Restriction map for
pFN21AB5788
(Linear) (Six-base) MAPSORT of: KIBB5788.seq Check: 614 from: 1 to: 1056
KIBB5788.orf
With 182 enzymes: SgfI *
July 3, 2012 16:06 ..
AccI GT'mk_AC
Cuts at: 0 659 992 1056
Size: 659 333 64
Fragments arranged by size:
659 333 64
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 604 643 1056
Size: 155 449 39 413
Fragments arranged by size:
449 413 155 39
AflIII A'CryG_T
Cuts at: 0 938 1056
Size: 938 118
AhdI GACnn_n'nnGTC
Cuts at: 0 1026 1056
Size: 1026 30
AvaI C'yCGr_G
Cuts at: 0 485 1056
Size: 485 571
BanII G_rGCy'C
Cuts at: 0 11 494 1056
Size: 11 483 562
Fragments arranged by size:
562 483 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 176 527 1056
Size: 176 351 529
Fragments arranged by size:
529 351 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1012 1046 1056
Size: 1012 34 10
Fragments arranged by size:
1012 34 10
BglI GCCn_nnn'nGGC
Cuts at: 0 256 314 1039 1056
Size: 256 58 725 17
Fragments arranged by size:
725 256 58 17
BlpI GC'TnA_GC
Cuts at: 0 5 1056
Size: 5 1051
Bme1580I G_kGCm'C
Cuts at: 0 572 1056
Size: 572 484
BmgBI CAC'GTC
Cuts at: 0 819 941 1056
Size: 819 122 115
Fragments arranged by size:
819 122 115
BmtI G_CTAG'C
Cuts at: 0 1018 1056
Size: 1018 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 1056
Size: 287 769
Bpu10I CC'TnA_GC
Cuts at: 0 676 1056
Size: 676 380
BsaAI yAC'GTr
Cuts at: 0 189 1056
Size: 189 867
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 895 925 1056
Size: 895 30 131
Fragments arranged by size:
895 131 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 957 1056
Size: 957 99
BseYI C'CCAG_C
Cuts at: 0 43 160 373 1001 1056
Size: 43 117 213 628 55
Fragments arranged by size:
628 213 117 55 43
BsiWI C'GTAC_G
Cuts at: 0 50 1056
Size: 50 1006
BsmBI CGTCTCn'nnnn_
Cuts at: 0 947 1056
Size: 947 109
Bsp1286I G_dGCh'C
Cuts at: 0 11 494 572 1056
Size: 11 483 78 484
Fragments arranged by size:
484 483 78 11
BsrBI CCG'CTC
Cuts at: 0 358 452 1056
Size: 358 94 604
Fragments arranged by size:
604 358 94
BsrDI GCAATG_nn'
Cuts at: 0 966 1056
Size: 966 90
BsrFI r'CCGG_y
Cuts at: 0 973 1056
Size: 973 83
BsrGI T'GTAC_A
Cuts at: 0 911 1056
Size: 911 145
BstBI TT'CG_AA
Cuts at: 0 23 1056
Size: 23 1033
BstEII G'GTnAC_C
Cuts at: 0 684 1056
Size: 684 372
BstXI CCAn_nnnn'nTGG
Cuts at: 0 501 823 1056
Size: 501 322 233
Fragments arranged by size:
501 322 233
BtgI C'CryG_G
Cuts at: 0 286 894 946 1056
Size: 286 608 52 110
Fragments arranged by size:
608 286 110 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 251 1056
Size: 251 805
BtsI GCAGTG_nn'
Cuts at: 0 512 1056
Size: 512 544
EaeI y'GGCC_r
Cuts at: 0 735 1040 1056
Size: 735 305 16
Fragments arranged by size:
735 305 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 528 842 916 1056
Size: 528 314 74 140
Fragments arranged by size:
528 314 140 74
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 287 604 643 1056
Size: 155 132 317 39 413
Fragments arranged by size:
413 317 155 132 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 679 827 1056
Size: 679 148 229
Fragments arranged by size:
679 229 148
EcoO109I rG'GnC_Cy
Cuts at: 0 797 1056
Size: 797 259
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 783 879 1056
Size: 783 96 177
Fragments arranged by size:
783 177 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 21 53 183 215 1012 1044 1056
Size: 21 32 130 32 797 32 12
Fragments arranged by size:
797 130 32 32 32 21 12
HincII GTy'rAC
Cuts at: 0 672 1056
Size: 672 384
HindIII A'AGCT_T
Cuts at: 0 760 1056
Size: 760 296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 1056
Size: 496 560
MscI TGG'CCA
Cuts at: 0 737 1042 1056
Size: 737 305 14
Fragments arranged by size:
737 305 14
MslI CAynn'nnrTG
Cuts at: 0 985 1056
Size: 985 71
MspA1I CmG'CkG
Cuts at: 0 288 410 1056
Size: 288 122 646
Fragments arranged by size:
646 288 122
NheI G'CTAG_C
Cuts at: 0 1014 1056
Size: 1014 42
PflMI CCAn_nnn'nTGG
Cuts at: 0 733 1056
Size: 733 323
PmlI CAC'GTG
Cuts at: 0 189 1056
Size: 189 867
PpuMI rG'GwC_Cy
Cuts at: 0 797 1056
Size: 797 259
PstI C_TGCA'G
Cuts at: 0 557 1056
Size: 557 499
SacII CC_GC'GG
Cuts at: 0 289 1056
Size: 289 767
SanDI GG'GwC_CC
Cuts at: 0 797 1056
Size: 797 259
SfcI C'TryA_G
Cuts at: 0 414 553 1056
Size: 414 139 503
Fragments arranged by size:
503 414 139
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 314 1056
Size: 314 742
SmaI CCC'GGG
Cuts at: 0 487 1056
Size: 487 569
StyI C'CwwG_G
Cuts at: 0 251 1056
Size: 251 805
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 387 495 537 829 1056
Size: 387 108 42 292 227
Fragments arranged by size:
387 292 227 108 42
TatI w'GTAC_w
Cuts at: 0 911 1056
Size: 911 145
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 321 353 1056
Size: 321 32 703
Fragments arranged by size:
703 321 32
Tth111I GACn'n_nGTC
Cuts at: 0 778 1056
Size: 778 278
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 790 1056
Size: 790 266
XmaI C'CCGG_G
Cuts at: 0 485 1056
Size: 485 571
Enzymes that do cut:
AccI AcuI AflIII AhdI AvaI BanII BbsI BcgI
BglI BlpI Bme1580I BmgBI BmtI BpmI Bpu10I BsaAI
BsaXI BseRI BseYI BsiWI BsmBI Bsp1286I BsrBI BsrDI
BsrFI BsrGI BstBI BstEII BstXI BtgI BtgZI BtsI
EaeI EciI Eco57MI EcoNI EcoO109I FalI Hin4I HincII
HindIII MmeI MscI MslI MspA1I NheI PflMI PmlI
PpuMI PstI SacII SanDI SfcI SfiI SmaI StyI
TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BciVI
BclI BfrBI BglII BmrI BplI BpuEI BsaI BsaBI
BsaHI BsaWI BsgI BsiEI BsiHKAI BsmI BspEI BspHI
BspMI BssHII BssSI BstAPI BstYI BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoRI
EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI XbaI XhoI XmnI ZraI