts at: 0 94 2493
Size: 94 2399
BsaI GGTCTCn'nnnn_
Cuts at: 0 1797 2493
Size: 1797 696
BsaHI Gr'CG_yC
Cuts at: 0 225 1235 2493
Size: 225 1010 1258
Fragments arranged by size:
1258 1010 225
BsaWI w'CCGG_w
Cuts at: 0 2144 2238 2493
Size: 2144 94 255
Fragments arranged by size:
2144 255 94
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1207 1237 1387 1417 2422 2452 2493
Size: 1207 30 150 30 1005 30 41
Fragments arranged by size:
1207 1005 150 41 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 52 55 265 2493
Size: 49 3 3 210 2228
Fragments arranged by size:
2228 210 49 3 3
BseYI C'CCAG_C
Cuts at: 0 170 1019 2493
Size: 170 849 1474
Fragments arranged by size:
1474 849 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1392 2493
Size: 1392 1101
BsiEI CG_ry'CG
Cuts at: 0 188 219 261 2493
Size: 188 31 42 2232
Fragments arranged by size:
2232 188 42 31
BsiHKAI G_wGCw'C
Cuts at: 0 1256 1616 2327 2493
Size: 1256 360 711 166
Fragments arranged by size:
1256 711 360 166
BsmI GAATG_Cn'
Cuts at: 0 1452 2493
Size: 1452 1041
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1195 2493
Size: 1195 1298
Bsp1286I G_dGCh'C
Cuts at: 0 849 1256 1616 2327 2493
Size: 849 407 360 711 166
Fragments arranged by size:
849 711 407 360 166
BspEI T'CCGG_A
Cuts at: 0 2238 2493
Size: 2238 255
BspHI T'CATG_A
Cuts at: 0 2463 2493
Size: 2463 30
BsrBI CCG'CTC
Cuts at: 0 6 2493
Size: 6 2487
BsrDI GCAATG_nn'
Cuts at: 0 1463 2493
Size: 1463 1030
BsrFI r'CCGG_y
Cuts at: 0 104 2493
Size: 104 2389
BsrGI T'GTAC_A
Cuts at: 0 1514 2493
Size: 1514 979
BssHII G'CGCG_C
Cuts at: 0 233 2493
Size: 233 2260
BstAPI GCAn_nnn'nTGC
Cuts at: 0 78 2493
Size: 78 2415
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1167 1860 2064 2409 2493
Size: 1167 693 204 345 84
Fragments arranged by size:
1167 693 345 204 84
BstYI r'GATC_y
Cuts at: 0 474 630 977 2493
Size: 474 156 347 1516
Fragments arranged by size:
1516 474 347 156
Bsu36I CC'TnA_GG
Cuts at: 0 1318 2493
Size: 1318 1175
BtgI C'CryG_G
Cuts at: 0 45 210 1031 1230 2493
Size: 45 165 821 199 1263
Fragments arranged by size:
1263 821 199 165 45
ClaI AT'CG_AT
Cuts at: 0 2370 2493
Size: 2370 123
DraI TTT'AAA
Cuts at: 0 1812 2493
Size: 1812 681
EaeI y'GGCC_r
Cuts at: 0 185 216 258 287 773 1604 2282 2493
Size: 185 31 42 29 486 831 678 211
Fragments arranged by size:
831 678 486 211 185 42 31 29
EagI C'GGCC_G
Cuts at: 0 185 216 258 2493
Size: 185 31 42 2235
Fragments arranged by size:
2235 185 42 31
EarI CTCTTCn'nnn_
Cuts at: 0 1832 2109 2493
Size: 1832 277 384
Fragments arranged by size:
1832 384 277
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1211 2493
Size: 1211 1282
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 587 851 1124 1610 1703 1748 1901
Size: 576 11 264 273 486 93 45 153
Cuts at: 1901 2029 2101 2116 2493
Size: 128 72 15 377
Fragments arranged by size:
576 486 377 273 264 153 128 93
72 45 15 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 97 2493
Size: 97 2396
EcoO109I rG'GnC_Cy
Cuts at: 0 1561 1692 2481 2493
Size: 1561 131 789 12
Fragments arranged by size:
1561 789 131 12
EcoRI G'AATT_C
Cuts at: 0 675 2250 2493
Size: 675 1575 243
Fragments arranged by size:
1575 675 243
EcoRV GAT'ATC
Cuts at: 0 1818 2493
Size: 1818 675
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 856 1837 2493
Size: 856 981 656
Fragments arranged by size:
981 856 656
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 572 604 1136 1168 1477 1509
Size: 16 32 524 32 532 32 309 32
Cuts at: 1509 2422 2454 2493
Size: 913 32 39
Fragments arranged by size:
913 532 524 309 39 32 32 32
32 32 16
HincII GTy'rAC
Cuts at: 0 89 551 2493
Size: 89 462 1942
Fragments arranged by size:
1942 462 89
HindIII A'AGCT_T
Cuts at: 0 882 2493
Size: 882 1611
HpaI GTT'AAC
Cuts at: 0 551 2493
Size: 551 1942
KpnI G_GTAC'C
Cuts at: 0 2145 2493
Size: 2145 348
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 2493
Size: 330 2163
MscI TGG'CCA
Cuts at: 0 289 775 1606 2284 2493
Size: 289 486 831 678 209
Fragments arranged by size:
831 678 486 289 209
MslI CAynn'nnrTG
Cuts at: 0 163 305 655 1094 1385 1495 1600 1947
Size: 163 142 350 439 291 110 105 347
Cuts at: 1947 2493
Size: 546
Fragments arranged by size:
546 439 350 347 291 163 142 110
105
MspA1I CmG'CkG
Cuts at: 0 75 130 138 212 1232 2493
Size: 75 55 8 74 1020 1261
Fragments arranged by size:
1261 1020 75 74 55 8
NcoI C'CATG_G
Cuts at: 0 45 2493
Size: 45 2448
NdeI CA'TA_TG
Cuts at: 0 1855 2493
Size: 1855 638
NotI GC'GGCC_GC
Cuts at: 0 185 2493
Size: 185 2308
NsiI A_TGCA'T
Cuts at: 0 1091 2493
Size: 1091 1402
NspI r_CATG'y
Cuts at: 0 310 1093 2395 2493
Size: 310 783 1302 98
Fragments arranged by size:
1302 783 310 98
PasI CC'CwG_GG
Cuts at: 0 11 2493
Size: 11 2482
PflMI CCAn_nnn'nTGG
Cuts at: 0 1787 2094 2493
Size: 1787 307 399
Fragments arranged by size:
1787 399 307
PfoI T'CCnGG_A
Cuts at: 0 266 1540 1580 2493
Size: 266 1274 40 913
Fragments arranged by size:
1274 913 266 40
PpuMI rG'GwC_Cy
Cuts at: 0 1561 1692 2481 2493
Size: 1561 131 789 12
Fragments arranged by size:
1561 789 131 12
PshAI GACnn'nnGTC
Cuts at: 0 1658 2493
Size: 1658 835
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2171 2203 2493
Size: 2171 32 290
Fragments arranged by size:
2171 290 32
PstI C_TGCA'G
Cuts at: 0 74 2387 2493
Size: 74 2313 106
Fragments arranged by size:
2313 106 74
PvuII CAG'CTG
Cuts at: 0 75 2493
Size: 75 2418
SacII CC_GC'GG
Cuts at: 0 213 1233 2493
Size: 213 1020 1260
Fragments arranged by size:
1260 1020 213
SalI G'TCGA_C
Cuts at: 0 87 2493
Size: 87 2406
SanDI GG'GwC_CC
Cuts at: 0 1692 2493
Size: 1692 801
SfcI C'TryA_G
Cuts at: 0 70 1349 1395 2383 2493
Size: 70 1279 46 988 110
Fragments arranged by size:
1279 988 110 70 46
SphI G_CATG'C
Cuts at: 0 2395 2493
Size: 2395 98
StyI C'CwwG_G
Cuts at: 0 45 840 1296 2493
Size: 45 795 456 1197
Fragments arranged by size:
1197 795 456 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 708 1187 2493
Size: 708 479 1306
Fragments arranged by size:
1306 708 479
TatI w'GTAC_w
Cuts at: 0 1514 2493
Size: 1514 979
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 238 270 530 562 2493
Size: 238 32 260 32 1931
Fragments arranged by size:
1931 260 238 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 274 2493
Size: 274 2219
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1497 2493
Size: 1497 996
XmnI GAAnn'nnTTC
Cuts at: 0 1922 2493
Size: 1922 571
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlfI AlwNI ApoI BamHI
BanI BanII BbvCI BciVI BclI BfrBI BglI BglII
BmrI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstXI
BstYI Bsu36I BtgI ClaI DraI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I
HincII HindIII HpaI KpnI MmeI MscI MslI MspA1I
NcoI NdeI NotI NsiI NspI PasI PflMI PfoI
PpuMI PshAI PsrI PstI PvuII SacII SalI SanDI
SfcI SphI StyI TaqII TatI TstI Tth111I XcmI
XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BbsI BcgI BlpI Bme1580I BmgBI BmtI
BplI BpuEI BsaAI BsaBI BsiWI BspMI BssSI BstBI
BstEII BstZ17I BtgZI BtsI CspCI DraIII DrdI EcoICRI
FseI FspI FspAI HaeII KasI MfeI MluI NaeI
NarI NgoMIV NheI NruI PacI PciI PmeI PmlI
PpiI PsiI PspOMI PspXI PvuI RsrII SacI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StuI SwaI XbaI
XhoI XmaI ZraI