Restriction map for
pFN21AB5690
(Linear) (Six-base) MAPSORT of: KIBB5690.seq Check: 4728 from: 1 to: 1554
KIBB5690.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1300 1554
Size: 1300 254
AatII G_ACGT'C
Cuts at: 0 434 1554
Size: 434 1120
AccI GT'mk_AC
Cuts at: 0 242 793 1554
Size: 242 551 761
Fragments arranged by size:
761 551 242
AclI AA'CG_TT
Cuts at: 0 677 1554
Size: 677 877
AflIII A'CryG_T
Cuts at: 0 380 1554
Size: 380 1174
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 937 1554
Size: 937 617
AlwNI CAG_nnn'CTG
Cuts at: 0 1315 1324 1367 1554
Size: 1315 9 43 187
Fragments arranged by size:
1315 187 43 9
ApoI r'AATT_y
Cuts at: 0 552 830 991 1038 1328 1554
Size: 552 278 161 47 290 226
Fragments arranged by size:
552 290 278 226 161 47
AvaI C'yCGr_G
Cuts at: 0 14 138 145 1554
Size: 14 124 7 1409
Fragments arranged by size:
1409 124 14 7
BanI G'GyrC_C
Cuts at: 0 217 913 1222 1554
Size: 217 696 309 332
Fragments arranged by size:
696 332 309 217
BanII G_rGCy'C
Cuts at: 0 1266 1294 1397 1554
Size: 1266 28 103 157
Fragments arranged by size:
1266 157 103 28
BbeI G_GCGC'C
Cuts at: 0 221 1554
Size: 221 1333
BbsI GAAGACnn'nnnn_
Cuts at: 0 485 1554
Size: 485 1069
BbvCI CC'TCA_GC
Cuts at: 0 1130 1554
Size: 1130 424
BmrI ACTGGGnnnn_n'
Cuts at: 0 36 1022 1554
Size: 36 986 532
Fragments arranged by size:
986 532 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1462 1554
Size: 1462 92
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 301 652 1083 1554
Size: 211 90 351 431 471
Fragments arranged by size:
471 431 351 211 90
Bpu10I CC'TnA_GC
Cuts at: 0 1130 1554
Size: 1130 424
BsaI GGTCTCn'nnnn_
Cuts at: 0 628 1554
Size: 628 926
BsaAI yAC'GTr
Cuts at: 0 383 1554
Size: 383 1171
BsaBI GATnn'nnATC
Cuts at: 0 289 1554
Size: 289 1265
BsaHI Gr'CG_yC
Cuts at: 0 205 218 431 1554
Size: 205 13 213 1123
Fragments arranged by size:
1123 213 205 13
BsaWI w'CCGG_w
Cuts at: 0 1481 1554
Size: 1481 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 667 697 1554
Size: 667 30 857
Fragments arranged by size:
857 667 30
BseYI C'CCAG_C
Cuts at: 0 1019 1554
Size: 1019 535
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1319 1554
Size: 1319 235
Bsp1286I G_dGCh'C
Cuts at: 0 1266 1294 1397 1554
Size: 1266 28 103 157
Fragments arranged by size:
1266 157 103 28
BspHI T'CATG_A
Cuts at: 0 1424 1554
Size: 1424 130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 257 1094 1300 1554
Size: 257 837 206 254
Fragments arranged by size:
837 257 254 206
BsrBI CCG'CTC
Cuts at: 0 416 1378 1554
Size: 416 962 176
Fragments arranged by size:
962 416 176
BsrDI GCAATG_nn'
Cuts at: 0 527 1554
Size: 527 1027
BsrFI r'CCGG_y
Cuts at: 0 703 1159 1554
Size: 703 456 395
Fragments arranged by size:
703 456 395
BssHII G'CGCG_C
Cuts at: 0 54 1554
Size: 54 1500
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1306 1315 1554
Size: 1306 9 239
Fragments arranged by size:
1306 239 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 231 1233 1554
Size: 231 1002 321
Fragments arranged by size:
1002 321 231
BstYI r'GATC_y
Cuts at: 0 721 1554
Size: 721 833
BstZ17I GTA'TAC
Cuts at: 0 794 1554
Size: 794 760
Bsu36I CC'TnA_GG
Cuts at: 0 1508 1554
Size: 1508 46
BtgI C'CryG_G
Cuts at: 0 107 212 236 267 350 1226 1554
Size: 107 105 24 31 83 876 328
Fragments arranged by size:
876 328 107 105 83 31 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1528 1554
Size: 1528 26
BtsI GCAGTG_nn'
Cuts at: 0 1325 1554
Size: 1325 229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 522 557 1554
Size: 522 35 997
Fragments arranged by size:
997 522 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1510 1554
Size: 1510 44
EaeI y'GGCC_r
Cuts at: 0 104 110 233 264 1554
Size: 104 6 123 31 1290
Fragments arranged by size:
1290 123 104 31 6
EarI CTCTTCn'nnn_
Cuts at: 0 803 1264 1554
Size: 803 461 290
Fragments arranged by size:
803 461 290
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 301 652 1083 1554
Size: 211 90 351 431 471
Fragments arranged by size:
471 431 351 211 90
EcoO109I rG'GnC_Cy
Cuts at: 0 142 149 296 1016 1554
Size: 142 7 147 720 538
Fragments arranged by size:
720 538 147 142 7
EcoRI G'AATT_C
Cuts at: 0 991 1038 1554
Size: 991 47 516
Fragments arranged by size:
991 516 47
FseI GG_CCGG'CC
Cuts at: 0 1163 1554
Size: 1163 391
HaeII r_GCGC'y
Cuts at: 0 22 165 184 221 1554
Size: 22 143 19 37 1333
Fragments arranged by size:
1333 143 37 22 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1081 1113 1513 1545 1554
Size: 1081 32 400 32 9
Fragments arranged by size:
1081 400 32 32 9
HincII GTy'rAC
Cuts at: 0 327 525 1554
Size: 327 198 1029
Fragments arranged by size:
1029 327 198
KasI G'GCGC_C
Cuts at: 0 217 1554
Size: 217 1337
MscI TGG'CCA
Cuts at: 0 235 1554
Size: 235 1319
MslI CAynn'nnrTG
Cuts at: 0 691 1231 1554
Size: 691 540 323
Fragments arranged by size:
691 540 323
MspA1I CmG'CkG
Cuts at: 0 109 214 269 1023 1554
Size: 109 105 55 754 531
Fragments arranged by size:
754 531 109 105 55
NaeI GCC'GGC
Cuts at: 0 705 1161 1554
Size: 705 456 393
Fragments arranged by size:
705 456 393
NarI GG'CG_CC
Cuts at: 0 218 1554
Size: 218 1336
NcoI C'CATG_G
Cuts at: 0 236 1554
Size: 236 1318
NgoMIV G'CCGG_C
Cuts at: 0 703 1159 1554
Size: 703 456 395
Fragments arranged by size:
703 456 395
PflMI CCAn_nnn'nTGG
Cuts at: 0 1403 1554
Size: 1403 151
PpuMI rG'GwC_Cy
Cuts at: 0 142 149 296 1016 1554
Size: 142 7 147 720 538
Fragments arranged by size:
720 538 147 142 7
PstI C_TGCA'G
Cuts at: 0 254 877 1320 1554
Size: 254 623 443 234
Fragments arranged by size:
623 443 254 234
PvuII CAG'CTG
Cuts at: 0 1023 1554
Size: 1023 531
SacII CC_GC'GG
Cuts at: 0 110 215 270 1554
Size: 110 105 55 1284
Fragments arranged by size:
1284 110 105 55
SanDI GG'GwC_CC
Cuts at: 0 142 149 296 1016 1554
Size: 142 7 147 720 538
Fragments arranged by size:
720 538 147 142 7
SbfI CC_TGCA'GG
Cuts at: 0 254 1554
Size: 254 1300
SfcI C'TryA_G
Cuts at: 0 250 313 873 1316 1554
Size: 250 63 560 443 238
Fragments arranged by size:
560 443 250 238 63
SfoI GGC'GCC
Cuts at: 0 219 1554
Size: 219 1335
SmaI CCC'GGG
Cuts at: 0 16 140 147 1554
Size: 16 124 7 1407
Fragments arranged by size:
1407 124 16 7
SrfI GCCC'GGGC
Cuts at: 0 16 1554
Size: 16 1538
StyI C'CwwG_G
Cuts at: 0 236 419 1051 1554
Size: 236 183 632 503
Fragments arranged by size:
632 503 236 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 742 1554
Size: 742 812
TatI w'GTAC_w
Cuts at: 0 443 1554
Size: 443 1111
Tth111I GACn'n_nGTC
Cuts at: 0 484 1014 1554
Size: 484 530 540
Fragments arranged by size:
540 530 484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1209 1554
Size: 1209 345
XmaI C'CCGG_G
Cuts at: 0 14 138 145 1554
Size: 14 124 7 1409
Fragments arranged by size:
1409 124 14 7
ZraI GAC'GTC
Cuts at: 0 432 1554
Size: 432 1122
Enzymes that do cut:
AarI AatII AccI AclI AflIII AlfI AlwNI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BmrI BplI
BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssHII BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DrdI EaeI EarI Eco57MI EcoO109I EcoRI
FseI HaeII Hin4I HincII KasI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV PflMI PpuMI PstI PvuII
SacII SanDI SbfI SfcI SfoI SmaI SrfI StyI
TaqII TatI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AcuI AfeI AflII AgeI AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmtI BpuEI BseRI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BsrGI BssSI BstBI BstEII ClaI DraI
DraIII EagI EciI EcoICRI EcoNI EcoRV FalI FspI
FspAI HindIII HpaI KpnI MfeI MluI MmeI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SalI SapI ScaI SexAI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SspI
StuI SwaI TstI XbaI XhoI XmnI