Restriction map for
pFN21AB5790
(Linear) (Six-base) MAPSORT of: KIBB5790.seq Check: 1253 from: 1 to: 2331
KIBB5790.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2080 2331
Size: 2080 251
AccI GT'mk_AC
Cuts at: 0 1917 2331
Size: 1917 414
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 848 1441 1718 1982 2331
Size: 467 381 593 277 264 349
Fragments arranged by size:
593 467 381 349 277 264
AfeI AGC'GCT
Cuts at: 0 2191 2331
Size: 2191 140
AflII C'TTAA_G
Cuts at: 0 1774 2331
Size: 1774 557
AflIII A'CryG_T
Cuts at: 0 1145 2331
Size: 1145 1186
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 231 263 2331
Size: 231 32 2068
Fragments arranged by size:
2068 231 32
AlwNI CAG_nnn'CTG
Cuts at: 0 805 2331
Size: 805 1526
ApaI G_GGCC'C
Cuts at: 0 105 2331
Size: 105 2226
ApoI r'AATT_y
Cuts at: 0 336 420 541 654 682 1156 1894 1955
Size: 336 84 121 113 28 474 738 61
Cuts at: 1955 2331
Size: 376
Fragments arranged by size:
738 474 376 336 121 113 84 61
28
AvrII C'CTAG_G
Cuts at: 0 382 2331
Size: 382 1949
BamHI G'GATC_C
Cuts at: 0 38 2331
Size: 38 2293
BanI G'GyrC_C
Cuts at: 0 94 1053 1415 2331
Size: 94 959 362 916
Fragments arranged by size:
959 916 362 94
BanII G_rGCy'C
Cuts at: 0 105 2331
Size: 105 2226
BbsI GAAGACnn'nnnn_
Cuts at: 0 1724 2331
Size: 1724 607
BbvCI CC'TCA_GC
Cuts at: 0 1233 1656 2331
Size: 1233 423 675
Fragments arranged by size:
1233 675 423
BciVI GTATCCnnnnn_n'
Cuts at: 0 266 729 2331
Size: 266 463 1602
Fragments arranged by size:
1602 463 266
BclI T'GATC_A
Cuts at: 0 2184 2331
Size: 2184 147
BglII A'GATC_T
Cuts at: 0 601 2331
Size: 601 1730
BlpI GC'TnA_GC
Cuts at: 0 524 848 2331
Size: 524 324 1483
Fragments arranged by size:
1483 524 324
Bme1580I G_kGCm'C
Cuts at: 0 105 1058 1418 2331
Size: 105 953 360 913
Fragments arranged by size:
953 913 360 105
BmgBI CAC'GTC
Cuts at: 0 2136 2331
Size: 2136 195
BmrI ACTGGGnnnn_n'
Cuts at: 0 1694 2331
Size: 1694 637
BmtI G_CTAG'C
Cuts at: 0 1664 2331
Size: 1664 667
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 732 848 1426 1539 2331
Size: 151 581 116 578 113 792
Fragments arranged by size:
792 581 578 151 116 113
Bpu10I CC'TnA_GC
Cuts at: 0 1233 1656 2331
Size: 1233 423 675
Fragments arranged by size:
1233 675 423
BsaI GGTCTCn'nnnn_
Cuts at: 0 2166 2331
Size: 2166 165
BsaBI GATnn'nnATC
Cuts at: 0 2183 2331
Size: 2183 148
BsaWI w'CCGG_w
Cuts at: 0 81 2331
Size: 81 2250
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1025 1055 2331
Size: 1025 30 1276
Fragments arranged by size:
1276 1025 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1295 2331
Size: 1295 1036
BseYI C'CCAG_C
Cuts at: 0 494 2331
Size: 494 1837
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2181 2331
Size: 2181 150
BsiEI CG_ry'CG
Cuts at: 0 126 2331
Size: 126 2205
BsiHKAI G_wGCw'C
Cuts at: 0 203 525 1027 2331
Size: 203 322 502 1304
Fragments arranged by size:
1304 502 322 203
BsmI GAATG_Cn'
Cuts at: 0 1016 2331
Size: 1016 1315
Bsp1286I G_dGCh'C
Cuts at: 0 105 203 525 1027 1058 1418 2331
Size: 105 98 322 502 31 360 913
Fragments arranged by size:
913 502 360 322 105 98 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2080 2331
Size: 2080 251
BsrBI CCG'CTC
Cuts at: 0 44 2331
Size: 44 2287
BsrDI GCAATG_nn'
Cuts at: 0 1072 1244 1390 2331
Size: 1072 172 146 941
Fragments arranged by size:
1072 941 172 146
BsrFI r'CCGG_y
Cuts at: 0 26 2331
Size: 26 2305
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1053 2167 2331
Size: 1053 1114 164
Fragments arranged by size:
1114 1053 164
BstBI TT'CG_AA
Cuts at: 0 333 2331
Size: 333 1998
BstYI r'GATC_y
Cuts at: 0 38 601 2102 2331
Size: 38 563 1501 229
Fragments arranged by size:
1501 563 229 38
BtgI C'CryG_G
Cuts at: 0 74 2331
Size: 74 2257
BtsI GCAGTG_nn'
Cuts at: 0 1665 1728 2084 2331
Size: 1665 63 356 247
Fragments arranged by size:
1665 356 247 63
DrdI GACnn_nn'nnGTC
Cuts at: 0 986 2331
Size: 986 1345
EaeI y'GGCC_r
Cuts at: 0 966 2331
Size: 966 1365
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 60 2331
Size: 60 2271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 467 732 848 1426 1441 1539 1718
Size: 151 316 265 116 578 15 98 179
Cuts at: 1718 1982 2331
Size: 264 349
Fragments arranged by size:
578 349 316 265 264 179 151 116
98 15
EcoRI G'AATT_C
Cuts at: 0 420 1156 2331
Size: 420 736 1175
Fragments arranged by size:
1175 736 420
HaeII r_GCGC'y
Cuts at: 0 2193 2331
Size: 2193 138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1212 1244 2331
Size: 1212 32 1087
Fragments arranged by size:
1212 1087 32
HindIII A'AGCT_T
Cuts at: 0 328 479 1400 2331
Size: 328 151 921 931
Fragments arranged by size:
931 921 328 151
MscI TGG'CCA
Cuts at: 0 968 2331
Size: 968 1363
MslI CAynn'nnrTG
Cuts at: 0 350 1098 2331
Size: 350 748 1233
Fragments arranged by size:
1233 748 350
MspA1I CmG'CkG
Cuts at: 0 1998 2331
Size: 1998 333
NcoI C'CATG_G
Cuts at: 0 74 2331
Size: 74 2257
NdeI CA'TA_TG
Cuts at: 0 537 2331
Size: 537 1794
NheI G'CTAG_C
Cuts at: 0 1660 2331
Size: 1660 671
NspI r_CATG'y
Cuts at: 0 1149 1519 1916 2331
Size: 1149 370 397 415
Fragments arranged by size:
1149 415 397 370
PciI A'CATG_T
Cuts at: 0 1145 2331
Size: 1145 1186
PfoI T'CCnGG_A
Cuts at: 0 46 2331
Size: 46 2285
PspOMI G'GGCC_C
Cuts at: 0 101 2331
Size: 101 2230
PstI C_TGCA'G
Cuts at: 0 567 2091 2331
Size: 567 1524 240
Fragments arranged by size:
1524 567 240
PvuII CAG'CTG
Cuts at: 0 1998 2331
Size: 1998 333
SexAI A'CCwGG_T
Cuts at: 0 1173 2331
Size: 1173 1158
SfcI C'TryA_G
Cuts at: 0 210 563 726 2087 2331
Size: 210 353 163 1361 244
Fragments arranged by size:
1361 353 244 210 163
SmlI C'TyrA_G
Cuts at: 0 1774 2331
Size: 1774 557
StyI C'CwwG_G
Cuts at: 0 74 382 2331
Size: 74 308 1949
Fragments arranged by size:
1949 308 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2252 2331
Size: 2252 79
TatI w'GTAC_w
Cuts at: 0 157 1710 1804 2331
Size: 157 1553 94 527
Fragments arranged by size:
1553 527 157 94
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 236 268 2331
Size: 236 32 2063
Fragments arranged by size:
2063 236 32
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AloI AlwNI
ApaI ApoI AvrII BamHI BanI BanII BbsI BbvCI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BsaI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI
BsrFI BstAPI BstBI BstYI BtgI BtsI DrdI EaeI
EciI Eco57MI EcoRI HaeII Hin4I HindIII MscI MslI
MspA1I NcoI NdeI NheI NspI PciI PfoI PspOMI
PstI PvuII SexAI SfcI SmlI StyI TaqII TatI
TstI
Enzymes that do not cut:
AatII Acc65I AclI AgeI AhdI AleI AlfI ApaLI
AscI AseI AsiSI AvaI BaeI BbeI BcgI BfrBI
BglI BplI BpuEI BsaAI BsaHI BsiWI BsmBI BspEI
BspHI BsrGI BssHII BssSI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII EagI EarI EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HincII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NgoMIV NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI