Restriction map for
pFN21AB5621
(Linear) (Six-base) MAPSORT of: KIBB5621.seq Check: 1825 from: 1 to: 4068
KIBB5621.orf
With 182 enzymes: SgfI *
March 22, 2012 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2215 4068
Size: 2215 1853
AatII G_ACGT'C
Cuts at: 0 3263 4068
Size: 3263 805
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2410 2521 2996 3908 4068
Size: 2410 111 475 912 160
Fragments arranged by size:
2410 912 475 160 111
AflII C'TTAA_G
Cuts at: 0 1885 4068
Size: 1885 2183
AflIII A'CryG_T
Cuts at: 0 473 1330 4068
Size: 473 857 2738
Fragments arranged by size:
2738 857 473
AgeI A'CCGG_T
Cuts at: 0 4006 4068
Size: 4006 62
AhdI GACnn_n'nnGTC
Cuts at: 0 1646 3264 4068
Size: 1646 1618 804
Fragments arranged by size:
1646 1618 804
AleI CACnn'nnGTG
Cuts at: 0 2314 3949 4068
Size: 2314 1635 119
Fragments arranged by size:
2314 1635 119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 31 3515 4068
Size: 31 3484 553
Fragments arranged by size:
3484 553 31
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2645 2677 4068
Size: 2645 32 1391
Fragments arranged by size:
2645 1391 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1770 3669 4068
Size: 1770 1899 399
Fragments arranged by size:
1899 1770 399
ApaI G_GGCC'C
Cuts at: 0 3381 4068
Size: 3381 687
ApaLI G'TGCA_C
Cuts at: 0 1710 4068
Size: 1710 2358
ApoI r'AATT_y
Cuts at: 0 527 859 2482 2749 2758 2800 3340 3631
Size: 527 332 1623 267 9 42 540 291
Cuts at: 3631 4068
Size: 437
Fragments arranged by size:
1623 540 527 437 332 291 267 42
9
AseI AT'TA_AT
Cuts at: 0 611 4068
Size: 611 3457
AvaI C'yCGr_G
Cuts at: 0 50 451 3149 4038 4068
Size: 50 401 2698 889 30
Fragments arranged by size:
2698 889 401 50 30
BamHI G'GATC_C
Cuts at: 0 2418 4068
Size: 2418 1650
BanI G'GyrC_C
Cuts at: 0 1312 1995 2266 2527 2707 3855 4068
Size: 1312 683 271 261 180 1148 213
Fragments arranged by size:
1312 1148 683 271 261 213 180
BanII G_rGCy'C
Cuts at: 0 1409 2643 2914 3381 3440 4057 4068
Size: 1409 1234 271 467 59 617 11
Fragments arranged by size:
1409 1234 617 467 271 59 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 585 1948 2132 2398 3824 4068
Size: 585 1363 184 266 1426 244
Fragments arranged by size:
1426 1363 585 266 244 184
BciVI GTATCCnnnnn_n'
Cuts at: 0 1812 3082 4068
Size: 1812 1270 986
Fragments arranged by size:
1812 1270 986
BclI T'GATC_A
Cuts at: 0 572 1874 4068
Size: 572 1302 2194
Fragments arranged by size:
2194 1302 572
BfrBI ATG'CAT
Cuts at: 0 1894 4068
Size: 1894 2174
BglI GCCn_nnn'nGGC
Cuts at: 0 2727 4068
Size: 2727 1341
BglII A'GATC_T
Cuts at: 0 364 1723 2979 4068
Size: 364 1359 1256 1089
Fragments arranged by size:
1359 1256 1089 364
BlpI GC'TnA_GC
Cuts at: 0 100 2913 3503 4068
Size: 100 2813 590 565
Fragments arranged by size:
2813 590 565 100
Bme1580I G_kGCm'C
Cuts at: 0 1714 2271 2829 3381 3860 4068
Size: 1714 557 558 552 479 208
Fragments arranged by size:
1714 558 557 552 479 208
BmrI ACTGGGnnnn_n'
Cuts at: 0 786 831 3434 4068
Size: 786 45 2603 634
Fragments arranged by size:
2603 786 634 45
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 707 2749 4068
Size: 707 2042 1319
Fragments arranged by size:
2042 1319 707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 2038 3942 4012 4068
Size: 314 1724 1904 70 56
Fragments arranged by size:
1904 1724 314 70 56
Bpu10I CC'TnA_GC
Cuts at: 0 3038 4068
Size: 3038 1030
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1132 1916 2641 4068
Size: 1132 784 725 1427
Fragments arranged by size:
1427 1132 784 725
BsaI GGTCTCn'nnnn_
Cuts at: 0 40 818 1242 2107 2486 3441 4068
Size: 40 778 424 865 379 955 627
Fragments arranged by size:
955 865 778 627 424 379 40
BsaAI yAC'GTr
Cuts at: 0 1638 4068
Size: 1638 2430
BsaHI Gr'CG_yC
Cuts at: 0 3260 4068
Size: 3260 808
BsaWI w'CCGG_w
Cuts at: 0 2156 3919 4006 4068
Size: 2156 1763 87 62
Fragments arranged by size:
2156 1763 87 62
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1378 1408 1607 1637 1684 1714 1904 1934
Size: 1378 30 199 30 47 30 190 30
Cuts at: 1934 2926 2956 4068
Size: 992 30 1112
Fragments arranged by size:
1378 1112 992 199 190 47 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1628 2799 2950 3622 3625 4049 4068
Size: 1628 1171 151 672 3 424 19
Fragments arranged by size:
1628 1171 672 424 151 19 3
BseYI C'CCAG_C
Cuts at: 0 562 1408 3859 4068
Size: 562 846 2451 209
Fragments arranged by size:
2451 846 562 209
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 941 4068
Size: 255 686 3127
Fragments arranged by size:
3127 686 255
BsiHKAI G_wGCw'C
Cuts at: 0 871 1714 2643 2914 4057 4068
Size: 871 843 929 271 1143 11
Fragments arranged by size:
1143 929 871 843 271 11
BsmI GAATG_Cn'
Cuts at: 0 1842 1896 4068
Size: 1842 54 2172
Fragments arranged by size:
2172 1842 54
BsmBI CGTCTCn'nnnn_
Cuts at: 0 695 4068
Size: 695 3373
Bsp1286I G_dGCh'C
Cuts at: 0 871 1409 1714 2271 2643 2829 2914 3381
Size: 871 538 305 557 372 186 85 467
Cuts at: 3381 3440 3860 4057 4068
Size: 59 420 197 11
Fragments arranged by size:
871 557 538 467 420 372 305 197
186 85 59 11
BspEI T'CCGG_A
Cuts at: 0 3919 4068
Size: 3919 149
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2215 4068
Size: 2215 1853
BsrDI GCAATG_nn'
Cuts at: 0 1956 4068
Size: 1956 2112
BsrFI r'CCGG_y
Cuts at: 0 2495 3692 4006 4068
Size: 2495 1197 314 62
Fragments arranged by size:
2495 1197 314 62
BsrGI T'GTAC_A
Cuts at: 0 407 638 736 911 1580 3242 4068
Size: 407 231 98 175 669 1662 826
Fragments arranged by size:
1662 826 669 407 231 175 98
BstBI TT'CG_AA
Cuts at: 0 793 4068
Size: 793 3275
BstEII G'GTnAC_C
Cuts at: 0 1066 2677 4068
Size: 1066 1611 1391
Fragments arranged by size:
1611 1391 1066
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2415 4068
Size: 2415 1653
BstYI r'GATC_y
Cuts at: 0 364 1723 2418 2660 2871 2979 3165 4068
Size: 364 1359 695 242 211 108 186 903
Fragments arranged by size:
1359 903 695 364 242 211 186 108
Bsu36I CC'TnA_GG
Cuts at: 0 2782 3300 4068
Size: 2782 518 768
Fragments arranged by size:
2782 768 518
BtgI C'CryG_G
Cuts at: 0 1025 2523 4068
Size: 1025 1498 1545
Fragments arranged by size:
1545 1498 1025
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 254 3044 4068
Size: 254 2790 1024
Fragments arranged by size:
2790 1024 254
BtsI GCAGTG_nn'
Cuts at: 0 213 1712 2226 4068
Size: 213 1499 514 1842
Fragments arranged by size:
1842 1499 514 213
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1357 1392 1763 1798 2849 2884 4068
Size: 1357 35 371 35 1051 35 1184
Fragments arranged by size:
1357 1184 1051 371 35 35 35
DraI TTT'AAA
Cuts at: 0 2103 3980 4068
Size: 2103 1877 88
Fragments arranged by size:
2103 1877 88
DraIII CAC_nnn'GTG
Cuts at: 0 484 2629 4068
Size: 484 2145 1439
Fragments arranged by size:
2145 1439 484
EaeI y'GGCC_r
Cuts at: 0 20 2520 2535 4068
Size: 20 2500 15 1533
Fragments arranged by size:
2500 1533 20 15
EarI CTCTTCn'nnn_
Cuts at: 0 149 253 1385 2957 3554 3794 4068
Size: 149 104 1132 1572 597 240 274
Fragments arranged by size:
1572 1132 597 274 240 149 104
EcoICRI GAG'CTC
Cuts at: 0 2641 2912 4055 4068
Size: 2641 271 1143 13
Fragments arranged by size:
2641 1143 271 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 2038 2410 2521 2996 3908 3942 4012
Size: 314 1724 372 111 475 912 34 70
Cuts at: 4012 4068
Size: 56
Fragments arranged by size:
1724 912 475 372 314 111 70 56
34
EcoO109I rG'GnC_Cy
Cuts at: 0 1648 3081 3377 3378 4068
Size: 1648 1433 296 1 690
Fragments arranged by size:
1648 1433 690 296 1
EcoRI G'AATT_C
Cuts at: 0 2482 2749 4068
Size: 2482 267 1319
Fragments arranged by size:
2482 1319 267
EcoRV GAT'ATC
Cuts at: 0 3539 3723 3925 4068
Size: 3539 184 202 143
Fragments arranged by size:
3539 202 184 143
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 136 567 744 2301 3991 4068
Size: 136 431 177 1557 1690 77
Fragments arranged by size:
1690 1557 431 177 136 77
HaeII r_GCGC'y
Cuts at: 0 2886 4068
Size: 2886 1182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 316 348 349 381 432 464 1632 1664
Size: 316 32 1 32 51 32 1168 32
Cuts at: 1664 1684 1716 1904 1936 2926 2958 3250 3282
Size: 20 32 188 32 990 32 292 32
Cuts at: 3282 4068
Size: 786
Fragments arranged by size:
1168 990 786 316 292 188 51 32
32 32 32 32 32 32 32 20
1
HincII GTy'rAC
Cuts at: 0 1599 2697 4068
Size: 1599 1098 1371
Fragments arranged by size:
1599 1371 1098
HindIII A'AGCT_T
Cuts at: 0 721 1753 4068
Size: 721 1032 2315
Fragments arranged by size:
2315 1032 721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 693 1364 2835 4068
Size: 693 671 1471 1233
Fragments arranged by size:
1471 1233 693 671
MscI TGG'CCA
Cuts at: 0 2537 4068
Size: 2537 1531
MslI CAynn'nnrTG
Cuts at: 0 2314 2413 2687 3932 3949 4068
Size: 2314 99 274 1245 17 119
Fragments arranged by size:
2314 1245 274 119 99 17
NaeI GCC'GGC
Cuts at: 0 3694 4068
Size: 3694 374
NgoMIV G'CCGG_C
Cuts at: 0 3692 4068
Size: 3692 376
NruI TCG'CGA
Cuts at: 0 3063 4068
Size: 3063 1005
NsiI A_TGCA'T
Cuts at: 0 1896 4068
Size: 1896 2172
NspI r_CATG'y
Cuts at: 0 1144 1334 1677 2219 4068
Size: 1144 190 343 542 1849
Fragments arranged by size:
1849 1144 542 343 190
PciI A'CATG_T
Cuts at: 0 1330 4068
Size: 1330 2738
PflMI CCAn_nnn'nTGG
Cuts at: 0 2477 3319 3844 4068
Size: 2477 842 525 224
Fragments arranged by size:
2477 842 525 224
PmlI CAC'GTG
Cuts at: 0 1638 4068
Size: 1638 2430
PpuMI rG'GwC_Cy
Cuts at: 0 1648 3081 4068
Size: 1648 1433 987
Fragments arranged by size:
1648 1433 987
PsiI TTA'TAA
Cuts at: 0 3161 4068
Size: 3161 907
PspOMI G'GGCC_C
Cuts at: 0 3377 4068
Size: 3377 691
PstI C_TGCA'G
Cuts at: 0 1719 3674 4068
Size: 1719 1955 394
Fragments arranged by size:
1955 1719 394
RsrII CG'GwC_CG
Cuts at: 0 2360 4068
Size: 2360 1708
SacI G_AGCT'C
Cuts at: 0 2643 2914 4057 4068
Size: 2643 271 1143 11
Fragments arranged by size:
2643 1143 271 11
SapI GCTCTTCn'nnn_
Cuts at: 0 3794 4068
Size: 3794 274
SfcI C'TryA_G
Cuts at: 0 878 1715 3670 4068
Size: 878 837 1955 398
Fragments arranged by size:
1955 878 837 398
SmaI CCC'GGG
Cuts at: 0 52 3151 4068
Size: 52 3099 917
Fragments arranged by size:
3099 917 52
SmlI C'TyrA_G
Cuts at: 0 1111 1885 1931 2656 4068
Size: 1111 774 46 725 1412
Fragments arranged by size:
1412 1111 774 725 46
SphI G_CATG'C
Cuts at: 0 2219 4068
Size: 2219 1849
SspI AAT'ATT
Cuts at: 0 3293 4068
Size: 3293 775
StuI AGG'CCT
Cuts at: 0 2198 4068
Size: 2198 1870
StyI C'CwwG_G
Cuts at: 0 903 1062 1908 3002 4068
Size: 903 159 846 1094 1066
Fragments arranged by size:
1094 1066 903 846 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 324 2013 4068
Size: 324 1689 2055
Fragments arranged by size:
2055 1689 324
TatI w'GTAC_w
Cuts at: 0 407 638 736 911 1143 1580 3242 3953
Size: 407 231 98 175 232 437 1662 711
Cuts at: 3953 4068
Size: 115
Fragments arranged by size:
1662 711 437 407 232 231 175 115
98
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1378 1410 1607 1639 3083 3115 4068
Size: 1378 32 197 32 1444 32 953
Fragments arranged by size:
1444 1378 953 197 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1918 4068
Size: 1918 2150
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1085 2093 3775 4068
Size: 1085 1008 1682 293
Fragments arranged by size:
1682 1085 1008 293
XmaI C'CCGG_G
Cuts at: 0 50 3149 4068
Size: 50 3099 919
Fragments arranged by size:
3099 919 50
ZraI GAC'GTC
Cuts at: 0 3261 4068
Size: 3261 807
Enzymes that do cut:
AarI AatII AcuI AflII AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AvaI
BamHI BanI BanII BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI
BsrGI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII EaeI EarI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
MmeI MscI MslI NaeI NgoMIV NruI NsiI NspI
PciI PflMI PmlI PpuMI PsiI PspOMI PstI RsrII
SacI SapI SfcI SmaI SmlI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AscI AsiSI AvrII BaeI
BbeI BbvCI BcgI BmgBI BmtI BsaBI BsiEI BsiWI
BspHI BsrBI BssHII BssSI BstAPI BstZ17I ClaI DrdI
EagI EciI EcoNI FseI FspI FspAI HpaI KasI
KpnI MfeI MluI MspA1I NarI NcoI NdeI NheI
NotI PacI PasI PfoI PmeI PpiI PshAI PspXI
PsrI PvuI PvuII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SwaI XbaI XhoI XmnI