Restriction map for
pFN21AB5785
(Linear) (Six-base) MAPSORT of: KIBB5785.seq Check: 643 from: 1 to: 2403
KIBB5785.orf
With 182 enzymes: SgfI *
March 8, 2011 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 288 2403
Size: 288 2115
AatII G_ACGT'C
Cuts at: 0 2071 2403
Size: 2071 332
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 1275 2403
Size: 644 631 1128
Fragments arranged by size:
1128 644 631
AfeI AGC'GCT
Cuts at: 0 820 2403
Size: 820 1583
AflIII A'CryG_T
Cuts at: 0 542 2126 2403
Size: 542 1584 277
Fragments arranged by size:
1584 542 277
AleI CACnn'nnGTG
Cuts at: 0 692 2403
Size: 692 1711
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1930 1940 2403
Size: 1930 10 463
Fragments arranged by size:
1930 463 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1435 1467 2403
Size: 1435 32 936
Fragments arranged by size:
1435 936 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1008 2316 2403
Size: 1008 1308 87
Fragments arranged by size:
1308 1008 87
ApaLI G'TGCA_C
Cuts at: 0 1898 2403
Size: 1898 505
ApoI r'AATT_y
Cuts at: 0 934 1220 2403
Size: 934 286 1183
Fragments arranged by size:
1183 934 286
AvaI C'yCGr_G
Cuts at: 0 1049 1210 2403
Size: 1049 161 1193
Fragments arranged by size:
1193 1049 161
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 32 2403
Size: 32 2371
BamHI G'GATC_C
Cuts at: 0 1205 2403
Size: 1205 1198
BanI G'GyrC_C
Cuts at: 0 214 1478 2403
Size: 214 1264 925
Fragments arranged by size:
1264 925 214
BanII G_rGCy'C
Cuts at: 0 50 775 1022 1367 1562 1597 2403
Size: 50 725 247 345 195 35 806
Fragments arranged by size:
806 725 345 247 195 50 35
BbeI G_GCGC'C
Cuts at: 0 1482 2403
Size: 1482 921
BbsI GAAGACnn'nnnn_
Cuts at: 0 357 2403
Size: 357 2046
BbvCI CC'TCA_GC
Cuts at: 0 391 2403
Size: 391 2012
BglI GCCn_nnn'nGGC
Cuts at: 0 148 322 2120 2403
Size: 148 174 1798 283
Fragments arranged by size:
1798 283 174 148
Bme1580I G_kGCm'C
Cuts at: 0 639 1304 1730 1902 2403
Size: 639 665 426 172 501
Fragments arranged by size:
665 639 501 426 172
BmgBI CAC'GTC
Cuts at: 0 513 567 2403
Size: 513 54 1836
Fragments arranged by size:
1836 513 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 525 1398 2403
Size: 525 873 1005
Fragments arranged by size:
1005 873 525
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 780 2403
Size: 780 1623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 482 613 2022 2235 2403
Size: 244 238 131 1409 213 168
Fragments arranged by size:
1409 244 238 213 168 131
Bpu10I CC'TnA_GC
Cuts at: 0 391 2403
Size: 391 2012
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 789 2403
Size: 789 1614
BsaI GGTCTCn'nnnn_
Cuts at: 0 1517 2403
Size: 1517 886
BsaAI yAC'GTr
Cuts at: 0 1086 2403
Size: 1086 1317
BsaBI GATnn'nnATC
Cuts at: 0 955 2403
Size: 955 1448
BsaHI Gr'CG_yC
Cuts at: 0 1479 2068 2403
Size: 1479 589 335
Fragments arranged by size:
1479 589 335
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 26 1384 1414 1435 1465 2403
Size: 26 1358 30 21 30 938
Fragments arranged by size:
1358 938 30 30 26 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 310 2314 2403
Size: 310 2004 89
Fragments arranged by size:
2004 310 89
BseYI C'CCAG_C
Cuts at: 0 1927 2403
Size: 1927 476
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2218 2403
Size: 2218 185
BsiEI CG_ry'CG
Cuts at: 0 959 2403
Size: 959 1444
BsiHKAI G_wGCw'C
Cuts at: 0 1022 1367 1868 1902 2403
Size: 1022 345 501 34 501
Fragments arranged by size:
1022 501 501 345 34
BsmI GAATG_Cn'
Cuts at: 0 1888 2403
Size: 1888 515
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2059 2403
Size: 2059 344
Bsp1286I G_dGCh'C
Cuts at: 0 50 639 775 1022 1304 1367 1562 1597
Size: 50 589 136 247 282 63 195 35
Cuts at: 1597 1730 1868 1902 2403
Size: 133 138 34 501
Fragments arranged by size:
589 501 282 247 195 138 136 133
63 50 35 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 288 1958 2403
Size: 288 1670 445
Fragments arranged by size:
1670 445 288
BsrBI CCG'CTC
Cuts at: 0 2173 2403
Size: 2173 230
BsrDI GCAATG_nn'
Cuts at: 0 1454 2403
Size: 1454 949
BsrFI r'CCGG_y
Cuts at: 0 485 1475 2403
Size: 485 990 928
Fragments arranged by size:
990 928 485
BsrGI T'GTAC_A
Cuts at: 0 545 2403
Size: 545 1858
BstBI TT'CG_AA
Cuts at: 0 2262 2403
Size: 2262 141
BstYI r'GATC_y
Cuts at: 0 257 1205 1533 2403
Size: 257 948 328 870
Fragments arranged by size:
948 870 328 257
Bsu36I CC'TnA_GG
Cuts at: 0 134 327 2403
Size: 134 193 2076
Fragments arranged by size:
2076 193 134
BtgI C'CryG_G
Cuts at: 0 425 693 2244 2403
Size: 425 268 1551 159
Fragments arranged by size:
1551 425 268 159
BtsI GCAGTG_nn'
Cuts at: 0 836 1064 2403
Size: 836 228 1339
Fragments arranged by size:
1339 836 228
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 209 244 2076 2111 2403
Size: 209 35 1832 35 292
Fragments arranged by size:
1832 292 209 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1086 2403
Size: 1086 1317
EaeI y'GGCC_r
Cuts at: 0 428 971 1605 2403
Size: 428 543 634 798
Fragments arranged by size:
798 634 543 428
EarI CTCTTCn'nnn_
Cuts at: 0 496 745 2403
Size: 496 249 1658
Fragments arranged by size:
1658 496 249
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1462 1596 2403
Size: 1462 134 807
Fragments arranged by size:
1462 807 134
EcoICRI GAG'CTC
Cuts at: 0 1020 1365 2403
Size: 1020 345 1038
Fragments arranged by size:
1038 1020 345
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 482 613 644 1275 2022 2235 2403
Size: 244 238 131 31 631 747 213 168
Fragments arranged by size:
747 631 244 238 213 168 131 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 785 804 2403
Size: 785 19 1599
Fragments arranged by size:
1599 785 19
EcoO109I rG'GnC_Cy
Cuts at: 0 80 650 2403
Size: 80 570 1753
Fragments arranged by size:
1753 570 80
EcoRI G'AATT_C
Cuts at: 0 934 1220 2403
Size: 934 286 1183
Fragments arranged by size:
1183 934 286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 196 2403
Size: 196 2207
HaeII r_GCGC'y
Cuts at: 0 822 1482 2403
Size: 822 660 921
Fragments arranged by size:
921 822 660
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 923 955 1315 1347 1384 1416 2157 2189
Size: 923 32 360 32 37 32 741 32
Cuts at: 2189 2403
Size: 214
Fragments arranged by size:
923 741 360 214 37 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 178 2403
Size: 178 2225
KasI G'GCGC_C
Cuts at: 0 1478 2403
Size: 1478 925
MfeI C'AATT_G
Cuts at: 0 1617 2403
Size: 1617 786
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 2403
Size: 146 2257
MscI TGG'CCA
Cuts at: 0 430 973 2403
Size: 430 543 1430
Fragments arranged by size:
1430 543 430
MslI CAynn'nnrTG
Cuts at: 0 424 553 557 692 2290 2403
Size: 424 129 4 135 1598 113
Fragments arranged by size:
1598 424 135 129 113 4
MspA1I CmG'CkG
Cuts at: 0 319 1388 1494 2307 2313 2403
Size: 319 1069 106 813 6 90
Fragments arranged by size:
1069 813 319 106 90 6
NaeI GCC'GGC
Cuts at: 0 1477 2403
Size: 1477 926
NarI GG'CG_CC
Cuts at: 0 1479 2403
Size: 1479 924
NcoI C'CATG_G
Cuts at: 0 425 2244 2403
Size: 425 1819 159
Fragments arranged by size:
1819 425 159
NgoMIV G'CCGG_C
Cuts at: 0 1475 2403
Size: 1475 928
NspI r_CATG'y
Cuts at: 0 546 669 2130 2403
Size: 546 123 1461 273
Fragments arranged by size:
1461 546 273 123
PciI A'CATG_T
Cuts at: 0 542 2126 2403
Size: 542 1584 277
Fragments arranged by size:
1584 542 277
PflMI CCAn_nnn'nTGG
Cuts at: 0 1086 2403
Size: 1086 1317
PmlI CAC'GTG
Cuts at: 0 1086 2403
Size: 1086 1317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1435 1467 2403
Size: 1435 32 936
Fragments arranged by size:
1435 936 32
PpuMI rG'GwC_Cy
Cuts at: 0 80 2403
Size: 80 2323
PstI C_TGCA'G
Cuts at: 0 1545 1703 2312 2403
Size: 1545 158 609 91
Fragments arranged by size:
1545 609 158 91
PvuI CG_AT'CG
Cuts at: 0 959 2403
Size: 959 1444
PvuII CAG'CTG
Cuts at: 0 1388 1494 2307 2313 2403
Size: 1388 106 813 6 90
Fragments arranged by size:
1388 813 106 90 6
SacI G_AGCT'C
Cuts at: 0 1022 1367 2403
Size: 1022 345 1036
Fragments arranged by size:
1036 1022 345
SanDI GG'GwC_CC
Cuts at: 0 80 2403
Size: 80 2323
SapI GCTCTTCn'nnn_
Cuts at: 0 496 2403
Size: 496 1907
SfcI C'TryA_G
Cuts at: 0 1541 1699 1858 2308 2403
Size: 1541 158 159 450 95
Fragments arranged by size:
1541 450 159 158 95
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 148 322 2403
Size: 148 174 2081
Fragments arranged by size:
2081 174 148
SfoI GGC'GCC
Cuts at: 0 1480 2403
Size: 1480 923
SgrAI Cr'CCGG_yG
Cuts at: 0 1475 2403
Size: 1475 928
SmlI C'TyrA_G
Cuts at: 0 768 2403
Size: 768 1635
StuI AGG'CCT
Cuts at: 0 151 1327 2403
Size: 151 1176 1076
Fragments arranged by size:
1176 1076 151
StyI C'CwwG_G
Cuts at: 0 425 452 1127 2244 2403
Size: 425 27 675 1117 159
Fragments arranged by size:
1117 675 425 159 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 559 2403
Size: 559 1844
TatI w'GTAC_w
Cuts at: 0 465 545 2403
Size: 465 80 1858
Fragments arranged by size:
1858 465 80
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2020 2094 2403
Size: 2020 74 309
Fragments arranged by size:
2020 309 74
ZraI GAC'GTC
Cuts at: 0 2069 2403
Size: 2069 334
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AleI AlfI AloI
AlwNI ApaLI ApoI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BglI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BstBI BstYI Bsu36I
BtgI BtsI CspCI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HindIII
KasI MfeI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmlI PpiI PpuMI
PstI PvuI PvuII SacI SanDI SapI SfcI SfiI
SfoI SgrAI SmlI StuI StyI TaqII TatI XcmI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI ApaI AscI
AseI AsiSI AvrII BcgI BciVI BclI BfrBI BglII
BlpI BmtI BsaWI BsiWI BspEI BspHI BssHII BssSI
BstAPI BstEII BstXI BstZ17I BtgZI ClaI DraI DrdI
EagI EcoRV FseI FspI FspAI HincII HpaI KpnI
MluI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmeI PshAI PsiI PspOMI PspXI PsrI RsrII
SacII SalI SbfI ScaI SexAI SgfI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI
XhoI XmaI XmnI