Restriction map for
pFN21AB5781
(Linear) (Six-base) MAPSORT of: KIBB5781.seq Check: 5354 from: 1 to: 2478
KIBB5781.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1590 2478
Size: 1590 888
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 1694 2478
Size: 439 1255 784
Fragments arranged by size:
1255 784 439
AflIII A'CryG_T
Cuts at: 0 29 2478
Size: 29 2449
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1307 2478
Size: 1307 1171
AlwNI CAG_nnn'CTG
Cuts at: 0 370 409 2183 2478
Size: 370 39 1774 295
Fragments arranged by size:
1774 370 295 39
ApaI G_GGCC'C
Cuts at: 0 83 2478
Size: 83 2395
ApaLI G'TGCA_C
Cuts at: 0 1999 2471 2478
Size: 1999 472 7
Fragments arranged by size:
1999 472 7
ApoI r'AATT_y
Cuts at: 0 557 1201 1404 1416 1824 2478
Size: 557 644 203 12 408 654
Fragments arranged by size:
654 644 557 408 203 12
AseI AT'TA_AT
Cuts at: 0 1340 2478
Size: 1340 1138
AvaI C'yCGr_G
Cuts at: 0 176 2478
Size: 176 2302
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 506 539 1297 1330 2478
Size: 506 33 758 33 1148
Fragments arranged by size:
1148 758 506 33 33
BamHI G'GATC_C
Cuts at: 0 1188 2478
Size: 1188 1290
BanI G'GyrC_C
Cuts at: 0 6 2478
Size: 6 2472
BanII G_rGCy'C
Cuts at: 0 76 83 960 2478
Size: 76 7 877 1518
Fragments arranged by size:
1518 877 76 7
BbvCI CC'TCA_GC
Cuts at: 0 1749 2478
Size: 1749 729
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 167 201 2478
Size: 167 34 2277
Fragments arranged by size:
2277 167 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1926 2478
Size: 1926 552
BclI T'GATC_A
Cuts at: 0 582 1064 1224 2271 2478
Size: 582 482 160 1047 207
Fragments arranged by size:
1047 582 482 207 160
BglII A'GATC_T
Cuts at: 0 660 2478
Size: 660 1818
Bme1580I G_kGCm'C
Cuts at: 0 83 1697 2003 2475 2478
Size: 83 1614 306 472 3
Fragments arranged by size:
1614 472 306 83 3
BmgBI CAC'GTC
Cuts at: 0 107 2154 2478
Size: 107 2047 324
Fragments arranged by size:
2047 324 107
BmrI ACTGGGnnnn_n'
Cuts at: 0 276 861 2066 2182 2478
Size: 276 585 1205 116 296
Fragments arranged by size:
1205 585 296 276 116
BmtI G_CTAG'C
Cuts at: 0 838 2478
Size: 838 1640
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2115 2478
Size: 2115 363
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 90 260 844 944 1121 1382 2478
Size: 37 53 170 584 100 177 261 1096
Fragments arranged by size:
1096 584 261 177 170 100 53 37
Bpu10I CC'TnA_GC
Cuts at: 0 1749 2478
Size: 1749 729
BsaAI yAC'GTr
Cuts at: 0 30 2214 2478
Size: 30 2184 264
Fragments arranged by size:
2184 264 30
BsaBI GATnn'nnATC
Cuts at: 0 921 1472 2478
Size: 921 551 1006
Fragments arranged by size:
1006 921 551
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1478 1508 1617 1647 2478
Size: 1478 30 109 30 831
Fragments arranged by size:
1478 831 109 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 369 647 1579 2130 2478
Size: 369 278 932 551 348
Fragments arranged by size:
932 551 369 348 278
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1379 1840 2478
Size: 1379 461 638
Fragments arranged by size:
1379 638 461
BsiHKAI G_wGCw'C
Cuts at: 0 76 125 1457 2003 2123 2195 2475 2478
Size: 76 49 1332 546 120 72 280 3
Fragments arranged by size:
1332 546 280 120 76 72 49 3
Bsp1286I G_dGCh'C
Cuts at: 0 76 83 125 960 1457 1697 2003 2123
Size: 76 7 42 835 497 240 306 120
Cuts at: 2123 2195 2475 2478
Size: 72 280 3
Fragments arranged by size:
835 497 306 280 240 120 76 72
42 7 3
BspHI T'CATG_A
Cuts at: 0 843 2478
Size: 843 1635
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1590 2478
Size: 1590 888
BsrDI GCAATG_nn'
Cuts at: 0 549 1067 1340 2478
Size: 549 518 273 1138
Fragments arranged by size:
1138 549 518 273
BsrFI r'CCGG_y
Cuts at: 0 838 2478
Size: 838 1640
BsrGI T'GTAC_A
Cuts at: 0 379 2478
Size: 379 2099
BstAPI GCAn_nnn'nTGC
Cuts at: 0 110 409 2401 2478
Size: 110 299 1992 77
Fragments arranged by size:
1992 299 110 77
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1130 2478
Size: 1130 1348
BstYI r'GATC_y
Cuts at: 0 244 366 660 1188 2478
Size: 244 122 294 528 1290
Fragments arranged by size:
1290 528 294 244 122
BtgI C'CryG_G
Cuts at: 0 116 932 1083 1249 2478
Size: 116 816 151 166 1229
Fragments arranged by size:
1229 816 166 151 116
BtsI GCAGTG_nn'
Cuts at: 0 305 2376 2478
Size: 305 2071 102
Fragments arranged by size:
2071 305 102
DraI TTT'AAA
Cuts at: 0 293 464 2478
Size: 293 171 2014
Fragments arranged by size:
2014 293 171
DraIII CAC_nnn'GTG
Cuts at: 0 2445 2478
Size: 2445 33
EarI CTCTTCn'nnn_
Cuts at: 0 962 1249 1556 2478
Size: 962 287 307 922
Fragments arranged by size:
962 922 307 287
EcoICRI GAG'CTC
Cuts at: 0 74 2478
Size: 74 2404
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 90 260 439 844 944 1121 1382
Size: 37 53 170 179 405 100 177 261
Cuts at: 1382 1694 2478
Size: 312 784
Fragments arranged by size:
784 405 312 261 179 177 170 100
53 37
EcoO109I rG'GnC_Cy
Cuts at: 0 322 2478
Size: 322 2156
EcoRI G'AATT_C
Cuts at: 0 557 2478
Size: 557 1921
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2067 2265 2478
Size: 2067 198 213
Fragments arranged by size:
2067 213 198
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 351 383 955 987 1056 1088 1478 1510
Size: 351 32 572 32 69 32 390 32
Cuts at: 1510 1924 1956 2478
Size: 414 32 522
Fragments arranged by size:
572 522 414 390 351 69 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 725 1119 1574 2478
Size: 725 394 455 904
Fragments arranged by size:
904 725 455 394
HpaI GTT'AAC
Cuts at: 0 1119 1574 2478
Size: 1119 455 904
Fragments arranged by size:
1119 904 455
MfeI C'AATT_G
Cuts at: 0 183 2478
Size: 183 2295
MslI CAynn'nnrTG
Cuts at: 0 1163 1313 1700 1855 2478
Size: 1163 150 387 155 623
Fragments arranged by size:
1163 623 387 155 150
MspA1I CmG'CkG
Cuts at: 0 400 2173 2478
Size: 400 1773 305
Fragments arranged by size:
1773 400 305
NcoI C'CATG_G
Cuts at: 0 932 1249 2478
Size: 932 317 1229
Fragments arranged by size:
1229 932 317
NheI G'CTAG_C
Cuts at: 0 834 2478
Size: 834 1644
NspI r_CATG'y
Cuts at: 0 343 539 1780 2478
Size: 343 196 1241 698
Fragments arranged by size:
1241 698 343 196
PfoI T'CCnGG_A
Cuts at: 0 2147 2478
Size: 2147 331
PmlI CAC'GTG
Cuts at: 0 30 2478
Size: 30 2448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 58 90 2478
Size: 58 32 2388
Fragments arranged by size:
2388 58 32
PpuMI rG'GwC_Cy
Cuts at: 0 322 2478
Size: 322 2156
PshAI GACnn'nnGTC
Cuts at: 0 423 2173 2478
Size: 423 1750 305
Fragments arranged by size:
1750 423 305
PspOMI G'GGCC_C
Cuts at: 0 79 2478
Size: 79 2399
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 848 880 2478
Size: 848 32 1598
Fragments arranged by size:
1598 848 32
PstI C_TGCA'G
Cuts at: 0 399 405 1658 2478
Size: 399 6 1253 820
Fragments arranged by size:
1253 820 399 6
PvuII CAG'CTG
Cuts at: 0 400 2173 2478
Size: 400 1773 305
Fragments arranged by size:
1773 400 305
SacI G_AGCT'C
Cuts at: 0 76 2478
Size: 76 2402
ScaI AGT'ACT
Cuts at: 0 1581 2478
Size: 1581 897
SexAI A'CCwGG_T
Cuts at: 0 1909 2478
Size: 1909 569
SfcI C'TryA_G
Cuts at: 0 395 401 901 1654 2478
Size: 395 6 500 753 824
Fragments arranged by size:
824 753 500 395 6
SpeI A'CTAG_T
Cuts at: 0 1576 2478
Size: 1576 902
SphI G_CATG'C
Cuts at: 0 343 1780 2478
Size: 343 1437 698
Fragments arranged by size:
1437 698 343
SspI AAT'ATT
Cuts at: 0 530 1709 2478
Size: 530 1179 769
Fragments arranged by size:
1179 769 530
StuI AGG'CCT
Cuts at: 0 1299 2478
Size: 1299 1179
StyI C'CwwG_G
Cuts at: 0 932 1249 2478
Size: 932 317 1229
Fragments arranged by size:
1229 932 317
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2146 2478
Size: 2146 332
TatI w'GTAC_w
Cuts at: 0 379 1579 2478
Size: 379 1200 899
Fragments arranged by size:
1200 899 379
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2289 2321 2478
Size: 2289 32 157
Fragments arranged by size:
2289 157 32
Tth111I GACn'n_nGTC
Cuts at: 0 1180 2478
Size: 1180 1298
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 90 2478
Size: 90 2388
XmnI GAAnn'nnTTC
Cuts at: 0 992 1376 2478
Size: 992 384 1102
Fragments arranged by size:
1102 992 384
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApaI ApaLI ApoI
AseI AvaI BaeI BamHI BanI BanII BbvCI BcgI
BciVI BclI BglII Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BsaAI BsaBI BsaXI BseRI BsgI BsiHKAI
Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstAPI BstXI
BstYI BtgI BtsI DraI DraIII EarI EcoICRI Eco57MI
EcoO109I EcoRI FalI Hin4I HincII HpaI MfeI MslI
MspA1I NcoI NheI NspI PfoI PmlI PpiI PpuMI
PshAI PspOMI PsrI PstI PvuII SacI ScaI SexAI
SfcI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AscI AsiSI AvrII BbeI BbsI BfrBI
BglI BlpI BpuEI BsaI BsaHI BsaWI BseYI BsiEI
BsiWI BsmI BsmBI BspEI BsrBI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DrdI EaeI
EagI EciI EcoNI EcoRV FseI FspI FspAI HaeII
HindIII KasI KpnI MluI MmeI MscI NaeI NarI
NdeI NgoMIV NotI NruI NsiI PacI PasI PciI
PflMI PmeI PsiI PspXI PvuI RsrII SacII SalI
SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SrfI SwaI XbaI XhoI XmaI ZraI