Restriction map for
pFN21AB5744
(Linear) (Six-base) MAPSORT of: KIBB5744.seq Check: 657 from: 1 to: 2238
KIBB5744.orf
With 182 enzymes: SgfI *
May 12, 2014 09:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2072 2127 2238
Size: 2072 55 111
Fragments arranged by size:
2072 111 55
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 1046 1345 1826 1992 2238
Size: 104 942 299 481 166 246
Fragments arranged by size:
942 481 299 246 166 104
AfeI AGC'GCT
Cuts at: 0 2122 2238
Size: 2122 116
AflIII A'CryG_T
Cuts at: 0 405 2238
Size: 405 1833
AhdI GACnn_n'nnGTC
Cuts at: 0 1055 1075 1638 2238
Size: 1055 20 563 600
Fragments arranged by size:
1055 600 563 20
AleI CACnn'nnGTG
Cuts at: 0 2147 2238
Size: 2147 91
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 452 484 1546 1578 2238
Size: 452 32 1062 32 660
Fragments arranged by size:
1062 660 452 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1029 2238
Size: 1029 1209
ApaI G_GGCC'C
Cuts at: 0 870 2238
Size: 870 1368
ApaLI G'TGCA_C
Cuts at: 0 2140 2238
Size: 2140 98
ApoI r'AATT_y
Cuts at: 0 545 1253 1317 2238
Size: 545 708 64 921
Fragments arranged by size:
921 708 545 64
AvaI C'yCGr_G
Cuts at: 0 960 2238
Size: 960 1278
BanI G'GyrC_C
Cuts at: 0 844 928 2238
Size: 844 84 1310
Fragments arranged by size:
1310 844 84
BanII G_rGCy'C
Cuts at: 0 352 870 1369 1837 2238
Size: 352 518 499 468 401
Fragments arranged by size:
518 499 468 401 352
BbvCI CC'TCA_GC
Cuts at: 0 1795 2238
Size: 1795 443
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2148 2182 2238
Size: 2148 34 56
Fragments arranged by size:
2148 56 34
BglI GCCn_nnn'nGGC
Cuts at: 0 448 1841 2238
Size: 448 1393 397
Fragments arranged by size:
1393 448 397
Bme1580I G_kGCm'C
Cuts at: 0 847 870 1222 2144 2238
Size: 847 23 352 922 94
Fragments arranged by size:
922 847 352 94 23
BmrI ACTGGGnnnn_n'
Cuts at: 0 873 1256 2238
Size: 873 383 982
Fragments arranged by size:
982 873 383
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 723 2238
Size: 723 1515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 911 1009 1510 1589 1719 2002 2238
Size: 911 98 501 79 130 283 236
Fragments arranged by size:
911 501 283 236 130 98 79
Bpu10I CC'TnA_GC
Cuts at: 0 884 1795 1927 2238
Size: 884 911 132 311
Fragments arranged by size:
911 884 311 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1354 1464 1551 2238
Size: 295 1059 110 87 687
Fragments arranged by size:
1059 687 295 110 87
BsaI GGTCTCn'nnnn_
Cuts at: 0 200 1878 2006 2238
Size: 200 1678 128 232
Fragments arranged by size:
1678 232 200 128
BsaWI w'CCGG_w
Cuts at: 0 1313 1550 2238
Size: 1313 237 688
Fragments arranged by size:
1313 688 237
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1749 2238
Size: 1749 489
BseYI C'CCAG_C
Cuts at: 0 1942 2238
Size: 1942 296
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1150 1485 2155 2238
Size: 1150 335 670 83
Fragments arranged by size:
1150 670 335 83
BsiEI CG_ry'CG
Cuts at: 0 1960 2238
Size: 1960 278
BsiHKAI G_wGCw'C
Cuts at: 0 352 885 1184 2144 2238
Size: 352 533 299 960 94
Fragments arranged by size:
960 533 352 299 94
BsmI GAATG_Cn'
Cuts at: 0 23 273 669 2238
Size: 23 250 396 1569
Fragments arranged by size:
1569 396 250 23
BsmBI CGTCTCn'nnnn_
Cuts at: 0 313 2238
Size: 313 1925
Bsp1286I G_dGCh'C
Cuts at: 0 352 847 870 885 1184 1222 1369 1837
Size: 352 495 23 15 299 38 147 468
Cuts at: 1837 2144 2238
Size: 307 94
Fragments arranged by size:
495 468 352 307 299 147 94 38
23 15
BspEI T'CCGG_A
Cuts at: 0 1313 1550 2238
Size: 1313 237 688
Fragments arranged by size:
1313 688 237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1860 2072 2127 2238
Size: 1860 212 55 111
Fragments arranged by size:
1860 212 111 55
BsrDI GCAATG_nn'
Cuts at: 0 1113 2238
Size: 1113 1125
BsrFI r'CCGG_y
Cuts at: 0 931 1497 2238
Size: 931 566 741
Fragments arranged by size:
931 741 566
BsrGI T'GTAC_A
Cuts at: 0 93 767 2238
Size: 93 674 1471
Fragments arranged by size:
1471 674 93
BssSI C'ACGA_G
Cuts at: 0 463 493 2238
Size: 463 30 1745
Fragments arranged by size:
1745 463 30
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1029 2238
Size: 1029 1209
BstEII G'GTnAC_C
Cuts at: 0 397 556 2238
Size: 397 159 1682
Fragments arranged by size:
1682 397 159
BstYI r'GATC_y
Cuts at: 0 13 467 692 2238
Size: 13 454 225 1546
Fragments arranged by size:
1546 454 225 13
BtgI C'CryG_G
Cuts at: 0 968 2238
Size: 968 1270
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2193 2238
Size: 2193 45
BtsI GCAGTG_nn'
Cuts at: 0 1030 1473 2154 2238
Size: 1030 443 681 84
Fragments arranged by size:
1030 681 443 84
DraIII CAC_nnn'GTG
Cuts at: 0 221 2238
Size: 221 2017
EaeI y'GGCC_r
Cuts at: 0 429 1495 1996 2238
Size: 429 1066 501 242
Fragments arranged by size:
1066 501 429 242
EarI CTCTTCn'nnn_
Cuts at: 0 968 2238
Size: 968 1270
EcoICRI GAG'CTC
Cuts at: 0 350 2238
Size: 350 1888
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 911 1009 1046 1345 1510 1589 1719
Size: 104 807 98 37 299 165 79 130
Cuts at: 1719 1826 1992 2002 2238
Size: 107 166 10 236
Fragments arranged by size:
807 299 236 166 165 130 107 104
98 79 37 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1213 1534 1930 2238
Size: 1213 321 396 308
Fragments arranged by size:
1213 396 321 308
EcoO109I rG'GnC_Cy
Cuts at: 0 1975 2238
Size: 1975 263
EcoRV GAT'ATC
Cuts at: 0 1785 2238
Size: 1785 453
HaeII r_GCGC'y
Cuts at: 0 2124 2238
Size: 2124 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 60 92 291 323 610 642 1041 1061
Size: 60 32 199 32 287 32 399 20
Cuts at: 1061 1073 1093 1624 1656 2238
Size: 12 20 531 32 582
Fragments arranged by size:
582 531 399 287 199 60 32 32
32 32 20 20 12
HincII GTy'rAC
Cuts at: 0 1406 2238
Size: 1406 832
HindIII A'AGCT_T
Cuts at: 0 1357 2238
Size: 1357 881
HpaI GTT'AAC
Cuts at: 0 1406 2238
Size: 1406 832
MfeI C'AATT_G
Cuts at: 0 771 1158 2238
Size: 771 387 1080
Fragments arranged by size:
1080 771 387
MscI TGG'CCA
Cuts at: 0 431 1998 2238
Size: 431 1567 240
Fragments arranged by size:
1567 431 240
MslI CAynn'nnrTG
Cuts at: 0 1768 2053 2147 2238
Size: 1768 285 94 91
Fragments arranged by size:
1768 285 94 91
MspA1I CmG'CkG
Cuts at: 0 61 970 2238
Size: 61 909 1268
Fragments arranged by size:
1268 909 61
NaeI GCC'GGC
Cuts at: 0 933 2238
Size: 933 1305
NgoMIV G'CCGG_C
Cuts at: 0 931 2238
Size: 931 1307
NspI r_CATG'y
Cuts at: 0 409 478 2101 2238
Size: 409 69 1623 137
Fragments arranged by size:
1623 409 137 69
PciI A'CATG_T
Cuts at: 0 405 2238
Size: 405 1833
PflMI CCAn_nnn'nTGG
Cuts at: 0 408 2238
Size: 408 1830
PfoI T'CCnGG_A
Cuts at: 0 1487 2238
Size: 1487 751
PspOMI G'GGCC_C
Cuts at: 0 866 2238
Size: 866 1372
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 751 783 1644 1676 2238
Size: 751 32 861 32 562
Fragments arranged by size:
861 751 562 32 32
PstI C_TGCA'G
Cuts at: 0 150 2083 2238
Size: 150 1933 155
Fragments arranged by size:
1933 155 150
PvuII CAG'CTG
Cuts at: 0 61 2238
Size: 61 2177
RsrII CG'GwC_CG
Cuts at: 0 510 2238
Size: 510 1728
SacI G_AGCT'C
Cuts at: 0 352 2238
Size: 352 1886
SacII CC_GC'GG
Cuts at: 0 971 2238
Size: 971 1267
ScaI AGT'ACT
Cuts at: 0 2005 2238
Size: 2005 233
SexAI A'CCwGG_T
Cuts at: 0 217 2238
Size: 217 2021
SfcI C'TryA_G
Cuts at: 0 146 2079 2238
Size: 146 1933 159
Fragments arranged by size:
1933 159 146
SmlI C'TyrA_G
Cuts at: 0 310 1333 1443 1566 2238
Size: 310 1023 110 123 672
Fragments arranged by size:
1023 672 310 123 110
SphI G_CATG'C
Cuts at: 0 478 2238
Size: 478 1760
StuI AGG'CCT
Cuts at: 0 1844 2238
Size: 1844 394
StyI C'CwwG_G
Cuts at: 0 432 1839 1854 2230 2238
Size: 432 1407 15 376 8
Fragments arranged by size:
1407 432 376 15 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 368 1804 2238
Size: 368 1436 434
Fragments arranged by size:
1436 434 368
TatI w'GTAC_w
Cuts at: 0 93 767 805 2003 2238
Size: 93 674 38 1198 235
Fragments arranged by size:
1198 674 235 93 38
Tth111I GACn'n_nGTC
Cuts at: 0 1621 2238
Size: 1621 617
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 218 2238
Size: 218 2020
XmnI GAAnn'nnTTC
Cuts at: 0 1059 2238
Size: 1059 1179
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AloI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbvCI BcgI
BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrDI BsrFI BsrGI BssSI BstAPI
BstEII BstYI BtgI BtgZI BtsI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HincII
HindIII HpaI MfeI MscI MslI MspA1I NaeI NgoMIV
NspI PciI PflMI PfoI PspOMI PsrI PstI PvuII
RsrII SacI SacII ScaI SexAI SfcI SmlI SphI
StuI StyI TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AlfI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI BsaAI BsaBI
BsaHI BsaXI BsiWI BspHI BsrBI BssHII BstBI BstXI
BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EciI
EcoRI FalI FseI FspI FspAI KasI KpnI MluI
MmeI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PvuI SalI SanDI SapI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
TstI XbaI XhoI XmaI ZraI