(Linear) (Six-base) MAPSORT of: KIBB5724.seq  Check: 1006  from: 1  to: 2187



KIBB5724.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:29  ..

AclI AA'CG_TT

Cuts at:      0    2174    2187 
   Size:       2174      13
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     802    1540    2187 
   Size:        802     738     647

  Fragments arranged by size: 

                 802     738     647
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1075    2187 
   Size:       1075    1112
 
AlwNI CAG_nnn'CTG

Cuts at:      0     328     646    2187 
   Size:        328     318    1541

  Fragments arranged by size: 

                1541     328     318
 
ApaLI G'TGCA_C

Cuts at:      0     394    2187 
   Size:        394    1793
 
ApoI r'AATT_y

Cuts at:      0     163     259     331    1187    1490    1750    2187 
   Size:        163      96      72     856     303     260     437

  Fragments arranged by size: 

                 856     437     303     260     163      96      72
 
AseI AT'TA_AT

Cuts at:      0    1174    1312    1345    2187 
   Size:       1174     138      33     842

  Fragments arranged by size: 

                1174     842     138      33
 
AvaI C'yCGr_G

Cuts at:      0     809    2187 
   Size:        809    1378
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      84     808    1546    2187 
   Size:         84     724     738     641

  Fragments arranged by size: 

                 738     724     641      84
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1319    2187 
   Size:       1319     868
 
BclI T'GATC_A

Cuts at:      0    1394    2187 
   Size:       1394     793
 
Bme1580I G_kGCm'C

Cuts at:      0     398    1611    2187 
   Size:        398    1213     576

  Fragments arranged by size: 

                1213     576     398
 
BmtI G_CTAG'C

Cuts at:      0    1238    2187 
   Size:       1238     949
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     761     795    1565    2187 
   Size:        761      34     770     622

  Fragments arranged by size: 

                 770     761     622      34
 
Bpu10I CC'TnA_GC

Cuts at:      0    1244    2187 
   Size:       1244     943
 
BsaBI GATnn'nnATC

Cuts at:      0    1459    2187 
   Size:       1459     728
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     587     617    2187 
   Size:        587      30    1570

  Fragments arranged by size: 

                1570     587      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     238    2187 
   Size:        238    1949
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     497    2187 
   Size:        497    1690
 
BsiHKAI G_wGCw'C

Cuts at:      0      10     398    2187 
   Size:         10     388    1789

  Fragments arranged by size: 

                1789     388      10
 
Bsp1286I G_dGCh'C

Cuts at:      0      10     398    1611    2187 
   Size:         10     388    1213     576

  Fragments arranged by size: 

                1213     576     388      10
 
BspHI T'CATG_A

Cuts at:      0    1005    2187 
   Size:       1005    1182
 
BsrGI T'GTAC_A

Cuts at:      0     455    2187 
   Size:        455    1732
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     773    2187 
   Size:        773    1414
 
BtgI C'CryG_G

Cuts at:      0    2153    2187 
   Size:       2153      34
 
BtsI GCAGTG_nn'

Cuts at:      0     569     652    2187 
   Size:        569      83    1535

  Fragments arranged by size: 

                1535     569      83
 
EarI CTCTTCn'nnn_

Cuts at:      0     215    2078    2187 
   Size:        215    1863     109

  Fragments arranged by size: 

                1863     215     109
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     761     795     802    1540    1565    2187 
   Size:        761      34       7     738      25     622

  Fragments arranged by size: 

                 761     738     622      34      25       7
 
EcoO109I rG'GnC_Cy

Cuts at:      0     676    2187 
   Size:        676    1511
 
EcoRI G'AATT_C

Cuts at:      0     331    2187 
   Size:        331    1856
 
EcoRV GAT'ATC

Cuts at:      0     405    1563    2187 
   Size:        405    1158     624

  Fragments arranged by size: 

                1158     624     405
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     433     465    1061    1093    1674    1706    1857    1889 
   Size:        433      32     596      32     581      32     151      32

Cuts at:   1889    2187 
   Size:        298

  Fragments arranged by size: 

                 596     581     433     298     151      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    2178    2187 
   Size:       2178       9
 
HindIII A'AGCT_T

Cuts at:      0     129    2187 
   Size:        129    2058
 
MfeI C'AATT_G

Cuts at:      0     767    2187 
   Size:        767    1420
 
NcoI C'CATG_G

Cuts at:      0    2153    2187 
   Size:       2153      34
 
NheI G'CTAG_C

Cuts at:      0    1234    2187 
   Size:       1234     953
 
NspI r_CATG'y

Cuts at:      0     789    2187 
   Size:        789    1398
 
PacI TTA_AT'TAA

Cuts at:      0    1349    2187 
   Size:       1349     838
 
PsiI TTA'TAA

Cuts at:      0    1730    2187 
   Size:       1730     457
 
PstI C_TGCA'G

Cuts at:      0    1279    2187 
   Size:       1279     908
 
ScaI AGT'ACT

Cuts at:      0     248    2187 
   Size:        248    1939
 
SfcI C'TryA_G

Cuts at:      0    1275    2187 
   Size:       1275     912
 
SpeI A'CTAG_T

Cuts at:      0     904    2187 
   Size:        904    1283
 
SspI AAT'ATT

Cuts at:      0     198     535    2187 
   Size:        198     337    1652

  Fragments arranged by size: 

                1652     337     198
 
StyI C'CwwG_G

Cuts at:      0      48    2153    2187 
   Size:         48    2105      34

  Fragments arranged by size: 

                2105      48      34
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     349    2187 
   Size:        349    1838
 
TatI w'GTAC_w

Cuts at:      0     246     455     744    1203    2187 
   Size:        246     209     289     459     984

  Fragments arranged by size: 

                 984     459     289     246     209
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     437     469    1091    1123    2187 
   Size:        437      32     622      32    1064

  Fragments arranged by size: 

                1064     622     437      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     643    2187 
   Size:        643    1544
 
XmnI GAAnn'nnTTC

Cuts at:      0     723    1370    2187 
   Size:        723     647     817

  Fragments arranged by size: 

                 817     723     647
 

 Enzymes that do cut:

     AclI     AcuI     AhdI    AlwNI    ApaLI     ApoI     AseI     AvaI
     BbsI    BciVI     BclI Bme1580I     BmtI     BpmI   Bpu10I    BsaBI
    BsaXI    BseRI     BsgI  BsiHKAI Bsp1286I    BspHI    BsrGI    BstXI
     BtgI     BtsI     EarI  Eco57MI EcoO109I    EcoRI    EcoRV    Hin4I
   HincII  HindIII     MfeI     NcoI     NheI     NspI     PacI     PsiI
     PstI     ScaI     SfcI     SpeI     SspI     StyI    TaqII     TatI
     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI     ApaI     AscI    AsiSI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BplI    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI     FalI
     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NotI     NruI     NsiI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SphI     SrfI     StuI     SwaI  Tth111I     XbaI     XhoI     XmaI
     ZraI