Restriction map for
pFN21AB5667
(Linear) (Six-base) MAPSORT of: KIBB5667.seq Check: 1278 from: 1 to: 1011
KIBB5667.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1011
Size: 295 716
AfeI AGC'GCT
Cuts at: 0 68 1011
Size: 68 943
AflII C'TTAA_G
Cuts at: 0 736 1011
Size: 736 275
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 949 1011
Size: 949 62
AlwNI CAG_nnn'CTG
Cuts at: 0 24 1011
Size: 24 987
ApaI G_GGCC'C
Cuts at: 0 124 183 801 1011
Size: 124 59 618 210
Fragments arranged by size:
618 210 124 59
ApoI r'AATT_y
Cuts at: 0 316 510 1011
Size: 316 194 501
Fragments arranged by size:
501 316 194
BanI G'GyrC_C
Cuts at: 0 40 205 637 1011
Size: 40 165 432 374
Fragments arranged by size:
432 374 165 40
BanII G_rGCy'C
Cuts at: 0 124 183 366 801 1011
Size: 124 59 183 435 210
Fragments arranged by size:
435 210 183 124 59
BbeI G_GCGC'C
Cuts at: 0 209 1011
Size: 209 802
BbvCI CC'TCA_GC
Cuts at: 0 473 1011
Size: 473 538
BfrBI ATG'CAT
Cuts at: 0 996 1011
Size: 996 15
BglI GCCn_nnn'nGGC
Cuts at: 0 244 919 1011
Size: 244 675 92
Fragments arranged by size:
675 244 92
Bme1580I G_kGCm'C
Cuts at: 0 43 58 124 183 801 870 1011
Size: 43 15 66 59 618 69 141
Fragments arranged by size:
618 141 69 66 59 43 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 95 846 1011
Size: 95 751 165
Fragments arranged by size:
751 165 95
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 74 903 1011
Size: 74 829 108
Fragments arranged by size:
829 108 74
Bpu10I CC'TnA_GC
Cuts at: 0 473 1011
Size: 473 538
BsaHI Gr'CG_yC
Cuts at: 0 206 1011
Size: 206 805
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 270 498 1011
Size: 270 228 513
Fragments arranged by size:
513 270 228
BseYI C'CCAG_C
Cuts at: 0 976 1011
Size: 976 35
BsmI GAATG_Cn'
Cuts at: 0 996 1011
Size: 996 15
Bsp1286I G_dGCh'C
Cuts at: 0 43 58 124 183 366 801 870 1011
Size: 43 15 66 59 183 435 69 141
Fragments arranged by size:
435 183 141 69 66 59 43 15
BsrBI CCG'CTC
Cuts at: 0 897 1011
Size: 897 114
BsrFI r'CCGG_y
Cuts at: 0 211 1011
Size: 211 800
BstAPI GCAn_nnn'nTGC
Cuts at: 0 24 499 858 1011
Size: 24 475 359 153
Fragments arranged by size:
475 359 153 24
BtgI C'CryG_G
Cuts at: 0 92 239 248 339 1011
Size: 92 147 9 91 672
Fragments arranged by size:
672 147 92 91 9
BtsI GCAGTG_nn'
Cuts at: 0 230 1011
Size: 230 781
EaeI y'GGCC_r
Cuts at: 0 285 1011
Size: 285 726
EarI CTCTTCn'nnn_
Cuts at: 0 559 1011
Size: 559 452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1011
Size: 295 716
EcoO109I rG'GnC_Cy
Cuts at: 0 121 179 180 579 798 1011
Size: 121 58 1 399 219 213
Fragments arranged by size:
399 219 213 121 58 1
EcoRI G'AATT_C
Cuts at: 0 316 510 1011
Size: 316 194 501
Fragments arranged by size:
501 316 194
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 979 1011
Size: 979 32
HaeII r_GCGC'y
Cuts at: 0 70 209 1011
Size: 70 139 802
Fragments arranged by size:
802 139 70
KasI G'GCGC_C
Cuts at: 0 205 1011
Size: 205 806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 662 686 1011
Size: 416 246 24 325
Fragments arranged by size:
416 325 246 24
MslI CAynn'nnrTG
Cuts at: 0 725 1011
Size: 725 286
MspA1I CmG'CkG
Cuts at: 0 21 47 94 165 1011
Size: 21 26 47 71 846
Fragments arranged by size:
846 71 47 26 21
NaeI GCC'GGC
Cuts at: 0 213 1011
Size: 213 798
NarI GG'CG_CC
Cuts at: 0 206 1011
Size: 206 805
NgoMIV G'CCGG_C
Cuts at: 0 211 1011
Size: 211 800
NsiI A_TGCA'T
Cuts at: 0 998 1011
Size: 998 13
PflMI CCAn_nnn'nTGG
Cuts at: 0 825 1011
Size: 825 186
PfoI T'CCnGG_A
Cuts at: 0 689 1011
Size: 689 322
PspOMI G'GGCC_C
Cuts at: 0 120 179 797 1011
Size: 120 59 618 214
Fragments arranged by size:
618 214 120 59
PstI C_TGCA'G
Cuts at: 0 29 1011
Size: 29 982
SacII CC_GC'GG
Cuts at: 0 95 1011
Size: 95 916
SfcI C'TryA_G
Cuts at: 0 25 1011
Size: 25 986
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 244 1011
Size: 244 767
SfoI GGC'GCC
Cuts at: 0 207 1011
Size: 207 804
SmlI C'TyrA_G
Cuts at: 0 736 1011
Size: 736 275
StyI C'CwwG_G
Cuts at: 0 830 1011
Size: 830 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 828 1011
Size: 828 183
XbaI T'CTAG_A
Cuts at: 0 874 1011
Size: 874 137
Enzymes that do cut:
AcuI AfeI AflII AlfI AlwNI ApaI ApoI BanI
BanII BbeI BbvCI BfrBI BglI Bme1580I BmrI BplI
Bpu10I BsaHI BseRI BseYI BsmI Bsp1286I BsrBI BsrFI
BstAPI BtgI BtsI EaeI EarI Eco57MI EcoO109I EcoRI
FalI HaeII KasI MmeI MslI MspA1I NaeI NarI
NgoMIV NsiI PflMI PfoI PspOMI PstI SacII SfcI
SfiI SfoI SmlI StyI TaqII XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbsI BcgI BciVI BclI BglII BlpI
BmgBI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaWI
BsaXI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI
BspMI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRV FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MscI NcoI NdeI NheI NotI NruI NspI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
Tth111I XcmI XhoI XmaI XmnI ZraI