Restriction map for
pFN21AB5767
(Linear) (Six-base) MAPSORT of: KIBB5767.seq Check: 1772 from: 1 to: 2436
KIBB5767.orf
With 182 enzymes: SgfI *
July 3, 2012 16:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 386 656 1953 2082 2436
Size: 386 270 1297 129 354
Fragments arranged by size:
1297 386 354 270 129
AatII G_ACGT'C
Cuts at: 0 89 590 2436
Size: 89 501 1846
Fragments arranged by size:
1846 501 89
AccI GT'mk_AC
Cuts at: 0 89 2436
Size: 89 2347
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 1906 2436
Size: 382 1524 530
Fragments arranged by size:
1524 530 382
AflIII A'CryG_T
Cuts at: 0 923 2436
Size: 923 1513
AlwNI CAG_nnn'CTG
Cuts at: 0 490 855 1173 1476 2436
Size: 490 365 318 303 960
Fragments arranged by size:
960 490 365 318 303
ApaI G_GGCC'C
Cuts at: 0 320 597 666 840 1215 1632 1997 2346
Size: 320 277 69 174 375 417 365 349
Cuts at: 2346 2436
Size: 90
Fragments arranged by size:
417 375 365 349 320 277 174 90
69
ApoI r'AATT_y
Cuts at: 0 732 1006 1162 2436
Size: 732 274 156 1274
Fragments arranged by size:
1274 732 274 156
AvaI C'yCGr_G
Cuts at: 0 538 833 947 1463 2436
Size: 538 295 114 516 973
Fragments arranged by size:
973 538 516 295 114
AvrII C'CTAG_G
Cuts at: 0 1221 2436
Size: 1221 1215
BamHI G'GATC_C
Cuts at: 0 1332 1427 2072 2436
Size: 1332 95 645 364
Fragments arranged by size:
1332 645 364 95
BanI G'GyrC_C
Cuts at: 0 1012 1387 1804 2184 2436
Size: 1012 375 417 380 252
Fragments arranged by size:
1012 417 380 375 252
BanII G_rGCy'C
Cuts at: 0 289 320 371 597 666 840 1215 1361
Size: 289 31 51 226 69 174 375 146
Cuts at: 1361 1632 1997 2171 2346 2436
Size: 271 365 174 175 90
Fragments arranged by size:
375 365 289 271 226 175 174 174
146 90 69 51 31
BbeI G_GCGC'C
Cuts at: 0 1016 1391 2436
Size: 1016 375 1045
Fragments arranged by size:
1045 1016 375
BbsI GAAGACnn'nnnn_
Cuts at: 0 1398 2436
Size: 1398 1038
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 529 563 814 848 2436
Size: 529 34 251 34 1588
Fragments arranged by size:
1588 529 251 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1527 2277 2436
Size: 1527 750 159
Fragments arranged by size:
1527 750 159
BglI GCCn_nnn'nGGC
Cuts at: 0 1992 2182 2436
Size: 1992 190 254
Fragments arranged by size:
1992 254 190
Bme1580I G_kGCm'C
Cuts at: 0 320 597 666 840 1215 1632 1997 2346
Size: 320 277 69 174 375 417 365 349
Cuts at: 2346 2436
Size: 90
Fragments arranged by size:
417 375 365 349 320 277 174 90
69
BmgBI CAC'GTC
Cuts at: 0 113 2436
Size: 113 2323
BmrI ACTGGGnnnn_n'
Cuts at: 0 323 1084 2436
Size: 323 761 1352
Fragments arranged by size:
1352 761 323
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1194 2436
Size: 1194 1242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 343 421 931 1147 1229 1561 1881
Size: 157 186 78 510 216 82 332 320
Cuts at: 1881 1948 2050 2274 2383 2436
Size: 67 102 224 109 53
Fragments arranged by size:
510 332 320 224 216 186 157 109
102 82 78 67 53
Bpu10I CC'TnA_GC
Cuts at: 0 2189 2436
Size: 2189 247
BsaI GGTCTCn'nnnn_
Cuts at: 0 907 2436
Size: 907 1529
BsaHI Gr'CG_yC
Cuts at: 0 86 587 1013 1388 2044 2436
Size: 86 501 426 375 656 392
Fragments arranged by size:
656 501 426 392 375 86
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 151 181 976 1006 1048 1078 1227 1246
Size: 151 30 795 30 42 30 149 19
Cuts at: 1246 1257 1276 2436
Size: 11 19 1160
Fragments arranged by size:
1160 795 151 149 42 30 30 30
19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 340 418 516 832 1267 2436
Size: 340 78 98 316 435 1169
Fragments arranged by size:
1169 435 340 316 98 78
BseYI C'CCAG_C
Cuts at: 0 618 963 1709 1909 1914 1918 2087 2436
Size: 618 345 746 200 5 4 169 349
Fragments arranged by size:
746 618 349 345 200 169 5 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 2436
Size: 634 1802
BsiHKAI G_wGCw'C
Cuts at: 0 2431 2436
Size: 2431 5
BsmI GAATG_Cn'
Cuts at: 0 381 2369 2436
Size: 381 1988 67
Fragments arranged by size:
1988 381 67
Bsp1286I G_dGCh'C
Cuts at: 0 289 320 371 597 666 840 1215 1361
Size: 289 31 51 226 69 174 375 146
Cuts at: 1361 1632 1997 2171 2346 2431 2436
Size: 271 365 174 175 85 5
Fragments arranged by size:
375 365 289 271 226 175 174 174
146 85 69 51 31 5
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 386 656 1523 1953 2082 2436
Size: 386 270 867 430 129 354
Fragments arranged by size:
867 430 386 354 270 129
BsrBI CCG'CTC
Cuts at: 0 330 2436
Size: 330 2106
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1524 2436
Size: 1524 912
BstEII G'GTnAC_C
Cuts at: 0 2295 2436
Size: 2295 141
BstXI CCAn_nnnn'nTGG
Cuts at: 0 219 313 618 2436
Size: 219 94 305 1818
Fragments arranged by size:
1818 305 219 94
BstYI r'GATC_y
Cuts at: 0 260 395 986 1332 1427 2072 2324 2436
Size: 260 135 591 346 95 645 252 112
Fragments arranged by size:
645 591 346 260 252 135 112 95
Bsu36I CC'TnA_GG
Cuts at: 0 241 896 1988 2066 2436
Size: 241 655 1092 78 370
Fragments arranged by size:
1092 655 370 241 78
BtgI C'CryG_G
Cuts at: 0 34 1051 1193 1301 1353 2171 2259 2436
Size: 34 1017 142 108 52 818 88 177
Fragments arranged by size:
1017 818 177 142 108 88 52 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1543 2436
Size: 1543 893
BtsI GCAGTG_nn'
Cuts at: 0 605 1291 1780 1858 2436
Size: 605 686 489 78 578
Fragments arranged by size:
686 605 578 489 78
DraIII CAC_nnn'GTG
Cuts at: 0 1002 2436
Size: 1002 1434
DrdI GACnn_nn'nnGTC
Cuts at: 0 1149 2436
Size: 1149 1287
EaeI y'GGCC_r
Cuts at: 0 122 494 1820 2174 2436
Size: 122 372 1326 354 262
Fragments arranged by size:
1326 372 354 262 122
EarI CTCTTCn'nnn_
Cuts at: 0 676 772 1055 1079 2436
Size: 676 96 283 24 1357
Fragments arranged by size:
1357 676 283 96 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 343 382 421 931 1147 1229 1561
Size: 157 186 39 39 510 216 82 332
Cuts at: 1561 1881 1906 1948 2050 2274 2383 2436
Size: 320 25 42 102 224 109 53
Fragments arranged by size:
510 332 320 224 216 186 157 109
102 82 53 42 39 39 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 175 2436
Size: 175 2261
EcoO109I rG'GnC_Cy
Cuts at: 0 30 74 317 594 662 663 837 884
Size: 30 44 243 277 68 1 174 47
Cuts at: 884 974 1122 1212 1276 1984 1993 2342 2436
Size: 90 148 90 64 708 9 349 94
Fragments arranged by size:
708 349 277 243 174 148 94 90
90 68 64 47 44 30 9 1
EcoRI G'AATT_C
Cuts at: 0 1162 2436
Size: 1162 1274
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 705 2436
Size: 705 1731
HaeII r_GCGC'y
Cuts at: 0 7 1016 1391 1731 2436
Size: 7 1009 375 340 705
Fragments arranged by size:
1009 705 375 340 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 151 183 753 785 1246 1278 1330 1362
Size: 151 32 570 32 461 32 52 32
Cuts at: 1362 1542 1574 2342 2374 2436
Size: 180 32 768 32 62
Fragments arranged by size:
768 570 461 180 151 62 52 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2154 2436
Size: 2154 282
KasI G'GCGC_C
Cuts at: 0 1012 1387 2436
Size: 1012 375 1049
Fragments arranged by size:
1049 1012 375
MscI TGG'CCA
Cuts at: 0 496 2436
Size: 496 1940
MslI CAynn'nnrTG
Cuts at: 0 212 2436
Size: 212 2224
MspA1I CmG'CkG
Cuts at: 0 36 852 1326 1659 1866 1918 1935 2436
Size: 36 816 474 333 207 52 17 501
Fragments arranged by size:
816 501 474 333 207 52 36 17
NarI GG'CG_CC
Cuts at: 0 1013 1388 2436
Size: 1013 375 1048
Fragments arranged by size:
1048 1013 375
NcoI C'CATG_G
Cuts at: 0 1193 2259 2436
Size: 1193 1066 177
Fragments arranged by size:
1193 1066 177
NdeI CA'TA_TG
Cuts at: 0 1382 2436
Size: 1382 1054
NspI r_CATG'y
Cuts at: 0 927 2160 2436
Size: 927 1233 276
Fragments arranged by size:
1233 927 276
PasI CC'CwG_GG
Cuts at: 0 151 597 2436
Size: 151 446 1839
Fragments arranged by size:
1839 446 151
PciI A'CATG_T
Cuts at: 0 923 2436
Size: 923 1513
PflMI CCAn_nnn'nTGG
Cuts at: 0 1173 1307 1476 2436
Size: 1173 134 169 960
Fragments arranged by size:
1173 960 169 134
PfoI T'CCnGG_A
Cuts at: 0 1115 1166 2360 2436
Size: 1115 51 1194 76
Fragments arranged by size:
1194 1115 76 51
PpuMI rG'GwC_Cy
Cuts at: 0 30 74 884 974 2436
Size: 30 44 810 90 1462
Fragments arranged by size:
1462 810 90 44 30
PspOMI G'GGCC_C
Cuts at: 0 316 593 662 836 1211 1628 1993 2342
Size: 316 277 69 174 375 417 365 349
Cuts at: 2342 2436
Size: 94
Fragments arranged by size:
417 375 365 349 316 277 174 94
69
PstI C_TGCA'G
Cuts at: 0 29 59 296 632 806 1109 1853 1871
Size: 29 30 237 336 174 303 744 18
Cuts at: 1871 2234 2436
Size: 363 202
Fragments arranged by size:
744 363 336 303 237 202 174 30
29 18
PvuII CAG'CTG
Cuts at: 0 852 1918 2436
Size: 852 1066 518
Fragments arranged by size:
1066 852 518
SacII CC_GC'GG
Cuts at: 0 37 2436
Size: 37 2399
SanDI GG'GwC_CC
Cuts at: 0 884 2436
Size: 884 1552
SapI GCTCTTCn'nnn_
Cuts at: 0 676 2436
Size: 676 1760
SbfI CC_TGCA'GG
Cuts at: 0 296 2234 2436
Size: 296 1938 202
Fragments arranged by size:
1938 296 202
SexAI A'CCwGG_T
Cuts at: 0 998 2436
Size: 998 1438
SfcI C'TryA_G
Cuts at: 0 25 55 292 628 802 1105 1666 1849
Size: 25 30 237 336 174 303 561 183
Cuts at: 1849 1867 2230 2436
Size: 18 363 206
Fragments arranged by size:
561 363 336 303 237 206 183 174
30 25 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1992 2436
Size: 1992 444
SfoI GGC'GCC
Cuts at: 0 1014 1389 2436
Size: 1014 375 1047
Fragments arranged by size:
1047 1014 375
SmaI CCC'GGG
Cuts at: 0 949 2436
Size: 949 1487
StuI AGG'CCT
Cuts at: 0 1646 2436
Size: 1646 790
StyI C'CwwG_G
Cuts at: 0 1193 1221 1373 1400 2259 2436
Size: 1193 28 152 27 859 177
Fragments arranged by size:
1193 859 177 152 28 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1101 1139 1723 2436
Size: 1101 38 584 713
Fragments arranged by size:
1101 713 584 38
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1048 1080 1227 1259 1734 1766 2436
Size: 1048 32 147 32 475 32 670
Fragments arranged by size:
1048 670 475 147 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 106 486 2436
Size: 106 380 1950
Fragments arranged by size:
1950 380 106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 318 1006 1380 1815 2178 2217 2436
Size: 318 688 374 435 363 39 219
Fragments arranged by size:
688 435 374 363 318 219 39
XmaI C'CCGG_G
Cuts at: 0 947 2436
Size: 947 1489
XmnI GAAnn'nnTTC
Cuts at: 0 1891 2436
Size: 1891 545
ZraI GAC'GTC
Cuts at: 0 87 588 2436
Size: 87 501 1848
Fragments arranged by size:
1848 501 87
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AlwNI ApaI ApoI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraIII DrdI EaeI EarI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI
MscI MslI MspA1I NarI NcoI NdeI NspI PasI
PciI PflMI PfoI PpuMI PspOMI PstI PvuII SacII
SanDI SapI SbfI SexAI SfcI SfiI SfoI SmaI
StuI StyI TaqII TstI Tth111I XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI
AloI ApaLI AscI AseI AsiSI BaeI BbvCI BclI
BfrBI BglII BlpI BmtI BpuEI BsaAI BsaBI BsaWI
BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstZ17I ClaI CspCI DraI EagI
EciI EcoICRI EcoRV FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MmeI NaeI NgoMIV NheI NotI
NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SalI ScaI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TatI XbaI XhoI