Restriction map for
pFN21AB5592
(Linear) (Six-base) MAPSORT of: KIBB5592.seq Check: 9945 from: 1 to: 1482
KIBB5592.orf
With 182 enzymes: SgfI *
June 18, 2013 11:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 470 1048 1482
Size: 470 578 434
Fragments arranged by size:
578 470 434
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1334 1482
Size: 1334 148
AflIII A'CryG_T
Cuts at: 0 466 1141 1482
Size: 466 675 341
Fragments arranged by size:
675 466 341
AlwNI CAG_nnn'CTG
Cuts at: 0 1361 1482
Size: 1361 121
ApaI G_GGCC'C
Cuts at: 0 260 1482
Size: 260 1222
ApoI r'AATT_y
Cuts at: 0 432 526 649 1482
Size: 432 94 123 833
Fragments arranged by size:
833 432 123 94
AvaI C'yCGr_G
Cuts at: 0 128 171 1269 1482
Size: 128 43 1098 213
Fragments arranged by size:
1098 213 128 43
BanI G'GyrC_C
Cuts at: 0 109 1482
Size: 109 1373
BanII G_rGCy'C
Cuts at: 0 260 1482
Size: 260 1222
BbeI G_GCGC'C
Cuts at: 0 113 1482
Size: 113 1369
BbsI GAAGACnn'nnnn_
Cuts at: 0 1112 1482
Size: 1112 370
BbvCI CC'TCA_GC
Cuts at: 0 959 1482
Size: 959 523
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 234 268 1482
Size: 234 34 1214
Fragments arranged by size:
1214 234 34
BfrBI ATG'CAT
Cuts at: 0 554 1450 1482
Size: 554 896 32
Fragments arranged by size:
896 554 32
Bme1580I G_kGCm'C
Cuts at: 0 260 1482
Size: 260 1222
BmgBI CAC'GTC
Cuts at: 0 695 1482
Size: 695 787
BmrI ACTGGGnnnn_n'
Cuts at: 0 782 789 1482
Size: 782 7 693
Fragments arranged by size:
782 693 7
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 1034 1482
Size: 683 351 448
Fragments arranged by size:
683 448 351
Bpu10I CC'TnA_GC
Cuts at: 0 959 1482
Size: 959 523
BsaI GGTCTCn'nnnn_
Cuts at: 0 283 1384 1482
Size: 283 1101 98
Fragments arranged by size:
1101 283 98
BsaAI yAC'GTr
Cuts at: 0 231 1482
Size: 231 1251
BsaBI GATnn'nnATC
Cuts at: 0 764 1482
Size: 764 718
BsaHI Gr'CG_yC
Cuts at: 0 37 110 215 1482
Size: 37 73 105 1267
Fragments arranged by size:
1267 105 73 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 294 324 1482
Size: 294 30 1158
Fragments arranged by size:
1158 294 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 111 976 1482
Size: 111 865 506
Fragments arranged by size:
865 506 111
BseYI C'CCAG_C
Cuts at: 0 55 1427 1482
Size: 55 1372 55
Fragments arranged by size:
1372 55 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 930 1482
Size: 332 598 552
Fragments arranged by size:
598 552 332
BsiEI CG_ry'CG
Cuts at: 0 195 1482
Size: 195 1287
BsiHKAI G_wGCw'C
Cuts at: 0 307 935 1270 1482
Size: 307 628 335 212
Fragments arranged by size:
628 335 307 212
BsmI GAATG_Cn'
Cuts at: 0 556 1429 1482
Size: 556 873 53
Fragments arranged by size:
873 556 53
BsmBI CGTCTCn'nnnn_
Cuts at: 0 701 1482
Size: 701 781
Bsp1286I G_dGCh'C
Cuts at: 0 260 307 935 1270 1482
Size: 260 47 628 335 212
Fragments arranged by size:
628 335 260 212 47
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 451 470 1048 1482
Size: 451 19 578 434
Fragments arranged by size:
578 451 434 19
BsrGI T'GTAC_A
Cuts at: 0 1387 1482
Size: 1387 95
BstAPI GCAn_nnn'nTGC
Cuts at: 0 713 1265 1482
Size: 713 552 217
Fragments arranged by size:
713 552 217
BstYI r'GATC_y
Cuts at: 0 993 1482
Size: 993 489
BtgI C'CryG_G
Cuts at: 0 189 318 588 1134 1201 1482
Size: 189 129 270 546 67 281
Fragments arranged by size:
546 281 270 189 129 67
DrdI GACnn_nn'nnGTC
Cuts at: 0 214 1482
Size: 214 1268
EaeI y'GGCC_r
Cuts at: 0 103 192 1482
Size: 103 89 1290
Fragments arranged by size:
1290 103 89
EagI C'GGCC_G
Cuts at: 0 192 1482
Size: 192 1290
EarI CTCTTCn'nnn_
Cuts at: 0 1310 1482
Size: 1310 172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 1034 1334 1482
Size: 683 351 300 148
Fragments arranged by size:
683 351 300 148
EcoO109I rG'GnC_Cy
Cuts at: 0 257 1482
Size: 257 1225
EcoRI G'AATT_C
Cuts at: 0 432 649 1482
Size: 432 217 833
Fragments arranged by size:
833 432 217
EcoRV GAT'ATC
Cuts at: 0 663 1482
Size: 663 819
HaeII r_GCGC'y
Cuts at: 0 113 1482
Size: 113 1369
HindIII A'AGCT_T
Cuts at: 0 78 1482
Size: 78 1404
KasI G'GCGC_C
Cuts at: 0 109 1482
Size: 109 1373
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 793 1482
Size: 119 674 689
Fragments arranged by size:
689 674 119
MspA1I CmG'CkG
Cuts at: 0 67 203 958 1100 1203 1482
Size: 67 136 755 142 103 279
Fragments arranged by size:
755 279 142 136 103 67
NarI GG'CG_CC
Cuts at: 0 110 1482
Size: 110 1372
NcoI C'CATG_G
Cuts at: 0 318 588 1482
Size: 318 270 894
Fragments arranged by size:
894 318 270
NsiI A_TGCA'T
Cuts at: 0 556 1452 1482
Size: 556 896 30
Fragments arranged by size:
896 556 30
NspI r_CATG'y
Cuts at: 0 249 470 1145 1454 1482
Size: 249 221 675 309 28
Fragments arranged by size:
675 309 249 221 28
PasI CC'CwG_GG
Cuts at: 0 671 1482
Size: 671 811
PciI A'CATG_T
Cuts at: 0 466 1141 1482
Size: 466 675 341
Fragments arranged by size:
675 466 341
PfoI T'CCnGG_A
Cuts at: 0 1050 1482
Size: 1050 432
PmlI CAC'GTG
Cuts at: 0 231 1482
Size: 231 1251
PspOMI G'GGCC_C
Cuts at: 0 256 1482
Size: 256 1226
PstI C_TGCA'G
Cuts at: 0 121 718 1482
Size: 121 597 764
Fragments arranged by size:
764 597 121
PvuII CAG'CTG
Cuts at: 0 67 958 1482
Size: 67 891 524
Fragments arranged by size:
891 524 67
SacII CC_GC'GG
Cuts at: 0 1204 1482
Size: 1204 278
SbfI CC_TGCA'GG
Cuts at: 0 121 1482
Size: 121 1361
SfcI C'TryA_G
Cuts at: 0 117 714 825 1482
Size: 117 597 111 657
Fragments arranged by size:
657 597 117 111
SfoI GGC'GCC
Cuts at: 0 111 1482
Size: 111 1371
SmaI CCC'GGG
Cuts at: 0 130 173 1482
Size: 130 43 1309
Fragments arranged by size:
1309 130 43
StyI C'CwwG_G
Cuts at: 0 318 588 1482
Size: 318 270 894
Fragments arranged by size:
894 318 270
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 270 857 950 1482
Size: 270 587 93 532
Fragments arranged by size:
587 532 270 93
TatI w'GTAC_w
Cuts at: 0 1387 1482
Size: 1387 95
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 294 326 912 925 944 957 1029 1061
Size: 294 32 586 13 19 13 72 32
Cuts at: 1061 1482
Size: 421
Fragments arranged by size:
586 421 294 72 32 32 19 13
13
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 361 1482
Size: 361 1121
XmaI C'CCGG_G
Cuts at: 0 128 171 1482
Size: 128 43 1311
Fragments arranged by size:
1311 128 43
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BfrBI Bme1580I BmgBI
BmrI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrGI BstAPI BstYI BtgI DrdI EaeI EagI
EarI Eco57MI EcoO109I EcoRI EcoRV HaeII HindIII KasI
MmeI MspA1I NarI NcoI NsiI NspI PasI PciI
PfoI PmlI PspOMI PstI PvuII SacII SbfI SfcI
SfoI SmaI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BciVI BclI BglI BglII BlpI BmtI
BplI BpuEI BsaWI BsiWI BspEI BspHI BsrBI BsrDI
BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII EciI EcoICRI
EcoNI FalI FseI FspI FspAI Hin4I HincII HpaI
KpnI MfeI MluI MscI MslI NaeI NdeI NgoMIV
NheI NotI NruI PacI PflMI PmeI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SanDI SapI ScaI SexAI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XhoI XmnI ZraI