Restriction map for
pFN21AB5770
(Linear) (Six-base) MAPSORT of: KIBB5770.seq Check: 153 from: 1 to: 2283
KIBB5770.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2009 2283
Size: 2009 274
AccI GT'mk_AC
Cuts at: 0 291 2283
Size: 291 1992
Acc65I G'GTAC_C
Cuts at: 0 1821 2283
Size: 1821 462
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 1276 2062 2283
Size: 48 1228 786 221
Fragments arranged by size:
1228 786 221 48
AflIII A'CryG_T
Cuts at: 0 677 1877 2283
Size: 677 1200 406
Fragments arranged by size:
1200 677 406
AhdI GACnn_n'nnGTC
Cuts at: 0 1911 2145 2283
Size: 1911 234 138
Fragments arranged by size:
1911 234 138
AlwNI CAG_nnn'CTG
Cuts at: 0 609 1117 2283
Size: 609 508 1166
Fragments arranged by size:
1166 609 508
ApoI r'AATT_y
Cuts at: 0 627 874 911 925 1304 1597 1620 1663
Size: 627 247 37 14 379 293 23 43
Cuts at: 1663 1846 2175 2283
Size: 183 329 108
Fragments arranged by size:
627 379 329 293 247 183 108 43
37 23 14
BanI G'GyrC_C
Cuts at: 0 1821 2283
Size: 1821 462
BanII G_rGCy'C
Cuts at: 0 1677 2283
Size: 1677 606
BbsI GAAGACnn'nnnn_
Cuts at: 0 1512 2283
Size: 1512 771
BclI T'GATC_A
Cuts at: 0 144 2283
Size: 144 2139
BfrBI ATG'CAT
Cuts at: 0 2035 2283
Size: 2035 248
BglII A'GATC_T
Cuts at: 0 719 2283
Size: 719 1564
BlpI GC'TnA_GC
Cuts at: 0 1919 2283
Size: 1919 364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 575 634 1483 2283
Size: 575 59 849 800
Fragments arranged by size:
849 800 575 59
BsaI GGTCTCn'nnnn_
Cuts at: 0 181 586 2283
Size: 181 405 1697
Fragments arranged by size:
1697 405 181
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1118 1148 2283
Size: 1118 30 1135
Fragments arranged by size:
1135 1118 30
BseYI C'CCAG_C
Cuts at: 0 418 2283
Size: 418 1865
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 1436 2283
Size: 12 1424 847
Fragments arranged by size:
1424 847 12
BsiEI CG_ry'CG
Cuts at: 0 1532 2283
Size: 1532 751
BsiHKAI G_wGCw'C
Cuts at: 0 43 824 1677 1754 2283
Size: 43 781 853 77 529
Fragments arranged by size:
853 781 529 77 43
BsmI GAATG_Cn'
Cuts at: 0 1554 2283
Size: 1554 729
Bsp1286I G_dGCh'C
Cuts at: 0 43 824 1677 1754 2283
Size: 43 781 853 77 529
Fragments arranged by size:
853 781 529 77 43
BspHI T'CATG_A
Cuts at: 0 1593 2283
Size: 1593 690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2009 2283
Size: 2009 274
BsrBI CCG'CTC
Cuts at: 0 2085 2283
Size: 2085 198
BsrDI GCAATG_nn'
Cuts at: 0 411 2035 2283
Size: 411 1624 248
Fragments arranged by size:
1624 411 248
BsrFI r'CCGG_y
Cuts at: 0 1531 2283
Size: 1531 752
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1623 2283
Size: 1623 660
BstYI r'GATC_y
Cuts at: 0 719 2283
Size: 719 1564
BtgI C'CryG_G
Cuts at: 0 73 2283
Size: 73 2210
BtsI GCAGTG_nn'
Cuts at: 0 607 1419 1424 2283
Size: 607 812 5 859
Fragments arranged by size:
859 812 607 5
ClaI AT'CG_AT
Cuts at: 0 165 1176 2283
Size: 165 1011 1107
Fragments arranged by size:
1107 1011 165
DrdI GACnn_nn'nnGTC
Cuts at: 0 2105 2283
Size: 2105 178
EaeI y'GGCC_r
Cuts at: 0 978 1392 1529 2283
Size: 978 414 137 754
Fragments arranged by size:
978 754 414 137
EagI C'GGCC_G
Cuts at: 0 1529 2283
Size: 1529 754
EarI CTCTTCn'nnn_
Cuts at: 0 31 422 467 632 812 1083 2283
Size: 31 391 45 165 180 271 1200
Fragments arranged by size:
1200 391 271 180 165 45 31
EcoICRI GAG'CTC
Cuts at: 0 1675 2283
Size: 1675 608
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 575 634 1276 1483 2062 2283
Size: 48 527 59 642 207 579 221
Fragments arranged by size:
642 579 527 221 207 59 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 63 868 2283
Size: 63 805 1415
Fragments arranged by size:
1415 805 63
EcoO109I rG'GnC_Cy
Cuts at: 0 78 2046 2283
Size: 78 1968 237
Fragments arranged by size:
1968 237 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1765 1797 1897 1929 1960 1992 2131 2163
Size: 1765 32 100 32 31 32 139 32
Cuts at: 2163 2283
Size: 120
Fragments arranged by size:
1765 139 120 100 32 32 32 32
31
HincII GTy'rAC
Cuts at: 0 675 2283
Size: 675 1608
HpaI GTT'AAC
Cuts at: 0 675 2283
Size: 675 1608
KpnI G_GTAC'C
Cuts at: 0 1825 2283
Size: 1825 458
MfeI C'AATT_G
Cuts at: 0 1148 2283
Size: 1148 1135
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 601 1838 1912 2283
Size: 74 527 1237 74 371
Fragments arranged by size:
1237 527 371 74 74
MscI TGG'CCA
Cuts at: 0 980 2283
Size: 980 1303
MspA1I CmG'CkG
Cuts at: 0 75 1918 2283
Size: 75 1843 365
Fragments arranged by size:
1843 365 75
NsiI A_TGCA'T
Cuts at: 0 2037 2283
Size: 2037 246
NspI r_CATG'y
Cuts at: 0 1881 2283
Size: 1881 402
PasI CC'CwG_GG
Cuts at: 0 1657 2283
Size: 1657 626
PciI A'CATG_T
Cuts at: 0 1877 2283
Size: 1877 406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1778 1810 2283
Size: 1778 32 473
Fragments arranged by size:
1778 473 32
PpuMI rG'GwC_Cy
Cuts at: 0 78 2046 2283
Size: 78 1968 237
Fragments arranged by size:
1968 237 78
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1202 1234 2283
Size: 1202 32 1049
Fragments arranged by size:
1202 1049 32
PstI C_TGCA'G
Cuts at: 0 1414 1548 2283
Size: 1414 134 735
Fragments arranged by size:
1414 735 134
PvuII CAG'CTG
Cuts at: 0 1918 2283
Size: 1918 365
SacI G_AGCT'C
Cuts at: 0 1677 2283
Size: 1677 606
SacII CC_GC'GG
Cuts at: 0 76 2283
Size: 76 2207
SanDI GG'GwC_CC
Cuts at: 0 78 2283
Size: 78 2205
SapI GCTCTTCn'nnn_
Cuts at: 0 31 812 1083 2283
Size: 31 781 271 1200
Fragments arranged by size:
1200 781 271 31
ScaI AGT'ACT
Cuts at: 0 1550 2283
Size: 1550 733
SfcI C'TryA_G
Cuts at: 0 111 201 1410 1544 2252 2283
Size: 111 90 1209 134 708 31
Fragments arranged by size:
1209 708 134 111 90 31
SpeI A'CTAG_T
Cuts at: 0 208 2283
Size: 208 2075
SspI AAT'ATT
Cuts at: 0 254 1368 1581 2283
Size: 254 1114 213 702
Fragments arranged by size:
1114 702 254 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 90 2283
Size: 90 2193
TatI w'GTAC_w
Cuts at: 0 1404 1548 2283
Size: 1404 144 735
Fragments arranged by size:
1404 735 144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 763 795 2283
Size: 763 32 1488
Fragments arranged by size:
1488 763 32
XbaI T'CTAG_A
Cuts at: 0 1033 2283
Size: 1033 1250
XmnI GAAnn'nnTTC
Cuts at: 0 641 2283
Size: 641 1642
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AlwNI ApoI
BanI BanII BbsI BclI BfrBI BglII BlpI BpmI
BsaI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BstXI BstYI BtgI
BtsI ClaI DrdI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I Hin4I HincII HpaI KpnI MfeI MmeI
MscI MspA1I NsiI NspI PasI PciI PpiI PpuMI
PsrI PstI PvuII SacI SacII SanDI SapI ScaI
SfcI SpeI SspI TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AleI AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BglI Bme1580I BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaWI BseRI BsiWI BsmBI BspEI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI CspCI DraI
DraIII EciI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HindIII KasI MluI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PflMI PfoI
PmeI PmlI PshAI PsiI PspOMI PspXI PvuI RsrII
SalI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SphI SrfI StuI StyI SwaI Tth111I
XcmI XhoI XmaI ZraI