Restriction map for
pFN21AB5783
(Linear) (Six-base) MAPSORT of: KIBB5783.seq Check: 9478 from: 1 to: 2085
KIBB5783.orf
With 182 enzymes: SgfI *
July 3, 2012 16:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 855 1101 1344 2085
Size: 855 246 243 741
Fragments arranged by size:
855 741 246 243
Acc65I G'GTAC_C
Cuts at: 0 157 2085
Size: 157 1928
AclI AA'CG_TT
Cuts at: 0 551 2085
Size: 551 1534
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 1595 2005 2085
Size: 151 1444 410 80
Fragments arranged by size:
1444 410 151 80
AhdI GACnn_n'nnGTC
Cuts at: 0 824 2085
Size: 824 1261
AlwNI CAG_nnn'CTG
Cuts at: 0 1102 1450 1972 2085
Size: 1102 348 522 113
Fragments arranged by size:
1102 522 348 113
ApaI G_GGCC'C
Cuts at: 0 1461 1729 2085
Size: 1461 268 356
Fragments arranged by size:
1461 356 268
ApoI r'AATT_y
Cuts at: 0 514 1908 2085
Size: 514 1394 177
Fragments arranged by size:
1394 514 177
AvaI C'yCGr_G
Cuts at: 0 810 1081 1367 2085
Size: 810 271 286 718
Fragments arranged by size:
810 718 286 271
BanI G'GyrC_C
Cuts at: 0 157 2085
Size: 157 1928
BanII G_rGCy'C
Cuts at: 0 472 817 1461 1589 1694 1729 2085
Size: 472 345 644 128 105 35 356
Fragments arranged by size:
644 472 356 345 128 105 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 326 2085
Size: 326 1759
BbvCI CC'TCA_GC
Cuts at: 0 1373 1451 1781 2085
Size: 1373 78 330 304
Fragments arranged by size:
1373 330 304 78
BciVI GTATCCnnnnn_n'
Cuts at: 0 357 1215 2085
Size: 357 858 870
Fragments arranged by size:
870 858 357
BclI T'GATC_A
Cuts at: 0 1397 1974 2085
Size: 1397 577 111
Fragments arranged by size:
1397 577 111
BglI GCCn_nnn'nGGC
Cuts at: 0 721 1810 2085
Size: 721 1089 275
Fragments arranged by size:
1089 721 275
Bme1580I G_kGCm'C
Cuts at: 0 444 1461 1569 1729 2085
Size: 444 1017 108 160 356
Fragments arranged by size:
1017 444 356 160 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 709 740 2085
Size: 709 31 1345
Fragments arranged by size:
1345 709 31
BmtI G_CTAG'C
Cuts at: 0 1828 2085
Size: 1828 257
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1659 2085
Size: 1659 426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 612 1127 1427 2085
Size: 612 515 300 658
Fragments arranged by size:
658 612 515 300
Bpu10I CC'TnA_GC
Cuts at: 0 1373 1451 1781 2085
Size: 1373 78 330 304
Fragments arranged by size:
1373 330 304 78
BsaI GGTCTCn'nnnn_
Cuts at: 0 1693 2024 2085
Size: 1693 331 61
Fragments arranged by size:
1693 331 61
BsaWI w'CCGG_w
Cuts at: 0 1743 2085
Size: 1743 342
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 808 1057 1327 1390 1402 1537 1597 1709
Size: 808 249 270 63 12 135 60 112
Cuts at: 1709 2085
Size: 376
Fragments arranged by size:
808 376 270 249 135 112 63 60
12
BseYI C'CCAG_C
Cuts at: 0 81 2085
Size: 81 2004
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1577 1706 1781 2085
Size: 1577 129 75 304
Fragments arranged by size:
1577 304 129 75
BsiHKAI G_wGCw'C
Cuts at: 0 472 1589 2085
Size: 472 1117 496
Fragments arranged by size:
1117 496 472
BsmBI CGTCTCn'nnnn_
Cuts at: 0 630 857 2085
Size: 630 227 1228
Fragments arranged by size:
1228 630 227
Bsp1286I G_dGCh'C
Cuts at: 0 444 472 817 1461 1569 1589 1694 1729
Size: 444 28 345 644 108 20 105 35
Cuts at: 1729 2085
Size: 356
Fragments arranged by size:
644 444 356 345 108 105 35 28
20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 855 1087 1101 1344 2085
Size: 855 232 14 243 741
Fragments arranged by size:
855 741 243 232 14
BsrDI GCAATG_nn'
Cuts at: 0 767 1135 2085
Size: 767 368 950
Fragments arranged by size:
950 767 368
BsrFI r'CCGG_y
Cuts at: 0 1179 2085
Size: 1179 906
BssHII G'CGCG_C
Cuts at: 0 56 2085
Size: 56 2029
BssSI C'ACGA_G
Cuts at: 0 337 2085
Size: 337 1748
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1102 1564 2085
Size: 1102 462 521
Fragments arranged by size:
1102 521 462
BstBI TT'CG_AA
Cuts at: 0 116 2085
Size: 116 1969
BstEII G'GTnAC_C
Cuts at: 0 703 875 2085
Size: 703 172 1210
Fragments arranged by size:
1210 703 172
BstXI CCAn_nnnn'nTGG
Cuts at: 0 581 2085
Size: 581 1504
BstYI r'GATC_y
Cuts at: 0 1056 2085
Size: 1056 1029
Bsu36I CC'TnA_GG
Cuts at: 0 791 1028 1604 2085
Size: 791 237 576 481
Fragments arranged by size:
791 576 481 237
BtgI C'CryG_G
Cuts at: 0 1805 2085
Size: 1805 280
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 387 2085
Size: 387 1698
BtsI GCAGTG_nn'
Cuts at: 0 1400 1885 2085
Size: 1400 485 200
Fragments arranged by size:
1400 485 200
EaeI y'GGCC_r
Cuts at: 0 2 2085
Size: 2 2083
EarI CTCTTCn'nnn_
Cuts at: 0 536 560 1649 2085
Size: 536 24 1089 436
Fragments arranged by size:
1089 536 436 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1495 2085
Size: 1495 590
EcoICRI GAG'CTC
Cuts at: 0 470 1587 2085
Size: 470 1117 498
Fragments arranged by size:
1117 498 470
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 612 1127 1427 1595 2005 2085
Size: 151 461 515 300 168 410 80
Fragments arranged by size:
515 461 410 300 168 151 80
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1929 2085
Size: 1929 156
EcoO109I rG'GnC_Cy
Cuts at: 0 69 347 757 898 1146 1457 1458 1726
Size: 69 278 410 141 248 311 1 268
Cuts at: 1726 1776 2085
Size: 50 309
Fragments arranged by size:
410 311 309 278 268 248 141 69
50 1
HaeII r_GCGC'y
Cuts at: 0 745 2085
Size: 745 1340
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 559 591 810 842 2085
Size: 559 32 219 32 1243
Fragments arranged by size:
1243 559 219 32 32
HincII GTy'rAC
Cuts at: 0 555 2085
Size: 555 1530
KpnI G_GTAC'C
Cuts at: 0 161 2085
Size: 161 1924
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 1410 1670 2085
Size: 576 834 260 415
Fragments arranged by size:
834 576 415 260
MslI CAynn'nnrTG
Cuts at: 0 579 2085
Size: 579 1506
MspA1I CmG'CkG
Cuts at: 0 1426 1450 2085
Size: 1426 24 635
Fragments arranged by size:
1426 635 24
NaeI GCC'GGC
Cuts at: 0 1181 2085
Size: 1181 904
NcoI C'CATG_G
Cuts at: 0 1805 2085
Size: 1805 280
NgoMIV G'CCGG_C
Cuts at: 0 1179 2085
Size: 1179 906
NheI G'CTAG_C
Cuts at: 0 1824 2085
Size: 1824 261
NspI r_CATG'y
Cuts at: 0 210 2085
Size: 210 1875
PasI CC'CwG_GG
Cuts at: 0 1142 2085
Size: 1142 943
PflMI CCAn_nnn'nTGG
Cuts at: 0 81 453 2085
Size: 81 372 1632
Fragments arranged by size:
1632 372 81
PfoI T'CCnGG_A
Cuts at: 0 1772 2085
Size: 1772 313
PpuMI rG'GwC_Cy
Cuts at: 0 69 347 757 1146 1776 2085
Size: 69 278 410 389 630 309
Fragments arranged by size:
630 410 389 309 278 69
PspOMI G'GGCC_C
Cuts at: 0 1457 1725 2085
Size: 1457 268 360
Fragments arranged by size:
1457 360 268
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1910 1942 2085
Size: 1910 32 143
Fragments arranged by size:
1910 143 32
PstI C_TGCA'G
Cuts at: 0 467 947 1098 2085
Size: 467 480 151 987
Fragments arranged by size:
987 480 467 151
PvuII CAG'CTG
Cuts at: 0 1426 1450 2085
Size: 1426 24 635
Fragments arranged by size:
1426 635 24
SacI G_AGCT'C
Cuts at: 0 472 1589 2085
Size: 472 1117 496
Fragments arranged by size:
1117 496 472
SbfI CC_TGCA'GG
Cuts at: 0 1098 2085
Size: 1098 987
SfcI C'TryA_G
Cuts at: 0 463 943 1094 1679 2085
Size: 463 480 151 585 406
Fragments arranged by size:
585 480 463 406 151
SphI G_CATG'C
Cuts at: 0 210 2085
Size: 210 1875
StuI AGG'CCT
Cuts at: 0 217 724 1843 2051 2085
Size: 217 507 1119 208 34
Fragments arranged by size:
1119 507 217 208 34
StyI C'CwwG_G
Cuts at: 0 735 1353 1436 1694 1805 2085
Size: 735 618 83 258 111 280
Fragments arranged by size:
735 618 280 258 111 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 691 863 1137 1308 2085
Size: 691 172 274 171 777
Fragments arranged by size:
777 691 274 172 171
TatI w'GTAC_w
Cuts at: 0 2024 2085
Size: 2024 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1424 1456 2085
Size: 1424 32 629
Fragments arranged by size:
1424 629 32
XmnI GAAnn'nnTTC
Cuts at: 0 515 2085
Size: 515 1570
Enzymes that do cut:
AarI Acc65I AclI AcuI AhdI AlwNI ApaI ApoI
AvaI BanI BanII BbsI BbvCI BciVI BclI BglI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BsaI BsaWI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KpnI MmeI
MslI MspA1I NaeI NcoI NgoMIV NheI NspI PasI
PflMI PfoI PpuMI PspOMI PsrI PstI PvuII SacI
SbfI SfcI SphI StuI StyI TaqII TatI TstI
XmnI
Enzymes that do not cut:
AatII AccI AfeI AflII AflIII AgeI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BfrBI BglII BlpI BmgBI BpuEI BsaAI
BsaBI BsaHI BsaXI BsiEI BsiWI BsmI BspEI BspHI
BsrBI BsrGI BstZ17I ClaI CspCI DraI DraIII DrdI
EagI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI MfeI MluI MscI NarI NdeI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PvuI RsrII SacII SalI SanDI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XcmI
XhoI XmaI ZraI