Restriction map for
pFN21AB5728
(Linear) (Six-base) MAPSORT of: KIBB5728.seq Check: 265 from: 1 to: 2193
KIBB5728.orf
With 182 enzymes: SgfI *
March 22, 2012 15:31 ..
AccI GT'mk_AC
Cuts at: 0 1624 2193
Size: 1624 569
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1574 2193
Size: 1574 619
AfeI AGC'GCT
Cuts at: 0 137 2193
Size: 137 2056
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 595 627 2193
Size: 595 32 1566
Fragments arranged by size:
1566 595 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1597 2193
Size: 1597 596
ApaI G_GGCC'C
Cuts at: 0 190 2193
Size: 190 2003
ApoI r'AATT_y
Cuts at: 0 796 1171 1282 1684 2092 2193
Size: 796 375 111 402 408 101
Fragments arranged by size:
796 408 402 375 111 101
AvaI C'yCGr_G
Cuts at: 0 46 171 181 2193
Size: 46 125 10 2012
Fragments arranged by size:
2012 125 46 10
AvrII C'CTAG_G
Cuts at: 0 315 2193
Size: 315 1878
BamHI G'GATC_C
Cuts at: 0 1572 2193
Size: 1572 621
BanI G'GyrC_C
Cuts at: 0 210 2193
Size: 210 1983
BanII G_rGCy'C
Cuts at: 0 31 190 368 2193
Size: 31 159 178 1825
Fragments arranged by size:
1825 178 159 31
BbeI G_GCGC'C
Cuts at: 0 214 2193
Size: 214 1979
BbsI GAAGACnn'nnnn_
Cuts at: 0 1100 2193
Size: 1100 1093
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 381 415 2193
Size: 381 34 1778
Fragments arranged by size:
1778 381 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 560 2193
Size: 560 1633
BglI GCCn_nnn'nGGC
Cuts at: 0 185 2193
Size: 185 2008
Bme1580I G_kGCm'C
Cuts at: 0 190 2121 2193
Size: 190 1931 72
Fragments arranged by size:
1931 190 72
BmtI G_CTAG'C
Cuts at: 0 1463 2193
Size: 1463 730
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 986 1337 2133 2193
Size: 294 692 351 796 60
Fragments arranged by size:
796 692 351 294 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 787 2193
Size: 787 1406
BsaHI Gr'CG_yC
Cuts at: 0 211 2193
Size: 211 1982
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 54 72 334 2193
Size: 54 18 262 1859
Fragments arranged by size:
1859 262 54 18
BseYI C'CCAG_C
Cuts at: 0 34 283 2193
Size: 34 249 1910
Fragments arranged by size:
1910 249 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 1745 2193
Size: 757 988 448
Fragments arranged by size:
988 757 448
BsmI GAATG_Cn'
Cuts at: 0 1186 2193
Size: 1186 1007
Bsp1286I G_dGCh'C
Cuts at: 0 31 190 368 2121 2193
Size: 31 159 178 1753 72
Fragments arranged by size:
1753 178 159 72 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1411 2193
Size: 1411 782
BsrFI r'CCGG_y
Cuts at: 0 166 289 2193
Size: 166 123 1904
Fragments arranged by size:
1904 166 123
BstBI TT'CG_AA
Cuts at: 0 1411 2193
Size: 1411 782
BstYI r'GATC_y
Cuts at: 0 842 1572 2193
Size: 842 730 621
Fragments arranged by size:
842 730 621
BtgI C'CryG_G
Cuts at: 0 116 248 2193
Size: 116 132 1945
Fragments arranged by size:
1945 132 116
BtsI GCAGTG_nn'
Cuts at: 0 1111 1376 1470 2193
Size: 1111 265 94 723
Fragments arranged by size:
1111 723 265 94
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 445 480 2193
Size: 445 35 1713
Fragments arranged by size:
1713 445 35
DraIII CAC_nnn'GTG
Cuts at: 0 630 2193
Size: 630 1563
EaeI y'GGCC_r
Cuts at: 0 275 453 645 704 2193
Size: 275 178 192 59 1489
Fragments arranged by size:
1489 275 192 178 59
EarI CTCTTCn'nnn_
Cuts at: 0 102 863 1534 1701 2193
Size: 102 761 671 167 492
Fragments arranged by size:
761 671 492 167 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 66 2193
Size: 66 2127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 986 1337 1574 2133 2193
Size: 294 692 351 237 559 60
Fragments arranged by size:
692 559 351 294 237 60
EcoO109I rG'GnC_Cy
Cuts at: 0 50 186 2193
Size: 50 136 2007
Fragments arranged by size:
2007 136 50
EcoRI G'AATT_C
Cuts at: 0 796 2193
Size: 796 1397
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 712 2193
Size: 712 1481
HaeII r_GCGC'y
Cuts at: 0 139 214 290 2193
Size: 139 75 76 1903
Fragments arranged by size:
1903 139 76 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 329 361 2119 2151 2193
Size: 329 32 1758 32 42
Fragments arranged by size:
1758 329 42 32 32
HincII GTy'rAC
Cuts at: 0 1557 2193
Size: 1557 636
KasI G'GCGC_C
Cuts at: 0 210 2193
Size: 210 1983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 1145 1411 1528 2193
Size: 704 441 266 117 665
Fragments arranged by size:
704 665 441 266 117
MscI TGG'CCA
Cuts at: 0 455 647 706 2193
Size: 455 192 59 1487
Fragments arranged by size:
1487 455 192 59
MslI CAynn'nnrTG
Cuts at: 0 1745 1919 2193
Size: 1745 174 274
Fragments arranged by size:
1745 274 174
MspA1I CmG'CkG
Cuts at: 0 34 269 323 1421 2193
Size: 34 235 54 1098 772
Fragments arranged by size:
1098 772 235 54 34
NaeI GCC'GGC
Cuts at: 0 168 291 2193
Size: 168 123 1902
Fragments arranged by size:
1902 168 123
NarI GG'CG_CC
Cuts at: 0 211 2193
Size: 211 1982
NdeI CA'TA_TG
Cuts at: 0 1236 2193
Size: 1236 957
NgoMIV G'CCGG_C
Cuts at: 0 166 289 2193
Size: 166 123 1904
Fragments arranged by size:
1904 166 123
NheI G'CTAG_C
Cuts at: 0 1459 2193
Size: 1459 734
NspI r_CATG'y
Cuts at: 0 772 2193
Size: 772 1421
PfoI T'CCnGG_A
Cuts at: 0 1361 2193
Size: 1361 832
PpuMI rG'GwC_Cy
Cuts at: 0 50 2193
Size: 50 2143
PshAI GACnn'nnGTC
Cuts at: 0 1512 2193
Size: 1512 681
PspOMI G'GGCC_C
Cuts at: 0 186 2193
Size: 186 2007
PvuII CAG'CTG
Cuts at: 0 269 1421 2193
Size: 269 1152 772
Fragments arranged by size:
1152 772 269
SfcI C'TryA_G
Cuts at: 0 781 2193
Size: 781 1412
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 185 2193
Size: 185 2008
SfoI GGC'GCC
Cuts at: 0 212 2193
Size: 212 1981
SgrAI Cr'CCGG_yG
Cuts at: 0 289 2193
Size: 289 1904
SmaI CCC'GGG
Cuts at: 0 48 2193
Size: 48 2145
SmlI C'TyrA_G
Cuts at: 0 802 2193
Size: 802 1391
SpeI A'CTAG_T
Cuts at: 0 732 2193
Size: 732 1461
SspI AAT'ATT
Cuts at: 0 537 2193
Size: 537 1656
StuI AGG'CCT
Cuts at: 0 1940 2193
Size: 1940 253
StyI C'CwwG_G
Cuts at: 0 315 1039 2193
Size: 315 724 1154
Fragments arranged by size:
1154 724 315
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2084 2193
Size: 2084 109
TatI w'GTAC_w
Cuts at: 0 1997 2193
Size: 1997 196
Tth111I GACn'n_nGTC
Cuts at: 0 1354 2193
Size: 1354 839
XbaI T'CTAG_A
Cuts at: 0 1632 2193
Size: 1632 561
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1707 2193
Size: 1707 486
XmaI C'CCGG_G
Cuts at: 0 46 2193
Size: 46 2147
XmnI GAAnn'nnTTC
Cuts at: 0 575 2193
Size: 575 1618
Enzymes that do cut:
AccI AcuI AfeI AloI AlwNI ApaI ApoI AvaI
AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI
BglI Bme1580I BmtI BpmI BpuEI BsaHI BseRI BseYI
BsgI BsmI Bsp1286I BspMI BsrFI BstBI BstYI BtgI
BtsI CspCI DraIII EaeI EarI EciI Eco57MI EcoO109I
EcoRI FalI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NheI NspI
PfoI PpuMI PshAI PspOMI PvuII SfcI SfiI SfoI
SgrAI SmaI SmlI SpeI SspI StuI StyI TaqII
TatI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI ApaLI AscI AseI AsiSI BaeI BbvCI
BclI BfrBI BglII BlpI BmgBI BmrI BplI Bpu10I
BsaI BsaAI BsaBI BsaWI BsaXI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraI
DrdI EagI EcoICRI EcoNI EcoRV FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI NcoI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PsiI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SgfI SnaBI
SphI SrfI SwaI TstI XhoI ZraI